Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002985

Physical Location & Seq

Genomic Coordinates Reverse (-)

2634615 .. 2638469

Gene Structure 3855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1039000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGGCAGCGCACCACAAGCTAGCTGTGATTCTCGAGAATCCTCAAAACGACGCCGAATTCCTCCTCGTGAAACAGACGCGACCGCCGAAGTTCGACTGCGATGAGTACGACTCGTACGTCGATTCTGATCTCTGGGACTTGCCATCCTCCCAATTGAACCTTGTAGAAGGACTATCGAAGCCCTCGATTGCAGTGGCAGGCGCAGAACCGTGGTCGGGGAAGATCGATTTGGCAAAATTCGACTTCGAGTCGGCTCTCAACCAGGTTCTGGAACAAGTAGGGTCTAAGGCGGCTGACGGTGGAGATTGGAGGTTGTCCAAGTACGTGGAGGAAGCTCAGTTCGGACCCGGGCCGCCAATCAATTCGGTGTTTATTAGGGGAAAATTGGTAGCTTCGGAGCAAAATTTACAAGAATCGTGTAAATGGATGTCTGTCCCGAGTTGTCTAAGCTGGCTTCTTGAAGTGAAACCAAGCAGTGATCGTGTGGGGCCGTTGATAGTTGCCGGTCTTCTAAATGAATCTGAGAAATCGAGAGAGATGAACATTCCATCCACCTTACCTTACCAGGAGTACCCTCCTGGTGTTGTGCTTGTACCTATGGGAAGCAGGACAGGAAAACCTTTTTATACAACAAATCTGATTGTTTTTGCACCCAAAAATGCTTCAAATGAATTTGTAGAGGATGATTTTATTGCTTGTGGAGATGCATTGATAGTGGACCCTGGATGCCGGTCTGAATTTCATGAAGAGCTCGCACAAATTGTAACTTCATTGCCAAGAAAGTTAGTTGTCTTTGTTACTCATCACCATCGCGATCATGTAGATGGTCTTTCAGTTATTCAGCGTTGCAATCCTGATGCTACTTTATTAGCACATGAAAATACCATGCGACGCATTGGAAAAGATGACTGGTCTCTTGGCTTCACTGCAATTTCTGGAACTGAAGAGATTTGCATTGGTGGTCAGCGATTGATTGCCGTTTTTTCTCCGGGGCATACAGATGGGCATATGGCACTGCTCCATGCTAGTACTCACTCATTGATTGTTGGCGATCATTGTGTGGGTCAAGGTAGTGCTGTACTGGATGTTACTGGTGGGGGAAATATGACTGATTATTTTCAATCAACTTACAAATTCATGGAGCTTTCCCCACATGCCTTGGTCCCCATTCATGGGAGAGTTAACCTATGGCCAAAGCACATGCTTTGTGGATATCTCAAGAACCGCAGAGCAAGAGAAAGTTCTGTCTTGAAAGCGATAGAAAATGGAGCAGAAACATTGTTTGACATAGTTTCCACTGTATATTCGGAGATTGATCGTAGCATTTGGATTCATTGTGCATCTAATGTGAGGCTTCATGTGGATCATCTTGCCCAGCAAGATAAGTTACCAAAGGTAACATTTTCCTGGTTTCACGACGTAATCTTTAACTTGGTCCTACATCAATGCAACTTTAGCATGCACGTAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0031974 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046872 GO:0046914 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lactamase_B

Protein Analysis

491

Amino Acids

54.28

Weight (kDa)

5.61

Isoelectric Point (pI)

39.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	234 - 377	4.7e-07	Metallo-beta-lactamase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25375
fragaria_vesca	FvH4_4g26975 FvH4_4g26975 FvH4_4g26975 FvH4_4g26975
malus_domestica	MD13G1039000.v1.1 MD16G1039700.v1.1
prunus_persica	Prupe.1G314400_v2.0.a1 Prupe.1G314400_v2.0.a1
pyrus_communis	pycom16g03450
rosa_chinensis	RchiOBHm_Chr2g0108931 RchiOBHm_Chr4g0434781 RchiOBHm_Chr5g0042931 RchiOBHm_Chr6g0261541 RchiOBHm_Chr7g0243441
rosa_laevigata	RLG00000006650
rosa_multiflora	Rmu_sc0007829.1_g000001 Rmu_sc0017904.1_g000001 Rmu_sc0017904.1_g000002 Rmu_sc0026857.1_g000001
rosa_roxburghii	Rroxscaffold_4G00312600 Rroxscaffold_5G00375980
rosa_rugosa	Rorug02G0079600 Rorug04G0278300 Rorug06G0479000
rosa_samantha	Rh2AG012800 Rh2BG218400 Rh2CG210400 Rh2DG354000 Rh2DG354100 Rh4AG334700 Rh4BG343300 Rh4CG357700 Rh4DG337000 Rh7BG078500
rosa_wichuraiana	Rw1G015910 Rw4G029190 Rw7G037630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	116
AccB7I	CCANNNNNTGG	1 cut(s)	268
AccII	CGCG	2 cut(s)	79, 813
AciI	CCGC	4 cut(s)	83, 290, 353, 1225
AclWI	GGATC	1 cut(s)	1371
AcoI	YGGCCR	1 cut(s)	1190
AcsI	RAATTY	6 cut(s)	56, 236, 403, 671, 737, 1133
AcuI	CTGAAG	1 cut(s)	963
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	7 cut(s)	107, 116, 323, 572, 594, 1030, 1080
AfiI	CCNNNNNNNGG	4 cut(s)	215, 268, 1172, 1173
AgsI	TTSAA	5 cut(s)	157, 461, 666, 1121, 1252
AhdI	GACNNNNNGTC	1 cut(s)	247
AjnI	CCWGG	5 cut(s)	261, 564, 577, 721, 1406
AjuI	GAANNNNNNNTTGG	2 cut(s)	212, 244
AloI	GAACNNNNNNTCC	4 cut(s)	323, 355, 533, 565
AluBI	AGCT	7 cut(s)	19, 23, 335, 392, 450, 751, 1144
AluI	AGCT	7 cut(s)	19, 23, 335, 392, 450, 751, 1144
Alw21I	GWGCWC	1 cut(s)	753
Alw26I	GTCTC	1 cut(s)	918
AlwI	GGATC	1 cut(s)	1371
AlwNI	CAGNNNCTG	1 cut(s)	268
Ama87I	CYCGRG	3 cut(s)	32, 347, 436
AoxI	GGCC	3 cut(s)	350, 488, 1190
ApeKI	GCWGC	1 cut(s)	5
ApoI	RAATTY	6 cut(s)	56, 236, 403, 671, 737, 1133
AspLEI	GCGC	2 cut(s)	10, 203
AspS9I	GGNCC	6 cut(s)	344, 350, 488, 718, 1162, 1435
AsuC2I	CCSGG	3 cut(s)	348, 349, 990
AsuHPI	GGTGA	1 cut(s)	797
AsuNHI	GCTAGC	1 cut(s)	19
AvaI	CYCGRG	3 cut(s)	32, 347, 436
AvaII	GGWCC	4 cut(s)	344, 718, 1162, 1435
BalI	TGGCCA	1 cut(s)	1192
BanII	GRGCYC	1 cut(s)	753
BauI	CACGAG	1 cut(s)	65
BbsI	GAAGAC	1 cut(s)	500
Bbv12I	GWGCWC	1 cut(s)	753
BbvI	GCAGC	1 cut(s)	17
BccI	CCATC	5 cut(s)	151, 556, 816, 818, 995
BceAI	ACGGC	2 cut(s)	475, 962
BciT130I	CCWGG	5 cut(s)	263, 566, 579, 723, 1408
BcnI	CCSGG	3 cut(s)	348, 349, 990
BcoDI	GTCTC	1 cut(s)	918
BfaI	CTAG	2 cut(s)	20, 1026
BisI	GCNGC	3 cut(s)	6, 291, 353
BlsI	GCNGC	3 cut(s)	7, 292, 354
BmcAI	AGTACT	1 cut(s)	1030
Bme1390I	CCNGG	8 cut(s)	263, 348, 349, 566, 579, 723, 990, 1408
Bme18I	GGWCC	4 cut(s)	344, 718, 1162, 1435
BmeRI	GACNNNNNGTC	1 cut(s)	247
BmeT110I	CYCGRG	3 cut(s)	32, 347, 436
BmgT120I	GGNCC	6 cut(s)	344, 350, 488, 718, 1162, 1435
BmiI	GGNNCC	4 cut(s)	346, 489, 720, 1164
BmrFI	CCNGG	8 cut(s)	263, 348, 349, 566, 579, 723, 990, 1408
BmsI	GCATC	4 cut(s)	694, 716, 847, 1349
BmtI	GCTAGC	1 cut(s)	23
BpiI	GAAGAC	1 cut(s)	500
BplI	GAGNNNNNCTC	2 cut(s)	95, 127
BpuEI	CTTGAG	1 cut(s)	1202
BpuMI	CCSGG	3 cut(s)	348, 349, 990
Bsa29I	ATCGAT	1 cut(s)	225
BsaAI	YACGTR	2 cut(s)	325, 1465
BsaBI	GATNNNNATC	1 cut(s)	126
BsaHI	GRCGYC	1 cut(s)	51
BsaI	GGTCTC	1 cut(s)	918
BsaJI	CCNNGG	5 cut(s)	209, 347, 721, 989, 1158
Bsc4I	CCNNNNNNNGG	4 cut(s)	215, 268, 1172, 1173
Bse118I	RCCGGY	2 cut(s)	503, 729
Bse1I	ACTGG	3 cut(s)	914, 1086, 1096
Bse3DI	GCAATG	1 cut(s)	770
Bse8I	GATNNNNATC	1 cut(s)	126
BseBI	CCWGG	5 cut(s)	263, 566, 579, 723, 1408
BseCI	ATCGAT	1 cut(s)	225
BseDI	CCNNGG	5 cut(s)	209, 347, 721, 989, 1158
BseGI	GGATG	6 cut(s)	143, 432, 548, 688, 731, 1090
BseJI	GATNNNNATC	1 cut(s)	126
BseLI	CCNNNNNNNGG	4 cut(s)	215, 268, 1172, 1173
BseMI	GCAATG	1 cut(s)	770
BseMII	CTCAG	2 cut(s)	350, 513
BseNI	ACTGG	3 cut(s)	914, 1086, 1096
BseRI	GAGGAG	1 cut(s)	53
BseXI	GCAGC	1 cut(s)	17
BseYI	CCCAGC	1 cut(s)	1374
Bsh1236I	CGCG	2 cut(s)	79, 813
Bsh1285I	CGRYCG	1 cut(s)	83
BshFI	GGCC	3 cut(s)	352, 490, 1192
BshVI	ATCGAT	1 cut(s)	225
BsiEI	CGRYCG	1 cut(s)	83
BsiHKAI	GWGCWC	1 cut(s)	753
BsiHKCI	CYCGRG	3 cut(s)	32, 347, 436
BsiSI	CCGG	4 cut(s)	348, 504, 730, 989
BsiWI	CGTACG	1 cut(s)	114
BslFI	GGGAC	3 cut(s)	149, 419, 1148
BslI	CCNNNNNNNGG	4 cut(s)	215, 268, 1172, 1173
BsmAI	GTCTC	1 cut(s)	918
BsmFI	GGGAC	3 cut(s)	149, 419, 1148
BsnI	GGCC	3 cut(s)	352, 490, 1192
Bso31I	GGTCTC	1 cut(s)	918
BsoBI	CYCGRG	3 cut(s)	32, 347, 436
Bsp1286I	GDGCHC	1 cut(s)	753
Bsp143I	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
Bsp68I	TCGCGA	1 cut(s)	813
BspACI	CCGC	4 cut(s)	83, 290, 353, 1225
BspANI	GGCC	3 cut(s)	352, 490, 1192
BspCNI	CTCAG	2 cut(s)	349, 514
BspDI	ATCGAT	1 cut(s)	225
BspFNI	CGCG	2 cut(s)	79, 813
BspHI	TCATGA	1 cut(s)	742
BspLI	GGNNCC	4 cut(s)	346, 489, 720, 1164
BspOI	GCTAGC	1 cut(s)	23
BspPI	GGATC	1 cut(s)	1371
BspQI	GCTCTTC	1 cut(s)	741
BspTNI	GGTCTC	1 cut(s)	918
BsrDI	GCAATG	1 cut(s)	770
BsrFI	RCCGGY	2 cut(s)	503, 729
BsrI	ACTGG	3 cut(s)	914, 1086, 1096
BssAI	RCCGGY	2 cut(s)	503, 729
BssECI	CCNNGG	5 cut(s)	209, 347, 721, 989, 1158
BssMI	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
BssNI	GRCGYC	1 cut(s)	51
BssSI	CACGAG	1 cut(s)	65
BssT1I	CCWWGG	1 cut(s)	1158
Bst2BI	CACGAG	1 cut(s)	65
Bst2UI	CCWGG	5 cut(s)	263, 566, 579, 723, 1408
Bst4CI	ACNGT	3 cut(s)	210, 299, 1300
Bst6I	CTCTTC	2 cut(s)	741, 939
BstACI	GRCGYC	1 cut(s)	51
BstBAI	YACGTR	2 cut(s)	325, 1465
BstC8I	GCNNGC	5 cut(s)	21, 199, 452, 753, 1460
BstDEI	CTNAG	4 cut(s)	285, 336, 446, 522
BstDSI	CCRYGG	1 cut(s)	209
BstF5I	GGATG	6 cut(s)	143, 432, 548, 688, 731, 1090
BstFNI	CGCG	2 cut(s)	79, 813
BstHHI	GCGC	2 cut(s)	10, 203
BstKTI	GATC	7 cut(s)	130, 225, 481, 817, 1054, 1318, 1366
BstMAI	GTCTC	1 cut(s)	918
BstMBI	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
BstMCI	CGRYCG	1 cut(s)	83
BstMWI	GCNNNNNNNGC	2 cut(s)	16, 1455
BstNI	CCWGG	5 cut(s)	263, 566, 579, 723, 1408
BstNSI	RCATGY	3 cut(s)	1157, 1204, 1462
BstSCI	CCNGG	8 cut(s)	261, 346, 347, 564, 577, 721, 988, 1406
BstUI	CGCG	2 cut(s)	79, 813
BstV1I	GCAGC	1 cut(s)	17
BstV2I	GAAGAC	1 cut(s)	500
BstXI	CCANNNNNNTGG	1 cut(s)	325
Bsu15I	ATCGAT	1 cut(s)	225
BsuRI	GGCC	3 cut(s)	352, 490, 1192
BsuTUI	ATCGAT	1 cut(s)	225
BtgI	CCRYGG	1 cut(s)	209
BtgZI	GCGATG	2 cut(s)	114, 794
BtsCI	GGATG	6 cut(s)	143, 432, 548, 688, 731, 1090
BtsI	GCAGTG	4 cut(s)	198, 481, 924, 1013
BtsIMutI	CAGTG	5 cut(s)	198, 481, 924, 1013, 1296
BtuMI	TCGCGA	1 cut(s)	813
Cac8I	GCNNGC	5 cut(s)	21, 199, 452, 753, 1460
CaiI	CAGNNNCTG	1 cut(s)	268
CciI	TCATGA	1 cut(s)	742
CfoI	GCGC	2 cut(s)	10, 203
Cfr10I	RCCGGY	2 cut(s)	503, 729
Cfr13I	GGNCC	6 cut(s)	344, 350, 488, 718, 1162, 1435
Cfr9I	CCCGGG	1 cut(s)	347
ClaI	ATCGAT	1 cut(s)	225
CseI	GACGC	3 cut(s)	59, 85, 900
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	7 cut(s)	106, 115, 322, 571, 593, 1029, 1079
CspCI	CAANNNNNGTGG	2 cut(s)	1140, 1175
CviQI	GTAC	7 cut(s)	106, 115, 322, 571, 593, 1029, 1079
DdeI	CTNAG	4 cut(s)	285, 336, 446, 522
DpnI	GATC	7 cut(s)	129, 224, 480, 816, 1053, 1317, 1365
DpnII	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
DrdI	GACNNNNNNGTC	1 cut(s)	116
DriI	GACNNNNNGTC	1 cut(s)	247
DseDI	GACNNNNNNGTC	1 cut(s)	116
EaeI	YGGCCR	1 cut(s)	1190
Eam1104I	CTCTTC	2 cut(s)	741, 939
Eam1105I	GACNNNNNGTC	1 cut(s)	247
EarI	CTCTTC	2 cut(s)	741, 939
Ecl136II	GAGCTC	1 cut(s)	751
Eco130I	CCWWGG	1 cut(s)	1158
Eco24I	GRGCYC	1 cut(s)	753
Eco31I	GGTCTC	1 cut(s)	918
Eco32I	GATATC	1 cut(s)	1214
Eco47I	GGWCC	4 cut(s)	344, 718, 1162, 1435
Eco53kI	GAGCTC	1 cut(s)	751
Eco57I	CTGAAG	1 cut(s)	963
Eco88I	CYCGRG	3 cut(s)	32, 347, 436
EcoICRI	GAGCTC	1 cut(s)	751
EcoRI	GAATTC	1 cut(s)	56
EcoRII	CCWGG	5 cut(s)	261, 564, 577, 721, 1406
EcoRV	GATATC	1 cut(s)	1214
EcoT14I	CCWWGG	1 cut(s)	1158
EcoT22I	ATGCAT	1 cut(s)	709
EcoT38I	GRGCYC	1 cut(s)	753
ErhI	CCWWGG	1 cut(s)	1158
FaqI	GGGAC	3 cut(s)	149, 419, 1148
FauNDI	CATATG	1 cut(s)	1008
Fnu4HI	GCNGC	3 cut(s)	6, 291, 353
FokI	GGATG	6 cut(s)	130, 439, 535, 695, 738, 1097
FriOI	GRGCYC	1 cut(s)	753
Fsp4HI	GCNGC	3 cut(s)	6, 291, 353
FspBI	CTAG	2 cut(s)	20, 1026
GlaI	GCGC	2 cut(s)	9, 202
GluI	GCNGC	3 cut(s)	6, 291, 353
GsaI	CCCAGC	1 cut(s)	1378
HaeIII	GGCC	3 cut(s)	352, 490, 1192
HapII	CCGG	4 cut(s)	348, 504, 730, 989
HgaI	GACGC	3 cut(s)	59, 85, 900
HhaI	GCGC	2 cut(s)	10, 203
Hin1I	GRCGYC	1 cut(s)	51
Hin6I	GCGC	2 cut(s)	8, 201
HinP1I	GCGC	2 cut(s)	8, 201
HincII	GTYRAC	1 cut(s)	1183
HindII	GTYRAC	1 cut(s)	1183
HinfI	GANTC	8 cut(s)	28, 37, 110, 122, 248, 413, 518, 1330
HpaI	GTTAAC	1 cut(s)	1183
HpaII	CCGG	4 cut(s)	348, 504, 730, 989
HphI	GGTGA	1 cut(s)	797
Hpy166II	GTNNAC	2 cut(s)	718, 1183
Hpy188I	TCNGA	7 cut(s)	127, 344, 397, 523, 639, 736, 1309
Hpy8I	GTNNAC	2 cut(s)	718, 1183
Hpy99I	CGWCG	4 cut(s)	53, 122, 894, 1421
HpyAV	CCTTC	1 cut(s)	161
HpyCH4III	ACNGT	3 cut(s)	210, 299, 1300
HpyCH4IV	ACGT	4 cut(s)	117, 324, 1419, 1464
HpyCH4V	TGCA	9 cut(s)	191, 650, 707, 849, 929, 954, 1340, 1449, 1462
HpyF10VI	GCNNNNNNNGC	2 cut(s)	16, 1455
HpyF3I	CTNAG	4 cut(s)	285, 336, 446, 522
HpySE526I	ACGT	4 cut(s)	117, 324, 1419, 1464
Hsp92I	GRCGYC	1 cut(s)	51
HspAI	GCGC	2 cut(s)	8, 201
KspAI	GTTAAC	1 cut(s)	1183
Kzo9I	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	4 cut(s)	397, 1023, 1141, 1268
Lsp1109I	GCAGC	1 cut(s)	17
LweI	GCATC	4 cut(s)	694, 716, 847, 1349
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	2 cut(s)	20, 1026
MaeII	ACGT	4 cut(s)	117, 324, 1419, 1464
MaeIII	GTNAC	5 cut(s)	763, 796, 1087, 1386, 1396
MalI	GATC	7 cut(s)	129, 224, 480, 816, 1053, 1317, 1365
MboI	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
MboII	GAAGA	4 cut(s)	232, 500, 758, 956
MfeI	CAATTG	1 cut(s)	152
MhlI	GDGCHC	1 cut(s)	753
MlsI	TGGCCA	1 cut(s)	1192
MluNI	TGGCCA	1 cut(s)	1192
MlyI	GAGTC	2 cut(s)	104, 257
Mox20I	TGGCCA	1 cut(s)	1192
Mph1103I	ATGCAT	1 cut(s)	709
MscI	TGGCCA	1 cut(s)	1192
MseI	TTAA	2 cut(s)	1182, 1428
MslI	CAYNNNNRTG	2 cut(s)	822, 999
Msp20I	TGGCCA	1 cut(s)	1192
MspI	CCGG	4 cut(s)	348, 504, 730, 989
MspR9I	CCNGG	8 cut(s)	263, 348, 349, 566, 579, 723, 990, 1408
MunI	CAATTG	1 cut(s)	152
MvaI	CCWGG	5 cut(s)	263, 566, 579, 723, 1408
MvnI	CGCG	2 cut(s)	79, 813
MwoI	GCNNNNNNNGC	2 cut(s)	16, 1455
NciI	CCSGG	3 cut(s)	348, 349, 990
NdeI	CATATG	1 cut(s)	1008
NdeII	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
NheI	GCTAGC	1 cut(s)	19
NlaIV	GGNNCC	4 cut(s)	346, 489, 720, 1164
NruI	TCGCGA	1 cut(s)	813
NsiI	ATGCAT	1 cut(s)	709
NspI	RCATGY	3 cut(s)	1157, 1204, 1462
PaeI	GCATGC	1 cut(s)	1462
PaeR7I	CTCGAG	1 cut(s)	32
PagI	TCATGA	1 cut(s)	742
PciSI	GCTCTTC	1 cut(s)	741
PcsI	WCGNNNNNNNCGW	3 cut(s)	83, 114, 182
PfeI	GAWTC	6 cut(s)	28, 37, 122, 413, 518, 1330
Pfl23II	CGTACG	1 cut(s)	114
PflMI	CCANNNNNTGG	1 cut(s)	268
PkrI	GCNGC	3 cut(s)	7, 292, 354
PleI	GAGTC	2 cut(s)	104, 256
PpsI	GAGTC	2 cut(s)	104, 256
Ppu21I	YACGTR	2 cut(s)	325, 1465
Psp124BI	GAGCTC	1 cut(s)	753
Psp6I	CCWGG	5 cut(s)	261, 564, 577, 721, 1406
PspFI	CCCAGC	1 cut(s)	1374
PspGI	CCWGG	5 cut(s)	261, 564, 577, 721, 1406
PspLI	CGTACG	1 cut(s)	114
PspN4I	GGNNCC	4 cut(s)	346, 489, 720, 1164
PspPI	GGNCC	6 cut(s)	344, 350, 488, 718, 1162, 1435
PstNI	CAGNNNCTG	1 cut(s)	268
RruI	TCGCGA	1 cut(s)	813
RsaI	GTAC	7 cut(s)	107, 116, 323, 572, 594, 1030, 1080
RsaNI	GTAC	7 cut(s)	106, 115, 322, 571, 593, 1029, 1079
RseI	CAYNNNNRTG	2 cut(s)	822, 999
SacI	GAGCTC	1 cut(s)	753
SapI	GCTCTTC	1 cut(s)	741
SaqAI	TTAA	2 cut(s)	1182, 1428
SatI	GCNGC	3 cut(s)	6, 291, 353
Sau3AI	GATC	7 cut(s)	127, 222, 478, 814, 1051, 1315, 1363
Sau96I	GGNCC	6 cut(s)	344, 350, 488, 718, 1162, 1435
ScaI	AGTACT	1 cut(s)	1030
SchI	GAGTC	2 cut(s)	104, 257
ScrFI	CCNGG	8 cut(s)	263, 348, 349, 566, 579, 723, 990, 1408
SduI	GDGCHC	1 cut(s)	753
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	4 cut(s)	694, 716, 847, 1349
Sfr274I	CTCGAG	1 cut(s)	32
SinI	GGWCC	4 cut(s)	344, 718, 1162, 1435
SlaI	CTCGAG	1 cut(s)	32
SmaI	CCCGGG	1 cut(s)	349
SmiMI	CAYNNNNRTG	2 cut(s)	822, 999
SmlI	CTYRAG	2 cut(s)	32, 1217
SmoI	CTYRAG	2 cut(s)	32, 1217
SphI	GCATGC	1 cut(s)	1462
SsiI	CCGC	4 cut(s)	83, 290, 353, 1225
SspMI	CTAG	2 cut(s)	20, 1026
SstI	GAGCTC	1 cut(s)	753
StyD4I	CCNGG	8 cut(s)	261, 346, 347, 564, 577, 721, 988, 1406
StyI	CCWWGG	1 cut(s)	1158
TaaI	ACNGT	3 cut(s)	210, 299, 1300
TaiI	ACGT	4 cut(s)	120, 327, 1422, 1467
TaqI	TCGA	9 cut(s)	33, 93, 120, 176, 185, 225, 240, 246, 530
TatI	WGTACW	2 cut(s)	1028, 1078
TauI	GCSGC	2 cut(s)	293, 355
TfiI	GAWTC	6 cut(s)	28, 37, 122, 413, 518, 1330
Tru1I	TTAA	2 cut(s)	1182, 1428
Tru9I	TTAA	2 cut(s)	1182, 1428
TscAI	CASTG	5 cut(s)	198, 481, 931, 1020, 1303
TseI	GCWGC	1 cut(s)	5
TspMI	CCCGGG	1 cut(s)	347
TspRI	CASTG	5 cut(s)	198, 481, 931, 1020, 1303
Van91I	CCANNNNNTGG	1 cut(s)	268
VpaK11BI	GGWCC	4 cut(s)	344, 718, 1162, 1435
XapI	RAATTY	6 cut(s)	56, 236, 403, 671, 737, 1133
XceI	RCATGY	3 cut(s)	1157, 1204, 1462
XhoI	CTCGAG	1 cut(s)	32
XmaI	CCCGGG	1 cut(s)	347
XspI	CTAG	2 cut(s)	20, 1026
ZrmI	AGTACT	1 cut(s)	1030
Zsp2I	ATGCAT	1 cut(s)	709

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.