Rw4G018230

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002295
Physical Location & Seq
Forward (+)
40533244 .. 40537069
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018230.1

Sequence Viewer

Length: 1509 bp
ATGCATCCCCTCAATGCAGTCATGATGACCCGGATGCTGGTTCGGAACAACAACAACAACAACTCCGGTCTGGAATCCGGGGGCATAGCCTTCGCATCGATTTGGTGGTTTGTCTACATCGGCTTGTCGTGTTTCTTGGTCCTCTTCGCTGGAATAATGTCAGGCTTAACTTTAGGACTCATGTCCCTCGGCCTCGTCGACCTCGAGATTCTACAGCGAAGTGGTACTCCCACCGAGAAGAAGCAAGCAGCGACTATACTTCCGGTGGTTCAGAAGCAGCATCAGCTGCTCGTCACTTTGCTTCTGTGTAATGCTGGAGCTATGGAAGCGCTTCCTATATATTTGGACAAGCTTTTCAATCAGTATGTTGCTATTATTCTGTCTGTGACATTTGTGCTGTTTTTCGGAGAGGTTATTCCACAAGCAATATGTACTAGATATGGACTGGCTGTGGGTTCCAACTTTGTATGGCTTGTCCGAATTTTGATGATTATTTGCTACCCAATTGCTTATCCAATTGGAAAGATCTTGGATTGTGTTCTGGGACATAATGAGGCATTATTTAGGCGAGCTCAGTTAAAAGCCCTTGTCTCCATCCACAGCAAGGAGGAAGGCAAAGGCGGTGAACTTACACATGATGAGACAACAATAATTAGTGGAGCACTGGACTTAACTGAAAAGACTGCTGAGGAGGCTATGACACCTATTGAATCAACCTTCTCCTTAGATGTCAATTCAAAGTTGGACTGGGAAGCAATGGGGAAAGTTCTTGCTCGGGGACATAGTCGAGTTCCTGTCTATTCTGGGAATTCAAGGAATATTATTGGACTTCTCCTGGTGAAAAGTCTTCTCACTGTACGACCCGAAACAGAGACCCCTGTCAGTGCTGTTTCCATCCGTAGAATCCCAAGAGTTCCCGCAGATATGCCACTGTATGATATATTGAATGAGTTTCAAAAGGGCAGCAGTCATATGGCTGCTGTGGTAAAGGCCAGAGGGAAAAGTAAGACTCATCCACCCTCGATTGATGGAGAAAATAATGAAGGAAACAAAATTGCTGGCATGGACTCCCAACTGACTACTCCATTGCTATCCAAGCAGGATGGAAAGCCAGACCATGTTGTTGTTGACGTGCCCAAGATACCAAAGCCCACTAGTATGAAAAAGGAAGCACTCTCGCCCTACAATGTTGGAGAAACAATTGGCCCCCCTTCGTCAGAGGATATTGAGGATGGTGAAGTCATTGGTATCATCACCCTGGAAGATGTATTTGAAGAACTCCTGCAGGAGGAGATTGTCGATGAAACGGATGAATATGTTGATGTACATAAAAGAATACGCGTTGCTGCAGCTGCAGCTGCTTCATCAGTGGCGCGAGCTCCATCACTGCGGAGGTTAAATGTCAACAAGGGACCAGGAGCCCAAACTAAGCAAGTAACCCCGAAGAAATCTACTGATGAATTGGCTGCAAGGCCACACGGGACTTTTGCTGATGGAAACAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

502

Amino Acids

54.68

Weight (kDa)

6.29

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 42 - 211 7e-35 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 114, 198
AccII CGCG 2 cut(s) 1341, 1375
AciI CCGC 3 cut(s) 621, 918, 1390
AcsI RAATTY 2 cut(s) 480, 808
AfaI GTAC 4 cut(s) 226, 433, 858, 1326
AfeI AGCGCT 1 cut(s) 330
AfiI CCNNNNNNNGG 3 cut(s) 37, 604, 1288
AflIII ACRYGT 1 cut(s) 1339
AgsI TTSAA 7 cut(s) 358, 710, 738, 813, 946, 956, 1274
AhlI ACTAGT 1 cut(s) 1154
AjiI CACGTC 1 cut(s) 1132
AjnI CCWGG 3 cut(s) 834, 1257, 1414
AluBI AGCT 7 cut(s) 286, 320, 352, 572, 1352, 1358, 1379
AluI AGCT 7 cut(s) 286, 320, 352, 572, 1352, 1358, 1379
Alw21I GWGCWC 3 cut(s) 574, 664, 1381
Alw26I GTCTC 3 cut(s) 595, 635, 866
Ama87I CYCGRG 2 cut(s) 203, 774
Aor51HI AGCGCT 1 cut(s) 330
AoxI GGCC 4 cut(s) 190, 990, 1204, 1472
ApoI RAATTY 2 cut(s) 480, 808
Asp700I GAANNNNTTC 1 cut(s) 330
AspLEI GCGC 2 cut(s) 331, 1375
AspS9I GGNCC 3 cut(s) 139, 1205, 1412
AsuC2I CCSGG 2 cut(s) 31, 79
AsuHPI GGTGA 4 cut(s) 635, 850, 1246, 1247
AvaI CYCGRG 2 cut(s) 203, 774
AvaII GGWCC 2 cut(s) 139, 1412
BaeGI GKGCMC 1 cut(s) 1137
BanII GRGCYC 3 cut(s) 574, 1381, 1423
BbsI GAAGAC 1 cut(s) 839
Bbv12I GWGCWC 3 cut(s) 574, 664, 1381
BbvCI CCTCAGC 1 cut(s) 687
BccI CCATC 7 cut(s) 602, 902, 1022, 1097, 1226, 1390, 1487
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 3 cut(s) 836, 1259, 1416
BcnI CCSGG 2 cut(s) 31, 79
BcoDI GTCTC 3 cut(s) 595, 635, 866
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 2 cut(s) 435, 1155
BfmI CTRYAG 4 cut(s) 212, 1283, 1347, 1353
BfoI RGCGCY 1 cut(s) 332
BglII AGATCT 1 cut(s) 525
Bme1390I CCNGG 5 cut(s) 31, 79, 836, 1259, 1416
Bme18I GGWCC 2 cut(s) 139, 1412
BmeT110I CYCGRG 2 cut(s) 203, 774
BmgBI CACGTC 1 cut(s) 1132
BmgT120I GGNCC 3 cut(s) 139, 1205, 1412
BmiI GGNNCC 4 cut(s) 457, 1207, 1413, 1420
BmrFI CCNGG 5 cut(s) 31, 79, 836, 1259, 1416
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 4 cut(s) 13, 24, 104, 289
BmuI ACTGGG 1 cut(s) 757
BoxI GACNNNNGTC 2 cut(s) 181, 878
BpiI GAAGAC 1 cut(s) 839
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 687
BpuMI CCSGG 2 cut(s) 31, 79
Bsa29I ATCGAT 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 866
BsaJI CCNNGG 3 cut(s) 78, 187, 1257
BsaWI WCCGGW 2 cut(s) 65, 262
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 604, 1288
Bse1I ACTGG 3 cut(s) 450, 669, 752
Bse3DI GCAATG 2 cut(s) 762, 1085
BseBI CCWGG 3 cut(s) 836, 1259, 1416
BseCI ATCGAT 1 cut(s) 98
BseDI CCNNGG 3 cut(s) 78, 187, 1257
BseGI GGATG 8 cut(s) 4, 39, 594, 894, 1012, 1108, 1237, 1315
BseLI CCNNNNNNNGG 3 cut(s) 37, 604, 1288
BseMI GCAATG 2 cut(s) 762, 1085
BseMII CTCAG 2 cut(s) 587, 678
BseNI ACTGG 3 cut(s) 450, 669, 752
BseRI GAGGAG 2 cut(s) 704, 1304
BseSI GKGCMC 1 cut(s) 1137
Bsh1236I CGCG 2 cut(s) 1341, 1375
BshFI GGCC 4 cut(s) 192, 992, 1206, 1474
BshVI ATCGAT 1 cut(s) 98
BsiHKAI GWGCWC 3 cut(s) 574, 664, 1381
BsiHKCI CYCGRG 2 cut(s) 203, 774
BsiSI CCGG 4 cut(s) 31, 66, 78, 263
BslFI GGGAC 5 cut(s) 169, 558, 792, 1425, 1495
BslI CCNNNNNNNGG 3 cut(s) 37, 604, 1288
BsmAI GTCTC 3 cut(s) 595, 635, 866
BsmFI GGGAC 5 cut(s) 169, 558, 792, 1425, 1495
BsnI GGCC 4 cut(s) 192, 992, 1206, 1474
Bso31I GGTCTC 1 cut(s) 866
BsoBI CYCGRG 2 cut(s) 203, 774
Bsp1286I GDGCHC 5 cut(s) 574, 664, 1137, 1381, 1423
Bsp1407I TGTACA 1 cut(s) 1324
Bsp143I GATC 1 cut(s) 525
BspACI CCGC 3 cut(s) 621, 918, 1390
BspANI GGCC 4 cut(s) 192, 992, 1206, 1474
BspCNI CTCAG 2 cut(s) 586, 679
BspDI ATCGAT 1 cut(s) 98
BspFNI CGCG 2 cut(s) 1341, 1375
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 4 cut(s) 457, 1207, 1413, 1420
BspMAI CTGCAG 3 cut(s) 1287, 1351, 1357
BspTNI GGTCTC 1 cut(s) 866
BsrDI GCAATG 2 cut(s) 762, 1085
BsrGI TGTACA 1 cut(s) 1324
BsrI ACTGG 3 cut(s) 450, 669, 752
BssECI CCNNGG 3 cut(s) 78, 187, 1257
BssMI GATC 1 cut(s) 525
Bst2UI CCWGG 3 cut(s) 836, 1259, 1416
Bst4CI ACNGT 2 cut(s) 856, 933
Bst6I CTCTTC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 286
BstAUI TGTACA 1 cut(s) 1324
BstC8I GCNNGC 4 cut(s) 246, 570, 1060, 1377
BstDEI CTNAG 4 cut(s) 573, 687, 724, 1428
BstENI CCTNNNNNAGG 1 cut(s) 1286
BstF5I GGATG 8 cut(s) 4, 39, 594, 894, 1012, 1108, 1237, 1315
BstFNI CGCG 2 cut(s) 1341, 1375
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 2 cut(s) 331, 1375
BstKTI GATC 1 cut(s) 528
BstMAI GTCTC 3 cut(s) 595, 635, 866
BstMBI GATC 1 cut(s) 525
BstMWI GCNNNNNNNGC 8 cut(s) 283, 286, 326, 692, 1096, 1352, 1355, 1358
BstNI CCWGG 3 cut(s) 836, 1259, 1416
BstPAI GACNNNNGTC 2 cut(s) 181, 878
BstSCI CCNGG 5 cut(s) 29, 77, 834, 1257, 1414
BstSFI CTRYAG 4 cut(s) 212, 1283, 1347, 1353
BstSLI GKGCMC 1 cut(s) 1137
BstUI CGCG 2 cut(s) 1341, 1375
BstV2I GAAGAC 1 cut(s) 839
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
Bsu15I ATCGAT 1 cut(s) 98
BsuRI GGCC 4 cut(s) 192, 992, 1206, 1474
BsuTUI ATCGAT 1 cut(s) 98
BtrI CACGTC 1 cut(s) 1132
BtsCI GGATG 8 cut(s) 4, 39, 594, 894, 1012, 1108, 1237, 1315
BtsI GCAGTG 1 cut(s) 1385
BtsIMutI CAGTG 6 cut(s) 662, 852, 889, 929, 1374, 1385
Cac8I GCNNGC 4 cut(s) 246, 570, 1060, 1377
CciI TCATGA 1 cut(s) 21
CfoI GCGC 2 cut(s) 331, 1375
Cfr13I GGNCC 3 cut(s) 139, 1205, 1412
ClaI ATCGAT 1 cut(s) 98
Csp6I GTAC 4 cut(s) 225, 432, 857, 1325
CviAII CATG 5 cut(s) 22, 181, 635, 1063, 1118
CviQI GTAC 4 cut(s) 225, 432, 857, 1325
DdeI CTNAG 4 cut(s) 573, 687, 724, 1428
DpnI GATC 1 cut(s) 527
DpnII GATC 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 2 cut(s) 572, 1379
Eco24I GRGCYC 3 cut(s) 574, 1381, 1423
Eco31I GGTCTC 1 cut(s) 866
Eco47I GGWCC 2 cut(s) 139, 1412
Eco47III AGCGCT 1 cut(s) 330
Eco53kI GAGCTC 2 cut(s) 572, 1379
Eco88I CYCGRG 2 cut(s) 203, 774
EcoICRI GAGCTC 2 cut(s) 572, 1379
EcoNI CCTNNNNNAGG 1 cut(s) 1286
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 3 cut(s) 834, 1257, 1414
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 574, 1381, 1423
FaeI CATG 5 cut(s) 25, 184, 638, 1066, 1121
FaqI GGGAC 5 cut(s) 169, 558, 792, 1425, 1495
FatI CATG 5 cut(s) 21, 180, 634, 1062, 1117
FauI CCCGC 1 cut(s) 925
FauNDI CATATG 1 cut(s) 972
FblI GTMKAC 2 cut(s) 114, 198
FokI GGATG 7 cut(s) 46, 581, 881, 999, 1115, 1244, 1322
FriOI GRGCYC 3 cut(s) 574, 1381, 1423
FspBI CTAG 2 cut(s) 435, 1155
GlaI GCGC 2 cut(s) 330, 1374
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 4 cut(s) 192, 992, 1206, 1474
HapII CCGG 4 cut(s) 31, 66, 78, 263
HhaI GCGC 2 cut(s) 331, 1375
Hin1II CATG 5 cut(s) 25, 184, 638, 1066, 1121
Hin6I GCGC 2 cut(s) 329, 1373
HinP1I GCGC 2 cut(s) 329, 1373
HincII GTYRAC 3 cut(s) 199, 1129, 1405
HindII GTYRAC 3 cut(s) 199, 1129, 1405
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 7 cut(s) 74, 177, 208, 710, 903, 1009, 1067
HpaII CCGG 4 cut(s) 31, 66, 78, 263
HphI GGTGA 4 cut(s) 635, 850, 1246, 1247
Hpy166II GTNNAC 5 cut(s) 115, 199, 626, 1129, 1405
Hpy188I TCNGA 5 cut(s) 45, 273, 407, 479, 1219
Hpy188III TCNNGA 3 cut(s) 22, 71, 205
Hpy8I GTNNAC 5 cut(s) 115, 199, 626, 1129, 1405
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 5 cut(s) 100, 605, 727, 1037, 1221
HpyCH4III ACNGT 2 cut(s) 856, 933
HpyCH4IV ACGT 1 cut(s) 1131
HpyCH4V TGCA 6 cut(s) 4, 17, 1285, 1349, 1355, 1469
HpyF10VI GCNNNNNNNGC 8 cut(s) 283, 286, 326, 692, 1096, 1352, 1355, 1358
HpyF3I CTNAG 4 cut(s) 573, 687, 724, 1428
HpySE526I ACGT 1 cut(s) 1131
Hsp92II CATG 5 cut(s) 25, 184, 638, 1066, 1121
HspAI GCGC 2 cut(s) 329, 1373
Kzo9I GATC 1 cut(s) 525
LmnI GCTCC 4 cut(s) 317, 659, 1384, 1418
LweI GCATC 4 cut(s) 13, 24, 104, 289
MaeI CTAG 2 cut(s) 435, 1155
MaeII ACGT 1 cut(s) 1131
MaeIII GTNAC 3 cut(s) 292, 385, 1435
MalI GATC 1 cut(s) 527
MboI GATC 1 cut(s) 525
MboII GAAGA 6 cut(s) 136, 250, 839, 1274, 1286, 1456
MfeI CAATTG 3 cut(s) 504, 516, 1200
MflI RGATCY 1 cut(s) 525
MhlI GDGCHC 5 cut(s) 574, 664, 1137, 1381, 1423
MluCI AATT 9 cut(s) 480, 504, 516, 651, 733, 808, 1053, 1200, 1460
MluI ACGCGT 1 cut(s) 1339
MlyI GAGTC 3 cut(s) 171, 1003, 1061
MmeI TCCRAC 3 cut(s) 483, 723, 1171
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 4 cut(s) 167, 578, 671, 1397
MslI CAYNNNNRTG 1 cut(s) 1157
MspA1I CMGCKG 3 cut(s) 286, 1352, 1358
MspI CCGG 4 cut(s) 31, 66, 78, 263
MspR9I CCNGG 5 cut(s) 31, 79, 836, 1259, 1416
MunI CAATTG 3 cut(s) 504, 516, 1200
MvaI CCWGG 3 cut(s) 836, 1259, 1416
MvnI CGCG 2 cut(s) 1341, 1375
MwoI GCNNNNNNNGC 8 cut(s) 283, 286, 326, 692, 1096, 1352, 1355, 1358
NciI CCSGG 2 cut(s) 31, 79
NdeI CATATG 1 cut(s) 972
NdeII GATC 1 cut(s) 525
NlaIII CATG 5 cut(s) 25, 184, 638, 1066, 1121
NlaIV GGNNCC 4 cut(s) 457, 1207, 1413, 1420
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 2 cut(s) 292, 385
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 201
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 4 cut(s) 74, 208, 710, 903
PflFI GACNNNGTC 1 cut(s) 783
PleI GAGTC 3 cut(s) 171, 1003, 1061
PpsI GAGTC 3 cut(s) 171, 1003, 1061
PshAI GACNNNNGTC 2 cut(s) 181, 878
Psp124BI GAGCTC 2 cut(s) 574, 1381
Psp6I CCWGG 3 cut(s) 834, 1257, 1414
PspGI CCWGG 3 cut(s) 834, 1257, 1414
PspN4I GGNNCC 4 cut(s) 457, 1207, 1413, 1420
PspPI GGNCC 3 cut(s) 139, 1205, 1412
PstI CTGCAG 3 cut(s) 1287, 1351, 1357
PsuI RGATCY 1 cut(s) 525
PsyI GACNNNGTC 1 cut(s) 783
PvuII CAGCTG 3 cut(s) 286, 1352, 1358
RsaI GTAC 4 cut(s) 226, 433, 858, 1326
RsaNI GTAC 4 cut(s) 225, 432, 857, 1325
RseI CAYNNNNRTG 1 cut(s) 1157
SacI GAGCTC 2 cut(s) 574, 1381
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 4 cut(s) 167, 578, 671, 1397
Sau3AI GATC 1 cut(s) 525
Sau96I GGNCC 3 cut(s) 139, 1205, 1412
SbfI CCTGCAGG 1 cut(s) 1287
SchI GAGTC 3 cut(s) 171, 1003, 1061
ScrFI CCNGG 5 cut(s) 31, 79, 836, 1259, 1416
SdaI CCTGCAGG 1 cut(s) 1287
SduI GDGCHC 5 cut(s) 574, 664, 1137, 1381, 1423
SfaNI GCATC 4 cut(s) 13, 24, 104, 289
SfcI CTRYAG 4 cut(s) 212, 1283, 1347, 1353
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 2 cut(s) 139, 1412
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 1157
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SpeI ACTAGT 1 cut(s) 1154
Sse8387I CCTGCAGG 1 cut(s) 1287
Sse9I AATT 9 cut(s) 480, 504, 516, 651, 733, 808, 1053, 1200, 1460
SsiI CCGC 3 cut(s) 621, 918, 1390
SspI AATATT 1 cut(s) 820
SspMI CTAG 2 cut(s) 435, 1155
SstI GAGCTC 2 cut(s) 574, 1381
StyD4I CCNGG 5 cut(s) 29, 77, 834, 1257, 1414
TaaI ACNGT 2 cut(s) 856, 933
TaiI ACGT 1 cut(s) 1134
TaqI TCGA 6 cut(s) 98, 198, 204, 787, 1022, 1299
TasI AATT 9 cut(s) 480, 504, 516, 651, 733, 808, 1053, 1200, 1460
TatI WGTACW 2 cut(s) 431, 1324
TfiI GAWTC 4 cut(s) 74, 208, 710, 903
Tru1I TTAA 4 cut(s) 167, 578, 671, 1397
Tru9I TTAA 4 cut(s) 167, 578, 671, 1397
TscAI CASTG 6 cut(s) 669, 859, 889, 936, 1374, 1392
TseFI GTSAC 2 cut(s) 292, 385
Tsp45I GTSAC 2 cut(s) 292, 385
TspDTI ATGAA 6 cut(s) 1056, 1175, 1317, 1326, 1353, 1473
TspGWI ACGGA 2 cut(s) 887, 1322
TspRI CASTG 6 cut(s) 669, 859, 889, 936, 1374, 1392
Tth111I GACNNNGTC 1 cut(s) 783
VpaK11BI GGWCC 2 cut(s) 139, 1412
XagI CCTNNNNNAGG 1 cut(s) 1286
XapI RAATTY 2 cut(s) 480, 808
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 2 cut(s) 114, 198
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 435, 1155
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.