Rh4DG213100

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002295
Physical Location & Seq
Forward (+)
40950303 .. 40954097
3795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG213100.1

Sequence Viewer

Length: 1155 bp
ATGCATCCCCTCAATGCAGTCATGATGACCCGGATGCTGGTTCGGAACAACAACAACAACAACTCCGGTCTGGAATCCGGGGGCATAGCCTTCGCATCGATTTGGTGGTTTGTCTACATCGGCTTGTCGTGTTTCTTGGTCCTCTTCGCTGGAATAATGTCAGGCTTAACTTTAGGACTCATGTCCCTCGGCCTCGTCGACCTCGAGATTCTACAGCGAAGTGGTACTCCCACCGAGAAGAAGCAAGCAGCGACTATACTTCCGGTGGTTCAGAAGCAGCATCAGCTGCTCGTCACTTTGCTTCTGTGTAATGCTGGAGCTATGGAAACTGCTGAGGAGGCTATGACACCTATTGAATCAACCTTCTCCTTAGATGTCAATTCAAAGTTGGACTGGGAAGCAATGGGGAAAGTTCTTGCTCGGGGACATAGTCGAGTTCCTGTCTATTCTGGGAATTCAAGGAATATTATTGGACTTCTCCTGGTGAAAAGTCTTCTCACTGTACGACCCGAAACAGAGACCCCTGTCAGTGCTGTTTCCATCCGTAGAATCCCAAGAGTTCCCGCAGATATGCCACTGTATGATATATTGAATGAGTTTCAAAAGGGCAGCAGTCATATGGCTGCTGTGGTAAAGGCCAGAGGGAAAAGTAAGACTCATCCACCCACGATTGACGGAGAAAATAATGAAGGAAACAAAATTGCTGGCATGGACTCCCAACTGACTACTCCATTGCTATCCAAGCAGGATGGAAAGCCAGACCATGTTGTTGTTGACGTGCCCAAGATACCAAAGCCCACTAGTATGAAAAAGGAAGCACTCTCGCACTACAATGTTGGAGAAACAATTGGCCCCCCTTCGTCAGAGGATATTGAGGATGGTGAAGTCATTGGTATCATCACCCTGGAAGATGTATTTGAAGAACTCCTGCAGGAGGAGATTGTCGATGAAACGGATGAATATGTTGATGTACATAAAAGAATACGCGTTGCTGCAGCTGCAGCTGCTTCATCAGTGGCGCGAGCTCCATCACTGCGGAGGTTAAATGTCAACAAGGGACCAGGAGCCCAAACTAAGCAAGTAACCCCGAAGAAATCTACTGATGAATTGGCTGCAAGGCCACACGGGACTTTTGCTGATGGAAACAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

384

Amino Acids

41.64

Weight (kDa)

6.33

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 42 - 109 2.6e-09 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 114, 198
AccII CGCG 2 cut(s) 987, 1021
AciI CCGC 2 cut(s) 564, 1036
AcsI RAATTY 1 cut(s) 454
AfaI GTAC 3 cut(s) 226, 504, 972
AfiI CCNNNNNNNGG 2 cut(s) 37, 934
AflIII ACRYGT 1 cut(s) 985
AgsI TTSAA 6 cut(s) 356, 384, 459, 592, 602, 920
AhlI ACTAGT 1 cut(s) 800
AjiI CACGTC 1 cut(s) 778
AjnI CCWGG 3 cut(s) 480, 903, 1060
AluBI AGCT 5 cut(s) 286, 320, 998, 1004, 1025
AluI AGCT 5 cut(s) 286, 320, 998, 1004, 1025
Alw21I GWGCWC 1 cut(s) 1027
Alw26I GTCTC 1 cut(s) 512
Ama87I CYCGRG 2 cut(s) 203, 420
AoxI GGCC 4 cut(s) 190, 636, 850, 1118
ApoI RAATTY 1 cut(s) 454
AspLEI GCGC 1 cut(s) 1021
AspS9I GGNCC 3 cut(s) 139, 851, 1058
AsuC2I CCSGG 2 cut(s) 31, 79
AsuHPI GGTGA 3 cut(s) 496, 892, 893
AvaI CYCGRG 2 cut(s) 203, 420
AvaII GGWCC 2 cut(s) 139, 1058
BaeGI GKGCMC 1 cut(s) 783
BanII GRGCYC 2 cut(s) 1027, 1069
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 1027
BbvCI CCTCAGC 1 cut(s) 333
BccI CCATC 5 cut(s) 548, 743, 872, 1036, 1133
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 3 cut(s) 482, 905, 1062
BcnI CCSGG 2 cut(s) 31, 79
BcoDI GTCTC 1 cut(s) 512
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 1 cut(s) 801
BfmI CTRYAG 4 cut(s) 212, 929, 993, 999
Bme1390I CCNGG 5 cut(s) 31, 79, 482, 905, 1062
Bme18I GGWCC 2 cut(s) 139, 1058
BmeT110I CYCGRG 2 cut(s) 203, 420
BmgBI CACGTC 1 cut(s) 778
BmgT120I GGNCC 3 cut(s) 139, 851, 1058
BmiI GGNNCC 3 cut(s) 853, 1059, 1066
BmrFI CCNGG 5 cut(s) 31, 79, 482, 905, 1062
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 4 cut(s) 13, 24, 104, 289
BmuI ACTGGG 1 cut(s) 403
BoxI GACNNNNGTC 2 cut(s) 181, 524
BpiI GAAGAC 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 333
BpuMI CCSGG 2 cut(s) 31, 79
Bsa29I ATCGAT 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 3 cut(s) 78, 187, 903
BsaWI WCCGGW 2 cut(s) 65, 262
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 934
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 2 cut(s) 408, 731
BseBI CCWGG 3 cut(s) 482, 905, 1062
BseCI ATCGAT 1 cut(s) 98
BseDI CCNNGG 3 cut(s) 78, 187, 903
BseGI GGATG 7 cut(s) 4, 39, 540, 658, 754, 883, 961
BseLI CCNNNNNNNGG 2 cut(s) 37, 934
BseMI GCAATG 2 cut(s) 408, 731
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 2 cut(s) 350, 950
BseSI GKGCMC 1 cut(s) 783
Bsh1236I CGCG 2 cut(s) 987, 1021
BshFI GGCC 4 cut(s) 192, 638, 852, 1120
BshVI ATCGAT 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 1027
BsiHKCI CYCGRG 2 cut(s) 203, 420
BsiSI CCGG 4 cut(s) 31, 66, 78, 263
BslFI GGGAC 4 cut(s) 169, 438, 1071, 1141
BslI CCNNNNNNNGG 2 cut(s) 37, 934
BsmAI GTCTC 1 cut(s) 512
BsmFI GGGAC 4 cut(s) 169, 438, 1071, 1141
BsnI GGCC 4 cut(s) 192, 638, 852, 1120
Bso31I GGTCTC 1 cut(s) 512
BsoBI CYCGRG 2 cut(s) 203, 420
Bsp1286I GDGCHC 3 cut(s) 783, 1027, 1069
Bsp1407I TGTACA 1 cut(s) 970
BspACI CCGC 2 cut(s) 564, 1036
BspANI GGCC 4 cut(s) 192, 638, 852, 1120
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 98
BspFNI CGCG 2 cut(s) 987, 1021
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 853, 1059, 1066
BspMAI CTGCAG 3 cut(s) 933, 997, 1003
BspTNI GGTCTC 1 cut(s) 512
BsrDI GCAATG 2 cut(s) 408, 731
BsrGI TGTACA 1 cut(s) 970
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 78, 187, 903
Bst2UI CCWGG 3 cut(s) 482, 905, 1062
Bst4CI ACNGT 2 cut(s) 502, 579
Bst6I CTCTTC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 286
BstAUI TGTACA 1 cut(s) 970
BstC8I GCNNGC 3 cut(s) 246, 706, 1023
BstDEI CTNAG 3 cut(s) 333, 370, 1074
BstENI CCTNNNNNAGG 1 cut(s) 932
BstF5I GGATG 7 cut(s) 4, 39, 540, 658, 754, 883, 961
BstFNI CGCG 2 cut(s) 987, 1021
BstHHI GCGC 1 cut(s) 1021
BstMAI GTCTC 1 cut(s) 512
BstMWI GCNNNNNNNGC 7 cut(s) 283, 286, 338, 742, 998, 1001, 1004
BstNI CCWGG 3 cut(s) 482, 905, 1062
BstPAI GACNNNNGTC 2 cut(s) 181, 524
BstSCI CCNGG 5 cut(s) 29, 77, 480, 903, 1060
BstSFI CTRYAG 4 cut(s) 212, 929, 993, 999
BstSLI GKGCMC 1 cut(s) 783
BstUI CGCG 2 cut(s) 987, 1021
BstV2I GAAGAC 1 cut(s) 485
Bsu15I ATCGAT 1 cut(s) 98
BsuRI GGCC 4 cut(s) 192, 638, 852, 1120
BsuTUI ATCGAT 1 cut(s) 98
BtrI CACGTC 1 cut(s) 778
BtsCI GGATG 7 cut(s) 4, 39, 540, 658, 754, 883, 961
BtsI GCAGTG 1 cut(s) 1031
BtsIMutI CAGTG 5 cut(s) 498, 535, 575, 1020, 1031
Cac8I GCNNGC 3 cut(s) 246, 706, 1023
CciI TCATGA 1 cut(s) 21
CfoI GCGC 1 cut(s) 1021
Cfr13I GGNCC 3 cut(s) 139, 851, 1058
ClaI ATCGAT 1 cut(s) 98
Csp6I GTAC 3 cut(s) 225, 503, 971
CviAII CATG 4 cut(s) 22, 181, 709, 764
CviQI GTAC 3 cut(s) 225, 503, 971
DdeI CTNAG 3 cut(s) 333, 370, 1074
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 1025
Eco24I GRGCYC 2 cut(s) 1027, 1069
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 2 cut(s) 139, 1058
Eco53kI GAGCTC 1 cut(s) 1025
Eco88I CYCGRG 2 cut(s) 203, 420
EcoICRI GAGCTC 1 cut(s) 1025
EcoNI CCTNNNNNAGG 1 cut(s) 932
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 3 cut(s) 480, 903, 1060
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1027, 1069
FaeI CATG 4 cut(s) 25, 184, 712, 767
FaqI GGGAC 4 cut(s) 169, 438, 1071, 1141
FatI CATG 4 cut(s) 21, 180, 708, 763
FauI CCCGC 1 cut(s) 571
FauNDI CATATG 1 cut(s) 618
FblI GTMKAC 2 cut(s) 114, 198
FokI GGATG 6 cut(s) 46, 527, 645, 761, 890, 968
FriOI GRGCYC 2 cut(s) 1027, 1069
FspBI CTAG 1 cut(s) 801
GlaI GCGC 1 cut(s) 1020
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 4 cut(s) 192, 638, 852, 1120
HapII CCGG 4 cut(s) 31, 66, 78, 263
HhaI GCGC 1 cut(s) 1021
Hin1II CATG 4 cut(s) 25, 184, 712, 767
Hin6I GCGC 1 cut(s) 1019
HinP1I GCGC 1 cut(s) 1019
HincII GTYRAC 3 cut(s) 199, 775, 1051
HindII GTYRAC 3 cut(s) 199, 775, 1051
HinfI GANTC 7 cut(s) 74, 177, 208, 356, 549, 655, 713
HpaII CCGG 4 cut(s) 31, 66, 78, 263
HphI GGTGA 3 cut(s) 496, 892, 893
Hpy166II GTNNAC 4 cut(s) 115, 199, 775, 1051
Hpy188I TCNGA 3 cut(s) 45, 273, 865
Hpy188III TCNNGA 3 cut(s) 22, 71, 205
Hpy8I GTNNAC 4 cut(s) 115, 199, 775, 1051
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 4 cut(s) 100, 373, 683, 867
HpyCH4III ACNGT 2 cut(s) 502, 579
HpyCH4IV ACGT 1 cut(s) 777
HpyCH4V TGCA 6 cut(s) 4, 17, 931, 995, 1001, 1115
HpyF10VI GCNNNNNNNGC 7 cut(s) 283, 286, 338, 742, 998, 1001, 1004
HpyF3I CTNAG 3 cut(s) 333, 370, 1074
HpySE526I ACGT 1 cut(s) 777
Hsp92II CATG 4 cut(s) 25, 184, 712, 767
HspAI GCGC 1 cut(s) 1019
LmnI GCTCC 3 cut(s) 317, 1030, 1064
LweI GCATC 4 cut(s) 13, 24, 104, 289
MaeI CTAG 1 cut(s) 801
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 2 cut(s) 292, 1081
MboII GAAGA 6 cut(s) 136, 250, 485, 920, 932, 1102
MfeI CAATTG 1 cut(s) 846
MhlI GDGCHC 3 cut(s) 783, 1027, 1069
MluCI AATT 5 cut(s) 379, 454, 699, 846, 1106
MluI ACGCGT 1 cut(s) 985
MlyI GAGTC 3 cut(s) 171, 649, 707
MmeI TCCRAC 2 cut(s) 369, 817
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 167, 1043
MslI CAYNNNNRTG 2 cut(s) 803, 831
MspA1I CMGCKG 3 cut(s) 286, 998, 1004
MspI CCGG 4 cut(s) 31, 66, 78, 263
MspR9I CCNGG 5 cut(s) 31, 79, 482, 905, 1062
MunI CAATTG 1 cut(s) 846
MvaI CCWGG 3 cut(s) 482, 905, 1062
MvnI CGCG 2 cut(s) 987, 1021
MwoI GCNNNNNNNGC 7 cut(s) 283, 286, 338, 742, 998, 1001, 1004
NciI CCSGG 2 cut(s) 31, 79
NdeI CATATG 1 cut(s) 618
NlaIII CATG 4 cut(s) 25, 184, 712, 767
NlaIV GGNNCC 3 cut(s) 853, 1059, 1066
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 201
PfeI GAWTC 4 cut(s) 74, 208, 356, 549
PflFI GACNNNGTC 1 cut(s) 429
PleI GAGTC 3 cut(s) 171, 649, 707
PpsI GAGTC 3 cut(s) 171, 649, 707
PshAI GACNNNNGTC 2 cut(s) 181, 524
Psp124BI GAGCTC 1 cut(s) 1027
Psp6I CCWGG 3 cut(s) 480, 903, 1060
PspGI CCWGG 3 cut(s) 480, 903, 1060
PspN4I GGNNCC 3 cut(s) 853, 1059, 1066
PspPI GGNCC 3 cut(s) 139, 851, 1058
PstI CTGCAG 3 cut(s) 933, 997, 1003
PsyI GACNNNGTC 1 cut(s) 429
PvuII CAGCTG 3 cut(s) 286, 998, 1004
RsaI GTAC 3 cut(s) 226, 504, 972
RsaNI GTAC 3 cut(s) 225, 503, 971
RseI CAYNNNNRTG 2 cut(s) 803, 831
SacI GAGCTC 1 cut(s) 1027
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 167, 1043
Sau96I GGNCC 3 cut(s) 139, 851, 1058
SbfI CCTGCAGG 1 cut(s) 933
SchI GAGTC 3 cut(s) 171, 649, 707
ScrFI CCNGG 5 cut(s) 31, 79, 482, 905, 1062
SdaI CCTGCAGG 1 cut(s) 933
SduI GDGCHC 3 cut(s) 783, 1027, 1069
SfaNI GCATC 4 cut(s) 13, 24, 104, 289
SfcI CTRYAG 4 cut(s) 212, 929, 993, 999
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 2 cut(s) 139, 1058
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 2 cut(s) 803, 831
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SpeI ACTAGT 1 cut(s) 800
Sse8387I CCTGCAGG 1 cut(s) 933
Sse9I AATT 5 cut(s) 379, 454, 699, 846, 1106
SsiI CCGC 2 cut(s) 564, 1036
SspI AATATT 1 cut(s) 466
SspMI CTAG 1 cut(s) 801
SstI GAGCTC 1 cut(s) 1027
StyD4I CCNGG 5 cut(s) 29, 77, 480, 903, 1060
TaaI ACNGT 2 cut(s) 502, 579
TaiI ACGT 1 cut(s) 780
TaqI TCGA 5 cut(s) 98, 198, 204, 433, 945
TasI AATT 5 cut(s) 379, 454, 699, 846, 1106
TatI WGTACW 1 cut(s) 970
TfiI GAWTC 4 cut(s) 74, 208, 356, 549
Tru1I TTAA 2 cut(s) 167, 1043
Tru9I TTAA 2 cut(s) 167, 1043
TscAI CASTG 5 cut(s) 505, 535, 582, 1020, 1038
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 6 cut(s) 702, 821, 963, 972, 999, 1119
TspGWI ACGGA 3 cut(s) 533, 690, 968
TspRI CASTG 5 cut(s) 505, 535, 582, 1020, 1038
Tth111I GACNNNGTC 1 cut(s) 429
VpaK11BI GGWCC 2 cut(s) 139, 1058
XagI CCTNNNNNAGG 1 cut(s) 932
XapI RAATTY 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 2 cut(s) 114, 198
XspI CTAG 1 cut(s) 801
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.