RLG00000007864

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002295
Physical Location & Seq
Reverse (-)
22180853 .. 22184625
3773 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007864

Sequence Viewer

Length: 1368 bp
ATGATGACCCGGATGCTGGTTCGGAACAACAACAACTCCGGTCTGGAATCCGAGGGCATAGCCTTCGCATCGATTTGGTGGTTTGTCTATATCGGCTTGTCGTGTTTCTTGGTCCTCTTCGCCGGAATAATGTCAGGCTTAACTTTAGGACTCATGTCCCTCGGCCTCGTCGACCTCGAGATTCTACAGCGAAGTGGTACTCCCACCGAGAAAAAACAAGCAGCGACTATACTTCCGGTGGTTCAGAAGCAGCATCAGCTGCTTGTCACTTTGCTTCTGTGTAATGCTGGAGCTATGGAAGCGCTTCCTATATATTTGGACAAGCTTTTCAATCAGTATGTTGCTATAATTCTGTCTGTGACATTTGTGCTGTTTTTCGGAGAGATCTTGGATTGTGTTCTGGGACATAATGAAGCATTATTTAGGCGAGCTCAGTTAAAAGCCCTTGTCTCCATCCACAGCAAGGAGGAGGGCAAAGGCGGTGAACTTACACATGATGAGACAACAATAATTAGTGGAGCACTGGACTTAACTGAAAAGACTGCTGAGGAGGCTATGACACCTATTGAATCAACCTTCTCCTTAGATGTCAATTCAAAGTTGGACTGGGAAGCAATGGGGAAAGTTCTTGCTCGGGGACATAGTCGAGTTCCTGTCTATTCTGGGAATTCAAGGAATATTATTGGACTTCTCCTGGTGAAAAGTCTTCTCACTGTACGACCCGAAACAGAGACCCCTGTCAGTGCTGTTTCCATCCGTAGAATCCCAAGAGTTCCCGCAGATATGCCACTGTATGATATATTGAATGAGTTTCAAAAGGGCAGCAGTCATATGGCTGCTGTGGTAAAGGCCAGAGGGAAAAGTAAGGCTCATCCACCCACGATTGACGGAGAAAATAATGAAGGAAACAAAATTGCTGGCATGGACTCCCAACTGACTACTCCATTGCTATCCAAGCAGGATGGAAAGCCAGACCATGTTGTTGTTGACGTACCCAAGATACCAAAGCCCACCAGTATGAAAAAGGAAGCACTCTCACACTACAATGTTGGAGAAACAATTGGCCCCCCTTCGTCAGAGGATATTGAGGATGGTGAAGTCATTGGTATCATCACCCTGGAAGATGTATTTGAAGAACTCCTGCAGGAGGAGATTGTCGATGAAACGGATGAATATGTTGACGTACATAAAAGAATACGCGTTGCTGCAGCTGCAGCTGCTTCATCAGTGGCGCGAGCTCCATCACTGCGGAGGTTAAATGTCAACAAGGGACCAGGAGCCCAAACTAAGCAAGTAACCCCGAAGAAATCTACTGATGAATTGGCTGCAAGGCCACACGGGACTTTTGCTGATGGAAACAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

456

Amino Acids

49.55

Weight (kDa)

5.84

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 33 - 130 5.7e-16 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 171
AccII CGCG 2 cut(s) 1200, 1234
AciI CCGC 3 cut(s) 480, 777, 1249
AcsI RAATTY 1 cut(s) 667
AfaI GTAC 4 cut(s) 199, 717, 993, 1185
AfeI AGCGCT 1 cut(s) 303
AfiI CCNNNNNNNGG 3 cut(s) 16, 463, 1147
AflIII ACRYGT 1 cut(s) 1198
AgsI TTSAA 7 cut(s) 331, 569, 597, 672, 805, 815, 1133
AjnI CCWGG 3 cut(s) 693, 1116, 1273
AluBI AGCT 7 cut(s) 259, 293, 325, 431, 1211, 1217, 1238
AluI AGCT 7 cut(s) 259, 293, 325, 431, 1211, 1217, 1238
Alw21I GWGCWC 3 cut(s) 433, 523, 1240
Alw26I GTCTC 3 cut(s) 454, 494, 725
Ama87I CYCGRG 2 cut(s) 176, 633
Aor51HI AGCGCT 1 cut(s) 303
AoxI GGCC 4 cut(s) 163, 849, 1063, 1331
ApoI RAATTY 1 cut(s) 667
Asp700I GAANNNNTTC 1 cut(s) 303
AspLEI GCGC 2 cut(s) 304, 1234
AspS9I GGNCC 3 cut(s) 112, 1064, 1271
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 4 cut(s) 494, 709, 1105, 1106
AvaI CYCGRG 2 cut(s) 176, 633
AvaII GGWCC 2 cut(s) 112, 1271
BanII GRGCYC 3 cut(s) 433, 1240, 1282
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 3 cut(s) 433, 523, 1240
BbvCI CCTCAGC 1 cut(s) 546
BccI CCATC 6 cut(s) 461, 761, 956, 1085, 1249, 1346
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 3 cut(s) 695, 1118, 1275
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 3 cut(s) 454, 494, 725
BfmI CTRYAG 4 cut(s) 185, 1142, 1206, 1212
BfoI RGCGCY 1 cut(s) 305
BglII AGATCT 1 cut(s) 384
Bme1390I CCNGG 4 cut(s) 10, 695, 1118, 1275
Bme18I GGWCC 2 cut(s) 112, 1271
BmeT110I CYCGRG 2 cut(s) 176, 633
BmgT120I GGNCC 3 cut(s) 112, 1064, 1271
BmiI GGNNCC 3 cut(s) 1066, 1272, 1279
BmrFI CCNGG 4 cut(s) 10, 695, 1118, 1275
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 3 cut(s) 3, 77, 262
BmuI ACTGGG 1 cut(s) 616
BoxI GACNNNNGTC 2 cut(s) 154, 737
BpiI GAAGAC 1 cut(s) 698
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 3 cut(s) 51, 160, 1116
BsaWI WCCGGW 2 cut(s) 38, 235
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 463, 1147
Bse1I ACTGG 3 cut(s) 528, 611, 1014
Bse3DI GCAATG 2 cut(s) 621, 944
BseBI CCWGG 3 cut(s) 695, 1118, 1275
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 51, 160, 1116
BseGI GGATG 7 cut(s) 18, 453, 753, 871, 967, 1096, 1174
BseLI CCNNNNNNNGG 3 cut(s) 16, 463, 1147
BseMI GCAATG 2 cut(s) 621, 944
BseMII CTCAG 2 cut(s) 446, 537
BseNI ACTGG 3 cut(s) 528, 611, 1014
BseRI GAGGAG 3 cut(s) 482, 563, 1163
Bsh1236I CGCG 2 cut(s) 1200, 1234
BshFI GGCC 4 cut(s) 165, 851, 1065, 1333
BshVI ATCGAT 1 cut(s) 71
BsiHKAI GWGCWC 3 cut(s) 433, 523, 1240
BsiHKCI CYCGRG 2 cut(s) 176, 633
BsiSI CCGG 4 cut(s) 10, 39, 123, 236
BslFI GGGAC 5 cut(s) 142, 417, 651, 1284, 1354
BslI CCNNNNNNNGG 3 cut(s) 16, 463, 1147
BsmAI GTCTC 3 cut(s) 454, 494, 725
BsmFI GGGAC 5 cut(s) 142, 417, 651, 1284, 1354
BsnI GGCC 4 cut(s) 165, 851, 1065, 1333
Bso31I GGTCTC 1 cut(s) 725
BsoBI CYCGRG 2 cut(s) 176, 633
Bsp1286I GDGCHC 4 cut(s) 433, 523, 1240, 1282
Bsp143I GATC 1 cut(s) 384
BspACI CCGC 3 cut(s) 480, 777, 1249
BspANI GGCC 4 cut(s) 165, 851, 1065, 1333
BspCNI CTCAG 2 cut(s) 445, 538
BspDI ATCGAT 1 cut(s) 71
BspFNI CGCG 2 cut(s) 1200, 1234
BspLI GGNNCC 3 cut(s) 1066, 1272, 1279
BspMAI CTGCAG 3 cut(s) 1146, 1210, 1216
BspTNI GGTCTC 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 621, 944
BsrI ACTGG 3 cut(s) 528, 611, 1014
BssECI CCNNGG 3 cut(s) 51, 160, 1116
BssMI GATC 1 cut(s) 384
Bst2UI CCWGG 3 cut(s) 695, 1118, 1275
Bst4CI ACNGT 2 cut(s) 715, 792
Bst6I CTCTTC 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 259
BstC8I GCNNGC 3 cut(s) 429, 919, 1236
BstDEI CTNAG 4 cut(s) 432, 546, 583, 1287
BstENI CCTNNNNNAGG 1 cut(s) 1145
BstF5I GGATG 7 cut(s) 18, 453, 753, 871, 967, 1096, 1174
BstFNI CGCG 2 cut(s) 1200, 1234
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 2 cut(s) 304, 1234
BstKTI GATC 1 cut(s) 387
BstMAI GTCTC 3 cut(s) 454, 494, 725
BstMBI GATC 1 cut(s) 384
BstMWI GCNNNNNNNGC 8 cut(s) 256, 259, 299, 551, 955, 1211, 1214, 1217
BstNI CCWGG 3 cut(s) 695, 1118, 1275
BstPAI GACNNNNGTC 2 cut(s) 154, 737
BstSCI CCNGG 4 cut(s) 8, 693, 1116, 1273
BstSFI CTRYAG 4 cut(s) 185, 1142, 1206, 1212
BstUI CGCG 2 cut(s) 1200, 1234
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
Bsu15I ATCGAT 1 cut(s) 71
BsuRI GGCC 4 cut(s) 165, 851, 1065, 1333
BsuTUI ATCGAT 1 cut(s) 71
BtsCI GGATG 7 cut(s) 18, 453, 753, 871, 967, 1096, 1174
BtsI GCAGTG 1 cut(s) 1244
BtsIMutI CAGTG 6 cut(s) 521, 711, 748, 788, 1233, 1244
Cac8I GCNNGC 3 cut(s) 429, 919, 1236
CfoI GCGC 2 cut(s) 304, 1234
Cfr13I GGNCC 3 cut(s) 112, 1064, 1271
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 4 cut(s) 198, 716, 992, 1184
CviAII CATG 4 cut(s) 154, 494, 922, 977
CviQI GTAC 4 cut(s) 198, 716, 992, 1184
DdeI CTNAG 4 cut(s) 432, 546, 583, 1287
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Ecl136II GAGCTC 2 cut(s) 431, 1238
Eco24I GRGCYC 3 cut(s) 433, 1240, 1282
Eco31I GGTCTC 1 cut(s) 725
Eco47I GGWCC 2 cut(s) 112, 1271
Eco47III AGCGCT 1 cut(s) 303
Eco53kI GAGCTC 2 cut(s) 431, 1238
Eco88I CYCGRG 2 cut(s) 176, 633
EcoICRI GAGCTC 2 cut(s) 431, 1238
EcoNI CCTNNNNNAGG 1 cut(s) 1145
EcoRI GAATTC 1 cut(s) 667
EcoRII CCWGG 3 cut(s) 693, 1116, 1273
EcoT38I GRGCYC 3 cut(s) 433, 1240, 1282
FaeI CATG 4 cut(s) 157, 497, 925, 980
FaqI GGGAC 5 cut(s) 142, 417, 651, 1284, 1354
FatI CATG 4 cut(s) 153, 493, 921, 976
FauI CCCGC 1 cut(s) 784
FauNDI CATATG 1 cut(s) 831
FblI GTMKAC 1 cut(s) 171
FokI GGATG 7 cut(s) 25, 440, 740, 858, 974, 1103, 1181
FriOI GRGCYC 3 cut(s) 433, 1240, 1282
GlaI GCGC 2 cut(s) 303, 1233
GsuI CTGGAG 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 4 cut(s) 165, 851, 1065, 1333
HapII CCGG 4 cut(s) 10, 39, 123, 236
HhaI GCGC 2 cut(s) 304, 1234
Hin1II CATG 4 cut(s) 157, 497, 925, 980
Hin6I GCGC 2 cut(s) 302, 1232
HinP1I GCGC 2 cut(s) 302, 1232
HincII GTYRAC 4 cut(s) 172, 988, 1180, 1264
HindII GTYRAC 4 cut(s) 172, 988, 1180, 1264
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 6 cut(s) 47, 150, 181, 569, 762, 926
HpaII CCGG 4 cut(s) 10, 39, 123, 236
HphI GGTGA 4 cut(s) 494, 709, 1105, 1106
Hpy166II GTNNAC 5 cut(s) 172, 485, 988, 1180, 1264
Hpy188I TCNGA 5 cut(s) 24, 52, 246, 380, 1078
Hpy188III TCNNGA 2 cut(s) 44, 178
Hpy8I GTNNAC 5 cut(s) 172, 485, 988, 1180, 1264
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 4 cut(s) 73, 586, 896, 1080
HpyCH4III ACNGT 2 cut(s) 715, 792
HpyCH4IV ACGT 2 cut(s) 990, 1182
HpyCH4V TGCA 4 cut(s) 1144, 1208, 1214, 1328
HpyF10VI GCNNNNNNNGC 8 cut(s) 256, 259, 299, 551, 955, 1211, 1214, 1217
HpyF3I CTNAG 4 cut(s) 432, 546, 583, 1287
HpySE526I ACGT 2 cut(s) 990, 1182
Hsp92II CATG 4 cut(s) 157, 497, 925, 980
HspAI GCGC 2 cut(s) 302, 1232
Kzo9I GATC 1 cut(s) 384
LmnI GCTCC 4 cut(s) 290, 518, 1243, 1277
LweI GCATC 3 cut(s) 3, 77, 262
MaeII ACGT 2 cut(s) 990, 1182
MaeIII GTNAC 3 cut(s) 265, 358, 1294
MalI GATC 1 cut(s) 386
MboI GATC 1 cut(s) 384
MboII GAAGA 5 cut(s) 109, 698, 1133, 1145, 1315
MfeI CAATTG 1 cut(s) 1059
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 4 cut(s) 433, 523, 1240, 1282
MluCI AATT 7 cut(s) 348, 510, 592, 667, 912, 1059, 1319
MluI ACGCGT 1 cut(s) 1198
MlyI GAGTC 2 cut(s) 144, 920
MmeI TCCRAC 2 cut(s) 582, 1030
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 4 cut(s) 140, 437, 530, 1256
MslI CAYNNNNRTG 2 cut(s) 1016, 1044
MspA1I CMGCKG 3 cut(s) 259, 1211, 1217
MspI CCGG 4 cut(s) 10, 39, 123, 236
MspR9I CCNGG 4 cut(s) 10, 695, 1118, 1275
MunI CAATTG 1 cut(s) 1059
MvaI CCWGG 3 cut(s) 695, 1118, 1275
MvnI CGCG 2 cut(s) 1200, 1234
MwoI GCNNNNNNNGC 8 cut(s) 256, 259, 299, 551, 955, 1211, 1214, 1217
NciI CCSGG 1 cut(s) 10
NdeI CATATG 1 cut(s) 831
NdeII GATC 1 cut(s) 384
NlaIII CATG 4 cut(s) 157, 497, 925, 980
NlaIV GGNNCC 3 cut(s) 1066, 1272, 1279
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 265, 358
PaeR7I CTCGAG 1 cut(s) 176
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 174
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 4 cut(s) 47, 181, 569, 762
PflFI GACNNNGTC 1 cut(s) 642
PleI GAGTC 2 cut(s) 144, 920
PpsI GAGTC 2 cut(s) 144, 920
PshAI GACNNNNGTC 2 cut(s) 154, 737
Psp124BI GAGCTC 2 cut(s) 433, 1240
Psp6I CCWGG 3 cut(s) 693, 1116, 1273
PspGI CCWGG 3 cut(s) 693, 1116, 1273
PspN4I GGNNCC 3 cut(s) 1066, 1272, 1279
PspPI GGNCC 3 cut(s) 112, 1064, 1271
PstI CTGCAG 3 cut(s) 1146, 1210, 1216
PsuI RGATCY 1 cut(s) 384
PsyI GACNNNGTC 1 cut(s) 642
PvuII CAGCTG 3 cut(s) 259, 1211, 1217
RsaI GTAC 4 cut(s) 199, 717, 993, 1185
RsaNI GTAC 4 cut(s) 198, 716, 992, 1184
RseI CAYNNNNRTG 2 cut(s) 1016, 1044
SacI GAGCTC 2 cut(s) 433, 1240
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 4 cut(s) 140, 437, 530, 1256
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 3 cut(s) 112, 1064, 1271
SbfI CCTGCAGG 1 cut(s) 1146
SchI GAGTC 2 cut(s) 144, 920
ScrFI CCNGG 4 cut(s) 10, 695, 1118, 1275
SdaI CCTGCAGG 1 cut(s) 1146
SduI GDGCHC 4 cut(s) 433, 523, 1240, 1282
SfaNI GCATC 3 cut(s) 3, 77, 262
SfcI CTRYAG 4 cut(s) 185, 1142, 1206, 1212
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 2 cut(s) 112, 1271
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 2 cut(s) 1016, 1044
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse8387I CCTGCAGG 1 cut(s) 1146
Sse9I AATT 7 cut(s) 348, 510, 592, 667, 912, 1059, 1319
SsiI CCGC 3 cut(s) 480, 777, 1249
SspI AATATT 1 cut(s) 679
SstI GAGCTC 2 cut(s) 433, 1240
StyD4I CCNGG 4 cut(s) 8, 693, 1116, 1273
TaaI ACNGT 2 cut(s) 715, 792
TaiI ACGT 2 cut(s) 993, 1185
TaqI TCGA 5 cut(s) 71, 171, 177, 646, 1158
TasI AATT 7 cut(s) 348, 510, 592, 667, 912, 1059, 1319
TfiI GAWTC 4 cut(s) 47, 181, 569, 762
Tru1I TTAA 4 cut(s) 140, 437, 530, 1256
Tru9I TTAA 4 cut(s) 140, 437, 530, 1256
TscAI CASTG 6 cut(s) 528, 718, 748, 795, 1233, 1251
TseFI GTSAC 2 cut(s) 265, 358
Tsp45I GTSAC 2 cut(s) 265, 358
TspDTI ATGAA 7 cut(s) 426, 915, 1034, 1176, 1185, 1212, 1332
TspGWI ACGGA 3 cut(s) 746, 903, 1181
TspRI CASTG 6 cut(s) 528, 718, 748, 795, 1233, 1251
Tth111I GACNNNGTC 1 cut(s) 642
VpaK11BI GGWCC 2 cut(s) 112, 1271
XagI CCTNNNNNAGG 1 cut(s) 1145
XapI RAATTY 1 cut(s) 667
XhoI CTCGAG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.