pycom11g25650

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002295
Physical Location & Seq
Forward (+)
28395736 .. 28399572
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g25650.11

Sequence Viewer

Length: 1338 bp
ATGTCCGGCCTCACCTTGGGATTGATGTCGCTCAACCTCGTCGACCTCGAAATCCTCCAGCGCAGCGGTAGCGCCACCGAGAAGAAGCAAGCCGCTAAGATACTGCCCGTTGTGCAAAAGCAGCATCAGCTTTTGGTCACTCTGCTTCTCTGCAATGCTTGTGCCATGGAGGCGCTTCCTTTATACCTTGATAAAATCTTTCACCCAGTTGTTGCTGTTGTGCTGTCTGTAACTTTTGTTCTGTTATTCGGGGAGGTTATTCCACAATCTATATGCTCAAGATATGGACTTTCGGTTGGTGCCAATTTTGTTTGGCTTGTTCGCATTCTTATGGTCATCTGTTATCCGATTGCATACCCAATTGCAAAGGTTCTTGATAAAGTACTTGGACATAGTGATGATTTGTTTAGACGAGCTCAGTTGAAGGCCCTCGTCTCTATTCATGGCCAAGAGGCTGGTAAGGGAGGTGAACTCACGCATGATGAGACAACGATCATCAGTGGAGCCCTGGATTTAACTGAAAAGACCGCAGAGGAAGCTATGACACCTATTGAATCAACATTTTCCTTGGATGTTAATTCAAACTTTGACTGGGAAGCAATTGGGAAAATTCTTGCACGAGGCCATAGTCGAGTCCCTGTCTATTATGAAAATCCGAAAAACATTATCGGCCTCCTTCTGGTGAAAAGTCTTCTCACAGTACGAGCTGAAGCAGAGATTCCAGTCAGTGCCGTTTCAATTCGAAGAATGCCTAGGGTTCCTGCAGATATGCCGCTGTATGATATACTTAATGAGTTTCAAAAAGGAAGCAGTCACCTGGCAGCTGTAGTGAAGAGCAAAGGAAAGAGCAACCTTCTGGCAATTCCTGACATAGAGAATTTCGAGGATAAACTGGCAAAGTTAAAATCTCAACCAGCTGCCTCCTTGCCAACCAAGCAAGATGAAAAATCAGGGAGTGTTTCTCTTGACATAGATAAAGCTCCAAGGTCTCTGGAAAACAAGCCAACCATTCAGCCAAATGGTACAATTCCAAATTCATCAGAGGACATTGAAGCCGGGGAGGTCATAGGAATCATCACCCTGGAGGATGTTTTTGAAGAACTTCTGCAAGAGGAAATTGTAGATGAGACAGATGTATATATCGATGTACATAGGAGGATACGTGTGGCTGCTGCAGCAGCTGCTTCATCCATGGCACGAGCTCCATCAACTCGGAAGTTGCTTGTAGGAGCTCTAAGCAAGCATGCGCAAACCCCAAAGAAGTCTGCCGAAGATGATGGGCATTCGACGAGGTTTTCAGGGTCTCCACGAGAGCCTCTTCTAGGCAGTAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
DUF21

Protein Analysis

446

Amino Acids

48.45

Weight (kDa)

6.11

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1248
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 42
AciI CCGC 4 cut(s) 66, 93, 528, 773
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 3 cut(s) 609, 877, 1033
AcuI CTGAAG 1 cut(s) 729
AfaI GTAC 4 cut(s) 384, 702, 1024, 1149
AfiI CCNNNNNNNGG 3 cut(s) 16, 679, 1322
AflIII ACRYGT 1 cut(s) 1162
AgsI TTSAA 7 cut(s) 424, 554, 582, 738, 800, 1052, 1097
AjnI CCWGG 3 cut(s) 507, 816, 1080
Alw21I GWGCWC 3 cut(s) 418, 1204, 1234
Alw26I GTCTC 5 cut(s) 439, 479, 993, 1121, 1308
AlwNI CAGNNNCTG 1 cut(s) 1181
AoxI GGCC 5 cut(s) 7, 426, 445, 622, 670
ApeKI GCWGC 9 cut(s) 63, 121, 821, 917, 1169, 1172, 1175, 1178, 1181
ApoI RAATTY 3 cut(s) 609, 877, 1033
Asp700I GAANNNNTTC 1 cut(s) 1101
AspA2I CCTAGG 1 cut(s) 752
AspLEI GCGC 4 cut(s) 63, 74, 175, 1249
AspS9I GGNCC 1 cut(s) 427
AsuC2I CCSGG 1 cut(s) 1057
AsuHPI GGTGA 6 cut(s) 4, 194, 479, 694, 806, 1069
AsuII TTCGAA 1 cut(s) 742
AvrII CCTAGG 1 cut(s) 752
BalI TGGCCA 1 cut(s) 447
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 4 cut(s) 418, 508, 1204, 1234
BauI CACGAG 3 cut(s) 618, 1197, 1308
BbsI GAAGAC 1 cut(s) 683
Bbv12I GWGCWC 3 cut(s) 418, 1204, 1234
BbvI GCAGC 9 cut(s) 75, 133, 833, 904, 1156, 1159, 1168, 1187, 1190
BccI CCATC 2 cut(s) 1213, 1271
BceAI ACGGC 1 cut(s) 716
BciT130I CCWGG 3 cut(s) 509, 818, 1082
BciVI GTATCC 1 cut(s) 1152
BcnI CCSGG 1 cut(s) 1057
BcoDI GTCTC 5 cut(s) 439, 479, 993, 1121, 1308
BfaI CTAG 2 cut(s) 753, 1322
BfmI CTRYAG 3 cut(s) 762, 825, 1173
BfoI RGCGCY 2 cut(s) 75, 176
BfuI GTATCC 1 cut(s) 1152
BglI GCCNNNNNGGC 1 cut(s) 170
BlnI CCTAGG 1 cut(s) 752
BmcAI AGTACT 1 cut(s) 384
Bme1390I CCNGG 4 cut(s) 509, 818, 1057, 1082
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 3 cut(s) 301, 505, 759
BmrFI CCNGG 4 cut(s) 509, 818, 1057, 1082
BmrI ACTGGG 2 cut(s) 200, 601
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 2 cut(s) 200, 601
BpiI GAAGAC 1 cut(s) 683
BplI GAGNNNNNCTC 4 cut(s) 456, 488, 946, 978
BpmI CTGGAG 2 cut(s) 41, 1103
Bpu14I TTCGAA 1 cut(s) 742
BpuEI CTTGAG 1 cut(s) 262
BpuMI CCSGG 1 cut(s) 1057
Bsa29I ATCGAT 1 cut(s) 1143
BsaAI YACGTR 1 cut(s) 1163
BsaI GGTCTC 2 cut(s) 993, 1308
BsaJI CCNNGG 9 cut(s) 15, 165, 507, 567, 752, 983, 1056, 1080, 1191
BsaXI ACNNNNNCTCC 2 cut(s) 1147, 1177
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 679, 1322
Bse1I ACTGG 4 cut(s) 206, 596, 722, 897
Bse3DI GCAATG 1 cut(s) 160
BseBI CCWGG 3 cut(s) 509, 818, 1082
BseCI ATCGAT 1 cut(s) 1143
BseDI CCNNGG 9 cut(s) 15, 165, 507, 567, 752, 983, 1056, 1080, 1191
BseGI GGATG 3 cut(s) 577, 1093, 1187
BseLI CCNNNNNNNGG 3 cut(s) 16, 679, 1322
BseMI GCAATG 1 cut(s) 160
BseMII CTCAG 1 cut(s) 431
BseNI ACTGG 4 cut(s) 206, 596, 722, 897
BseXI GCAGC 9 cut(s) 75, 133, 833, 904, 1156, 1159, 1168, 1187, 1190
BshFI GGCC 5 cut(s) 9, 428, 447, 624, 672
BshNI GGYRCC 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 1143
BsiHKAI GWGCWC 3 cut(s) 418, 1204, 1234
BsiSI CCGG 2 cut(s) 6, 1056
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 3 cut(s) 16, 679, 1322
BsmAI GTCTC 5 cut(s) 439, 479, 993, 1121, 1308
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 620
BsmI GAATGC 3 cut(s) 324, 753, 1282
BsnI GGCC 5 cut(s) 9, 428, 447, 624, 672
Bso31I GGTCTC 2 cut(s) 993, 1308
Bsp119I TTCGAA 1 cut(s) 742
Bsp1286I GDGCHC 4 cut(s) 418, 508, 1204, 1234
Bsp1407I TGTACA 1 cut(s) 1147
Bsp143I GATC 1 cut(s) 492
Bsp19I CCATGG 2 cut(s) 165, 1191
BspACI CCGC 4 cut(s) 66, 93, 528, 773
BspANI GGCC 5 cut(s) 9, 428, 447, 624, 672
BspCNI CTCAG 1 cut(s) 430
BspDI ATCGAT 1 cut(s) 1143
BspLI GGNNCC 3 cut(s) 301, 505, 759
BspMAI CTGCAG 2 cut(s) 766, 1177
BspQI GCTCTTC 1 cut(s) 827
BspT104I TTCGAA 1 cut(s) 742
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 2 cut(s) 993, 1308
BsrDI GCAATG 1 cut(s) 160
BsrGI TGTACA 1 cut(s) 1147
BsrI ACTGG 4 cut(s) 206, 596, 722, 897
BssECI CCNNGG 9 cut(s) 15, 165, 507, 567, 752, 983, 1056, 1080, 1191
BssMI GATC 1 cut(s) 492
BssSI CACGAG 3 cut(s) 618, 1197, 1308
BssT1I CCWWGG 6 cut(s) 15, 165, 567, 752, 983, 1191
Bst2BI CACGAG 3 cut(s) 618, 1197, 1308
Bst2UI CCWGG 3 cut(s) 509, 818, 1082
Bst4CI ACNGT 1 cut(s) 700
Bst6I CTCTTC 2 cut(s) 827, 1323
BstAPI GCANNNNNTGC 1 cut(s) 1181
BstAUI TGTACA 1 cut(s) 1147
BstBAI YACGTR 1 cut(s) 1163
BstBI TTCGAA 1 cut(s) 742
BstC8I GCNNGC 3 cut(s) 90, 1241, 1245
BstDEI CTNAG 3 cut(s) 96, 417, 1235
BstDSI CCRYGG 2 cut(s) 165, 1191
BstENI CCTNNNNNAGG 1 cut(s) 1320
BstF5I GGATG 3 cut(s) 577, 1093, 1187
BstH2I RGCGCY 2 cut(s) 75, 176
BstHHI GCGC 4 cut(s) 63, 74, 175, 1249
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 5 cut(s) 439, 479, 993, 1121, 1308
BstMBI GATC 1 cut(s) 492
BstNI CCWGG 3 cut(s) 509, 818, 1082
BstNSI RCATGY 1 cut(s) 1247
BstSCI CCNGG 4 cut(s) 507, 816, 1055, 1080
BstSFI CTRYAG 3 cut(s) 762, 825, 1173
BstV1I GCAGC 9 cut(s) 75, 133, 833, 904, 1156, 1159, 1168, 1187, 1190
BstV2I GAAGAC 1 cut(s) 683
BstXI CCANNNNNNTGG 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 1143
BsuI GTATCC 1 cut(s) 1152
BsuRI GGCC 5 cut(s) 9, 428, 447, 624, 672
BsuTUI ATCGAT 1 cut(s) 1143
BtgI CCRYGG 2 cut(s) 165, 1191
BtsCI GGATG 3 cut(s) 577, 1093, 1187
BtsIMutI CAGTG 2 cut(s) 505, 733
Cac8I GCNNGC 3 cut(s) 90, 1241, 1245
CaiI CAGNNNCTG 1 cut(s) 1181
CfoI GCGC 4 cut(s) 63, 74, 175, 1249
Cfr13I GGNCC 1 cut(s) 427
ClaI ATCGAT 1 cut(s) 1143
Csp6I GTAC 4 cut(s) 383, 701, 1023, 1148
CviAII CATG 5 cut(s) 166, 443, 479, 1192, 1244
CviQI GTAC 4 cut(s) 383, 701, 1023, 1148
DdeI CTNAG 3 cut(s) 96, 417, 1235
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 827, 1323
EarI CTCTTC 2 cut(s) 827, 1323
Ecl136II GAGCTC 3 cut(s) 416, 1202, 1232
Eco130I CCWWGG 6 cut(s) 15, 165, 567, 752, 983, 1191
Eco24I GRGCYC 4 cut(s) 418, 508, 1204, 1234
Eco31I GGTCTC 2 cut(s) 993, 1308
Eco53kI GAGCTC 3 cut(s) 416, 1202, 1232
Eco57I CTGAAG 1 cut(s) 729
EcoICRI GAGCTC 3 cut(s) 416, 1202, 1232
EcoNI CCTNNNNNAGG 1 cut(s) 1320
EcoO109I RGGNCCY 1 cut(s) 427
EcoRII CCWGG 3 cut(s) 507, 816, 1080
EcoT14I CCWWGG 6 cut(s) 15, 165, 567, 752, 983, 1191
EcoT38I GRGCYC 4 cut(s) 418, 508, 1204, 1234
ErhI CCWWGG 6 cut(s) 15, 165, 567, 752, 983, 1191
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 5 cut(s) 169, 446, 482, 1195, 1247
FaqI GGGAC 1 cut(s) 620
FatI CATG 5 cut(s) 165, 442, 478, 1191, 1243
FblI GTMKAC 1 cut(s) 42
FokI GGATG 3 cut(s) 584, 1100, 1174
FriOI GRGCYC 4 cut(s) 418, 508, 1204, 1234
FspBI CTAG 2 cut(s) 753, 1322
FspI TGCGCA 1 cut(s) 1248
GlaI GCGC 4 cut(s) 62, 73, 174, 1248
GsuI CTGGAG 2 cut(s) 41, 1103
HaeII RGCGCY 2 cut(s) 75, 176
HaeIII GGCC 5 cut(s) 9, 428, 447, 624, 672
HapII CCGG 2 cut(s) 6, 1056
HhaI GCGC 4 cut(s) 63, 74, 175, 1249
Hin1II CATG 5 cut(s) 169, 446, 482, 1195, 1247
Hin6I GCGC 4 cut(s) 61, 72, 173, 1247
HinP1I GCGC 4 cut(s) 61, 72, 173, 1247
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 4 cut(s) 554, 633, 718, 1071
HpaII CCGG 2 cut(s) 6, 1056
HphI GGTGA 6 cut(s) 4, 194, 479, 694, 806, 1069
Hpy166II GTNNAC 2 cut(s) 43, 470
Hpy188I TCNGA 4 cut(s) 348, 657, 1042, 1215
Hpy188III TCNNGA 5 cut(s) 279, 374, 866, 965, 992
Hpy8I GTNNAC 2 cut(s) 43, 470
Hpy99I CGWCG 2 cut(s) 44, 1291
HpyAV CCTTC 3 cut(s) 418, 686, 863
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4IV ACGT 1 cut(s) 1162
HpyCH4V TGCA 8 cut(s) 115, 153, 353, 365, 617, 764, 1108, 1175
HpyF3I CTNAG 3 cut(s) 96, 417, 1235
HpySE526I ACGT 1 cut(s) 1162
Hsp92II CATG 5 cut(s) 169, 446, 482, 1195, 1247
HspAI GCGC 4 cut(s) 61, 72, 173, 1247
Kzo9I GATC 1 cut(s) 492
LguI GCTCTTC 1 cut(s) 827
LmnI GCTCC 4 cut(s) 503, 985, 1207, 1229
Lsp1109I GCAGC 9 cut(s) 75, 133, 833, 904, 1156, 1159, 1168, 1187, 1190
LweI GCATC 1 cut(s) 133
MaeI CTAG 2 cut(s) 753, 1322
MaeII ACGT 1 cut(s) 1162
MaeIII GTNAC 3 cut(s) 136, 229, 812
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 7 cut(s) 94, 683, 756, 844, 1109, 1283, 1310
MfeI CAATTG 2 cut(s) 360, 600
MhlI GDGCHC 4 cut(s) 418, 508, 1204, 1234
MlsI TGGCCA 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 447
MlyI GAGTC 1 cut(s) 642
Mox20I TGGCCA 1 cut(s) 447
MroXI GAANNNNTTC 1 cut(s) 1101
MscI TGGCCA 1 cut(s) 447
MseI TTAA 4 cut(s) 515, 576, 789, 902
MslI CAYNNNNRTG 2 cut(s) 329, 396
Msp20I TGGCCA 1 cut(s) 447
MspA1I CMGCKG 5 cut(s) 66, 775, 824, 917, 1181
MspI CCGG 2 cut(s) 6, 1056
MspR9I CCNGG 4 cut(s) 509, 818, 1057, 1082
MunI CAATTG 2 cut(s) 360, 600
Mva1269I GAATGC 3 cut(s) 324, 753, 1282
MvaI CCWGG 3 cut(s) 509, 818, 1082
NciI CCSGG 1 cut(s) 1057
NcoI CCATGG 2 cut(s) 165, 1191
NdeII GATC 1 cut(s) 492
NlaIII CATG 5 cut(s) 169, 446, 482, 1195, 1247
NlaIV GGNNCC 3 cut(s) 301, 505, 759
NmuCI GTSAC 2 cut(s) 136, 812
NsbI TGCGCA 1 cut(s) 1248
NspI RCATGY 1 cut(s) 1247
NspV TTCGAA 1 cut(s) 742
PaeI GCATGC 1 cut(s) 1247
PciSI GCTCTTC 1 cut(s) 827
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 3 cut(s) 324, 753, 1282
PdmI GAANNNNTTC 1 cut(s) 1101
PfeI GAWTC 3 cut(s) 554, 718, 1071
PleI GAGTC 1 cut(s) 641
PpsI GAGTC 1 cut(s) 641
Ppu21I YACGTR 1 cut(s) 1163
Psp124BI GAGCTC 3 cut(s) 418, 1204, 1234
Psp6I CCWGG 3 cut(s) 507, 816, 1080
PspGI CCWGG 3 cut(s) 507, 816, 1080
PspN4I GGNNCC 3 cut(s) 301, 505, 759
PspPI GGNCC 1 cut(s) 427
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PstI CTGCAG 2 cut(s) 766, 1177
PstNI CAGNNNCTG 1 cut(s) 1181
PvuII CAGCTG 3 cut(s) 824, 917, 1181
RsaI GTAC 4 cut(s) 384, 702, 1024, 1149
RsaNI GTAC 4 cut(s) 383, 701, 1023, 1148
RseI CAYNNNNRTG 2 cut(s) 329, 396
SacI GAGCTC 3 cut(s) 418, 1204, 1234
SalI GTCGAC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 827
SaqAI TTAA 4 cut(s) 515, 576, 789, 902
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 1 cut(s) 427
ScaI AGTACT 1 cut(s) 384
SchI GAGTC 1 cut(s) 642
ScrFI CCNGG 4 cut(s) 509, 818, 1057, 1082
SduI GDGCHC 4 cut(s) 418, 508, 1204, 1234
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 3 cut(s) 762, 825, 1173
SfuI TTCGAA 1 cut(s) 742
SmiMI CAYNNNNRTG 2 cut(s) 329, 396
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
SphI GCATGC 1 cut(s) 1247
SsiI CCGC 4 cut(s) 66, 93, 528, 773
SspMI CTAG 2 cut(s) 753, 1322
SstI GAGCTC 3 cut(s) 418, 1204, 1234
StyD4I CCNGG 4 cut(s) 507, 816, 1055, 1080
StyI CCWWGG 6 cut(s) 15, 165, 567, 752, 983, 1191
TaaI ACNGT 1 cut(s) 700
TaiI ACGT 1 cut(s) 1165
TaqI TCGA 7 cut(s) 42, 48, 631, 742, 882, 1143, 1286
TatI WGTACW 2 cut(s) 382, 1147
TauI GCSGC 2 cut(s) 95, 775
TfiI GAWTC 3 cut(s) 554, 718, 1071
Tru1I TTAA 4 cut(s) 515, 576, 789, 902
Tru9I TTAA 4 cut(s) 515, 576, 789, 902
TscAI CASTG 2 cut(s) 505, 733
TseFI GTSAC 2 cut(s) 136, 812
TseI GCWGC 9 cut(s) 63, 121, 821, 917, 1169, 1172, 1175, 1178, 1181
Tsp45I GTSAC 2 cut(s) 136, 812
TspDTI ATGAA 5 cut(s) 431, 663, 957, 1026, 1176
TspRI CASTG 2 cut(s) 505, 733
XagI CCTNNNNNAGG 1 cut(s) 1320
XapI RAATTY 3 cut(s) 609, 877, 1033
XceI RCATGY 1 cut(s) 1247
XmaJI CCTAGG 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 42
XmnI GAANNNNTTC 1 cut(s) 1101
XspI CTAG 2 cut(s) 753, 1322
ZrmI AGTACT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.