Rh4AG214500

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002295
Physical Location & Seq
Forward (+)
50958052 .. 50961884
3833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG214500.1

Sequence Viewer

Length: 1227 bp
ATGCATCCCCTCAATGCAGTCATGATGACCCGGATGCTGGTTCGGAACAACAACAACAACAACTCCGGTCTGGAATCCGGGGGCATAGCCTTCGCGTCGATTTGGTGGTTTGTCTACATCGGCTTGTCGTGTTTCTTGGTCCTCTTCGCTGGAATAATGTCAGGCTTAACTTTAGGACTCATGTCCCTCGGCCTCGTCGACCTCGAGATTCTACAGCGAAGTGGTACTCCCACAGAGAAGAAGCAAGCAGCGACTATACTTCCGGTGGTTCAGAAGCAGCATCAGCTGCTTGTCACTCTGCTTCTGTGTAATGCTGGAGCTATGGAAGAAGGCAAAGGCGGTGAACTTACACATGATGAGACAACAATAATTAGTGGAGCACTGGACTTAACTGAAAAGACTGCTGAGGAGGCTATGACACCAATTGAATCAACCTTCTCCTTAGATGTCAATTCAAAGTTGGACTGGGAAGCAATGGGGAAAGTTCTTGCTCGGGGACATAGTCGAGTTCCTGTCTATTCTGGGAATTCAAGGAATATTATTGGACTTCTCCTGGTGAAAAGTCTTCTCACTGTACGACCCGAAACAGAGACCCCTGTCAGTGCTGTTTCCATCCGTAGAATCCCAAGAGTTCCCGCAGATATGCCACTGTATGATATATTGAATGAGTTTCAAAAGGGCAGCAGTCATATGGCTGCTGTGGTAAAGGCCAGAGGGAAAAGTAAGACTCATCCACCCACGATTGACGGAGAAAATAATGAAGGAAACAAAATTGCTGGCATGGACTCCCAACTGACTACTCCATTGCTATCCAAGCAGGATGGAAAGCCAGACCATGTTGTTGTTGACGTGCCCAAGATACCAAAGCCCACTAGTATGAAAATGGAAGCACTCTCACACTACAATGTTGGAGAAACAATTGGCCCCCCTTCGTCAGAGGATATTGAGGATGGTGAAGTCATTGGTATCATCACCCTGGAAGATGTATTTGAAGAACTCCTGCAGGAGGAGATTGTCGATGAAACGGATGAATATGTTGATGTACATAAAAGAATACGCGTTGCTGCAGCTGCAGCTGCTTCATCAGTGGCGCGAGCTCCATCACTGCGGAGGTTAAATGTCAACAAGGGACCAGGAGCCCAAACTAAGCAAGTAACCCCGAAGAAATCTACTGATGAATTGGCTGCAAGGCCACACGGGACTTTTGCTGATGGAAACAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

408

Amino Acids

44.14

Weight (kDa)

5.76

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 42 - 107 5.7e-09 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 114, 198
AccII CGCG 3 cut(s) 95, 1059, 1093
AciI CCGC 3 cut(s) 339, 636, 1108
AcsI RAATTY 1 cut(s) 526
AfaI GTAC 3 cut(s) 226, 576, 1044
AfiI CCNNNNNNNGG 2 cut(s) 37, 1006
AflIII ACRYGT 1 cut(s) 1057
AgsI TTSAA 6 cut(s) 428, 456, 531, 664, 674, 992
AhlI ACTAGT 1 cut(s) 872
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 3 cut(s) 552, 975, 1132
AluBI AGCT 5 cut(s) 286, 320, 1070, 1076, 1097
AluI AGCT 5 cut(s) 286, 320, 1070, 1076, 1097
Alw21I GWGCWC 2 cut(s) 382, 1099
Alw26I GTCTC 2 cut(s) 353, 584
Ama87I CYCGRG 2 cut(s) 203, 492
AoxI GGCC 4 cut(s) 190, 708, 922, 1190
ApoI RAATTY 1 cut(s) 526
AspLEI GCGC 1 cut(s) 1093
AspS9I GGNCC 3 cut(s) 139, 923, 1130
AsuC2I CCSGG 2 cut(s) 31, 79
AsuHPI GGTGA 4 cut(s) 353, 568, 964, 965
AvaI CYCGRG 2 cut(s) 203, 492
AvaII GGWCC 2 cut(s) 139, 1130
BaeGI GKGCMC 1 cut(s) 855
BanII GRGCYC 2 cut(s) 1099, 1141
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 2 cut(s) 382, 1099
BbvCI CCTCAGC 1 cut(s) 405
BccI CCATC 5 cut(s) 620, 815, 944, 1108, 1205
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 3 cut(s) 554, 977, 1134
BcnI CCSGG 2 cut(s) 31, 79
BcoDI GTCTC 2 cut(s) 353, 584
BcuI ACTAGT 1 cut(s) 872
BfaI CTAG 1 cut(s) 873
BfmI CTRYAG 4 cut(s) 212, 1001, 1065, 1071
Bme1390I CCNGG 5 cut(s) 31, 79, 554, 977, 1134
Bme18I GGWCC 2 cut(s) 139, 1130
BmeT110I CYCGRG 2 cut(s) 203, 492
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 3 cut(s) 139, 923, 1130
BmiI GGNNCC 3 cut(s) 925, 1131, 1138
BmrFI CCNGG 5 cut(s) 31, 79, 554, 977, 1134
BmrI ACTGGG 1 cut(s) 475
BmsI GCATC 3 cut(s) 13, 24, 289
BmuI ACTGGG 1 cut(s) 475
BoxI GACNNNNGTC 2 cut(s) 181, 596
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 2 cut(s) 31, 79
BsaI GGTCTC 1 cut(s) 584
BsaJI CCNNGG 3 cut(s) 78, 187, 975
BsaWI WCCGGW 2 cut(s) 65, 262
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 1006
Bse1I ACTGG 2 cut(s) 387, 470
Bse3DI GCAATG 2 cut(s) 480, 803
BseBI CCWGG 3 cut(s) 554, 977, 1134
BseDI CCNNGG 3 cut(s) 78, 187, 975
BseGI GGATG 7 cut(s) 4, 39, 612, 730, 826, 955, 1033
BseLI CCNNNNNNNGG 2 cut(s) 37, 1006
BseMI GCAATG 2 cut(s) 480, 803
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 387, 470
BseRI GAGGAG 2 cut(s) 422, 1022
BseSI GKGCMC 1 cut(s) 855
Bsh1236I CGCG 3 cut(s) 95, 1059, 1093
BshFI GGCC 4 cut(s) 192, 710, 924, 1192
BsiHKAI GWGCWC 2 cut(s) 382, 1099
BsiHKCI CYCGRG 2 cut(s) 203, 492
BsiSI CCGG 4 cut(s) 31, 66, 78, 263
BslFI GGGAC 4 cut(s) 169, 510, 1143, 1213
BslI CCNNNNNNNGG 2 cut(s) 37, 1006
BsmAI GTCTC 2 cut(s) 353, 584
BsmFI GGGAC 4 cut(s) 169, 510, 1143, 1213
BsnI GGCC 4 cut(s) 192, 710, 924, 1192
Bso31I GGTCTC 1 cut(s) 584
BsoBI CYCGRG 2 cut(s) 203, 492
Bsp1286I GDGCHC 4 cut(s) 382, 855, 1099, 1141
Bsp1407I TGTACA 1 cut(s) 1042
BspACI CCGC 3 cut(s) 339, 636, 1108
BspANI GGCC 4 cut(s) 192, 710, 924, 1192
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 3 cut(s) 95, 1059, 1093
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 925, 1131, 1138
BspMAI CTGCAG 3 cut(s) 1005, 1069, 1075
BspTNI GGTCTC 1 cut(s) 584
BsrDI GCAATG 2 cut(s) 480, 803
BsrGI TGTACA 1 cut(s) 1042
BsrI ACTGG 2 cut(s) 387, 470
BssECI CCNNGG 3 cut(s) 78, 187, 975
Bst2UI CCWGG 3 cut(s) 554, 977, 1134
Bst4CI ACNGT 2 cut(s) 574, 651
Bst6I CTCTTC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 286
BstAUI TGTACA 1 cut(s) 1042
BstC8I GCNNGC 3 cut(s) 246, 778, 1095
BstDEI CTNAG 3 cut(s) 405, 442, 1146
BstENI CCTNNNNNAGG 1 cut(s) 1004
BstF5I GGATG 7 cut(s) 4, 39, 612, 730, 826, 955, 1033
BstFNI CGCG 3 cut(s) 95, 1059, 1093
BstHHI GCGC 1 cut(s) 1093
BstMAI GTCTC 2 cut(s) 353, 584
BstMWI GCNNNNNNNGC 7 cut(s) 283, 286, 410, 814, 1070, 1073, 1076
BstNI CCWGG 3 cut(s) 554, 977, 1134
BstPAI GACNNNNGTC 2 cut(s) 181, 596
BstSCI CCNGG 5 cut(s) 29, 77, 552, 975, 1132
BstSFI CTRYAG 4 cut(s) 212, 1001, 1065, 1071
BstSLI GKGCMC 1 cut(s) 855
BstUI CGCG 3 cut(s) 95, 1059, 1093
BstV2I GAAGAC 1 cut(s) 557
BsuRI GGCC 4 cut(s) 192, 710, 924, 1192
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 7 cut(s) 4, 39, 612, 730, 826, 955, 1033
BtsI GCAGTG 1 cut(s) 1103
BtsIMutI CAGTG 6 cut(s) 380, 570, 607, 647, 1092, 1103
Cac8I GCNNGC 3 cut(s) 246, 778, 1095
CciI TCATGA 1 cut(s) 21
CfoI GCGC 1 cut(s) 1093
Cfr13I GGNCC 3 cut(s) 139, 923, 1130
CseI GACGC 1 cut(s) 84
Csp6I GTAC 3 cut(s) 225, 575, 1043
CviAII CATG 5 cut(s) 22, 181, 353, 781, 836
CviQI GTAC 3 cut(s) 225, 575, 1043
DdeI CTNAG 3 cut(s) 405, 442, 1146
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 1097
Eco24I GRGCYC 2 cut(s) 1099, 1141
Eco31I GGTCTC 1 cut(s) 584
Eco47I GGWCC 2 cut(s) 139, 1130
Eco53kI GAGCTC 1 cut(s) 1097
Eco88I CYCGRG 2 cut(s) 203, 492
EcoICRI GAGCTC 1 cut(s) 1097
EcoNI CCTNNNNNAGG 1 cut(s) 1004
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 3 cut(s) 552, 975, 1132
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1099, 1141
FaeI CATG 5 cut(s) 25, 184, 356, 784, 839
FaqI GGGAC 4 cut(s) 169, 510, 1143, 1213
FatI CATG 5 cut(s) 21, 180, 352, 780, 835
FauI CCCGC 1 cut(s) 643
FauNDI CATATG 1 cut(s) 690
FblI GTMKAC 2 cut(s) 114, 198
FokI GGATG 6 cut(s) 46, 599, 717, 833, 962, 1040
FriOI GRGCYC 2 cut(s) 1099, 1141
FspBI CTAG 1 cut(s) 873
GlaI GCGC 1 cut(s) 1092
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 4 cut(s) 192, 710, 924, 1192
HapII CCGG 4 cut(s) 31, 66, 78, 263
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 1093
Hin1II CATG 5 cut(s) 25, 184, 356, 784, 839
Hin6I GCGC 1 cut(s) 1091
HinP1I GCGC 1 cut(s) 1091
HincII GTYRAC 3 cut(s) 199, 847, 1123
HindII GTYRAC 3 cut(s) 199, 847, 1123
HinfI GANTC 7 cut(s) 74, 177, 208, 428, 621, 727, 785
HpaII CCGG 4 cut(s) 31, 66, 78, 263
HphI GGTGA 4 cut(s) 353, 568, 964, 965
Hpy166II GTNNAC 5 cut(s) 115, 199, 344, 847, 1123
Hpy188I TCNGA 3 cut(s) 45, 273, 937
Hpy188III TCNNGA 3 cut(s) 22, 71, 205
Hpy8I GTNNAC 5 cut(s) 115, 199, 344, 847, 1123
Hpy99I CGWCG 2 cut(s) 100, 200
HpyAV CCTTC 5 cut(s) 100, 323, 445, 755, 939
HpyCH4III ACNGT 2 cut(s) 574, 651
HpyCH4IV ACGT 1 cut(s) 849
HpyCH4V TGCA 6 cut(s) 4, 17, 1003, 1067, 1073, 1187
HpyF10VI GCNNNNNNNGC 7 cut(s) 283, 286, 410, 814, 1070, 1073, 1076
HpyF3I CTNAG 3 cut(s) 405, 442, 1146
HpySE526I ACGT 1 cut(s) 849
Hsp92II CATG 5 cut(s) 25, 184, 356, 784, 839
HspAI GCGC 1 cut(s) 1091
LmnI GCTCC 4 cut(s) 317, 377, 1102, 1136
LweI GCATC 3 cut(s) 13, 24, 289
MaeI CTAG 1 cut(s) 873
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 2 cut(s) 292, 1153
MboII GAAGA 7 cut(s) 136, 250, 338, 557, 992, 1004, 1174
MfeI CAATTG 2 cut(s) 423, 918
MhlI GDGCHC 4 cut(s) 382, 855, 1099, 1141
MluCI AATT 7 cut(s) 369, 423, 451, 526, 771, 918, 1178
MluI ACGCGT 1 cut(s) 1057
MlyI GAGTC 3 cut(s) 171, 721, 779
MmeI TCCRAC 2 cut(s) 441, 889
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 167, 389, 1115
MslI CAYNNNNRTG 2 cut(s) 875, 903
MspA1I CMGCKG 3 cut(s) 286, 1070, 1076
MspI CCGG 4 cut(s) 31, 66, 78, 263
MspR9I CCNGG 5 cut(s) 31, 79, 554, 977, 1134
MunI CAATTG 2 cut(s) 423, 918
MvaI CCWGG 3 cut(s) 554, 977, 1134
MvnI CGCG 3 cut(s) 95, 1059, 1093
MwoI GCNNNNNNNGC 7 cut(s) 283, 286, 410, 814, 1070, 1073, 1076
NciI CCSGG 2 cut(s) 31, 79
NdeI CATATG 1 cut(s) 690
NlaIII CATG 5 cut(s) 25, 184, 356, 784, 839
NlaIV GGNNCC 3 cut(s) 925, 1131, 1138
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 201
PfeI GAWTC 4 cut(s) 74, 208, 428, 621
PflFI GACNNNGTC 1 cut(s) 501
PleI GAGTC 3 cut(s) 171, 721, 779
PpsI GAGTC 3 cut(s) 171, 721, 779
PshAI GACNNNNGTC 2 cut(s) 181, 596
Psp124BI GAGCTC 1 cut(s) 1099
Psp6I CCWGG 3 cut(s) 552, 975, 1132
PspGI CCWGG 3 cut(s) 552, 975, 1132
PspN4I GGNNCC 3 cut(s) 925, 1131, 1138
PspPI GGNCC 3 cut(s) 139, 923, 1130
PstI CTGCAG 3 cut(s) 1005, 1069, 1075
PsyI GACNNNGTC 1 cut(s) 501
PvuII CAGCTG 3 cut(s) 286, 1070, 1076
RsaI GTAC 3 cut(s) 226, 576, 1044
RsaNI GTAC 3 cut(s) 225, 575, 1043
RseI CAYNNNNRTG 2 cut(s) 875, 903
SacI GAGCTC 1 cut(s) 1099
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 167, 389, 1115
Sau96I GGNCC 3 cut(s) 139, 923, 1130
SbfI CCTGCAGG 1 cut(s) 1005
SchI GAGTC 3 cut(s) 171, 721, 779
ScrFI CCNGG 5 cut(s) 31, 79, 554, 977, 1134
SdaI CCTGCAGG 1 cut(s) 1005
SduI GDGCHC 4 cut(s) 382, 855, 1099, 1141
SetI ASST 9 cut(s) 204, 288, 322, 437, 852, 1072, 1078, 1099, 1115
SfaNI GCATC 3 cut(s) 13, 24, 289
SfcI CTRYAG 4 cut(s) 212, 1001, 1065, 1071
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 2 cut(s) 139, 1130
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 2 cut(s) 875, 903
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SpeI ACTAGT 1 cut(s) 872
Sse8387I CCTGCAGG 1 cut(s) 1005
Sse9I AATT 7 cut(s) 369, 423, 451, 526, 771, 918, 1178
SsiI CCGC 3 cut(s) 339, 636, 1108
SspI AATATT 1 cut(s) 538
SspMI CTAG 1 cut(s) 873
SstI GAGCTC 1 cut(s) 1099
StyD4I CCNGG 5 cut(s) 29, 77, 552, 975, 1132
TaaI ACNGT 2 cut(s) 574, 651
TaiI ACGT 1 cut(s) 852
TaqI TCGA 5 cut(s) 98, 198, 204, 505, 1017
TasI AATT 7 cut(s) 369, 423, 451, 526, 771, 918, 1178
TatI WGTACW 1 cut(s) 1042
TfiI GAWTC 4 cut(s) 74, 208, 428, 621
Tru1I TTAA 3 cut(s) 167, 389, 1115
Tru9I TTAA 3 cut(s) 167, 389, 1115
TscAI CASTG 6 cut(s) 387, 577, 607, 654, 1092, 1110
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 6 cut(s) 774, 893, 1035, 1044, 1071, 1191
TspGWI ACGGA 3 cut(s) 605, 762, 1040
TspRI CASTG 6 cut(s) 387, 577, 607, 654, 1092, 1110
Tth111I GACNNNGTC 1 cut(s) 501
VpaK11BI GGWCC 2 cut(s) 139, 1130
XagI CCTNNNNNAGG 1 cut(s) 1004
XapI RAATTY 1 cut(s) 526
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 2 cut(s) 114, 198
XspI CTAG 1 cut(s) 873
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.