MD16G1183100.v1.1

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002295
Physical Location & Seq
Reverse (-)
15729283 .. 15733558
4276 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1183100.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGCATCCGATAAATGCGATCATGATGACTCGGATGCTGACTCGGAACTCGGGCCTCGCATCAGAGATTGGCGCCATCCCTTTCGGATCCGCCTGGTGGTTCATTTACGCCGGCCTCTCCTGTTTCTTTGTCATCTTCGCCGGTATAATGTCCGGCCTAACCCTCGGCCTCATGTCCCTCGGCCTCGTCGACCTCGAGATTCTGCAGCGCAGCGGCTCCCCGACCGAGAAGAAACAAGCAGCTATTATATTGCCGGTGGTTCAGAAGCAGCACCAGCTGCTCGTCACTTTGCTTCTGTGTAATGCGGCTGCTATGGAGGCTCTTCCCTTATATTTGGATAAACTGTTCAATCAATATGTTGCTATAATTCTCTCTGTGACTTTTGTCCTGTTTTTCGGGGAGGTTATTCCCCAAGCAATATGCACTAGATACGGACTTGCTGTTGGCTCGAACTTTGTGTGGCTTGTGAGAATTTTAATGTTTGTTTGCTATCCAATTGCTTTTCCAATTGGCAAGATCTTGGACTGTGTTCTTGGGCATAATGAAGCATTATTTAGGCGAGCTCAGTTAAAAGCCCTTGTCTCCATCCACAGCCAGGAGGAGGGCAAGGGTGGTGAGCTTACACACGATGAGACAACGATAATTAGTGGAGCCCTCGATTTAACTGAAAAGACTGCTGAGGAGGCTATGACGCCTATCGAATCTACCTTCTCCTTAGATGTCAATTCAAAATTGGACTGGGAAGCAATGGGGAAAGTTCTTGCTCGTGGACATAGTCGAGTTCCTGTCTATTCTGGGAACTCAAAGAATATTATTGGACTTCTGCTGGTGAAAAGTCTTCTCACTGTACGACCTGAAACAGAGACCCCAGTTAGTGCTGTTTCCATCCGTAGAATTCCAAGAGTTCCTGCAGATATGCCACTGTATGATATATTGAATGAGTTTCAAAAGGGCAGCAGTCATATGGCTGCTGTGGTGAAGCCTAAAGCGAAAAGTAAAATTCCTCGACCAACCATTGATGGAAAGAGAGATGAATGGAACGGCGACACTGGCATGGACTCCCAACTGACTACTCCTTTGCTATCCAAGCAGGATGGAAAGCCGGACAGTGTTGTTGTTGATATACCCAGGATACCAAAGCCCCCTCATAGACACAAGGAAACTTTTTCACCCCATAGTGAGAAAGCAACAAATGGTTTGCTCCAGGTATCAGAGGATATCGAGGAGGGTGAAGTCATTGGGATTATCACTCTGGAGGATGTATTTGAAGAACTCCTGCAGGAGGAGATTGTGGATGAAACAGATGAATATGTTGATGTACATAAAAGAATACGTGTCGCTGCAGCTGCAGCTGCTTCATCAGTGGCACGGGCTCCATCGACTCGGAGGTTAAATGTGAACAAGGGACCTGCCACTGGACAAAATGCCAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

479

Amino Acids

52.38

Weight (kDa)

6.29

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 40 - 209 6.4e-34 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1417
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 189
AciI CCGC 3 cut(s) 90, 213, 305
AclWI GGATC 2 cut(s) 81, 94
AcsI RAATTY 3 cut(s) 471, 894, 999
AcyI GRCGYC 2 cut(s) 72, 692
AfaI GTAC 2 cut(s) 849, 1320
AfiI CCNNNNNNNGG 5 cut(s) 96, 595, 601, 1282, 1415
AflIII ACRYGT 1 cut(s) 1333
AgsI TTSAA 5 cut(s) 349, 729, 937, 947, 1268
AjnI CCWGG 4 cut(s) 92, 594, 1127, 1203
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 6 cut(s) 242, 277, 563, 619, 1346, 1352
AluI AGCT 6 cut(s) 242, 277, 563, 619, 1346, 1352
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 3 cut(s) 586, 626, 857
AlwI GGATC 2 cut(s) 81, 94
Ama87I CYCGRG 2 cut(s) 49, 194
AoxI GGCC 5 cut(s) 52, 112, 154, 166, 181
ApoI RAATTY 3 cut(s) 471, 894, 999
ArsI GACNNNNNNTTYG 2 cut(s) 1060, 1092
AspLEI GCGC 2 cut(s) 74, 210
AspS9I GGNCC 2 cut(s) 52, 1406
AsuHPI GGTGA 5 cut(s) 626, 841, 988, 1161, 1241
AvaI CYCGRG 2 cut(s) 49, 194
AvaII GGWCC 1 cut(s) 1406
BamHI GGATCC 1 cut(s) 86
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 3 cut(s) 565, 655, 1375
BauI CACGAG 1 cut(s) 765
BbsI GAAGAC 1 cut(s) 830
Bbv12I GWGCWC 1 cut(s) 565
BbvCI CCTCAGC 1 cut(s) 678
BccI CCATC 6 cut(s) 83, 593, 893, 1013, 1088, 1384
BceAI ACGGC 1 cut(s) 1057
BciT130I CCWGG 4 cut(s) 94, 596, 1129, 1205
BciVI GTATCC 1 cut(s) 1125
BcoDI GTCTC 3 cut(s) 586, 626, 857
BfaI CTAG 1 cut(s) 426
BfmI CTRYAG 5 cut(s) 203, 909, 1277, 1341, 1347
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 1417
BfuI GTATCC 1 cut(s) 1125
BglII AGATCT 1 cut(s) 516
Bme1390I CCNGG 4 cut(s) 94, 596, 1129, 1205
Bme18I GGWCC 1 cut(s) 1406
BmeT110I CYCGRG 2 cut(s) 49, 194
BmgT120I GGNCC 2 cut(s) 52, 1406
BmiI GGNNCC 6 cut(s) 73, 88, 217, 652, 1374, 1407
BmrFI CCNGG 4 cut(s) 94, 596, 1129, 1205
BmrI ACTGGG 2 cut(s) 748, 863
BmsI GCATC 3 cut(s) 13, 24, 68
BmuI ACTGGG 2 cut(s) 748, 863
BoxI GACNNNNGTC 1 cut(s) 383
BpiI GAAGAC 1 cut(s) 830
BpmI CTGGAG 2 cut(s) 1187, 1274
Bpu10I CCTNAGC 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 1334
BsaHI GRCGYC 2 cut(s) 72, 692
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 3 cut(s) 163, 178, 1127
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 595, 601, 1282, 1415
Bse118I RCCGGY 3 cut(s) 110, 140, 253
Bse1I ACTGG 4 cut(s) 743, 869, 1054, 1420
Bse3DI GCAATG 1 cut(s) 753
BseBI CCWGG 4 cut(s) 94, 596, 1129, 1205
BseDI CCNNGG 3 cut(s) 163, 178, 1127
BseGI GGATG 8 cut(s) 4, 39, 75, 585, 885, 1099, 1264, 1300
BseLI CCNNNNNNNGG 5 cut(s) 96, 595, 601, 1282, 1415
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 2 cut(s) 578, 669
BseNI ACTGG 4 cut(s) 743, 869, 1054, 1420
BseRI GAGGAG 4 cut(s) 614, 695, 1238, 1298
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 5 cut(s) 54, 114, 156, 168, 183
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 565
BsiHKCI CYCGRG 2 cut(s) 49, 194
BsiSI CCGG 5 cut(s) 111, 141, 153, 254, 1103
BslFI GGGAC 2 cut(s) 160, 1419
BslI CCNNNNNNNGG 5 cut(s) 96, 595, 601, 1282, 1415
BsmAI GTCTC 3 cut(s) 586, 626, 857
BsmFI GGGAC 2 cut(s) 160, 1419
BsnI GGCC 5 cut(s) 54, 114, 156, 168, 183
Bso31I GGTCTC 1 cut(s) 857
BsoBI CYCGRG 2 cut(s) 49, 194
Bsp1286I GDGCHC 3 cut(s) 565, 655, 1375
Bsp1407I TGTACA 1 cut(s) 1318
Bsp143I GATC 3 cut(s) 18, 86, 516
BspACI CCGC 3 cut(s) 90, 213, 305
BspANI GGCC 5 cut(s) 54, 114, 156, 168, 183
BspCNI CTCAG 2 cut(s) 577, 670
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 6 cut(s) 73, 88, 217, 652, 1374, 1407
BspMAI CTGCAG 5 cut(s) 207, 913, 1281, 1345, 1351
BspMI ACCTGC 1 cut(s) 1417
BspPI GGATC 2 cut(s) 81, 94
BspQI GCTCTTC 1 cut(s) 327
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 857
BsrDI GCAATG 1 cut(s) 753
BsrFI RCCGGY 3 cut(s) 110, 140, 253
BsrGI TGTACA 1 cut(s) 1318
BsrI ACTGG 4 cut(s) 743, 869, 1054, 1420
BssAI RCCGGY 3 cut(s) 110, 140, 253
BssECI CCNNGG 3 cut(s) 163, 178, 1127
BssMI GATC 3 cut(s) 18, 86, 516
BssNI GRCGYC 2 cut(s) 72, 692
BssSI CACGAG 1 cut(s) 765
Bst2BI CACGAG 1 cut(s) 765
Bst2UI CCWGG 4 cut(s) 94, 596, 1129, 1205
Bst4CI ACNGT 5 cut(s) 345, 527, 847, 924, 1109
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 2 cut(s) 72, 692
BstAPI GCANNNNNTGC 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 1318
BstBAI YACGTR 1 cut(s) 1334
BstC8I GCNNGC 2 cut(s) 112, 561
BstDEI CTNAG 3 cut(s) 564, 678, 715
BstENI CCTNNNNNAGG 1 cut(s) 1280
BstF5I GGATG 8 cut(s) 4, 39, 75, 585, 885, 1099, 1264, 1300
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 210
BstKTI GATC 3 cut(s) 21, 89, 519
BstMAI GTCTC 3 cut(s) 586, 626, 857
BstMBI GATC 3 cut(s) 18, 86, 516
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 9 cut(s) 274, 277, 317, 683, 1050, 1087, 1346, 1349, 1352
BstNI CCWGG 4 cut(s) 94, 596, 1129, 1205
BstPAI GACNNNNGTC 1 cut(s) 383
BstSCI CCNGG 4 cut(s) 92, 594, 1127, 1203
BstSFI CTRYAG 5 cut(s) 203, 909, 1277, 1341, 1347
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 2 cut(s) 86, 516
BstYI RGATCY 2 cut(s) 86, 516
BsuI GTATCC 1 cut(s) 1125
BsuRI GGCC 5 cut(s) 54, 114, 156, 168, 183
BtsCI GGATG 8 cut(s) 4, 39, 75, 585, 885, 1099, 1264, 1300
BtsIMutI CAGTG 6 cut(s) 843, 920, 1047, 1114, 1368, 1413
BveI ACCTGC 1 cut(s) 1417
Cac8I GCNNGC 2 cut(s) 112, 561
CciI TCATGA 1 cut(s) 21
CfoI GCGC 2 cut(s) 74, 210
Cfr10I RCCGGY 3 cut(s) 110, 140, 253
Cfr13I GGNCC 2 cut(s) 52, 1406
CseI GACGC 1 cut(s) 700
Csp6I GTAC 2 cut(s) 848, 1319
CviAII CATG 3 cut(s) 22, 172, 1054
CviQI GTAC 2 cut(s) 848, 1319
DdeI CTNAG 3 cut(s) 564, 678, 715
DinI GGCGCC 1 cut(s) 73
DpnI GATC 3 cut(s) 20, 88, 518
DpnII GATC 3 cut(s) 18, 86, 516
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 563
Eco24I GRGCYC 3 cut(s) 565, 655, 1375
Eco31I GGTCTC 1 cut(s) 857
Eco32I GATATC 1 cut(s) 1219
Eco47I GGWCC 1 cut(s) 1406
Eco53kI GAGCTC 1 cut(s) 563
Eco88I CYCGRG 2 cut(s) 49, 194
EcoICRI GAGCTC 1 cut(s) 563
EcoNI CCTNNNNNAGG 1 cut(s) 1280
EcoO109I RGGNCCY 1 cut(s) 1406
EcoRI GAATTC 1 cut(s) 894
EcoRII CCWGG 4 cut(s) 92, 594, 1127, 1203
EcoRV GATATC 1 cut(s) 1219
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 565, 655, 1375
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
FaeI CATG 3 cut(s) 25, 175, 1057
FaqI GGGAC 2 cut(s) 160, 1419
FatI CATG 3 cut(s) 21, 171, 1053
FauNDI CATATG 1 cut(s) 963
FblI GTMKAC 1 cut(s) 189
FokI GGATG 7 cut(s) 46, 62, 572, 872, 1106, 1271, 1307
FriOI GRGCYC 3 cut(s) 565, 655, 1375
FspBI CTAG 1 cut(s) 426
GlaI GCGC 2 cut(s) 73, 209
GsuI CTGGAG 2 cut(s) 1187, 1274
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 5 cut(s) 54, 114, 156, 168, 183
HapII CCGG 5 cut(s) 111, 141, 153, 254, 1103
HgaI GACGC 1 cut(s) 700
HhaI GCGC 2 cut(s) 74, 210
Hin1I GRCGYC 2 cut(s) 72, 692
Hin1II CATG 3 cut(s) 25, 175, 1057
Hin6I GCGC 2 cut(s) 72, 208
HinP1I GCGC 2 cut(s) 72, 208
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 6 cut(s) 28, 40, 199, 701, 1058, 1381
HpaII CCGG 5 cut(s) 111, 141, 153, 254, 1103
HphI GGTGA 5 cut(s) 626, 841, 988, 1161, 1241
Hpy166II GTNNAC 3 cut(s) 190, 770, 1399
Hpy188I TCNGA 8 cut(s) 9, 33, 45, 64, 86, 264, 1213, 1386
Hpy188III TCNNGA 3 cut(s) 22, 196, 1253
Hpy8I GTNNAC 3 cut(s) 190, 770, 1399
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 718
HpyCH4III ACNGT 5 cut(s) 345, 527, 847, 924, 1109
HpyCH4IV ACGT 1 cut(s) 1333
HpyCH4V TGCA 7 cut(s) 4, 205, 423, 911, 1279, 1343, 1349
HpyF10VI GCNNNNNNNGC 9 cut(s) 274, 277, 317, 683, 1050, 1087, 1346, 1349, 1352
HpyF3I CTNAG 3 cut(s) 564, 678, 715
HpySE526I ACGT 1 cut(s) 1333
Hsp92I GRCGYC 2 cut(s) 72, 692
Hsp92II CATG 3 cut(s) 25, 175, 1057
HspAI GCGC 2 cut(s) 72, 208
KasI GGCGCC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 3 cut(s) 18, 86, 516
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 4 cut(s) 221, 650, 1206, 1378
LweI GCATC 3 cut(s) 13, 24, 68
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 1333
MaeIII GTNAC 2 cut(s) 283, 376
MalI GATC 3 cut(s) 20, 88, 518
MboI GATC 3 cut(s) 18, 86, 516
MboII GAAGA 5 cut(s) 127, 241, 314, 830, 1280
MfeI CAATTG 2 cut(s) 495, 507
MflI RGATCY 2 cut(s) 86, 516
MhlI GDGCHC 3 cut(s) 565, 655, 1375
MluCI AATT 9 cut(s) 366, 471, 495, 507, 642, 724, 731, 894, 999
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 4 cut(s) 22, 34, 1052, 1375
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 110
MseI TTAA 4 cut(s) 476, 569, 662, 1391
MslI CAYNNNNRTG 1 cut(s) 1052
MspA1I CMGCKG 4 cut(s) 213, 277, 1346, 1352
MspI CCGG 5 cut(s) 111, 141, 153, 254, 1103
MspR9I CCNGG 4 cut(s) 94, 596, 1129, 1205
MunI CAATTG 2 cut(s) 495, 507
MvaI CCWGG 4 cut(s) 94, 596, 1129, 1205
MwoI GCNNNNNNNGC 9 cut(s) 274, 277, 317, 683, 1050, 1087, 1346, 1349, 1352
NaeI GCCGGC 1 cut(s) 112
NarI GGCGCC 1 cut(s) 72
NdeI CATATG 1 cut(s) 963
NdeII GATC 3 cut(s) 18, 86, 516
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 3 cut(s) 25, 175, 1057
NlaIV GGNNCC 6 cut(s) 73, 88, 217, 652, 1374, 1407
NmeAIII GCCGAG 2 cut(s) 144, 159
NmuCI GTSAC 2 cut(s) 283, 376
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 194
PagI TCATGA 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 192
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 2 cut(s) 199, 701
PflFI GACNNNGTC 1 cut(s) 774
PleI GAGTC 4 cut(s) 22, 34, 1052, 1375
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 4 cut(s) 22, 34, 1052, 1375
Ppu21I YACGTR 1 cut(s) 1334
PpuMI RGGWCCY 1 cut(s) 1406
PshAI GACNNNNGTC 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 565
Psp5II RGGWCCY 1 cut(s) 1406
Psp6I CCWGG 4 cut(s) 92, 594, 1127, 1203
PspGI CCWGG 4 cut(s) 92, 594, 1127, 1203
PspN4I GGNNCC 6 cut(s) 73, 88, 217, 652, 1374, 1407
PspPI GGNCC 2 cut(s) 52, 1406
PspPPI RGGWCCY 1 cut(s) 1406
PstI CTGCAG 5 cut(s) 207, 913, 1281, 1345, 1351
PsuI RGATCY 2 cut(s) 86, 516
PsyI GACNNNGTC 1 cut(s) 774
PvuII CAGCTG 3 cut(s) 277, 1346, 1352
RsaI GTAC 2 cut(s) 849, 1320
RsaNI GTAC 2 cut(s) 848, 1319
RseI CAYNNNNRTG 1 cut(s) 1052
SacI GAGCTC 1 cut(s) 565
SalI GTCGAC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 4 cut(s) 476, 569, 662, 1391
Sau3AI GATC 3 cut(s) 18, 86, 516
Sau96I GGNCC 2 cut(s) 52, 1406
SbfI CCTGCAGG 1 cut(s) 1281
SchI GAGTC 4 cut(s) 22, 34, 1052, 1375
ScrFI CCNGG 4 cut(s) 94, 596, 1129, 1205
SdaI CCTGCAGG 1 cut(s) 1281
SduI GDGCHC 3 cut(s) 565, 655, 1375
SfaNI GCATC 3 cut(s) 13, 24, 68
SfcI CTRYAG 5 cut(s) 203, 909, 1277, 1341, 1347
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 1406
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 1052
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse8387I CCTGCAGG 1 cut(s) 1281
Sse9I AATT 9 cut(s) 366, 471, 495, 507, 642, 724, 731, 894, 999
SsiI CCGC 3 cut(s) 90, 213, 305
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 811
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 565
StyD4I CCNGG 4 cut(s) 92, 594, 1127, 1203
TaaI ACNGT 5 cut(s) 345, 527, 847, 924, 1109
TaiI ACGT 1 cut(s) 1336
TaqI TCGA 9 cut(s) 189, 195, 449, 657, 699, 778, 1006, 1221, 1379
TaqII GACCGA 1 cut(s) 239
TasI AATT 9 cut(s) 366, 471, 495, 507, 642, 724, 731, 894, 999
TatI WGTACW 1 cut(s) 1318
TauI GCSGC 2 cut(s) 216, 308
TfiI GAWTC 2 cut(s) 199, 701
Tru1I TTAA 4 cut(s) 476, 569, 662, 1391
Tru9I TTAA 4 cut(s) 476, 569, 662, 1391
TscAI CASTG 6 cut(s) 850, 927, 1054, 1114, 1368, 1420
TseFI GTSAC 2 cut(s) 283, 376
Tsp45I GTSAC 2 cut(s) 283, 376
TspDTI ATGAA 6 cut(s) 91, 558, 1047, 1311, 1320, 1347
TspGWI ACGGA 2 cut(s) 447, 878
TspRI CASTG 6 cut(s) 850, 927, 1054, 1114, 1368, 1420
Tth111I GACNNNGTC 1 cut(s) 774
VpaK11BI GGWCC 1 cut(s) 1406
XagI CCTNNNNNAGG 1 cut(s) 1280
XapI RAATTY 3 cut(s) 471, 894, 999
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.