Rroxscaffold_5G00361680

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002295
Physical Location & Seq
Forward (+)
42375967 .. 42380516
4550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00361680.1

Sequence Viewer

Length: 1539 bp
ATGCATCCCCTCAATGCAGTCATGATGACCCGGATGCTGGTTCGGAACAACAACAACTACTCCGGTCTGGAATCCGAGGGCATAGCCTTCGCATCCATTTGGTGGTTTGTCTATATCGGCTTGTCGTGTTTCTTGGTCCTCTTCGCCGGAATCATGTCAGGCTTAACTTTAGGACTCATGTCCCTCGGCCTCGTCGACCTCGAGATTCTACAGCGAAGTGGTACTCCCACCGAGAAAAAACAAGCAGCGACCATACTTCCGGTGGTTCAGAAGCAGCATCAGCTGCTCGTCACTTTGCTTCTGTGTAATGCTGGAGCTATGGAAGCGCTTCCTATATATTTGGACAAGCTTTTCAATCAGTATGTTGCTATAATTCTGTCTGTGACATTTGTGCTGTTTTTCGGAGAGGTTATTCCACAAGCAATATGCACTAGATATGGACTGGCTGTGGGTTCCAACTTTGTATGGCTTGTCCGAATTTTGATGATTATTTGCTACCCAATTGCTTATCCAATTGGAAAGATCTTGGATTGTGTTCTGGGACATAATGAAGCATTATTTAGGCGAGCACAGTTAAAAGCCCTTGTCTCCATCCACAGCAAGGAGGAGGGCAAAGGCGGTGAACTTACACATGATGAGACAACAATAATTAGTGGAGCACTGGACTTAACTGAAAAGACTGCGGAGGAGGCTATGACACCTATTGAATCAACCTTCTCCTTAGATGTCAATTCAAAGTTGGACTGGGAAGCAATGGGGAAAGTTCTTGCTCGGGGACATAGTCGAGTTCCTGTCTATTCTGGGAATTCAAGGAATATTATTGGACTTCTCCTGGTGAAAAGTCTTCTCACTGTACGACCTGAAACAGAGACCCCTGTCAGTGCTGTTTCCATCCGTAGAATCCCAAGAGTTCCCGCAGATATGCCACTGTATGATATATTGAATGAGTTTCAAAAGGGCAGCAGTCATATGGCTGCTGTGGTAAAGGCCAGAGGGAAAAGTAAGGCTCATCCACCCACGATTGACGGAGAAAATAATGAAGGAAACAAAATTGCTGGCATGGACTCCCAACTGACTACTCCATTGCTATCCAAGCAGGATGGAAAGCCAGACCATGTTGTTGTTGACGTACCCAAGATACCAAAGCCCACTAGTGTGAAAAAGGAAGCACTCTCACACTACAATGTTGGAGAAACAATTGGCCCCCCTTCGTCAGAGGATATTGAGGATGGTGAAGTCATTGGTATCATCACCCTGGAAGATGTATTTGAAGAACTCCTGCAGGAGGAGATTGTCGATGAAACGGATGAATATGTTGATGTACATAAAAGAATACGCGTTGCTGCAGCTGCAGCTGCTTCATCAGTGGCGCGAGCTCCATCACTGCGGAGGTTAAATGTCAACAAGGGACCAGTGAGTGAACACAACAAATTAGCCATATCCAGTGGAGCCCAAACTAAGCAAGTAACCCCGAAGAAATCTACTGATGAATTGGCTGCAAGGCCACACGGGACTTTTGCTGATGGAAACAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

512

Amino Acids

55.84

Weight (kDa)

6.26

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 41 - 210 7.3e-35 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 102
AccI GTMKAC 1 cut(s) 195
AccII CGCG 2 cut(s) 1338, 1372
AciI CCGC 4 cut(s) 618, 683, 915, 1387
AcsI RAATTY 2 cut(s) 477, 805
AfaI GTAC 4 cut(s) 223, 855, 1131, 1323
AfeI AGCGCT 1 cut(s) 327
AfiI CCNNNNNNNGG 4 cut(s) 37, 102, 601, 1285
AflIII ACRYGT 1 cut(s) 1336
AgsI TTSAA 7 cut(s) 355, 707, 735, 810, 943, 953, 1271
AhlI ACTAGT 1 cut(s) 1151
AjnI CCWGG 2 cut(s) 831, 1254
AleI CACNNNNGTG 1 cut(s) 1154
AluBI AGCT 6 cut(s) 283, 317, 349, 1349, 1355, 1376
AluI AGCT 6 cut(s) 283, 317, 349, 1349, 1355, 1376
Alw21I GWGCWC 3 cut(s) 571, 661, 1378
Alw26I GTCTC 3 cut(s) 592, 632, 863
Ama87I CYCGRG 2 cut(s) 200, 771
Aor51HI AGCGCT 1 cut(s) 327
AoxI GGCC 4 cut(s) 187, 987, 1201, 1502
ApoI RAATTY 2 cut(s) 477, 805
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 2 cut(s) 328, 1372
AspS9I GGNCC 3 cut(s) 136, 1202, 1409
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 4 cut(s) 632, 847, 1243, 1244
AvaI CYCGRG 2 cut(s) 200, 771
AvaII GGWCC 2 cut(s) 136, 1409
BanII GRGCYC 2 cut(s) 1378, 1453
BbsI GAAGAC 1 cut(s) 836
Bbv12I GWGCWC 3 cut(s) 571, 661, 1378
BccI CCATC 6 cut(s) 599, 899, 1094, 1223, 1387, 1517
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 2 cut(s) 833, 1256
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 3 cut(s) 592, 632, 863
BcuI ACTAGT 1 cut(s) 1151
BfaI CTAG 2 cut(s) 432, 1152
BfmI CTRYAG 4 cut(s) 209, 1280, 1344, 1350
BfoI RGCGCY 1 cut(s) 329
BglII AGATCT 1 cut(s) 522
Bme1390I CCNGG 3 cut(s) 31, 833, 1256
Bme18I GGWCC 2 cut(s) 136, 1409
BmeT110I CYCGRG 2 cut(s) 200, 771
BmgT120I GGNCC 3 cut(s) 136, 1202, 1409
BmiI GGNNCC 4 cut(s) 454, 1204, 1410, 1450
BmrFI CCNGG 3 cut(s) 31, 833, 1256
BmrI ACTGGG 1 cut(s) 754
BmsI GCATC 4 cut(s) 13, 24, 101, 286
BmuI ACTGGG 1 cut(s) 754
BoxI GACNNNNGTC 2 cut(s) 178, 875
BpiI GAAGAC 1 cut(s) 836
BpmI CTGGAG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 31
BsaI GGTCTC 1 cut(s) 863
BsaJI CCNNGG 3 cut(s) 75, 184, 1254
BsaWI WCCGGW 2 cut(s) 62, 259
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 102, 601, 1285
Bse1I ACTGG 5 cut(s) 447, 666, 749, 1412, 1443
Bse3DI GCAATG 2 cut(s) 759, 1082
BseBI CCWGG 2 cut(s) 833, 1256
BseDI CCNNGG 3 cut(s) 75, 184, 1254
BseGI GGATG 9 cut(s) 4, 39, 92, 591, 891, 1009, 1105, 1234, 1312
BseLI CCNNNNNNNGG 4 cut(s) 37, 102, 601, 1285
BseMI GCAATG 2 cut(s) 759, 1082
BseNI ACTGG 5 cut(s) 447, 666, 749, 1412, 1443
BseRI GAGGAG 3 cut(s) 620, 701, 1301
Bsh1236I CGCG 2 cut(s) 1338, 1372
BshFI GGCC 4 cut(s) 189, 989, 1203, 1504
BsiHKAI GWGCWC 3 cut(s) 571, 661, 1378
BsiHKCI CYCGRG 2 cut(s) 200, 771
BsiSI CCGG 4 cut(s) 31, 63, 147, 260
BslFI GGGAC 5 cut(s) 166, 555, 789, 1422, 1525
BslI CCNNNNNNNGG 4 cut(s) 37, 102, 601, 1285
BsmAI GTCTC 3 cut(s) 592, 632, 863
BsmFI GGGAC 5 cut(s) 166, 555, 789, 1422, 1525
BsnI GGCC 4 cut(s) 189, 989, 1203, 1504
Bso31I GGTCTC 1 cut(s) 863
BsoBI CYCGRG 2 cut(s) 200, 771
Bsp1286I GDGCHC 4 cut(s) 571, 661, 1378, 1453
Bsp1407I TGTACA 1 cut(s) 1321
Bsp143I GATC 1 cut(s) 522
BspACI CCGC 4 cut(s) 618, 683, 915, 1387
BspANI GGCC 4 cut(s) 189, 989, 1203, 1504
BspFNI CGCG 2 cut(s) 1338, 1372
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 4 cut(s) 454, 1204, 1410, 1450
BspMAI CTGCAG 3 cut(s) 1284, 1348, 1354
BspTNI GGTCTC 1 cut(s) 863
BsrDI GCAATG 2 cut(s) 759, 1082
BsrGI TGTACA 1 cut(s) 1321
BsrI ACTGG 5 cut(s) 447, 666, 749, 1412, 1443
BssECI CCNNGG 3 cut(s) 75, 184, 1254
BssMI GATC 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 833, 1256
Bst4CI ACNGT 3 cut(s) 573, 853, 930
Bst6I CTCTTC 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 283
BstAUI TGTACA 1 cut(s) 1321
BstC8I GCNNGC 3 cut(s) 567, 1057, 1374
BstDEI CTNAG 2 cut(s) 721, 1458
BstENI CCTNNNNNAGG 1 cut(s) 1283
BstF5I GGATG 9 cut(s) 4, 39, 92, 591, 891, 1009, 1105, 1234, 1312
BstFNI CGCG 2 cut(s) 1338, 1372
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 2 cut(s) 328, 1372
BstKTI GATC 1 cut(s) 525
BstMAI GTCTC 3 cut(s) 592, 632, 863
BstMBI GATC 1 cut(s) 522
BstMWI GCNNNNNNNGC 8 cut(s) 280, 283, 323, 689, 1093, 1349, 1352, 1355
BstNI CCWGG 2 cut(s) 833, 1256
BstPAI GACNNNNGTC 2 cut(s) 178, 875
BstSCI CCNGG 3 cut(s) 29, 831, 1254
BstSFI CTRYAG 4 cut(s) 209, 1280, 1344, 1350
BstUI CGCG 2 cut(s) 1338, 1372
BstV2I GAAGAC 1 cut(s) 836
BstX2I RGATCY 1 cut(s) 522
BstYI RGATCY 1 cut(s) 522
BsuRI GGCC 4 cut(s) 189, 989, 1203, 1504
BtsCI GGATG 9 cut(s) 4, 39, 92, 591, 891, 1009, 1105, 1234, 1312
BtsI GCAGTG 1 cut(s) 1382
BtsIMutI CAGTG 8 cut(s) 659, 849, 886, 926, 1371, 1382, 1419, 1450
Cac8I GCNNGC 3 cut(s) 567, 1057, 1374
CciI TCATGA 1 cut(s) 21
CfoI GCGC 2 cut(s) 328, 1372
Cfr13I GGNCC 3 cut(s) 136, 1202, 1409
Csp6I GTAC 4 cut(s) 222, 854, 1130, 1322
CviAII CATG 6 cut(s) 22, 154, 178, 632, 1060, 1115
CviQI GTAC 4 cut(s) 222, 854, 1130, 1322
DdeI CTNAG 2 cut(s) 721, 1458
DpnI GATC 1 cut(s) 524
DpnII GATC 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 1376
Eco24I GRGCYC 2 cut(s) 1378, 1453
Eco31I GGTCTC 1 cut(s) 863
Eco47I GGWCC 2 cut(s) 136, 1409
Eco47III AGCGCT 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 1376
Eco88I CYCGRG 2 cut(s) 200, 771
EcoICRI GAGCTC 1 cut(s) 1376
EcoNI CCTNNNNNAGG 1 cut(s) 1283
EcoRI GAATTC 1 cut(s) 805
EcoRII CCWGG 2 cut(s) 831, 1254
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1378, 1453
FaeI CATG 6 cut(s) 25, 157, 181, 635, 1063, 1118
FaqI GGGAC 5 cut(s) 166, 555, 789, 1422, 1525
FatI CATG 6 cut(s) 21, 153, 177, 631, 1059, 1114
FauI CCCGC 1 cut(s) 922
FauNDI CATATG 1 cut(s) 969
FblI GTMKAC 1 cut(s) 195
FokI GGATG 8 cut(s) 46, 79, 578, 878, 996, 1112, 1241, 1319
FriOI GRGCYC 2 cut(s) 1378, 1453
FspBI CTAG 2 cut(s) 432, 1152
GlaI GCGC 2 cut(s) 327, 1371
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 4 cut(s) 189, 989, 1203, 1504
HapII CCGG 4 cut(s) 31, 63, 147, 260
HhaI GCGC 2 cut(s) 328, 1372
Hin1II CATG 6 cut(s) 25, 157, 181, 635, 1063, 1118
Hin6I GCGC 2 cut(s) 326, 1370
HinP1I GCGC 2 cut(s) 326, 1370
HincII GTYRAC 3 cut(s) 196, 1126, 1402
HindII GTYRAC 3 cut(s) 196, 1126, 1402
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 7 cut(s) 71, 150, 174, 205, 707, 900, 1064
HpaII CCGG 4 cut(s) 31, 63, 147, 260
HphI GGTGA 4 cut(s) 632, 847, 1243, 1244
Hpy166II GTNNAC 5 cut(s) 196, 623, 1126, 1402, 1421
Hpy188I TCNGA 6 cut(s) 45, 76, 270, 404, 476, 1216
Hpy188III TCNNGA 3 cut(s) 22, 68, 202
Hpy8I GTNNAC 5 cut(s) 196, 623, 1126, 1402, 1421
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 4 cut(s) 97, 724, 1034, 1218
HpyCH4III ACNGT 3 cut(s) 573, 853, 930
HpyCH4IV ACGT 1 cut(s) 1128
HpyCH4V TGCA 7 cut(s) 4, 17, 429, 1282, 1346, 1352, 1499
HpyF10VI GCNNNNNNNGC 8 cut(s) 280, 283, 323, 689, 1093, 1349, 1352, 1355
HpyF3I CTNAG 2 cut(s) 721, 1458
HpySE526I ACGT 1 cut(s) 1128
Hsp92II CATG 6 cut(s) 25, 157, 181, 635, 1063, 1118
HspAI GCGC 2 cut(s) 326, 1370
Kzo9I GATC 1 cut(s) 522
LmnI GCTCC 4 cut(s) 314, 656, 1381, 1448
LweI GCATC 4 cut(s) 13, 24, 101, 286
MaeI CTAG 2 cut(s) 432, 1152
MaeII ACGT 1 cut(s) 1128
MaeIII GTNAC 3 cut(s) 289, 382, 1465
MalI GATC 1 cut(s) 524
MboI GATC 1 cut(s) 522
MboII GAAGA 5 cut(s) 133, 836, 1271, 1283, 1486
MfeI CAATTG 3 cut(s) 501, 513, 1197
MflI RGATCY 1 cut(s) 522
MhlI GDGCHC 4 cut(s) 571, 661, 1378, 1453
MluI ACGCGT 1 cut(s) 1336
MlyI GAGTC 2 cut(s) 168, 1058
MmeI TCCRAC 3 cut(s) 480, 720, 1168
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 4 cut(s) 164, 575, 668, 1394
MslI CAYNNNNRTG 2 cut(s) 1154, 1182
MspA1I CMGCKG 3 cut(s) 283, 1349, 1355
MspI CCGG 4 cut(s) 31, 63, 147, 260
MspR9I CCNGG 3 cut(s) 31, 833, 1256
MunI CAATTG 3 cut(s) 501, 513, 1197
MvaI CCWGG 2 cut(s) 833, 1256
MvnI CGCG 2 cut(s) 1338, 1372
MwoI GCNNNNNNNGC 8 cut(s) 280, 283, 323, 689, 1093, 1349, 1352, 1355
NciI CCSGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 969
NdeII GATC 1 cut(s) 522
NlaIII CATG 6 cut(s) 25, 157, 181, 635, 1063, 1118
NlaIV GGNNCC 4 cut(s) 454, 1204, 1410, 1450
NmeAIII GCCGAG 1 cut(s) 165
NmuCI GTSAC 2 cut(s) 289, 382
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 1154
PaeR7I CTCGAG 1 cut(s) 200
PagI TCATGA 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 198
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 5 cut(s) 71, 150, 205, 707, 900
PflFI GACNNNGTC 1 cut(s) 780
PflMI CCANNNNNTGG 1 cut(s) 102
PleI GAGTC 2 cut(s) 168, 1058
PpsI GAGTC 2 cut(s) 168, 1058
PshAI GACNNNNGTC 2 cut(s) 178, 875
Psp124BI GAGCTC 1 cut(s) 1378
Psp6I CCWGG 2 cut(s) 831, 1254
PspGI CCWGG 2 cut(s) 831, 1254
PspN4I GGNNCC 4 cut(s) 454, 1204, 1410, 1450
PspPI GGNCC 3 cut(s) 136, 1202, 1409
PstI CTGCAG 3 cut(s) 1284, 1348, 1354
PsuI RGATCY 1 cut(s) 522
PsyI GACNNNGTC 1 cut(s) 780
PvuII CAGCTG 3 cut(s) 283, 1349, 1355
RsaI GTAC 4 cut(s) 223, 855, 1131, 1323
RsaNI GTAC 4 cut(s) 222, 854, 1130, 1322
RseI CAYNNNNRTG 2 cut(s) 1154, 1182
SacI GAGCTC 1 cut(s) 1378
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 4 cut(s) 164, 575, 668, 1394
Sau3AI GATC 1 cut(s) 522
Sau96I GGNCC 3 cut(s) 136, 1202, 1409
SbfI CCTGCAGG 1 cut(s) 1284
SchI GAGTC 2 cut(s) 168, 1058
ScrFI CCNGG 3 cut(s) 31, 833, 1256
SdaI CCTGCAGG 1 cut(s) 1284
SduI GDGCHC 4 cut(s) 571, 661, 1378, 1453
SfaNI GCATC 4 cut(s) 13, 24, 101, 286
SfcI CTRYAG 4 cut(s) 209, 1280, 1344, 1350
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 2 cut(s) 136, 1409
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 2 cut(s) 1154, 1182
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
SpeI ACTAGT 1 cut(s) 1151
Sse8387I CCTGCAGG 1 cut(s) 1284
SsiI CCGC 4 cut(s) 618, 683, 915, 1387
SspI AATATT 1 cut(s) 817
SspMI CTAG 2 cut(s) 432, 1152
SstI GAGCTC 1 cut(s) 1378
StyD4I CCNGG 3 cut(s) 29, 831, 1254
TaaI ACNGT 3 cut(s) 573, 853, 930
TaiI ACGT 1 cut(s) 1131
TaqI TCGA 4 cut(s) 195, 201, 784, 1296
TatI WGTACW 1 cut(s) 1321
TfiI GAWTC 5 cut(s) 71, 150, 205, 707, 900
Tru1I TTAA 4 cut(s) 164, 575, 668, 1394
Tru9I TTAA 4 cut(s) 164, 575, 668, 1394
TscAI CASTG 8 cut(s) 666, 856, 886, 933, 1371, 1389, 1419, 1450
TseFI GTSAC 2 cut(s) 289, 382
Tsp45I GTSAC 2 cut(s) 289, 382
TspDTI ATGAA 6 cut(s) 564, 1053, 1314, 1323, 1350, 1503
TspGWI ACGGA 3 cut(s) 884, 1041, 1319
TspRI CASTG 8 cut(s) 666, 856, 886, 933, 1371, 1389, 1419, 1450
Tth111I GACNNNGTC 1 cut(s) 780
Van91I CCANNNNNTGG 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 136, 1409
XagI CCTNNNNNAGG 1 cut(s) 1283
XapI RAATTY 2 cut(s) 477, 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 2 cut(s) 432, 1152
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.