Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003791

Physical Location & Seq

Genomic Coordinates Forward (+)

77067853 .. 77077813

Gene Structure 9961 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G049490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3264 bp

ATGTGGTCGTCAGAGGATGAGAACAAAATCGTAATGAGCGATGTCACCAACGCCGGCTTGGTTGTCAGCCACCACATTGGCCTGGAATTGCTTCCACCTCTGGCTGAACTGGTGGGTTCTTGGGATTTGGCGCCTCAGAAGCTTCTTGAAAGATACAATGAAAATGGTGGTGAGGAAAGAGAAGGCACCACTTTGTGTGGAATATTCCCACAAATTCATAGGGCATGGGCTTCAACAGACAGCTCCTTGTTTGACCCTCCTAACACCTTGTTTCTTTGGGGGTTCGATGGCTACTCTTTTCAAGAATACAAGGGAAATGGTGAAGCAACTATTTGTGCCGTTGGACTTTGCAAACCGAAAGCTGGAGTTTTCAACGAAGCTATTCGGTATGTTTTGGTATTACCAATGCCTGATGAGTTAATTCTGGTAGGAGTGAGTTTACCGTCCGATGGGGTCAGCATGACTTGTGTTACATGTAGTGACAATGGTCGGATATTCATGGCGGGGAGGGATGGACACATTTATGAGCTGAAGTATACAAAGTGGCAATGCGGTGTTGGAAGGTTTGTATGGGTAGTGCCGGCACATGTTTTTAAGTTTGGGGTTGTTGACCCAGTTGTAGATATGGTTTTCGATAATGAGAGAAACATCTTGTATGCAAGAACAGCAGCAATGAAGATTCAGGTTTATGATGCTGATGGCGGGGGATTGAAGAAAACATCAATAGTTTGCATGGCACCATTGTCTACCATGGAGTCTAAGTCTCTACACCTTGTTGCTGTTTTGTCAGATGGAAGAAGAATGTACTTTACTACTTCCTCTGGATTTAGTAAAAAACATAATAGACCTAGTTGTTTGAGAGTCGTGACAACTAGGCCTGCTCCTACAGACATAGTTAATGCAGAGCATTTTTACTCTGCTGGAACTCTAATTCTTTCGGATTCATCAACTAGCTCAGGGCCAAGCATTTTGGGGACAAGTACAAGGAGTTGTTCTCGTAAATTATGGGAACATGTATATTCTTTGCCAGTTAAAGGAGAGATTCTTTCTGTAGCAGATAAATTACCTCAGCTTGATACTGCAAGGACTCTATGTTATGGTGGAGGCGAGCTTTCTAATCAAATTGTATTGCCAAGGAAGCAACTTGTGTTCTCGATCAATAGGCCTGTGGATATTCTGAGGAGGTTGTTGGCGTTCTACTCCCCCAGGGCAATGTTACAAGACTTCTTCAATCACTACGGAGCTGGTGAGGCGGCTGCGATGTGTTTAATGCTAGCAGCTGGGATAGTCCATCCAGTAGTTCATATCAGCAAAGTTGCGGAGATTGTTGAGGAGGCTGAGCCTGATTTTTCAGCTGTACATGAAGGGCTATGTTTGTGCTCGGCAAGGCTGCTTTTACCTCTATGGGAACTTCCTTTCGTGGTGGAAGAAGGCGGTTTAGTCATGTGCAGACTATCTCTTGAGGCAATGCAGAATTTTCTGTGGTTGAGAAGGAAGCAAAGGAGGGGCCTTTATGGCCGTGTTACTGGTCTTGGGTACTTGACTGGTTATAGAGATGAATCAAAATATGATGGCAGCTATGAAGCTCAAGTCCTGCTCCAACGTCTTCCGCAGCAAGGTCTAATCACTTGCTTGGTATTAGTCCAATTGACTTTTCATCAGTTAGTTTGTTCTGAGGAGGGTAACCACCTTGCTACAAGGCTCATATCTGGTCTGACAAAGTATTATACTGATCAAGGCAAGGGGACAGTACATAAAATTAGTGCAGTACTGCAGGAAGGTTGTCCAAGCTATTACAAGGAGTCTGATGGCAAGTATTATCTAGCTATGGATTGTCTTCAGAGAGCTGTTTTAAATAAAGATGCTGAGGAGATGGAGAAACTTGCCAGAGAAGCCTACAGTTGTAAAGTTCCAAAATCTGCTAATTTACAGGTGGTTTGCAAATGGTTTGAGGGTTTGAGGTTTTATGAAGCAGTGGTCCACTTACCTATACAGAAGGCACAAGCTCTTGACCCCAAAGGTGATGCTTTTAATAACCAAATTGATGCAGCAGTTCGAGAAGATGCAGTTGCTAAACGTGTTCAGTGTTATGAGATTGTCATTAGTGCACTGCGTTCTCTAAAAGGTGTATATGTTGGTTCCTCTTCTCGGAACAGACATGTGTGCCAAATTGTTCAGCTTGGCATCCGATTTCCAGACAGGTTATTTCATGAGTACTTGTGCCGTGCTATGATTGATGTCGGCCTTGAAAATGAGCTGTTTGAGTTTGGAGATCCTAATTTGGTGCCTTTACTACAAAGTGTAGCCGGTGAACCTATACAAGAGGTTCAAGCTATTTCTCCCATTGGTCATTCAGGAACACTTTTTCCAACTAAAAAAGCCAAATATTGTGAGGTTTTGGCAAGTTACTATGCCTTGAAAGGGCAGCATCGGCTTGCAGCTCATGCATTGTGTCAATACTTAACTAGGGCACTCCTACAAGAAAAAGTTGCAAGTAACAGTACAGACTTAGTACAGCTGTTGGAAGGAACGCTTGGTGTTCTCCATTTTCAGATAAAGATCAAAGCGGAACTTGAGGCTTTACCTAGTGCATCTGAGTCTGTCAAAAAGGCCAAAGAGGTATCACTAGACTTAAAGAGTCTAAGTGAGCTATACAATGACTATGCTGTTCCATTTCAACTTTGGGAGATATCTCTAGAAATTAGCAGTATAAGAGAAGCTTGGACTAAAGTAGTTGATGAAGCACTTTCAAAAAGTGGCGTCGCTGAAGCTTGTTGGGTATTGAAGAGATTTGGTTCTTACACACTGTGCCTCCACCTTGAGAAAGCTGCATTGGAAAGACTGGAATCAGGGGCTGAATCCATCGGAGATGAAGATGTGGCAAGGGCTCTTCTTGCTGCTTCTTCCAAGGGTGCAACTGAGCCTGCATTGTTAAACGCATATGATCACTTATTATCAAAGAATGGTGCTGTTTCGCAATCGCCAAAGCTCAGGCTACACCTTCTGCGATCAGTACTAGTGATACTTCATCAGTGGGCAATGTCTCTGTTTGCACACAGAATGAGTACTAGTGCTTATAGACCTTCTATTATTTTGGATGCAGCAAAAAGGTACATGTCTGAGGTGAGTGAGGTCCAAAGATTAGACCTTCCACAGATGCACCAGCAAACTGAAGCTGTTTACAGTGGCTTTCAAGAAATTGAAGAATCACTGAGACACAAGTCACCTATAAGAGTCACTGATCAGATATATTTCATTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000972 GO:0003674 GO:0005198 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005886 GO:0005911 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006998 GO:0008104 GO:0008150 GO:0008152 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010256 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016032 GO:0016043 GO:0017038 GO:0017056 GO:0030054 GO:0031090 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0036228 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044611 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051236 GO:0051276 GO:0051641 GO:0051649 GO:0051704 GO:0055044 GO:0061024 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071944 GO:0072594 GO:0072657 GO:0090435

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nucleoporin_C Nucleoporin_N

Protein Analysis

1087

Amino Acids

120.88

Weight (kDa)

6.4

Isoelectric Point (pI)

44.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nucleoporin_N	PF08801	45 - 232	1.5e-20	Nup133 N terminal like
Nucleoporin_N	PF08801	238 - 306	1.3e-07	Nup133 N terminal like
Nucleoporin_C	PF03177	547 - 1015	5.9e-31	Non-repetitive/WGA-negative nucleoporin C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14850
fragaria_vesca	FvH4_4g33560 FvH4_4g33560 FvH4_4g33560
malus_domestica	MD13G1020400.v1.1 MD16G1018600.v1.1 MD16G1018800.v1.1
prunus_persica	Prupe.1G333300_v2.0.a1
pyrus_communis	pycom13g01720 pycom16g01690
rosa_chinensis	RchiOBHm_Chr2g0167011 RchiOBHm_Chr2g0167021 RchiOBHm_Chr4g0443061
rosa_laevigata	RLG00000005957 RLG00000021689
rosa_multiflora	Rmu_co7980658.1_g000001 Rmu_sc0007968.1_g000017 Rmu_sc0008069.1_g000005 Rmu_sc0010966.1_g000010 Rmu_sc0039405.1_g000001 Rmu_sc0039405.1_g000002
rosa_roxburghii	Rroxscaffold_2G00083800 Rroxscaffold_5G00383690
rosa_rugosa	Rorug04G0344100
rosa_samantha	Rh2AG594500 Rh2AG594600 Rh2BG605800 Rh2BG605900 Rh2CG576700 Rh2DG617400 Rh4BG409900 Rh4DG404800
rosa_wichuraiana	Rw2G049490 Rw4G034330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	4 cut(s)	130, 185, 736, 2284
AccI	GTMKAC	2 cut(s)	536, 746
AciI	CCGC	8 cut(s)	503, 552, 702, 1253, 1319, 1434, 1610, 2567
AclWI	GGATC	1 cut(s)	2267
AcoI	YGGCCR	1 cut(s)	1516
AcsI	RAATTY	2 cut(s)	213, 1474
AcuI	CTGAAG	4 cut(s)	551, 1823, 2787, 3192
AcyI	GRCGYC	2 cut(s)	131, 2760
AdeI	CACNNNGTG	2 cut(s)	195, 2808
AfiI	CCNNNNNNNGG	6 cut(s)	362, 449, 1034, 1616, 2148, 2421
AflIII	ACRYGT	6 cut(s)	473, 586, 1012, 2077, 2158, 3114
AhlI	ACTAGT	2 cut(s)	3016, 3068
AjnI	CCWGG	2 cut(s)	81, 1205
AjuI	GAANNNNNNNTTGG	6 cut(s)	1638, 1670, 2517, 2549, 2816, 2848
AloI	GAACNNNNNNTCC	2 cut(s)	2350, 2382
Alw21I	GWGCWC	2 cut(s)	1382, 2110
Alw26I	GTCTC	3 cut(s)	768, 3048, 3208
Alw44I	GTGCAC	1 cut(s)	2106
AlwI	GGATC	1 cut(s)	2267
AlwNI	CAGNNNCTG	1 cut(s)	2855
AoxI	GGCC	8 cut(s)	79, 875, 959, 1163, 1507, 1516, 2242, 2610
ApaLI	GTGCAC	1 cut(s)	2106
ApoI	RAATTY	2 cut(s)	213, 1474
Asp700I	GAANNNNTTC	2 cut(s)	90, 381
AspLEI	GCGC	1 cut(s)	133
AspS9I	GGNCC	4 cut(s)	959, 1507, 1978, 3133
AsuHPI	GGTGA	8 cut(s)	37, 182, 332, 1259, 2033, 2321, 3136, 3216
AsuNHI	GCTAGC	1 cut(s)	1273
AvaII	GGWCC	2 cut(s)	1978, 3133
BaeGI	GKGCMC	2 cut(s)	2110, 2473
BanI	GGYRCC	4 cut(s)	130, 185, 736, 2284
BanII	GRGCYC	1 cut(s)	2890
BbsI	GAAGAC	2 cut(s)	1598, 1829
Bbv12I	GWGCWC	2 cut(s)	1382, 2110
BbvCI	CCTCAGC	2 cut(s)	1068, 1866
BceAI	ACGGC	3 cut(s)	323, 1503, 2208
BciT130I	CCWGG	2 cut(s)	83, 1207
BclI	TGATCA	3 cut(s)	1732, 2944, 3241
BcoDI	GTCTC	3 cut(s)	768, 3048, 3208
BcuI	ACTAGT	2 cut(s)	3016, 3068
BfmI	CTRYAG	4 cut(s)	885, 1050, 1772, 1897
BfoI	RGCGCY	1 cut(s)	134
BglI	GCCNNNNNGGC	1 cut(s)	1515
BlpI	GCTNAGC	1 cut(s)	1338
BmcAI	AGTACT	4 cut(s)	1770, 2216, 3015, 3067
Bme1390I	CCNGG	2 cut(s)	83, 1207
Bme18I	GGWCC	2 cut(s)	1978, 3133
BmgT120I	GGNCC	4 cut(s)	959, 1507, 1978, 3133
BmiI	GGNNCC	6 cut(s)	132, 187, 738, 1508, 2140, 2286
BmrFI	CCNGG	2 cut(s)	83, 1207
BmrI	ACTGGG	1 cut(s)	608
BmtI	GCTAGC	1 cut(s)	1277
BmuI	ACTGGG	1 cut(s)	608
BoxI	GACNNNNGTC	2 cut(s)	486, 3220
BpiI	GAAGAC	2 cut(s)	1598, 1829
BplI	GAGNNNNNCTC	2 cut(s)	979, 1011
BpmI	CTGGAG	1 cut(s)	384
Bpu10I	CCTNAGC	4 cut(s)	955, 1068, 1866, 2990
Bpu1102I	GCTNAGC	1 cut(s)	1338
BpuEI	CTTGAG	4 cut(s)	1481, 1572, 2594, 2840
BsaBI	GATNNNNATC	1 cut(s)	2558
BsaHI	GRCGYC	2 cut(s)	131, 2760
BsaJI	CCNNGG	5 cut(s)	750, 1133, 1205, 1206, 2907
BsaXI	ACNNNNNCTCC	4 cut(s)	746, 776, 2796, 2826
Bsc4I	CCNNNNNNNGG	6 cut(s)	362, 449, 1034, 1616, 2148, 2421
Bse118I	RCCGGY	3 cut(s)	53, 580, 2306
Bse1I	ACTGG	7 cut(s)	114, 614, 1028, 1295, 1531, 1549, 2847
Bse3DI	GCAATG	5 cut(s)	554, 678, 1218, 1473, 3045
Bse8I	GATNNNNATC	1 cut(s)	2558
BseBI	CCWGG	2 cut(s)	83, 1207
BseDI	CCNNGG	5 cut(s)	750, 1133, 1205, 1206, 2907
BseGI	GGATG	5 cut(s)	22, 517, 1291, 2184, 3103
BseJI	GATNNNNATC	1 cut(s)	2558
BseLI	CCNNNNNNNGG	6 cut(s)	362, 449, 1034, 1616, 2148, 2421
BseMI	GCAATG	5 cut(s)	554, 678, 1218, 1473, 3045
BseNI	ACTGG	7 cut(s)	114, 614, 1028, 1295, 1531, 1549, 2847
BseRI	GAGGAG	4 cut(s)	1195, 1346, 1691, 1883
BseSI	GKGCMC	2 cut(s)	2110, 2473
BseYI	CCCAGC	1 cut(s)	1280
BsgI	GTGCAG	2 cut(s)	1468, 1785
BshFI	GGCC	8 cut(s)	81, 877, 961, 1165, 1509, 1518, 2244, 2612
BshNI	GGYRCC	4 cut(s)	130, 185, 736, 2284
BsiHKAI	GWGCWC	2 cut(s)	1382, 2110
BsiSI	CCGG	3 cut(s)	54, 581, 2307
BslFI	GGGAC	2 cut(s)	988, 1759
BslI	CCNNNNNNNGG	6 cut(s)	362, 449, 1034, 1616, 2148, 2421
BsmAI	GTCTC	3 cut(s)	768, 3048, 3208
BsmFI	GGGAC	2 cut(s)	988, 1759
BsnI	GGCC	8 cut(s)	81, 877, 961, 1165, 1509, 1518, 2244, 2612
Bsp1286I	GDGCHC	4 cut(s)	1382, 2110, 2473, 2890
Bsp1407I	TGTACA	1 cut(s)	1357
Bsp143I	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
Bsp1720I	GCTNAGC	1 cut(s)	1338
Bsp19I	CCATGG	1 cut(s)	750
BspACI	CCGC	8 cut(s)	503, 552, 702, 1253, 1319, 1434, 1610, 2567
BspANI	GGCC	8 cut(s)	81, 877, 961, 1165, 1509, 1518, 2244, 2612
BspHI	TCATGA	1 cut(s)	2209
BspLI	GGNNCC	6 cut(s)	132, 187, 738, 1508, 2140, 2286
BspMAI	CTGCAG	1 cut(s)	1776
BspOI	GCTAGC	1 cut(s)	1277
BspPI	GGATC	1 cut(s)	2267
BspQI	GCTCTTC	1 cut(s)	2895
BspT107I	GGYRCC	4 cut(s)	130, 185, 736, 2284
BsrDI	GCAATG	5 cut(s)	554, 678, 1218, 1473, 3045
BsrFI	RCCGGY	3 cut(s)	53, 580, 2306
BsrGI	TGTACA	1 cut(s)	1357
BsrI	ACTGG	7 cut(s)	114, 614, 1028, 1295, 1531, 1549, 2847
BssAI	RCCGGY	3 cut(s)	53, 580, 2306
BssECI	CCNNGG	5 cut(s)	750, 1133, 1205, 1206, 2907
BssMI	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
BssNAI	GTATAC	1 cut(s)	537
BssNI	GRCGYC	2 cut(s)	131, 2760
BssT1I	CCWWGG	3 cut(s)	750, 1133, 2907
Bst1107I	GTATAC	1 cut(s)	537
Bst2UI	CCWGG	2 cut(s)	83, 1207
Bst4CI	ACNGT	6 cut(s)	444, 1750, 1901, 2501, 2808, 3185
Bst6I	CTCTTC	3 cut(s)	2149, 2780, 2895
BstACI	GRCGYC	2 cut(s)	131, 2760
BstAPI	GCANNNNNTGC	1 cut(s)	2444
BstAUI	TGTACA	1 cut(s)	1357
BstC8I	GCNNGC	7 cut(s)	55, 582, 879, 1109, 1275, 2436, 2925
BstDSI	CCRYGG	1 cut(s)	750
BstEII	GGTNACC	1 cut(s)	1682
BstF5I	GGATG	5 cut(s)	22, 517, 1291, 2184, 3103
BstH2I	RGCGCY	1 cut(s)	134
BstHHI	GCGC	1 cut(s)	133
BstKTI	GATC	7 cut(s)	1158, 1735, 2275, 2562, 2947, 3011, 3244
BstMAI	GTCTC	3 cut(s)	768, 3048, 3208
BstMBI	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
BstMWI	GCNNNNNNNGC	9 cut(s)	139, 665, 1138, 1250, 1515, 1892, 2431, 2444, 2894
BstNI	CCWGG	2 cut(s)	83, 1207
BstNSI	RCATGY	5 cut(s)	477, 590, 1016, 2162, 3118
BstPAI	GACNNNNGTC	2 cut(s)	486, 3220
BstPI	GGTNACC	1 cut(s)	1682
BstSCI	CCNGG	2 cut(s)	81, 1205
BstSFI	CTRYAG	4 cut(s)	885, 1050, 1772, 1897
BstSLI	GKGCMC	2 cut(s)	2110, 2473
BstV2I	GAAGAC	2 cut(s)	1598, 1829
BstX2I	RGATCY	1 cut(s)	2272
BstXI	CCANNNNNNTGG	1 cut(s)	77
BstYI	RGATCY	1 cut(s)	2272
BstZ17I	GTATAC	1 cut(s)	537
BsuRI	GGCC	8 cut(s)	81, 877, 961, 1165, 1509, 1518, 2244, 2612
BtgI	CCRYGG	1 cut(s)	750
BtgZI	GCGATG	2 cut(s)	54, 1274
BtsCI	GGATG	5 cut(s)	22, 517, 1291, 2184, 3103
BtsI	GCAGTG	2 cut(s)	1980, 2108
BtsIMutI	CAGTG	8 cut(s)	1980, 2090, 2108, 2804, 3038, 3190, 3209, 3237
Cac8I	GCNNGC	7 cut(s)	55, 582, 879, 1109, 1275, 2436, 2925
CaiI	CAGNNNCTG	1 cut(s)	2855
CciI	TCATGA	1 cut(s)	2209
CfoI	GCGC	1 cut(s)	133
Cfr10I	RCCGGY	3 cut(s)	53, 580, 2306
Cfr13I	GGNCC	4 cut(s)	959, 1507, 1978, 3133
CseI	GACGC	1 cut(s)	2749
DinI	GGCGCC	1 cut(s)	132
DpnI	GATC	7 cut(s)	1157, 1734, 2274, 2561, 2946, 3010, 3243
DpnII	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
DraI	TTTAAA	1 cut(s)	1854
DraIII	CACNNNGTG	2 cut(s)	195, 2808
EaeI	YGGCCR	1 cut(s)	1516
Eam1104I	CTCTTC	3 cut(s)	2149, 2780, 2895
EarI	CTCTTC	3 cut(s)	2149, 2780, 2895
Eco130I	CCWWGG	3 cut(s)	750, 1133, 2907
Eco147I	AGGCCT	2 cut(s)	877, 1165
Eco24I	GRGCYC	1 cut(s)	2890
Eco32I	GATATC	1 cut(s)	2691
Eco47I	GGWCC	2 cut(s)	1978, 3133
Eco57I	CTGAAG	4 cut(s)	551, 1823, 2787, 3192
Eco91I	GGTNACC	1 cut(s)	1682
EcoO109I	RGGNCCY	1 cut(s)	1507
EcoO65I	GGTNACC	1 cut(s)	1682
EcoRII	CCWGG	2 cut(s)	81, 1205
EcoRV	GATATC	1 cut(s)	2691
EcoT14I	CCWWGG	3 cut(s)	750, 1133, 2907
EcoT22I	ATGCAT	1 cut(s)	2449
EcoT38I	GRGCYC	1 cut(s)	2890
EgeI	GGCGCC	1 cut(s)	132
EheI	GGCGCC	1 cut(s)	132
ErhI	CCWWGG	3 cut(s)	750, 1133, 2907
FalI	AAGNNNNNCTT	6 cut(s)	2517, 2549, 2556, 2588, 2704, 2736
FaqI	GGGAC	2 cut(s)	988, 1759
FauI	CCCGC	2 cut(s)	496, 695
FauNDI	CATATG	1 cut(s)	2941
FbaI	TGATCA	3 cut(s)	1732, 2944, 3241
FblI	GTMKAC	2 cut(s)	536, 746
FokI	GGATG	5 cut(s)	29, 524, 1278, 2171, 3110
FriOI	GRGCYC	1 cut(s)	2890
GlaI	GCGC	1 cut(s)	132
GsaI	CCCAGC	1 cut(s)	1284
GsuI	CTGGAG	1 cut(s)	384
HaeII	RGCGCY	1 cut(s)	134
HaeIII	GGCC	8 cut(s)	81, 877, 961, 1165, 1509, 1518, 2244, 2612
HapII	CCGG	3 cut(s)	54, 581, 2307
HgaI	GACGC	1 cut(s)	2749
HhaI	GCGC	1 cut(s)	133
Hin1I	GRCGYC	2 cut(s)	131, 2760
Hin6I	GCGC	1 cut(s)	131
HinP1I	GCGC	1 cut(s)	131
HincII	GTYRAC	1 cut(s)	610
HindII	GTYRAC	1 cut(s)	610
HindIII	AAGCTT	3 cut(s)	140, 2718, 2769
HpaII	CCGG	3 cut(s)	54, 581, 2307
HphI	GGTGA	8 cut(s)	37, 182, 332, 1259, 2033, 2321, 3136, 3216
Hpy166II	GTNNAC	8 cut(s)	440, 537, 610, 747, 1981, 2108, 2312, 3181
Hpy8I	GTNNAC	8 cut(s)	440, 537, 610, 747, 1981, 2108, 2312, 3181
Hpy99I	CGWCG	1 cut(s)	2765
HpyCH4III	ACNGT	6 cut(s)	444, 1750, 1901, 2501, 2808, 3185
HpyCH4IV	ACGT	2 cut(s)	1603, 2077
HpyF10VI	GCNNNNNNNGC	9 cut(s)	139, 665, 1138, 1250, 1515, 1892, 2431, 2444, 2894
HpySE526I	ACGT	2 cut(s)	1603, 2077
Hsp92I	GRCGYC	2 cut(s)	131, 2760
HspAI	GCGC	1 cut(s)	131
KasI	GGCGCC	1 cut(s)	130
KroI	GCCGGC	2 cut(s)	53, 580
KroNI	GCCGGC	2 cut(s)	55, 582
Ksp22I	TGATCA	3 cut(s)	1732, 2944, 3241
Kzo9I	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
LguI	GCTCTTC	1 cut(s)	2895
LmnI	GCTCC	4 cut(s)	248, 886, 1241, 1602
MaeII	ACGT	2 cut(s)	1603, 2077
MalI	GATC	7 cut(s)	1157, 1734, 2274, 2561, 2946, 3010, 3243
MboI	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
MfeI	CAATTG	1 cut(s)	1646
MflI	RGATCY	1 cut(s)	2272
MhlI	GDGCHC	4 cut(s)	1382, 2110, 2473, 2890
Mly113I	GGCGCC	1 cut(s)	131
MlyI	GAGTC	7 cut(s)	764, 870, 1081, 1811, 2606, 2647, 3243
MmeI	TCCRAC	6 cut(s)	322, 470, 538, 1624, 2393, 2502
Mph1103I	ATGCAT	1 cut(s)	2449
MroNI	GCCGGC	2 cut(s)	53, 580
MroXI	GAANNNNTTC	2 cut(s)	90, 381
MslI	CAYNNNNRTG	1 cut(s)	522
MspA1I	CMGCKG	3 cut(s)	1280, 1355, 2518
MspI	CCGG	3 cut(s)	54, 581, 2307
MspR9I	CCNGG	2 cut(s)	83, 1207
MunI	CAATTG	1 cut(s)	1646
MvaI	CCWGG	2 cut(s)	83, 1207
MwoI	GCNNNNNNNGC	9 cut(s)	139, 665, 1138, 1250, 1515, 1892, 2431, 2444, 2894
NaeI	GCCGGC	2 cut(s)	55, 582
NarI	GGCGCC	1 cut(s)	131
NcoI	CCATGG	1 cut(s)	750
NdeI	CATATG	1 cut(s)	2941
NdeII	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
NgoMIV	GCCGGC	2 cut(s)	53, 580
NheI	GCTAGC	1 cut(s)	1273
NlaIV	GGNNCC	6 cut(s)	132, 187, 738, 1508, 2140, 2286
NmeAIII	GCCGAG	1 cut(s)	1361
NmuCI	GTSAC	5 cut(s)	43, 479, 865, 3222, 3235
NsiI	ATGCAT	1 cut(s)	2449
NspI	RCATGY	5 cut(s)	477, 590, 1016, 2162, 3118
PagI	TCATGA	1 cut(s)	2209
PasI	CCCWGGG	1 cut(s)	1206
PceI	AGGCCT	2 cut(s)	877, 1165
PciI	ACATGT	5 cut(s)	473, 586, 1012, 2158, 3114
PciSI	GCTCTTC	1 cut(s)	2895
PcsI	WCGNNNNNNNCGW	1 cut(s)	36
PdiI	GCCGGC	2 cut(s)	55, 582
PdmI	GAANNNNTTC	2 cut(s)	90, 381
PfeI	GAWTC	7 cut(s)	679, 941, 1042, 1559, 2846, 2858, 3206
PleI	GAGTC	7 cut(s)	763, 869, 1081, 1810, 2605, 2646, 3242
PluTI	GGCGCC	1 cut(s)	134
PpsI	GAGTC	7 cut(s)	763, 869, 1081, 1810, 2605, 2646, 3242
PscI	ACATGT	5 cut(s)	473, 586, 1012, 2158, 3114
PshAI	GACNNNNGTC	2 cut(s)	486, 3220
Psp6I	CCWGG	2 cut(s)	81, 1205
PspEI	GGTNACC	1 cut(s)	1682
PspFI	CCCAGC	1 cut(s)	1280
PspGI	CCWGG	2 cut(s)	81, 1205
PspN4I	GGNNCC	6 cut(s)	132, 187, 738, 1508, 2140, 2286
PspPI	GGNCC	4 cut(s)	959, 1507, 1978, 3133
PstI	CTGCAG	1 cut(s)	1776
PstNI	CAGNNNCTG	1 cut(s)	2855
PsuI	RGATCY	1 cut(s)	2272
PvuII	CAGCTG	3 cut(s)	1280, 1355, 2518
RseI	CAYNNNNRTG	1 cut(s)	522
SapI	GCTCTTC	1 cut(s)	2895
Sau3AI	GATC	7 cut(s)	1155, 1732, 2272, 2559, 2944, 3008, 3241
Sau96I	GGNCC	4 cut(s)	959, 1507, 1978, 3133
ScaI	AGTACT	4 cut(s)	1770, 2216, 3015, 3067
SchI	GAGTC	7 cut(s)	764, 870, 1081, 1811, 2606, 2647, 3243
ScrFI	CCNGG	2 cut(s)	83, 1207
SduI	GDGCHC	4 cut(s)	1382, 2110, 2473, 2890
SfcI	CTRYAG	4 cut(s)	885, 1050, 1772, 1897
SfiI	GGCCNNNNNGGCC	1 cut(s)	1515
SfoI	GGCGCC	1 cut(s)	132
SinI	GGWCC	2 cut(s)	1978, 3133
SmiMI	CAYNNNNRTG	1 cut(s)	522
SmlI	CTYRAG	4 cut(s)	1460, 1587, 2573, 2819
SmoI	CTYRAG	4 cut(s)	1460, 1587, 2573, 2819
SpeI	ACTAGT	2 cut(s)	3016, 3068
SseBI	AGGCCT	2 cut(s)	877, 1165
SsiI	CCGC	8 cut(s)	503, 552, 702, 1253, 1319, 1434, 1610, 2567
SspDI	GGCGCC	1 cut(s)	130
SspI	AATATT	2 cut(s)	204, 2387
StuI	AGGCCT	2 cut(s)	877, 1165
StyD4I	CCNGG	2 cut(s)	81, 1205
StyI	CCWWGG	3 cut(s)	750, 1133, 2907
TaaI	ACNGT	6 cut(s)	444, 1750, 1901, 2501, 2808, 3185
TaiI	ACGT	2 cut(s)	1606, 2080
TaqI	TCGA	4 cut(s)	285, 633, 1154, 2056
TauI	GCSGC	1 cut(s)	1256
TfiI	GAWTC	7 cut(s)	679, 941, 1042, 1559, 2846, 2858, 3206
TscAI	CASTG	8 cut(s)	1980, 2090, 2115, 2811, 3038, 3190, 3216, 3244
TseFI	GTSAC	5 cut(s)	43, 479, 865, 3222, 3235
Tsp45I	GTSAC	5 cut(s)	43, 479, 865, 3222, 3235
TspGWI	ACGGA	1 cut(s)	1254
TspRI	CASTG	8 cut(s)	1980, 2090, 2115, 2811, 3038, 3190, 3216, 3244
VneI	GTGCAC	1 cut(s)	2106
VpaK11BI	GGWCC	2 cut(s)	1978, 3133
XapI	RAATTY	2 cut(s)	213, 1474
XbaI	TCTAGA	1 cut(s)	2695
XceI	RCATGY	5 cut(s)	477, 590, 1016, 2162, 3118
XcmI	CCANNNNNNNNNTGG	2 cut(s)	55, 2679
XmiI	GTMKAC	2 cut(s)	536, 746
XmnI	GAANNNNTTC	2 cut(s)	90, 381
ZrmI	AGTACT	4 cut(s)	1770, 2216, 3015, 3067
Zsp2I	ATGCAT	1 cut(s)	2449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.