Rh4CG464100

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002622
Physical Location & Seq
Forward (+)
69420996 .. 69423440
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG464100.1

Sequence Viewer

Length: 1323 bp
ATGGGTTCCTTGTCTGGAATCCTTCAACGCCCTCTAATCGCCGCCGCCGCTGTTGGCTTTGCTTCTTTTTCTTCCGACTTCTCCCTCATACTGCCCTCTCACAAATCCAAATCCAAATCCAACGACTCCTCCTCCTCGGATTTATCAACTTCAACAACAACAACTTTGTCATCGGACATTTCGCTTTCTAAGCTCGCAAACCTCTCCTTTGTCTCCAGAATTCAAGTACCTGTTCCCACCATTAACTTCCCAATTCCCAATTCCCACTGCAATTCCCTCTATTCTTCGGTTGCATCTTCCCCCGCTCTTCTTAAGCTCTATCACTCTGCACACTTGGCGGCCAGAGCGCCCAACCCAACTCCATATAAACATGATACAATATCATCATCTGCTTCCGTCCCTGACGTCTCCTACACATGGCATTTGCCTGAGCCAAATGCTCTTGATGTTTCCCGCACCTCTGATTGTTCATCAGCAGCAAAGATGAGGACTGTGGTGGTTTTGCTGGGATGGTTAGGAGCAAAGCAAAAGCATCTCAAGAGGTATGCCGACTGGTATACTTCCCAGGGGTTTCACGTCATTACCTTTACTTTACCCATGGCTGACATTCTCAAATACCAGCCTGGTGGTAAAGTCGAAGACCACATTGACCTGCTTGTCACTCATTTGGCTGATTGGTTAGAAGAGGAGCAGGGAAAGAACCTGCTTTTTCATACTTTTAGCAACACCGGATGGTTAACGTATGGTGTAATGTTGGAGAAGTTCCAGAAGCACGATCCTTCTGTAATGGGCAGGATTCGGGGCTGCGTTGTGGATTCCGCACCTGTTGCAGCACCTGACCCGCAGGTCTGGGCTTCTGGTTTCTCTGCAGCCTTTCTGAAAAAGCATAGTGTAGCAACGAAAGGAACCACAGACGCTAGTGAGTCAGGGATGGATGCTTTGGTTGACAGCAAGGAAGTAGTGGCACCCAAACCTGCAGTAACTGAGGCAGCTTTGCTTTTAGTTTTGGAGAAGTTCTTTGAGGTGGTTTTGAATCTTCCTACAGTGAACAGGAGGCTTTCTGATGTTTTGGGCTTACTGTCAGCACGACAACCAAGCTGTCCGCAATTATATATCTACAGCTCTGCAGACAGAGTCATTCCTGCAGGATCTGTGGAGTCATTCATAAAACAACAGCGGAGGGCTGGACATGAGGTTAGGGCTTGCAACTTTGTCTCGACACCTCATGTTGATCATTTCAGAAATGACCCGAAGCTGTATACTTCTGAGCTAACTCACTTTCTGGAGGACTGTGTGCTTACTTGTTGCAAACAGTCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

440

Amino Acids

47.99

Weight (kDa)

8.06

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 164 - 428 1.4e-70 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 656, 845
AatII GACGTC 1 cut(s) 408
Acc36I ACCTGC 4 cut(s) 660, 711, 835, 982
AccB1I GGYRCC 1 cut(s) 964
AccBSI CCGCTC 1 cut(s) 305
AccI GTMKAC 2 cut(s) 557, 1259
AclWI GGATC 2 cut(s) 770, 1156
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 417
AflII CTTAAG 1 cut(s) 311
AgsI TTSAA 4 cut(s) 26, 153, 224, 1033
AjiI CACGTC 1 cut(s) 577
AjnI CCWGG 2 cut(s) 564, 622
AjuI GAANNNNNNNTTGG 2 cut(s) 244, 276
AluBI AGCT 7 cut(s) 193, 316, 992, 1098, 1122, 1255, 1270
AluI AGCT 7 cut(s) 193, 316, 992, 1098, 1122, 1255, 1270
Alw26I GTCTC 3 cut(s) 217, 412, 1219
AlwI GGATC 2 cut(s) 770, 1156
AlwNI CAGNNNCTG 3 cut(s) 836, 983, 1151
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 5 cut(s) 476, 804, 830, 869, 989
ApoI RAATTY 1 cut(s) 219
AspLEI GCGC 1 cut(s) 349
BanI GGYRCC 1 cut(s) 964
BbsI GAAGAC 1 cut(s) 645
BbvI GCAGC 5 cut(s) 488, 791, 842, 881, 1001
BccI CCATC 3 cut(s) 504, 726, 925
BciT130I CCWGG 2 cut(s) 566, 624
BclI TGATCA 1 cut(s) 1231
BcoDI GTCTC 3 cut(s) 217, 412, 1219
BfaI CTAG 1 cut(s) 918
BfmI CTRYAG 6 cut(s) 867, 975, 1041, 1117, 1125, 1143
BfoI RGCGCY 1 cut(s) 350
BfrI CTTAAG 1 cut(s) 311
BfuAI ACCTGC 4 cut(s) 660, 711, 835, 982
BisI GCNGC 9 cut(s) 42, 45, 48, 339, 477, 805, 831, 870, 990
BlsI GCNGC 9 cut(s) 43, 46, 49, 340, 478, 806, 832, 871, 991
Bme1390I CCNGG 2 cut(s) 566, 624
BmgBI CACGTC 1 cut(s) 577
BmiI GGNNCC 3 cut(s) 7, 907, 966
BmrFI CCNGG 2 cut(s) 566, 624
BmsI GCATC 3 cut(s) 302, 541, 925
BpiI GAAGAC 1 cut(s) 645
BpmI CTGGAG 2 cut(s) 199, 1304
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 4 cut(s) 135, 564, 565, 597
BsaWI WCCGGW 1 cut(s) 728
BsaXI ACNNNNNCTCC 4 cut(s) 113, 116, 143, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 557
BseBI CCWGG 2 cut(s) 566, 624
BseDI CCNNGG 4 cut(s) 135, 564, 565, 597
BseGI GGATG 4 cut(s) 515, 737, 936, 940
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMII CTCAG 3 cut(s) 420, 975, 1257
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 4 cut(s) 118, 121, 124, 701
BseXI GCAGC 5 cut(s) 488, 791, 842, 881, 1001
BseYI CCCAGC 1 cut(s) 505
BsgI GTGCAG 1 cut(s) 312
BshFI GGCC 1 cut(s) 341
BshNI GGYRCC 1 cut(s) 964
BsiSI CCGG 1 cut(s) 729
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 3 cut(s) 217, 412, 1219
BsmBI CGTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 1 cut(s) 341
Bsp143I GATC 3 cut(s) 775, 1148, 1231
Bsp19I CCATGG 1 cut(s) 597
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 3 cut(s) 421, 976, 1258
BspLI GGNNCC 3 cut(s) 7, 907, 966
BspMAI CTGCAG 4 cut(s) 871, 979, 1129, 1147
BspMI ACCTGC 4 cut(s) 660, 711, 835, 982
BspPI GGATC 2 cut(s) 770, 1156
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 964
BspTI CTTAAG 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 557
BssECI CCNNGG 4 cut(s) 135, 564, 565, 597
BssMI GATC 3 cut(s) 775, 1148, 1231
BssNAI GTATAC 2 cut(s) 558, 1260
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 597
Bst1107I GTATAC 2 cut(s) 558, 1260
Bst2UI CCWGG 2 cut(s) 566, 624
Bst4CI ACNGT 5 cut(s) 493, 1045, 1080, 1292, 1314
Bst6I CTCTTC 2 cut(s) 312, 678
BstACI GRCGYC 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 2 cut(s) 195, 1204
BstDEI CTNAG 4 cut(s) 189, 429, 984, 1266
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 4 cut(s) 515, 737, 936, 940
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 3 cut(s) 778, 1151, 1234
BstMAI GTCTC 3 cut(s) 217, 412, 1219
BstMBI GATC 3 cut(s) 775, 1148, 1231
BstMWI GCNNNNNNNGC 5 cut(s) 47, 190, 335, 344, 827
BstNI CCWGG 2 cut(s) 566, 624
BstSCI CCNGG 2 cut(s) 564, 622
BstSFI CTRYAG 6 cut(s) 867, 975, 1041, 1117, 1125, 1143
BstV1I GCAGC 5 cut(s) 488, 791, 842, 881, 1001
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 1 cut(s) 1148
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 1 cut(s) 1148
BstZ17I GTATAC 2 cut(s) 558, 1260
BsuRI GGCC 1 cut(s) 341
BtgI CCRYGG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 577
BtsCI GGATG 4 cut(s) 515, 737, 936, 940
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 2 cut(s) 265, 1050
BveI ACCTGC 4 cut(s) 660, 711, 835, 982
Cac8I GCNNGC 2 cut(s) 195, 1204
CaiI CAGNNNCTG 3 cut(s) 836, 983, 1151
CfoI GCGC 1 cut(s) 349
CseI GACGC 1 cut(s) 923
Csp6I GTAC 1 cut(s) 227
CviAII CATG 5 cut(s) 371, 417, 598, 1190, 1226
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 4 cut(s) 189, 429, 984, 1266
DpnI GATC 3 cut(s) 777, 1150, 1233
DpnII GATC 3 cut(s) 775, 1148, 1231
DrdI GACNNNNNNGTC 2 cut(s) 656, 845
DseDI GACNNNNNNGTC 2 cut(s) 656, 845
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 2 cut(s) 312, 678
EarI CTCTTC 2 cut(s) 312, 678
Eco130I CCWWGG 1 cut(s) 597
EcoRI GAATTC 1 cut(s) 219
EcoRII CCWGG 2 cut(s) 564, 622
EcoT14I CCWWGG 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 597
Esp3I CGTCTC 1 cut(s) 412
FaeI CATG 5 cut(s) 374, 420, 601, 1193, 1229
FaqI GGGAC 1 cut(s) 383
FatI CATG 5 cut(s) 370, 416, 597, 1189, 1225
FauI CCCGC 3 cut(s) 310, 461, 849
FbaI TGATCA 1 cut(s) 1231
FblI GTMKAC 2 cut(s) 557, 1259
Fnu4HI GCNGC 9 cut(s) 42, 45, 48, 339, 477, 805, 831, 870, 990
FokI GGATG 4 cut(s) 522, 744, 943, 947
Fsp4HI GCNGC 9 cut(s) 42, 45, 48, 339, 477, 805, 831, 870, 990
FspBI CTAG 1 cut(s) 918
GlaI GCGC 1 cut(s) 348
GluI GCNGC 9 cut(s) 42, 45, 48, 339, 477, 805, 831, 870, 990
GsaI CCCAGC 1 cut(s) 509
GsuI CTGGAG 2 cut(s) 199, 1304
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 1 cut(s) 341
HapII CCGG 1 cut(s) 729
HgaI GACGC 1 cut(s) 923
HhaI GCGC 1 cut(s) 349
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 5 cut(s) 374, 420, 601, 1193, 1229
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 2 cut(s) 738, 946
HindII GTYRAC 2 cut(s) 738, 946
HinfI GANTC 8 cut(s) 18, 125, 796, 815, 923, 1033, 1134, 1157
HpaI GTTAAC 1 cut(s) 738
HpaII CCGG 1 cut(s) 729
Hpy166II GTNNAC 5 cut(s) 558, 738, 946, 1048, 1260
Hpy188I TCNGA 8 cut(s) 76, 139, 175, 463, 879, 1063, 1241, 1267
Hpy188III TCNNGA 7 cut(s) 15, 216, 443, 538, 766, 1216, 1283
Hpy8I GTNNAC 5 cut(s) 558, 738, 946, 1048, 1260
HpyAV CCTTC 2 cut(s) 32, 789
HpyCH4III ACNGT 5 cut(s) 493, 1045, 1080, 1292, 1314
HpyCH4IV ACGT 3 cut(s) 405, 576, 740
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 190, 335, 344, 827
HpyF3I CTNAG 4 cut(s) 189, 429, 984, 1266
HpySE526I ACGT 3 cut(s) 405, 576, 740
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 5 cut(s) 374, 420, 601, 1193, 1229
HspAI GCGC 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 1231
KspAI GTTAAC 1 cut(s) 738
Kzo9I GATC 3 cut(s) 775, 1148, 1231
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 518, 688
Lsp1109I GCAGC 5 cut(s) 488, 791, 842, 881, 1001
LweI GCATC 3 cut(s) 302, 541, 925
MaeI CTAG 1 cut(s) 918
MaeII ACGT 3 cut(s) 405, 576, 740
MaeIII GTNAC 2 cut(s) 658, 979
MalI GATC 3 cut(s) 777, 1150, 1233
MbiI CCGCTC 1 cut(s) 305
MboI GATC 3 cut(s) 775, 1148, 1231
MboII GAAGA 7 cut(s) 63, 276, 288, 299, 650, 695, 1028
MflI RGATCY 1 cut(s) 1148
MluCI AATT 5 cut(s) 219, 252, 259, 271, 1106
MlyI GAGTC 4 cut(s) 119, 932, 1143, 1166
MmeI TCCRAC 3 cut(s) 99, 144, 735
MseI TTAA 4 cut(s) 243, 312, 737, 1321
MspA1I CMGCKG 2 cut(s) 50, 1177
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 1 cut(s) 729
MspR9I CCNGG 2 cut(s) 566, 624
MvaI CCWGG 2 cut(s) 566, 624
MwoI GCNNNNNNNGC 5 cut(s) 47, 190, 335, 344, 827
NcoI CCATGG 1 cut(s) 597
NdeII GATC 3 cut(s) 775, 1148, 1231
NlaIII CATG 5 cut(s) 374, 420, 601, 1193, 1229
NlaIV GGNNCC 3 cut(s) 7, 907, 966
NmuCI GTSAC 1 cut(s) 658
PasI CCCWGGG 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 312
PfeI GAWTC 4 cut(s) 18, 796, 815, 1033
PflFI GACNNNGTC 1 cut(s) 1133
PkrI GCNGC 9 cut(s) 43, 46, 49, 340, 478, 806, 832, 871, 991
PleI GAGTC 4 cut(s) 119, 931, 1142, 1165
PpsI GAGTC 4 cut(s) 119, 931, 1142, 1165
Psp6I CCWGG 2 cut(s) 564, 622
PspFI CCCAGC 1 cut(s) 505
PspGI CCWGG 2 cut(s) 564, 622
PspN4I GGNNCC 3 cut(s) 7, 907, 966
PstI CTGCAG 4 cut(s) 871, 979, 1129, 1147
PstNI CAGNNNCTG 3 cut(s) 836, 983, 1151
PsuI RGATCY 1 cut(s) 1148
PsyI GACNNNGTC 1 cut(s) 1133
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 4 cut(s) 243, 312, 737, 1321
SatI GCNGC 9 cut(s) 42, 45, 48, 339, 477, 805, 831, 870, 990
Sau3AI GATC 3 cut(s) 775, 1148, 1231
SbfI CCTGCAGG 1 cut(s) 1147
SchI GAGTC 4 cut(s) 119, 932, 1143, 1166
ScrFI CCNGG 2 cut(s) 566, 624
SdaI CCTGCAGG 1 cut(s) 1147
SfaNI GCATC 3 cut(s) 302, 541, 925
SfcI CTRYAG 6 cut(s) 867, 975, 1041, 1117, 1125, 1143
SmlI CTYRAG 2 cut(s) 311, 536
SmoI CTYRAG 2 cut(s) 311, 536
Sse8387I CCTGCAGG 1 cut(s) 1147
Sse9I AATT 5 cut(s) 219, 252, 259, 271, 1106
SspMI CTAG 1 cut(s) 918
StyD4I CCNGG 2 cut(s) 564, 622
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 5 cut(s) 493, 1045, 1080, 1292, 1314
TaiI ACGT 3 cut(s) 408, 579, 743
TaqI TCGA 2 cut(s) 636, 1217
TasI AATT 5 cut(s) 219, 252, 259, 271, 1106
TauI GCSGC 4 cut(s) 44, 47, 50, 341
TfiI GAWTC 4 cut(s) 18, 796, 815, 1033
Tru1I TTAA 4 cut(s) 243, 312, 737, 1321
Tru9I TTAA 4 cut(s) 243, 312, 737, 1321
TscAI CASTG 2 cut(s) 272, 1050
TseFI GTSAC 1 cut(s) 658
TseI GCWGC 5 cut(s) 476, 804, 830, 869, 989
Tsp45I GTSAC 1 cut(s) 658
TspDTI ATGAA 3 cut(s) 459, 701, 1153
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 2 cut(s) 272, 1050
Tth111I GACNNNGTC 1 cut(s) 1133
Vha464I CTTAAG 1 cut(s) 311
XapI RAATTY 1 cut(s) 219
XmiI GTMKAC 2 cut(s) 557, 1259
XspI CTAG 1 cut(s) 918
ZraI GACGTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.