MD13G1182700.v1.1

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002295
Physical Location & Seq
Reverse (-)
15307275 .. 15311782
4508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1182700.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGCATCCGATAAATGCGATCATGATGACTCGGATGCTGACTCGCAACTCGGGCCTCGAATCGGGGATGGGCGCCATCCCCTTCGGATCCGCCTGGTGGTTCATTTACGCCGGCCTCTCCTGTTTCTTCGTCATCTTCGCCGGTATAATGTCCGGCCTAACCCTAGGCCTCATGTCCCTCGGCCTCGTCGAGCTCGAGATTCTGCAGCGCAGCGGCTCCCCCACCGAGAAGAAACAAGCAGCTGTTATATTACCGGTGGTTCAGAAGCAGCATCAGCTTCTCGTCACTTTGCTACTGTGTAATGCGGCTGCTATGGAGGCGCTTCCGATATATTTGGATAAACTTTTCAATCAGTATGTTGCTATAATTCTCTCTGTGACCTTTGTCCTGTTTTTCGGAGAGGTTATTCCACAAGCAATATGTACTAGATACGGACTTGCTGTAGGTTCGAACTTTGTGTGGCTTGTGAGGATTTTAATGGTGGTTTGCTATCCAATTGCTTATCCAATTGGCAAGATCTTGGACTGTGTTCTTGGGCATAATGAAGCATTATTTAGGCGAGCTCAGTTAAAAGCCCTTGTTAACATCCACAGCCAGGAGGAGGGCAAGGGAGGTGAGCTTACACATGATGAGACGACGATAATTAGTGGAGCACTTGATTTAACTGAAAAGACAGCTGAGGAGGCTATGACGCCTATTGAATCAACATTTTCCTTAGATGTCAATTCAAAATTGGACTGGGAAGCAATGGGGAAAGTTCTTGCTCGGGGACATAGTCGAGTTCCTGTCTATTCTGGGAACCCAAAGAATATTATTGGACTTCTGCTGGTGAAAAGTCTTCTCACTGTACGACCTGAAACGGAGACCCCAGTTAGTGCTGTTTCCATTCGTAGAATTCCACGAGTTCCTGCAGATATGCCACTGTATGATATACTGAACGAGTTTCAAAAGGGCAGCAGTCATATGGCTGCTGTGGTGAAGCCTAAAACGAAAAGTAAGGCTCCTCTACCAACCATTGACGGCAAGAAAGATGAAGGGAACGGAGACACTGGCACAGACTCCCAACTGACTACTCCTTTGCTATCCAAGCAGGATGGAAAGCCGGACGGTGTTGTTGTTGATATACCCAGGCTTCCGAGGCCCCATAGTCATAGACACAAGGAAACTTTTTCATCCCATAGTGATGGAGCAACAAATGGATTGCCCCAGGTATCAGAGGATATTGAGGAGGGTGAAGTCATTGGGATTATCACTCTGGAAGATGTATTTGAAGAACTCCTGCAGGAGGAGATTGTGGATGAAACAGATGAATATGTTGATGTACATAAAAGAATACGTGTCGCTGCAGCTGCAGCTGCTTCATCAGTGGCACGGGCTCCATCGCTTCGGAGGTTAAATGTGAACAAGGGACCTGCCACTGGACAAAATGTAGGCCAAAAAGTAAGCAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

485

Amino Acids

52.69

Weight (kDa)

5.99

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 40 - 209 2.1e-34 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1420
AccB1I GGYRCC 1 cut(s) 71
AciI CCGC 3 cut(s) 90, 213, 305
AclWI GGATC 2 cut(s) 81, 94
AcsI RAATTY 1 cut(s) 894
AcyI GRCGYC 2 cut(s) 72, 692
AfaI GTAC 3 cut(s) 424, 849, 1323
AfiI CCNNNNNNNGG 6 cut(s) 61, 96, 595, 601, 1285, 1418
AflIII ACRYGT 1 cut(s) 1336
AgeI ACCGGT 1 cut(s) 253
AgsI TTSAA 5 cut(s) 349, 701, 729, 947, 1271
AjnI CCWGG 4 cut(s) 92, 594, 1127, 1206
AluBI AGCT 8 cut(s) 193, 242, 277, 563, 619, 677, 1349, 1355
AluI AGCT 8 cut(s) 193, 242, 277, 563, 619, 677, 1349, 1355
Alw21I GWGCWC 3 cut(s) 195, 565, 655
Alw26I GTCTC 3 cut(s) 626, 857, 1038
AlwI GGATC 2 cut(s) 81, 94
Ama87I CYCGRG 3 cut(s) 49, 194, 765
AoxI GGCC 7 cut(s) 52, 112, 154, 166, 181, 1139, 1432
ApoI RAATTY 1 cut(s) 894
ArsI GACNNNNNNTTYG 2 cut(s) 1060, 1092
AsiGI ACCGGT 1 cut(s) 253
AspA2I CCTAGG 1 cut(s) 163
AspLEI GCGC 3 cut(s) 74, 210, 322
AspS9I GGNCC 3 cut(s) 52, 1140, 1409
AsuHPI GGTGA 4 cut(s) 626, 841, 988, 1244
AsuII TTCGAA 1 cut(s) 449
AvaI CYCGRG 3 cut(s) 49, 194, 765
AvaII GGWCC 1 cut(s) 1409
AvrII CCTAGG 1 cut(s) 163
BamHI GGATCC 1 cut(s) 86
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 3 cut(s) 195, 565, 1378
BauI CACGAG 1 cut(s) 900
BbsI GAAGAC 1 cut(s) 830
Bbv12I GWGCWC 3 cut(s) 195, 565, 655
BbvCI CCTCAGC 1 cut(s) 678
BccI CCATC 5 cut(s) 61, 83, 1088, 1178, 1387
BceAI ACGGC 1 cut(s) 1036
BciT130I CCWGG 4 cut(s) 94, 596, 1129, 1208
BcoDI GTCTC 3 cut(s) 626, 857, 1038
BfaI CTAG 2 cut(s) 164, 426
BfmI CTRYAG 6 cut(s) 203, 441, 909, 1280, 1344, 1350
BfoI RGCGCY 2 cut(s) 75, 323
BfuAI ACCTGC 1 cut(s) 1420
BglII AGATCT 1 cut(s) 516
BlnI CCTAGG 1 cut(s) 163
Bme1390I CCNGG 4 cut(s) 94, 596, 1129, 1208
Bme18I GGWCC 1 cut(s) 1409
BmeT110I CYCGRG 3 cut(s) 49, 194, 765
BmgT120I GGNCC 3 cut(s) 52, 1140, 1409
BmiI GGNNCC 8 cut(s) 73, 88, 217, 800, 1002, 1142, 1377, 1410
BmrFI CCNGG 4 cut(s) 94, 596, 1129, 1208
BmrI ACTGGG 2 cut(s) 748, 863
BmsI GCATC 3 cut(s) 13, 24, 280
BmuI ACTGGG 2 cut(s) 748, 863
BoxI GACNNNNGTC 1 cut(s) 383
BpiI GAAGAC 1 cut(s) 830
Bpu10I CCTNAGC 1 cut(s) 678
Bpu14I TTCGAA 1 cut(s) 449
BsaAI YACGTR 1 cut(s) 1337
BsaHI GRCGYC 2 cut(s) 72, 692
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 5 cut(s) 163, 178, 1127, 1136, 1206
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 96, 595, 601, 1285, 1418
Bse118I RCCGGY 3 cut(s) 110, 140, 253
Bse1I ACTGG 4 cut(s) 743, 869, 1054, 1423
Bse3DI GCAATG 1 cut(s) 753
BseBI CCWGG 4 cut(s) 94, 596, 1129, 1208
BseDI CCNNGG 5 cut(s) 163, 178, 1127, 1136, 1206
BseGI GGATG 8 cut(s) 4, 39, 72, 75, 585, 1099, 1172, 1303
BseLI CCNNNNNNNGG 6 cut(s) 61, 96, 595, 601, 1285, 1418
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 2 cut(s) 578, 669
BseNI ACTGG 4 cut(s) 743, 869, 1054, 1423
BseRI GAGGAG 5 cut(s) 614, 695, 993, 1241, 1301
BshFI GGCC 7 cut(s) 54, 114, 156, 168, 183, 1141, 1434
BshNI GGYRCC 1 cut(s) 71
BshTI ACCGGT 1 cut(s) 253
BsiHKAI GWGCWC 3 cut(s) 195, 565, 655
BsiHKCI CYCGRG 3 cut(s) 49, 194, 765
BsiSI CCGG 5 cut(s) 111, 141, 153, 254, 1103
BslFI GGGAC 3 cut(s) 160, 783, 1422
BslI CCNNNNNNNGG 6 cut(s) 61, 96, 595, 601, 1285, 1418
BsmAI GTCTC 3 cut(s) 626, 857, 1038
BsmBI CGTCTC 1 cut(s) 626
BsmFI GGGAC 3 cut(s) 160, 783, 1422
BsnI GGCC 7 cut(s) 54, 114, 156, 168, 183, 1141, 1434
Bso31I GGTCTC 1 cut(s) 857
BsoBI CYCGRG 3 cut(s) 49, 194, 765
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 4 cut(s) 195, 565, 655, 1378
Bsp1407I TGTACA 1 cut(s) 1321
Bsp143I GATC 3 cut(s) 18, 86, 516
BspACI CCGC 3 cut(s) 90, 213, 305
BspANI GGCC 7 cut(s) 54, 114, 156, 168, 183, 1141, 1434
BspCNI CTCAG 2 cut(s) 577, 670
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 8 cut(s) 73, 88, 217, 800, 1002, 1142, 1377, 1410
BspMAI CTGCAG 5 cut(s) 207, 913, 1284, 1348, 1354
BspMI ACCTGC 1 cut(s) 1420
BspPI GGATC 2 cut(s) 81, 94
BspT104I TTCGAA 1 cut(s) 449
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 857
BsrDI GCAATG 1 cut(s) 753
BsrFI RCCGGY 3 cut(s) 110, 140, 253
BsrGI TGTACA 1 cut(s) 1321
BsrI ACTGG 4 cut(s) 743, 869, 1054, 1423
BssAI RCCGGY 3 cut(s) 110, 140, 253
BssECI CCNNGG 5 cut(s) 163, 178, 1127, 1136, 1206
BssMI GATC 3 cut(s) 18, 86, 516
BssNI GRCGYC 2 cut(s) 72, 692
BssSI CACGAG 1 cut(s) 900
BssT1I CCWWGG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 900
Bst2UI CCWGG 4 cut(s) 94, 596, 1129, 1208
Bst4CI ACNGT 5 cut(s) 297, 527, 847, 924, 1109
BstACI GRCGYC 2 cut(s) 72, 692
BstAUI TGTACA 1 cut(s) 1321
BstBAI YACGTR 1 cut(s) 1337
BstBI TTCGAA 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 112, 561
BstDEI CTNAG 3 cut(s) 564, 678, 715
BstENI CCTNNNNNAGG 1 cut(s) 1283
BstF5I GGATG 8 cut(s) 4, 39, 72, 75, 585, 1099, 1172, 1303
BstH2I RGCGCY 2 cut(s) 75, 323
BstHHI GCGC 3 cut(s) 74, 210, 322
BstKTI GATC 3 cut(s) 21, 89, 519
BstMAI GTCTC 3 cut(s) 626, 857, 1038
BstMBI GATC 3 cut(s) 18, 86, 516
BstMWI GCNNNNNNNGC 9 cut(s) 51, 274, 317, 683, 1087, 1138, 1349, 1352, 1355
BstNI CCWGG 4 cut(s) 94, 596, 1129, 1208
BstPAI GACNNNNGTC 1 cut(s) 383
BstSCI CCNGG 4 cut(s) 92, 594, 1127, 1206
BstSFI CTRYAG 6 cut(s) 203, 441, 909, 1280, 1344, 1350
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 2 cut(s) 86, 516
BstXI CCANNNNNNTGG 1 cut(s) 1184
BstYI RGATCY 2 cut(s) 86, 516
BsuRI GGCC 7 cut(s) 54, 114, 156, 168, 183, 1141, 1434
BtgZI GCGATG 1 cut(s) 1365
BtsCI GGATG 8 cut(s) 4, 39, 72, 75, 585, 1099, 1172, 1303
BtsIMutI CAGTG 5 cut(s) 843, 920, 1047, 1371, 1416
BveI ACCTGC 1 cut(s) 1420
Cac8I GCNNGC 2 cut(s) 112, 561
CciI TCATGA 1 cut(s) 21
CfoI GCGC 3 cut(s) 74, 210, 322
Cfr10I RCCGGY 3 cut(s) 110, 140, 253
Cfr13I GGNCC 3 cut(s) 52, 1140, 1409
CseI GACGC 1 cut(s) 700
Csp6I GTAC 3 cut(s) 423, 848, 1322
CspAI ACCGGT 1 cut(s) 253
CviAII CATG 3 cut(s) 22, 172, 626
CviQI GTAC 3 cut(s) 423, 848, 1322
DdeI CTNAG 3 cut(s) 564, 678, 715
DinI GGCGCC 1 cut(s) 73
DpnI GATC 3 cut(s) 20, 88, 518
DpnII GATC 3 cut(s) 18, 86, 516
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 2 cut(s) 193, 563
Eco130I CCWWGG 1 cut(s) 163
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 3 cut(s) 195, 565, 1378
Eco31I GGTCTC 1 cut(s) 857
Eco47I GGWCC 1 cut(s) 1409
Eco53kI GAGCTC 2 cut(s) 193, 563
Eco88I CYCGRG 3 cut(s) 49, 194, 765
EcoICRI GAGCTC 2 cut(s) 193, 563
EcoNI CCTNNNNNAGG 1 cut(s) 1283
EcoO109I RGGNCCY 2 cut(s) 1140, 1409
EcoRI GAATTC 1 cut(s) 894
EcoRII CCWGG 4 cut(s) 92, 594, 1127, 1206
EcoT14I CCWWGG 1 cut(s) 163
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 195, 565, 1378
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 163
Esp3I CGTCTC 1 cut(s) 626
FaeI CATG 3 cut(s) 25, 175, 629
FaqI GGGAC 3 cut(s) 160, 783, 1422
FatI CATG 3 cut(s) 21, 171, 625
FauNDI CATATG 1 cut(s) 963
FokI GGATG 7 cut(s) 46, 62, 79, 572, 1106, 1159, 1310
FriOI GRGCYC 3 cut(s) 195, 565, 1378
FspBI CTAG 2 cut(s) 164, 426
GlaI GCGC 3 cut(s) 73, 209, 321
HaeII RGCGCY 2 cut(s) 75, 323
HaeIII GGCC 7 cut(s) 54, 114, 156, 168, 183, 1141, 1434
HapII CCGG 5 cut(s) 111, 141, 153, 254, 1103
HgaI GACGC 1 cut(s) 700
HhaI GCGC 3 cut(s) 74, 210, 322
Hin1I GRCGYC 2 cut(s) 72, 692
Hin1II CATG 3 cut(s) 25, 175, 629
Hin6I GCGC 3 cut(s) 72, 208, 320
HinP1I GCGC 3 cut(s) 72, 208, 320
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HinfI GANTC 6 cut(s) 28, 40, 59, 199, 701, 1058
HpaI GTTAAC 1 cut(s) 583
HpaII CCGG 5 cut(s) 111, 141, 153, 254, 1103
HphI GGTGA 4 cut(s) 626, 841, 988, 1244
Hpy166II GTNNAC 2 cut(s) 583, 1402
Hpy188I TCNGA 9 cut(s) 9, 33, 86, 264, 327, 398, 1137, 1216, 1389
Hpy188III TCNNGA 3 cut(s) 22, 196, 1256
Hpy8I GTNNAC 2 cut(s) 583, 1402
Hpy99I CGWCG 2 cut(s) 191, 640
HpyAV CCTTC 2 cut(s) 91, 1028
HpyCH4III ACNGT 5 cut(s) 297, 527, 847, 924, 1109
HpyCH4IV ACGT 1 cut(s) 1336
HpyCH4V TGCA 6 cut(s) 4, 205, 911, 1282, 1346, 1352
HpyF10VI GCNNNNNNNGC 9 cut(s) 51, 274, 317, 683, 1087, 1138, 1349, 1352, 1355
HpyF3I CTNAG 3 cut(s) 564, 678, 715
HpySE526I ACGT 1 cut(s) 1336
Hsp92I GRCGYC 2 cut(s) 72, 692
Hsp92II CATG 3 cut(s) 25, 175, 629
HspAI GCGC 3 cut(s) 72, 208, 320
KasI GGCGCC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
KspAI GTTAAC 1 cut(s) 583
Kzo9I GATC 3 cut(s) 18, 86, 516
LmnI GCTCC 5 cut(s) 221, 650, 1006, 1187, 1381
LweI GCATC 3 cut(s) 13, 24, 280
MaeI CTAG 2 cut(s) 164, 426
MaeII ACGT 1 cut(s) 1336
MaeIII GTNAC 2 cut(s) 283, 376
MalI GATC 3 cut(s) 20, 88, 518
MboI GATC 3 cut(s) 18, 86, 516
MboII GAAGA 6 cut(s) 118, 127, 241, 830, 1271, 1283
MfeI CAATTG 2 cut(s) 495, 507
MflI RGATCY 2 cut(s) 86, 516
MhlI GDGCHC 4 cut(s) 195, 565, 655, 1378
MluCI AATT 7 cut(s) 366, 495, 507, 642, 724, 731, 894
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 22, 34, 1052
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 110
MseI TTAA 5 cut(s) 476, 569, 582, 662, 1394
MslI CAYNNNNRTG 1 cut(s) 1182
MspA1I CMGCKG 5 cut(s) 213, 242, 677, 1349, 1355
MspI CCGG 5 cut(s) 111, 141, 153, 254, 1103
MspR9I CCNGG 4 cut(s) 94, 596, 1129, 1208
MunI CAATTG 2 cut(s) 495, 507
MvaI CCWGG 4 cut(s) 94, 596, 1129, 1208
MwoI GCNNNNNNNGC 9 cut(s) 51, 274, 317, 683, 1087, 1138, 1349, 1352, 1355
NaeI GCCGGC 1 cut(s) 112
NarI GGCGCC 1 cut(s) 72
NdeI CATATG 1 cut(s) 963
NdeII GATC 3 cut(s) 18, 86, 516
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 3 cut(s) 25, 175, 629
NlaIV GGNNCC 8 cut(s) 73, 88, 217, 800, 1002, 1142, 1377, 1410
NmeAIII GCCGAG 1 cut(s) 159
NmuCI GTSAC 2 cut(s) 283, 376
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 449
PaeR7I CTCGAG 1 cut(s) 194
PagI TCATGA 1 cut(s) 21
PceI AGGCCT 1 cut(s) 168
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 192
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 3 cut(s) 59, 199, 701
PflFI GACNNNGTC 1 cut(s) 774
PinAI ACCGGT 1 cut(s) 253
PleI GAGTC 3 cut(s) 22, 34, 1052
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 22, 34, 1052
Ppu21I YACGTR 1 cut(s) 1337
PpuMI RGGWCCY 1 cut(s) 1409
PshAI GACNNNNGTC 1 cut(s) 383
Psp124BI GAGCTC 2 cut(s) 195, 565
Psp5II RGGWCCY 1 cut(s) 1409
Psp6I CCWGG 4 cut(s) 92, 594, 1127, 1206
PspGI CCWGG 4 cut(s) 92, 594, 1127, 1206
PspN4I GGNNCC 8 cut(s) 73, 88, 217, 800, 1002, 1142, 1377, 1410
PspPI GGNCC 3 cut(s) 52, 1140, 1409
PspPPI RGGWCCY 1 cut(s) 1409
PstI CTGCAG 5 cut(s) 207, 913, 1284, 1348, 1354
PsuI RGATCY 2 cut(s) 86, 516
PsyI GACNNNGTC 1 cut(s) 774
PvuII CAGCTG 4 cut(s) 242, 677, 1349, 1355
RsaI GTAC 3 cut(s) 424, 849, 1323
RsaNI GTAC 3 cut(s) 423, 848, 1322
RseI CAYNNNNRTG 1 cut(s) 1182
SacI GAGCTC 2 cut(s) 195, 565
SaqAI TTAA 5 cut(s) 476, 569, 582, 662, 1394
Sau3AI GATC 3 cut(s) 18, 86, 516
Sau96I GGNCC 3 cut(s) 52, 1140, 1409
SbfI CCTGCAGG 1 cut(s) 1284
SchI GAGTC 3 cut(s) 22, 34, 1052
ScrFI CCNGG 4 cut(s) 94, 596, 1129, 1208
SdaI CCTGCAGG 1 cut(s) 1284
SduI GDGCHC 4 cut(s) 195, 565, 655, 1378
SfaNI GCATC 3 cut(s) 13, 24, 280
SfcI CTRYAG 6 cut(s) 203, 441, 909, 1280, 1344, 1350
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 194
SfuI TTCGAA 1 cut(s) 449
SinI GGWCC 1 cut(s) 1409
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 1182
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse8387I CCTGCAGG 1 cut(s) 1284
Sse9I AATT 7 cut(s) 366, 495, 507, 642, 724, 731, 894
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 3 cut(s) 90, 213, 305
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 811
SspMI CTAG 2 cut(s) 164, 426
SstI GAGCTC 2 cut(s) 195, 565
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 92, 594, 1127, 1206
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 5 cut(s) 297, 527, 847, 924, 1109
TaiI ACGT 1 cut(s) 1339
TaqI TCGA 5 cut(s) 57, 189, 195, 449, 778
TasI AATT 7 cut(s) 366, 495, 507, 642, 724, 731, 894
TatI WGTACW 2 cut(s) 422, 1321
TauI GCSGC 2 cut(s) 216, 308
TfiI GAWTC 3 cut(s) 59, 199, 701
Tru1I TTAA 5 cut(s) 476, 569, 582, 662, 1394
Tru9I TTAA 5 cut(s) 476, 569, 582, 662, 1394
TscAI CASTG 5 cut(s) 850, 927, 1054, 1371, 1423
TseFI GTSAC 2 cut(s) 283, 376
Tsp45I GTSAC 2 cut(s) 283, 376
TspDTI ATGAA 7 cut(s) 91, 558, 1047, 1161, 1314, 1323, 1350
TspGWI ACGGA 3 cut(s) 447, 875, 1056
TspRI CASTG 5 cut(s) 850, 927, 1054, 1371, 1423
Tth111I GACNNNGTC 1 cut(s) 774
VpaK11BI GGWCC 1 cut(s) 1409
XagI CCTNNNNNAGG 1 cut(s) 1283
XapI RAATTY 1 cut(s) 894
XhoI CTCGAG 1 cut(s) 194
XmaJI CCTAGG 1 cut(s) 163
XspI CTAG 2 cut(s) 164, 426
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.