Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003791

Physical Location & Seq

Genomic Coordinates Forward (+)

1280351 .. 1287904

Gene Structure 7554 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1020400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4407 bp

ATGTCCTGGGAGGACGAAGTCGTTATGCGTGATGTCACCCATGCGGGTATCGTCGTCGGCGACCGCATTGGCCGTGAAGTCGCGTCGCAGCTCGATCTTGAAGAAGCCCTAGAAGCGTCCCGATACGTTAGTCACCCCTATTCTACTCATCCCAGAGAGTGGCCACCTTTGGTTGAGGTGGTGGATACTTGGGAGTTGCCTCTCGTACTTATTGAAAGATACAATGCAGCTGGTGGGGAAGGAAATTCATTATGTGGGATTTTTCCCGAGATTCGTAGGGCATGGGCATCAGTAGATAACTCCTTGTTTCTCTGGCGTTTCGACAAGTGGGATGGACAATGTCCAGAATATAGCGGGGAGGATCAAGCTATTTGTGCTGTTGGCCTTGCTAAATCTAAACCTGGTGTTTTCATTGAAGCTATTCAGTATCTTTTGATTTTGGCAACCCCTGTTGAGTTAAGTCTTGTAGGTGTATGTTGCTCTGGAGGGGCTGGTGGCACAGATCCTTATGCAGAGGTCTCACTACAGCCATTGCCAGAGTATACAGTACCATCTGACGGAATCACAATGACATGTATCACGTGTACTGACAAGGGTCGTATTTTCCTTGCTGGGCGTGATGGCCACATTTATGAGCTACATTATACGACTGGATCAGGCTGGCAAAAGCGTTGTCGGAAAGTTTGTCTGACTGCTGGTATAGGAAGTGTTATTTCAAGATGGGTAGTACCTAATGTTTTTAAGTTTGGAGCTGTTGACCCCATTGTTGAAATGGTTTTTGATAATGAAAGGCATATCCTGTATGCACGGACTGAAGAAATGAAACTTCAGGTGTATATTGTGGGGCAAAATGCTGATGGGCCATTGAAGAAAGTGGCAGAAGAAAGGAATCTGATAAATCAGAGGGATCATTATGGGGGTAGACAATCAACTGGACCTAGAGGTCCAAATCGATTGCCAAAGTCATCAATAGTCTGCATATCACCATTGTCTACACTGGAATCCAAGTCTCTACACCTTGTTGCTGTTTTGTCAGATGGCAGAAGGATGTATCTTACCACTTCTCCTTCTAGTGGAAATCTAGGTGGGTTCAATACAAACCATTATAAACCTACCTGTTTAAAAGTTGTGACAACAAGGCCTTCTCCTCCACTAGGGGTCAGTGGTGGACTTGCCTTTGGAAGCGTGTCTCTTGCGGGTAGGTCTCAGAATGATGATCTCTCCCTAAAGGTTGAGGCAGGACATTATTCTGCTGGAACTCTTGTCCTTTCTGATTCATCTCCAGCAACCACAACTTCTCTTCTTATTGTGAGCCGTGATTCAACAGCACAATCAACTGGGTTAAGTACTTCAGGGATAAGTTCCAGGAGTTCTCGGGCACTGCGGGAATCTGTATCTTCTTTACCAGTTGAAGGTCGAATGCTTTTTGTAGCAGATGTTTTCCCCTTGCCTGATACTGCAACAACTGTACTGTCTCTATATTCAGAAATCGAATATGGTGGATATGAAGGCTTGGATGAGTCCTGTGAAAAGGTATCTGGAAAGCTTTGGGCCAGAGGTGACCTGTCAATCCAACATATATTGCCAAGAAGGCGCGTTGTTGTTTTCAGTACTATGGGAATGATGGAAATAGTTATCAATAGGCCTGTGGACATTCTGAGGAGGTTGTTTGAGGCCAACACCCCCAGGTCAATCTTAGAAGAATTCTTCACTCGTTTTGGAGCTGGTGAGGCTGCTGCAATGTGTCTAATGCTTGCAGCTAGGATAGTACATTCAGAAACTCTTATAAGCAATGTTGTTGCACAGAAGGCGGCTGAAGCTTTTGAAGACCCAAGATTAGTTGGAATGCCACAACTTGAGGGTAGCAGTGCATTATCTAATACTAGAACCGCTGCTGGGGGATTCAGCATGGGGCAAGTTGTTCAGGAGGCTGAGCCTGTGTTTTCAGGTGCACATGAAGGGCTCTGTTTGTGCGCTGCAAGGTTGCTATTTCCTTTGTGGGAACTTCCAGTCATGGTTATAAAAGGTGATTCTATGTCAGAAAATGGGTTAGTCGTATGCAGACTCTCTCTTGAGGCTATGCAAGTCCTTGAAAACAAGATTCGTTCGCTAGAGAGTTTGTTGAGGTCCAGAAGGAACCAAAGGAGGGGACTTTATGGCTGTGTTGCTGGTTTAGGAGATCTAACTGGTTCTATTCTTTATGGAGCCGGTTCAGAATTAGGTGGTGGTGACCGTGGTACGGTGAGGAACTTATTTGGTGCTTACTCAAGGAACACTGAGTCTAGTGATGGTGGTGTGTCTAATAAAAGGCAGAAATTACCATATAGTCCCGCTGAACTAGCAGCAATGGAGGTTAGGGCGATGGAATGCATCAGGCAGTTACTTCTTAGATCCAGTGAAGCCCTGTTTCTGCTCCAGCTTCTTTCACAGCACCATGTCACACGGCTGGTTCAGGGGCTTGATGCTACCGTACGACAGAAATTAGTTGTAATGACATTTCATCAGTTAGTTTGTTCGGAGGAAGGTGACCACCTTGCTACAAGGCTTATATCTGCTCTTATGGAGGTATATTATACTGGTCCTGATGGCAGGGGGGCAGTAGATGATATTAGTGGGAGATTACGGGAAGGCTGTCCAAGCTATTATAAGGAGTCTGATTACAAGTTTTTTCTAGCTGTGGAGTGTCTTGAGAGAGCTGCTATAGCTCCAGATCCTGACAAGGAGAGTCTTGCTAGAGAAGCCTTCAATTTCTTAAGTAAAGTTCCAGAATCTGCTGACTTACGAACTGTTTGCAAACGGTTTGAGGATTTGAGGTTTTATGAAGCAGTGGTTCGCTTGCCTTTACAAAAGGCACAGGCTCTTGATCCCGCAGGTGATGCTTTTAATGACCACATTGATGCAGCAGTTAGAGAGCATGCGCTTGCTCAACGTGAACAGTGCTATGAGATTGTCATCAGTGCATTGCGTTCTCTGAAAGGCGAGCCTTCTCAGAGGGAGTTTGGTTCTCCTTTGAGGCCTGCTGCACGATCTGTCCTTGATCAGGTCTCTCGGAACAAATATATTTCCCAGATTGTTCAGCTGGGCATTCAGTCTCCTGACCGATTATTTCACGAGTATTTGTACCGTGCTATGATTGATTTGGGCCTTGAAGATATATTGTTGGAGTATGGAGGTCCTGATTTGGTGCCGTTTCTAGAAAGTGCTGTTTCTGGGGTAACATCATCCAGTTCTCCAATTGGTCACTCAGGAACAAGCATCCCATCCAATCAAGCCAAATATTCTGAACTGTTGGCACGGTATTATGTCTCAAAGAGGCAGCATCTGCTTGCAGCTCGTGTATTACTAAGACTGGCAGAGAGGCGCTCAAGTGACACGGTGGATGTTCCTACTCTAGATCAAAGGTATCATTACCTGAGTAATGCAGTCCTACAGGCAAAAAATGCAAGTAACAATGATGGCATGGTAGGTTCTGCTCGAGGCGCTTATGATGATGGGTTGTTAGATTTGCTGGAAGGAAAGCTTTCTGTCCTTCGTTTTCAGATAAAGATCAAGGAGGAACTTGAGGCTTTTGCCTCCAGGATAGAAGCTTTGGCTGATGCATCGGAGCCTGTCCAAAGTGGAACTTCTGGTGATGCCAATCTTGCAATTATTGCACGAGAAAAGGCCAAGGAGCTATCATTAGATTTAAAGAATATAACTCAGCTCTACAATGAATATGCTCTTCCATTTGAACTTTGGGAGATATGTCTAGAGATGTTATACTTCGCATACTATTCTGGTGATGCTGATAGCAGCATCGTACGAGAGACGTGGGCTAGATTGATTGATCAAGCTCTTTCAAGGGGTGGCATTGCTGAAGCTTGTGCAGTATTGAAGAGAGTTGGTTCTCACATTTATCCTGGAGATGGAGCTGGTTTACCCTTAGACACATTGTGTCTCCACCTCGAGAAAGCTGCAATGGATCGACTGGAATCAGGGGTTGAATCGGTTGGAGATGAAGATGTGGCAAGGGCTCTTCTTGCTGCATGCAAGGGTGCAACCGAGCCTGTACTGAACACTTATGACCAGTTGTTGACAAGTGGTGCTATTTTGCCATCACCAAACCTTAGGTTACGCCTTCTCCGATCAGTGCTGGTAGTACTTCGAGAGTGGGCAATGTCTGTGTTTGCACAGAGAATGGGTACTAGTGCTACTGGAGCTTCGTTAATTTTAGGTGGAACTTTCTCGATGGAGCAAACGGCAGTCCTCAACCAAGGGGTTCGAGATAAGATCTCAAGTGCAGCAAACAGGTACATGACCGAGGTACGAAGGTTGGCCCTTCCACAAAGCCAGACTGAAGCTGTTCACCATGGCTTTCGAGAACTTGAAGAGTCGCTGACCAGTCCCTTTTCTTTTGATCGTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000972 GO:0003674 GO:0005198 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005886 GO:0005911 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006998 GO:0008104 GO:0008150 GO:0008152 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010256 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016032 GO:0016043 GO:0017038 GO:0017056 GO:0030054 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0036228 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044611 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051236 GO:0051276 GO:0051641 GO:0051649 GO:0051704 GO:0055044 GO:0061024 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071944 GO:0072594 GO:0072657 GO:0090435

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nucleoporin_C Nucleoporin_N

Protein Analysis

1469

Amino Acids

160.67

Weight (kDa)

5.6

Isoelectric Point (pI)

48.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nucleoporin_N	PF08801	54 - 542	3.2e-73	Nup133 N terminal like
Nucleoporin_C	PF03177	783 - 1385	2e-66	Non-repetitive/WGA-negative nucleoporin C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14850
fragaria_vesca	FvH4_4g33560 FvH4_4g33560 FvH4_4g33560
malus_domestica	MD13G1020400.v1.1 MD16G1018600.v1.1 MD16G1018800.v1.1
prunus_persica	Prupe.1G333300_v2.0.a1
pyrus_communis	pycom13g01720 pycom16g01690
rosa_chinensis	RchiOBHm_Chr2g0167011 RchiOBHm_Chr2g0167021 RchiOBHm_Chr4g0443061
rosa_laevigata	RLG00000005957 RLG00000021689
rosa_multiflora	Rmu_co7980658.1_g000001 Rmu_sc0007968.1_g000017 Rmu_sc0008069.1_g000005 Rmu_sc0010966.1_g000010 Rmu_sc0039405.1_g000001 Rmu_sc0039405.1_g000002
rosa_roxburghii	Rroxscaffold_2G00083800 Rroxscaffold_5G00383690
rosa_rugosa	Rorug04G0344100
rosa_samantha	Rh2AG594500 Rh2AG594600 Rh2BG605800 Rh2BG605900 Rh2CG576700 Rh2DG617400 Rh4BG409900 Rh4DG404800
rosa_wichuraiana	Rw2G049490 Rw4G034330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1107, 1787, 2021, 2646
AarI	CACCTGC	1 cut(s)	2861
AasI	GACNNNNNNGTC	1 cut(s)	942
Acc36I	ACCTGC	1 cut(s)	2861
AccB1I	GGYRCC	1 cut(s)	3184
AccB7I	CCANNNNNTGG	1 cut(s)	159
AccI	GTMKAC	3 cut(s)	542, 922, 992
AccII	CGCG	2 cut(s)	83, 1596
AciI	CCGC	9 cut(s)	44, 64, 354, 1196, 1384, 1811, 1890, 2331, 2868
AclWI	GGATC	8 cut(s)	369, 497, 661, 915, 2385, 2705, 2858, 3969
AcoI	YGGCCR	3 cut(s)	70, 161, 622
AcsI	RAATTY	2 cut(s)	244, 1703
AcuI	CTGAAG	6 cut(s)	812, 834, 1335, 1836, 3876, 4356
AcvI	CACGTG	1 cut(s)	582
AdeI	CACNNNGTG	1 cut(s)	3933
AflII	CTTAAG	1 cut(s)	2752
AflIII	ACRYGT	2 cut(s)	572, 581
AhlI	ACTAGT	1 cut(s)	4184
AjiI	CACGTC	1 cut(s)	3810
AjnI	CCWGG	6 cut(s)	5, 400, 1364, 1685, 3575, 3898
Alw21I	GWGCWC	1 cut(s)	1954
Alw44I	GTGCAC	1 cut(s)	1950
AlwI	GGATC	8 cut(s)	369, 497, 661, 915, 2385, 2705, 2858, 3969
AlwNI	CAGNNNCTG	3 cut(s)	2714, 2771, 3322
Ama87I	CYCGRG	4 cut(s)	266, 1374, 3474, 3944
ApaLI	GTGCAC	1 cut(s)	1950
ApoI	RAATTY	2 cut(s)	244, 1703
ArsI	GACNNNNNNTTYG	2 cut(s)	658, 690
Asp700I	GAANNNNTTC	3 cut(s)	420, 3520, 4341
AspLEI	GCGC	5 cut(s)	1596, 1976, 2920, 3363, 3482
AspS9I	GGNCC	9 cut(s)	860, 935, 944, 1551, 2127, 2579, 3142, 3173, 4315
AvaI	CYCGRG	4 cut(s)	266, 1374, 3474, 3944
AvaII	GGWCC	5 cut(s)	935, 944, 2127, 2579, 3173
AxyI	CCTNAGG	1 cut(s)	4106
BaeGI	GKGCMC	2 cut(s)	1381, 1954
BaeI	ACNNNNGTAYC	4 cut(s)	115, 148, 4295, 4328
BalI	TGGCCA	2 cut(s)	163, 624
BanI	GGYRCC	1 cut(s)	3184
BanII	GRGCYC	2 cut(s)	1965, 4015
BauI	CACGAG	3 cut(s)	3110, 3333, 3654
BbrPI	CACGTG	1 cut(s)	582
BbsI	GAAGAC	1 cut(s)	1833
Bbv12I	GWGCWC	1 cut(s)	1954
BceAI	ACGGC	5 cut(s)	57, 1299, 2459, 3172, 4254
BcgI	CGANNNNNNTGC	4 cut(s)	2772, 2806, 3935, 3969
BciT130I	CCWGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
BciVI	GTATCC	1 cut(s)	178
BclI	TGATCA	2 cut(s)	3037, 3826
BcuI	ACTAGT	1 cut(s)	4184
BfmI	CTRYAG	3 cut(s)	524, 2700, 3428
BfoI	RGCGCY	2 cut(s)	3364, 3483
BfrI	CTTAAG	1 cut(s)	2752
BfuAI	ACCTGC	1 cut(s)	2861
BfuI	GTATCC	1 cut(s)	178
BglI	GCCNNNNNGGC	1 cut(s)	1591
BglII	AGATCT	2 cut(s)	2179, 4269
BlpI	GCTNAGC	1 cut(s)	1932
BmcAI	AGTACT	3 cut(s)	1348, 1612, 4140
Bme1390I	CCNGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
Bme18I	GGWCC	5 cut(s)	935, 944, 2127, 2579, 3173
BmeT110I	CYCGRG	4 cut(s)	266, 1374, 3474, 3944
BmgBI	CACGTC	1 cut(s)	3810
BmgT120I	GGNCC	9 cut(s)	860, 935, 944, 1551, 2127, 2579, 3142, 3173, 4315
BmiI	GGNNCC	4 cut(s)	2138, 2206, 3186, 3606
BmrFI	CCNGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
BmrI	ACTGGG	1 cut(s)	1347
BmuI	ACTGGG	1 cut(s)	1347
BoxI	GACNNNNGTC	1 cut(s)	594
BpiI	GAAGAC	1 cut(s)	1833
BplI	GAGNNNNNCTC	2 cut(s)	3347, 3379
BpmI	CTGGAG	7 cut(s)	504, 1266, 2399, 2691, 3559, 3921, 4215
Bpu1102I	GCTNAGC	1 cut(s)	1932
BpuEI	CTTGAG	7 cut(s)	1877, 2093, 2251, 2708, 3349, 3581, 4258
Bsa29I	ATCGAT	1 cut(s)	952
BsaAI	YACGTR	1 cut(s)	582
BsaBI	GATNNNNATC	3 cut(s)	2951, 3545, 3636
BsaI	GGTCTC	3 cut(s)	523, 1209, 3049
BsaJI	CCNNGG	7 cut(s)	6, 1685, 2233, 3666, 4252, 4299, 4348
BsaXI	ACNNNNNCTCC	4 cut(s)	1048, 1078, 4104, 4134
Bse118I	RCCGGY	1 cut(s)	2207
Bse21I	CCTNAGG	1 cut(s)	4106
Bse3DI	GCAATG	8 cut(s)	530, 1746, 1798, 2352, 2960, 3849, 3963, 4161
Bse8I	GATNNNNATC	3 cut(s)	2951, 3545, 3636
BseBI	CCWGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
BseCI	ATCGAT	1 cut(s)	952
BseDI	CCNNGG	7 cut(s)	6, 1685, 2233, 3666, 4252, 4299, 4348
BseGI	GGATG	8 cut(s)	148, 337, 1053, 1522, 3221, 3255, 3260, 3385
BseJI	GATNNNNATC	3 cut(s)	2951, 3545, 3636
BseMI	GCAATG	8 cut(s)	530, 1746, 1798, 2352, 2960, 3849, 3963, 4161
BseMII	CTCAG	8 cut(s)	1220, 1649, 1923, 2268, 3002, 3258, 3404, 3713
BseRI	GAGGAG	2 cut(s)	1137, 1675
BseSI	GKGCMC	2 cut(s)	1381, 1954
BseYI	CCCAGC	3 cut(s)	611, 1895, 3079
BsgI	GTGCAG	3 cut(s)	3006, 3885, 4299
Bsh1236I	CGCG	2 cut(s)	83, 1596
Bsh1285I	CGRYCG	1 cut(s)	64
BshNI	GGYRCC	1 cut(s)	3184
BshVI	ATCGAT	1 cut(s)	952
BsiEI	CGRYCG	1 cut(s)	64
BsiHKAI	GWGCWC	1 cut(s)	1954
BsiHKCI	CYCGRG	4 cut(s)	266, 1374, 3474, 3944
BsiSI	CCGG	1 cut(s)	2208
BsiWI	CGTACG	2 cut(s)	2470, 3799
BslFI	GGGAC	4 cut(s)	103, 2163, 2313, 4368
BsmBI	CGTCTC	1 cut(s)	3800
BsmFI	GGGAC	4 cut(s)	103, 2163, 2313, 4368
BsmI	GAATGC	4 cut(s)	1425, 1851, 2372, 3084
Bso31I	GGTCTC	3 cut(s)	523, 1209, 3049
BsoBI	CYCGRG	4 cut(s)	266, 1374, 3474, 3944
Bsp1286I	GDGCHC	4 cut(s)	1381, 1954, 1965, 4015
Bsp1720I	GCTNAGC	1 cut(s)	1932
Bsp19I	CCATGG	1 cut(s)	4348
BspACI	CCGC	9 cut(s)	44, 64, 354, 1196, 1384, 1811, 1890, 2331, 2868
BspCNI	CTCAG	8 cut(s)	1219, 1650, 1924, 2269, 3001, 3257, 3405, 3712
BspDI	ATCGAT	1 cut(s)	952
BspFNI	CGCG	2 cut(s)	83, 1596
BspLI	GGNNCC	4 cut(s)	2138, 2206, 3186, 3606
BspMI	ACCTGC	1 cut(s)	2861
BspPI	GGATC	8 cut(s)	369, 497, 661, 915, 2385, 2705, 2858, 3969
BspQI	GCTCTTC	2 cut(s)	3726, 4020
BspT107I	GGYRCC	1 cut(s)	3184
BspTI	CTTAAG	1 cut(s)	2752
BspTNI	GGTCTC	3 cut(s)	523, 1209, 3049
BsrDI	GCAATG	8 cut(s)	530, 1746, 1798, 2352, 2960, 3849, 3963, 4161
BsrFI	RCCGGY	1 cut(s)	2207
BssAI	RCCGGY	1 cut(s)	2207
BssECI	CCNNGG	7 cut(s)	6, 1685, 2233, 3666, 4252, 4299, 4348
BssNAI	GTATAC	1 cut(s)	543
BssSI	CACGAG	3 cut(s)	3110, 3333, 3654
BssT1I	CCWWGG	3 cut(s)	3666, 4252, 4348
Bst1107I	GTATAC	1 cut(s)	543
Bst2BI	CACGAG	3 cut(s)	3110, 3333, 3654
Bst2UI	CCWGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
Bst6I	CTCTTC	5 cut(s)	1305, 3726, 3869, 4020, 4362
BstAFI	CTTAAG	1 cut(s)	2752
BstAPI	GCANNNNNTGC	4 cut(s)	2876, 3322, 3440, 3650
BstBAI	YACGTR	1 cut(s)	582
BstC8I	GCNNGC	9 cut(s)	662, 1755, 2837, 2916, 2922, 2981, 3018, 3327, 4027
BstDSI	CCRYGG	2 cut(s)	2233, 4348
BstEII	GGTNACC	3 cut(s)	1559, 2228, 2525
BstENI	CCTNNNNNAGG	2 cut(s)	1152, 3038
BstF5I	GGATG	8 cut(s)	148, 337, 1053, 1522, 3221, 3255, 3260, 3385
BstFNI	CGCG	2 cut(s)	83, 1596
BstH2I	RGCGCY	2 cut(s)	3364, 3483
BstHHI	GCGC	5 cut(s)	1596, 1976, 2920, 3363, 3482
BstMCI	CGRYCG	1 cut(s)	64
BstNI	CCWGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
BstNSI	RCATGY	3 cut(s)	576, 2918, 4029
BstPAI	GACNNNNGTC	1 cut(s)	594
BstPI	GGTNACC	3 cut(s)	1559, 2228, 2525
BstSCI	CCNGG	6 cut(s)	5, 400, 1364, 1685, 3575, 3898
BstSFI	CTRYAG	3 cut(s)	524, 2700, 3428
BstSLI	GKGCMC	2 cut(s)	1381, 1954
BstUI	CGCG	2 cut(s)	83, 1596
BstV2I	GAAGAC	1 cut(s)	1833
BstX2I	RGATCY	5 cut(s)	502, 2179, 2390, 2710, 4269
BstYI	RGATCY	5 cut(s)	502, 2179, 2390, 2710, 4269
BstZ17I	GTATAC	1 cut(s)	543
Bsu15I	ATCGAT	1 cut(s)	952
Bsu36I	CCTNAGG	1 cut(s)	4106
BsuI	GTATCC	1 cut(s)	178
BsuTUI	ATCGAT	1 cut(s)	952
BtgI	CCRYGG	2 cut(s)	2233, 4348
BtgZI	GCGATG	1 cut(s)	2375
BtrI	CACGTC	1 cut(s)	3810
BtsCI	GGATG	8 cut(s)	148, 337, 1053, 1522, 3221, 3255, 3260, 3385
BtsI	GCAGTG	3 cut(s)	1379, 1873, 2832
BveI	ACCTGC	1 cut(s)	2861
Cac8I	GCNNGC	9 cut(s)	662, 1755, 2837, 2916, 2922, 2981, 3018, 3327, 4027
CaiI	CAGNNNCTG	3 cut(s)	2714, 2771, 3322
CfoI	GCGC	5 cut(s)	1596, 1976, 2920, 3363, 3482
Cfr10I	RCCGGY	1 cut(s)	2207
Cfr13I	GGNCC	9 cut(s)	860, 935, 944, 1551, 2127, 2579, 3142, 3173, 4315
ClaI	ATCGAT	1 cut(s)	952
CseI	GACGC	2 cut(s)	72, 105
CsiI	ACCWGGT	1 cut(s)	400
DraI	TTTAAA	2 cut(s)	1122, 3687
DraIII	CACNNNGTG	1 cut(s)	3933
DrdI	GACNNNNNNGTC	1 cut(s)	942
DseDI	GACNNNNNNGTC	1 cut(s)	942
EaeI	YGGCCR	3 cut(s)	70, 161, 622
Eam1104I	CTCTTC	5 cut(s)	1305, 3726, 3869, 4020, 4362
EarI	CTCTTC	5 cut(s)	1305, 3726, 3869, 4020, 4362
Eco130I	CCWWGG	3 cut(s)	3666, 4252, 4348
Eco147I	AGGCCT	3 cut(s)	1141, 1645, 3016
Eco24I	GRGCYC	2 cut(s)	1965, 4015
Eco31I	GGTCTC	3 cut(s)	523, 1209, 3049
Eco47I	GGWCC	5 cut(s)	935, 944, 2127, 2579, 3173
Eco57I	CTGAAG	6 cut(s)	812, 834, 1335, 1836, 3876, 4356
Eco72I	CACGTG	1 cut(s)	582
Eco81I	CCTNAGG	1 cut(s)	4106
Eco88I	CYCGRG	4 cut(s)	266, 1374, 3474, 3944
Eco91I	GGTNACC	3 cut(s)	1559, 2228, 2525
EcoNI	CCTNNNNNAGG	2 cut(s)	1152, 3038
EcoO109I	RGGNCCY	1 cut(s)	3173
EcoO65I	GGTNACC	3 cut(s)	1559, 2228, 2525
EcoRI	GAATTC	1 cut(s)	1703
EcoRII	CCWGG	6 cut(s)	5, 400, 1364, 1685, 3575, 3898
EcoT14I	CCWWGG	3 cut(s)	3666, 4252, 4348
EcoT22I	ATGCAT	2 cut(s)	2372, 3601
EcoT38I	GRGCYC	2 cut(s)	1965, 4015
ErhI	CCWWGG	3 cut(s)	3666, 4252, 4348
Esp3I	CGTCTC	1 cut(s)	3800
FalI	AAGNNNNNCTT	4 cut(s)	3504, 3536, 3607, 3639
FaqI	GGGAC	4 cut(s)	103, 2163, 2313, 4368
FauI	CCCGC	6 cut(s)	37, 347, 1189, 1377, 2338, 2875
FbaI	TGATCA	2 cut(s)	3037, 3826
FblI	GTMKAC	3 cut(s)	542, 922, 992
FokI	GGATG	8 cut(s)	135, 344, 1060, 1529, 3208, 3242, 3247, 3392
FriOI	GRGCYC	2 cut(s)	1965, 4015
GlaI	GCGC	5 cut(s)	1595, 1975, 2919, 3362, 3481
GsaI	CCCAGC	3 cut(s)	615, 1899, 3083
GsuI	CTGGAG	7 cut(s)	504, 1266, 2399, 2691, 3559, 3921, 4215
HaeII	RGCGCY	2 cut(s)	3364, 3483
HapII	CCGG	1 cut(s)	2208
HgaI	GACGC	2 cut(s)	72, 105
HhaI	GCGC	5 cut(s)	1596, 1976, 2920, 3363, 3482
Hin6I	GCGC	5 cut(s)	1594, 1974, 2918, 3361, 3480
HinP1I	GCGC	5 cut(s)	1594, 1974, 2918, 3361, 3480
HincII	GTYRAC	2 cut(s)	757, 4074
HindII	GTYRAC	2 cut(s)	757, 4074
HindIII	AAGCTT	5 cut(s)	1544, 1818, 3518, 3585, 3858
HpaII	CCGG	1 cut(s)	2208
Hpy99I	CGWCG	3 cut(s)	56, 59, 88
HpyCH4IV	ACGT	4 cut(s)	126, 581, 2929, 3809
HpySE526I	ACGT	4 cut(s)	126, 581, 2929, 3809
HspAI	GCGC	5 cut(s)	1594, 1974, 2918, 3361, 3480
Ksp22I	TGATCA	2 cut(s)	3037, 3826
LguI	GCTCTTC	2 cut(s)	3726, 4020
MabI	ACCWGGT	1 cut(s)	400
MaeII	ACGT	4 cut(s)	126, 581, 2929, 3809
MfeI	CAATTG	1 cut(s)	3234
MflI	RGATCY	5 cut(s)	502, 2179, 2390, 2710, 4269
MhlI	GDGCHC	4 cut(s)	1381, 1954, 1965, 4015
MlsI	TGGCCA	2 cut(s)	163, 624
MluCI	AATT	9 cut(s)	244, 1703, 2216, 2315, 2480, 2746, 3234, 3645, 4206
MluNI	TGGCCA	2 cut(s)	163, 624
MlyI	GAGTC	6 cut(s)	1529, 2058, 2288, 2660, 2734, 4379
MmeI	TCCRAC	5 cut(s)	656, 1597, 1822, 3141, 3970
Mox20I	TGGCCA	2 cut(s)	163, 624
Mph1103I	ATGCAT	2 cut(s)	2372, 3601
MroXI	GAANNNNTTC	3 cut(s)	420, 3520, 4341
MscI	TGGCCA	2 cut(s)	163, 624
MseI	TTAA	8 cut(s)	458, 741, 1121, 1343, 2753, 2883, 3686, 4205
MslI	CAYNNNNRTG	2 cut(s)	630, 2895
Msp20I	TGGCCA	2 cut(s)	163, 624
MspA1I	CMGCKG	4 cut(s)	230, 1892, 2333, 3079
MspCI	CTTAAG	1 cut(s)	2752
MspI	CCGG	1 cut(s)	2208
MspR9I	CCNGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
MunI	CAATTG	1 cut(s)	3234
Mva1269I	GAATGC	4 cut(s)	1425, 1851, 2372, 3084
MvaI	CCWGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
MvnI	CGCG	2 cut(s)	83, 1596
NcoI	CCATGG	1 cut(s)	4348
NlaIV	GGNNCC	4 cut(s)	2138, 2206, 3186, 3606
NmuCI	GTSAC	9 cut(s)	34, 131, 1129, 1559, 2228, 2437, 2525, 3239, 3368
NsiI	ATGCAT	2 cut(s)	2372, 3601
NspI	RCATGY	3 cut(s)	576, 2918, 4029
PaeI	GCATGC	2 cut(s)	2918, 4029
PaeR7I	CTCGAG	2 cut(s)	3474, 3944
PaqCI	CACCTGC	1 cut(s)	2861
PceI	AGGCCT	3 cut(s)	1141, 1645, 3016
PciI	ACATGT	1 cut(s)	572
PciSI	GCTCTTC	2 cut(s)	3726, 4020
PcsI	WCGNNNNNNNCGW	2 cut(s)	57, 4120
PctI	GAATGC	4 cut(s)	1425, 1851, 2372, 3084
PdmI	GAANNNNTTC	3 cut(s)	420, 3520, 4341
Pfl23II	CGTACG	2 cut(s)	2470, 3799
PflFI	GACNNNGTC	2 cut(s)	17, 339
PflMI	CCANNNNNTGG	1 cut(s)	159
PfoI	TCCNGGA	3 cut(s)	1364, 3575, 3898
PleI	GAGTC	6 cut(s)	1528, 2058, 2287, 2659, 2733, 4378
PmaCI	CACGTG	1 cut(s)	582
PmlI	CACGTG	1 cut(s)	582
PpsI	GAGTC	6 cut(s)	1528, 2058, 2287, 2659, 2733, 4378
Ppu21I	YACGTR	1 cut(s)	582
PpuMI	RGGWCCY	1 cut(s)	3173
PscI	ACATGT	1 cut(s)	572
PshAI	GACNNNNGTC	1 cut(s)	594
PsiI	TTATAA	4 cut(s)	1107, 1787, 2021, 2646
Psp5II	RGGWCCY	1 cut(s)	3173
Psp6I	CCWGG	6 cut(s)	5, 400, 1364, 1685, 3575, 3898
PspCI	CACGTG	1 cut(s)	582
PspEI	GGTNACC	3 cut(s)	1559, 2228, 2525
PspFI	CCCAGC	3 cut(s)	611, 1895, 3079
PspGI	CCWGG	6 cut(s)	5, 400, 1364, 1685, 3575, 3898
PspLI	CGTACG	2 cut(s)	2470, 3799
PspN4I	GGNNCC	4 cut(s)	2138, 2206, 3186, 3606
PspPI	GGNCC	9 cut(s)	860, 935, 944, 1551, 2127, 2579, 3142, 3173, 4315
PspPPI	RGGWCCY	1 cut(s)	3173
PspXI	VCTCGAGB	1 cut(s)	3474
PstNI	CAGNNNCTG	3 cut(s)	2714, 2771, 3322
PsuI	RGATCY	5 cut(s)	502, 2179, 2390, 2710, 4269
PsyI	GACNNNGTC	2 cut(s)	17, 339
PvuII	CAGCTG	2 cut(s)	230, 3079
RseI	CAYNNNNRTG	2 cut(s)	630, 2895
SapI	GCTCTTC	2 cut(s)	3726, 4020
SaqAI	TTAA	8 cut(s)	458, 741, 1121, 1343, 2753, 2883, 3686, 4205
Sau96I	GGNCC	9 cut(s)	860, 935, 944, 1551, 2127, 2579, 3142, 3173, 4315
ScaI	AGTACT	3 cut(s)	1348, 1612, 4140
SchI	GAGTC	6 cut(s)	1529, 2058, 2288, 2660, 2734, 4379
ScrFI	CCNGG	6 cut(s)	7, 402, 1366, 1687, 3577, 3900
SduI	GDGCHC	4 cut(s)	1381, 1954, 1965, 4015
SexAI	ACCWGGT	1 cut(s)	400
SfcI	CTRYAG	3 cut(s)	524, 2700, 3428
Sfr274I	CTCGAG	2 cut(s)	3474, 3944
SinI	GGWCC	5 cut(s)	935, 944, 2127, 2579, 3173
SlaI	CTCGAG	2 cut(s)	3474, 3944
SmiMI	CAYNNNNRTG	2 cut(s)	630, 2895
SpeI	ACTAGT	1 cut(s)	4184
SphI	GCATGC	2 cut(s)	2918, 4029
Sse9I	AATT	9 cut(s)	244, 1703, 2216, 2315, 2480, 2746, 3234, 3645, 4206
SseBI	AGGCCT	3 cut(s)	1141, 1645, 3016
SsiI	CCGC	9 cut(s)	44, 64, 354, 1196, 1384, 1811, 1890, 2331, 2868
SspI	AATATT	1 cut(s)	3278
StuI	AGGCCT	3 cut(s)	1141, 1645, 3016
StyD4I	CCNGG	6 cut(s)	5, 400, 1364, 1685, 3575, 3898
StyI	CCWWGG	3 cut(s)	3666, 4252, 4348
TaiI	ACGT	4 cut(s)	129, 584, 2932, 3812
TaqII	GACCGA	2 cut(s)	3114, 4313
TasI	AATT	9 cut(s)	244, 1703, 2216, 2315, 2480, 2746, 3234, 3645, 4206
TatI	WGTACW	7 cut(s)	584, 1346, 1468, 1610, 1768, 4048, 4138
TauI	GCSGC	1 cut(s)	1814
Tru1I	TTAA	8 cut(s)	458, 741, 1121, 1343, 2753, 2883, 3686, 4205
Tru9I	TTAA	8 cut(s)	458, 741, 1121, 1343, 2753, 2883, 3686, 4205
TseFI	GTSAC	9 cut(s)	34, 131, 1129, 1559, 2228, 2437, 2525, 3239, 3368
Tsp45I	GTSAC	9 cut(s)	34, 131, 1129, 1559, 2228, 2437, 2525, 3239, 3368
TspGWI	ACGGA	2 cut(s)	573, 823
Tth111I	GACNNNGTC	2 cut(s)	17, 339
Van91I	CCANNNNNTGG	1 cut(s)	159
Vha464I	CTTAAG	1 cut(s)	2752
VneI	GTGCAC	1 cut(s)	1950
VpaK11BI	GGWCC	5 cut(s)	935, 944, 2127, 2579, 3173
XagI	CCTNNNNNAGG	2 cut(s)	1152, 3038
XapI	RAATTY	2 cut(s)	244, 1703
XbaI	TCTAGA	3 cut(s)	3193, 3391, 3748
XceI	RCATGY	3 cut(s)	576, 2918, 4029
XcmI	CCANNNNNNNNNTGG	2 cut(s)	769, 3732
XhoI	CTCGAG	2 cut(s)	3474, 3944
XmiI	GTMKAC	3 cut(s)	542, 922, 992
XmnI	GAANNNNTTC	3 cut(s)	420, 3520, 4341
ZrmI	AGTACT	3 cut(s)	1348, 1612, 4140
Zsp2I	ATGCAT	2 cut(s)	2372, 3601

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.