Rw6G028830

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002295
Physical Location & Seq
Forward (+)
52886914 .. 52890407
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028830.1

Sequence Viewer

Length: 1437 bp
ATGATGACGTGGAACGGCGCCCGCGACATAGTGCTCGACTCGGATGACGTTCCGTTCGGGACCGGTGAGTGGTACCTCTACGCCGGAATCTCTTGCGTGCTGGTTTTGTTCGCCGGAATCATGTCCGGCCTTACGCTCGGATTGATGTCGCTCAATCTCGTCGACCTCGAGATTCTCCAGCGGAGCGGTTCCACCGATCAGAAAAAGCAAGCCGCTGCGATTCTACCTGTGGTGAAAAAGCAGCACCAGCTTTTGGTCACTTTGCTTCTCTGCAATGCTTGTGCCATGGAGGCCCTTCCTTTATACCTTGATGAAATCTTTCACCCTGTTGTTGCCGTTTTGCTGTCTGTAACATTTGTTCTAATTTTCGGAGAGATAATTCCACAAGCTATATGCTCAAGATATGGGCTGTCTGTTGGTTCAAACTTCGTTTGGCTTGTTCGCATTCTGATGATCATCTGCTATCCAATCGCATACCCAATTGGAAAGGTTCTGGATCTTGTTCTCGGACACGGGGATGATTTGTTCAGGCGAGCACAGTTGAAAGCTCTCGTCTCTATTCACGGCCTTGAGGCTGGAAAAGGAGGTGAACTAACACATGATGAGACAACGATCATCAGTGGAGCGCTAGATTTAACTGAAAAGACTGCTGAGGAGGCTATGACACCTATTGAATCAACATTTTCCTTGGATGTTAATTCAAAGTTAGACTGGGAAGCAATTGGGAAAATTCTTGCACGTGGTCATAGTAGAGTCCCTGTCTATTCTGGAAATCCCAAAAACATAATCGGCCTCTTACTGGTGAAAAGTCTCCTCACCGTACGAGCAGAAACAGAGACTCCAGTTAGTGCAGTTTCCATTCGTAGAATGCCTAGGGTTCCAGCCGATATGCCTTTGTATGATATACTTAATGAGTTTCAAAAAGGAAGCAGTCACCTGGCTGCTGTAGTGAAGATCAAAGGAAAAACCAAGAATCCTCTACTTTTTGCTGATAGAGAGAAACTCGAGGAGGCCAGACTAACCAATGGATGTGTTGCCATTGATGTCGATAAACGTCCAAGGTCTCTGACAAACATGCCAACTGTTCAAGAAAATGATGCTACTACAAATGGCATTTCAAATTCATCAGATGATATTGAAGATGGGGAGGTGATTGGAATCATAACCCTGGAGGATGTTTTTGAAGAACTTCTGCAAGAAGAAATTGTAGATGAGACAGATGTATATATTGATGTACATAAGAGGATACGTGTGGCTGCTGCTGCAGCTGCTTCATCCATGTCCAGAGCTCAGTCAAGTCAGAAGTTGATTAGCCAAAAGCATGCAGGAGCTACAAATAAGCACGGACAAACCCCGAAGAAGTCTGGGGAGGATGATGCACATTCGACGAGGTTTTCAGGGAGTTCACGGGAGCCTCTTCTAGGTGTTATAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
DUF21

Protein Analysis

478

Amino Acids

52.05

Weight (kDa)

5.47

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 30 - 191 3.7e-34 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 2 cut(s) 17, 72
AccB7I CCANNNNNTGG 1 cut(s) 253
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 162
AccII CGCG 1 cut(s) 24
AciI CCGC 4 cut(s) 22, 181, 186, 213
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 2 cut(s) 729, 1120
AcvI CACGTG 1 cut(s) 740
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 74, 822, 1236
AfeI AGCGCT 1 cut(s) 627
AfiI CCNNNNNNNGG 4 cut(s) 69, 253, 799, 1421
AflIII ACRYGT 1 cut(s) 1249
AgeI ACCGGT 1 cut(s) 62
AgsI TTSAA 9 cut(s) 423, 544, 674, 702, 920, 1088, 1119, 1139, 1184
AjiI CACGTC 1 cut(s) 9
AjnI CCWGG 2 cut(s) 936, 1167
AloI GAACNNNNNNTCC 2 cut(s) 509, 541
AluBI AGCT 6 cut(s) 250, 389, 548, 1268, 1289, 1331
AluI AGCT 6 cut(s) 250, 389, 548, 1268, 1289, 1331
Alw21I GWGCWC 3 cut(s) 36, 538, 1291
Alw26I GTCTC 6 cut(s) 559, 599, 815, 830, 1068, 1208
AlwI GGATC 1 cut(s) 504
Ama87I CYCGRG 2 cut(s) 167, 1004
Aor51HI AGCGCT 1 cut(s) 627
AoxI GGCC 5 cut(s) 127, 291, 565, 790, 1011
ApeKI GCWGC 8 cut(s) 215, 241, 941, 1256, 1259, 1262, 1265, 1268
ApoI RAATTY 2 cut(s) 729, 1120
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AsiGI ACCGGT 1 cut(s) 62
Asp700I GAANNNNTTC 2 cut(s) 318, 1188
Asp718I GGTACC 1 cut(s) 72
AspA2I CCTAGG 1 cut(s) 872
AspLEI GCGC 2 cut(s) 20, 628
AspS9I GGNCC 2 cut(s) 60, 292
AsuHPI GGTGA 8 cut(s) 77, 244, 314, 599, 808, 814, 926, 1162
AvaI CYCGRG 2 cut(s) 167, 1004
AvaII GGWCC 1 cut(s) 60
AvrII CCTAGG 1 cut(s) 872
BanI GGYRCC 2 cut(s) 17, 72
BanII GRGCYC 1 cut(s) 1291
BbrPI CACGTG 1 cut(s) 740
Bbv12I GWGCWC 3 cut(s) 36, 538, 1291
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 8 cut(s) 202, 253, 928, 1243, 1246, 1249, 1255, 1277
BccI CCATC 1 cut(s) 1136
BceAI ACGGC 3 cut(s) 31, 320, 580
BciT130I CCWGG 2 cut(s) 938, 1169
BciVI GTATCC 1 cut(s) 1239
BclI TGATCA 1 cut(s) 453
BcoDI GTCTC 6 cut(s) 559, 599, 815, 830, 1068, 1208
BfaI CTAG 3 cut(s) 629, 873, 1421
BfmI CTRYAG 2 cut(s) 945, 1263
BfoI RGCGCY 2 cut(s) 21, 629
BfuI GTATCC 1 cut(s) 1239
BglI GCCNNNNNGGC 1 cut(s) 290
BisI GCNGC 9 cut(s) 213, 216, 242, 942, 1257, 1260, 1263, 1266, 1269
BlnI CCTAGG 1 cut(s) 872
BlsI GCNGC 9 cut(s) 214, 217, 243, 943, 1258, 1261, 1264, 1267, 1270
Bme1390I CCNGG 2 cut(s) 938, 1169
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 2 cut(s) 167, 1004
BmgBI CACGTC 1 cut(s) 9
BmgT120I GGNCC 2 cut(s) 60, 292
BmiI GGNNCC 6 cut(s) 19, 61, 74, 190, 879, 1413
BmrFI CCNGG 2 cut(s) 938, 1169
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 2 cut(s) 1087, 1366
BmuI ACTGGG 1 cut(s) 721
BplI GAGNNNNNCTC 2 cut(s) 987, 1019
BpmI CTGGAG 3 cut(s) 161, 825, 1190
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 2 cut(s) 382, 590
BsaAI YACGTR 2 cut(s) 740, 1250
BsaBI GATNNNNATC 2 cut(s) 455, 1157
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 1068
BsaJI CCNNGG 5 cut(s) 285, 687, 872, 1058, 1167
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 823, 853
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 253, 799, 1421
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 3 cut(s) 716, 804, 842
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 2 cut(s) 455, 1157
BseBI CCWGG 2 cut(s) 938, 1169
BseDI CCNNGG 5 cut(s) 285, 687, 872, 1058, 1167
BseGI GGATG 7 cut(s) 49, 523, 697, 1034, 1180, 1274, 1378
BseJI GATNNNNATC 2 cut(s) 455, 1157
BseLI CCNNNNNNNGG 4 cut(s) 69, 253, 799, 1421
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 642, 1304
BseNI ACTGG 3 cut(s) 716, 804, 842
BseRI GAGGAG 3 cut(s) 668, 803, 1022
BseXI GCAGC 8 cut(s) 202, 253, 928, 1243, 1246, 1249, 1255, 1277
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 5 cut(s) 129, 293, 567, 792, 1013
BshNI GGYRCC 2 cut(s) 17, 72
BshTI ACCGGT 1 cut(s) 62
BsiHKAI GWGCWC 3 cut(s) 36, 538, 1291
BsiHKCI CYCGRG 2 cut(s) 167, 1004
BsiSI CCGG 4 cut(s) 63, 84, 114, 126
BsiWI CGTACG 1 cut(s) 820
BslFI GGGAC 2 cut(s) 73, 740
BslI CCNNNNNNNGG 4 cut(s) 69, 253, 799, 1421
BsmAI GTCTC 6 cut(s) 559, 599, 815, 830, 1068, 1208
BsmBI CGTCTC 1 cut(s) 559
BsmFI GGGAC 2 cut(s) 73, 740
BsmI GAATGC 2 cut(s) 444, 873
BsnI GGCC 5 cut(s) 129, 293, 567, 792, 1013
Bso31I GGTCTC 1 cut(s) 1068
BsoBI CYCGRG 2 cut(s) 167, 1004
Bsp1286I GDGCHC 3 cut(s) 36, 538, 1291
Bsp1407I TGTACA 1 cut(s) 1234
Bsp143I GATC 5 cut(s) 196, 453, 496, 612, 954
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 4 cut(s) 22, 181, 186, 213
BspANI GGCC 5 cut(s) 129, 293, 567, 792, 1013
BspCNI CTCAG 2 cut(s) 643, 1303
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 6 cut(s) 19, 61, 74, 190, 879, 1413
BspMAI CTGCAG 1 cut(s) 1267
BspPI GGATC 1 cut(s) 504
BspT107I GGYRCC 2 cut(s) 17, 72
BspTNI GGTCTC 1 cut(s) 1068
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 1234
BsrI ACTGG 3 cut(s) 716, 804, 842
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 5 cut(s) 285, 687, 872, 1058, 1167
BssMI GATC 5 cut(s) 196, 453, 496, 612, 954
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 4 cut(s) 285, 687, 872, 1058
Bst2UI CCWGG 2 cut(s) 938, 1169
Bst4CI ACNGT 3 cut(s) 540, 820, 1084
Bst6I CTCTTC 1 cut(s) 1422
BstACI GRCGYC 1 cut(s) 18
BstAUI TGTACA 1 cut(s) 1234
BstBAI YACGTR 2 cut(s) 740, 1250
BstC8I GCNNGC 5 cut(s) 22, 98, 210, 534, 1323
BstDEI CTNAG 2 cut(s) 651, 1290
BstDSI CCRYGG 1 cut(s) 285
BstENI CCTNNNNNAGG 1 cut(s) 1419
BstF5I GGATG 7 cut(s) 49, 523, 697, 1034, 1180, 1274, 1378
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 2 cut(s) 21, 629
BstHHI GCGC 2 cut(s) 20, 628
BstKTI GATC 5 cut(s) 199, 456, 499, 615, 957
BstMAI GTCTC 6 cut(s) 559, 599, 815, 830, 1068, 1208
BstMBI GATC 5 cut(s) 196, 453, 496, 612, 954
BstMWI GCNNNNNNNGC 6 cut(s) 247, 290, 656, 1262, 1265, 1268
BstNI CCWGG 2 cut(s) 938, 1169
BstNSI RCATGY 2 cut(s) 1078, 1325
BstSCI CCNGG 2 cut(s) 936, 1167
BstSFI CTRYAG 2 cut(s) 945, 1263
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 8 cut(s) 202, 253, 928, 1243, 1246, 1249, 1255, 1277
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuI GTATCC 1 cut(s) 1239
BsuRI GGCC 5 cut(s) 129, 293, 567, 792, 1013
BtgI CCRYGG 1 cut(s) 285
BtrI CACGTC 1 cut(s) 9
BtsCI GGATG 7 cut(s) 49, 523, 697, 1034, 1180, 1274, 1378
BtsIMutI CAGTG 1 cut(s) 625
Cac8I GCNNGC 5 cut(s) 22, 98, 210, 534, 1323
CfoI GCGC 2 cut(s) 20, 628
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 60, 292
Csp6I GTAC 3 cut(s) 73, 821, 1235
CspAI ACCGGT 1 cut(s) 62
CviAII CATG 6 cut(s) 121, 286, 599, 1075, 1279, 1322
CviQI GTAC 3 cut(s) 73, 821, 1235
DdeI CTNAG 2 cut(s) 651, 1290
DinI GGCGCC 1 cut(s) 19
DpnI GATC 5 cut(s) 198, 455, 498, 614, 956
DpnII GATC 5 cut(s) 196, 453, 496, 612, 954
Eam1104I CTCTTC 1 cut(s) 1422
EarI CTCTTC 1 cut(s) 1422
Ecl136II GAGCTC 1 cut(s) 1289
Eco130I CCWWGG 4 cut(s) 285, 687, 872, 1058
Eco24I GRGCYC 1 cut(s) 1291
Eco31I GGTCTC 1 cut(s) 1068
Eco47I GGWCC 1 cut(s) 60
Eco47III AGCGCT 1 cut(s) 627
Eco53kI GAGCTC 1 cut(s) 1289
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 2 cut(s) 167, 1004
EcoICRI GAGCTC 1 cut(s) 1289
EcoNI CCTNNNNNAGG 1 cut(s) 1419
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 936, 1167
EcoT14I CCWWGG 4 cut(s) 285, 687, 872, 1058
EcoT38I GRGCYC 1 cut(s) 1291
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
ErhI CCWWGG 4 cut(s) 285, 687, 872, 1058
Esp3I CGTCTC 1 cut(s) 559
FaeI CATG 6 cut(s) 124, 289, 602, 1078, 1282, 1325
FaqI GGGAC 2 cut(s) 73, 740
FatI CATG 6 cut(s) 120, 285, 598, 1074, 1278, 1321
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 1 cut(s) 453
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 9 cut(s) 213, 216, 242, 942, 1257, 1260, 1263, 1266, 1269
FokI GGATG 7 cut(s) 56, 530, 704, 1041, 1187, 1261, 1385
FriOI GRGCYC 1 cut(s) 1291
Fsp4HI GCNGC 9 cut(s) 213, 216, 242, 942, 1257, 1260, 1263, 1266, 1269
FspBI CTAG 3 cut(s) 629, 873, 1421
GlaI GCGC 2 cut(s) 19, 627
GluI GCNGC 9 cut(s) 213, 216, 242, 942, 1257, 1260, 1263, 1266, 1269
GsuI CTGGAG 3 cut(s) 161, 825, 1190
HaeII RGCGCY 2 cut(s) 21, 629
HaeIII GGCC 5 cut(s) 129, 293, 567, 792, 1013
HapII CCGG 4 cut(s) 63, 84, 114, 126
HhaI GCGC 2 cut(s) 20, 628
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 6 cut(s) 124, 289, 602, 1078, 1282, 1325
Hin6I GCGC 2 cut(s) 18, 626
HinP1I GCGC 2 cut(s) 18, 626
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HpaII CCGG 4 cut(s) 63, 84, 114, 126
HphI GGTGA 8 cut(s) 77, 244, 314, 599, 808, 814, 926, 1162
Hpy166II GTNNAC 3 cut(s) 163, 590, 1406
Hpy188I TCNGA 9 cut(s) 43, 140, 201, 371, 450, 509, 1068, 1129, 1302
Hpy188III TCNNGA 7 cut(s) 58, 169, 399, 494, 768, 1088, 1284
Hpy8I GTNNAC 3 cut(s) 163, 590, 1406
Hpy99I CGWCG 2 cut(s) 164, 1390
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 3 cut(s) 540, 820, 1084
HpyCH4IV ACGT 5 cut(s) 8, 48, 739, 1054, 1249
HpyCH4V TGCA 7 cut(s) 273, 737, 851, 1195, 1265, 1325, 1379
HpyF10VI GCNNNNNNNGC 6 cut(s) 247, 290, 656, 1262, 1265, 1268
HpyF3I CTNAG 2 cut(s) 651, 1290
HpySE526I ACGT 5 cut(s) 8, 48, 739, 1054, 1249
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 6 cut(s) 124, 289, 602, 1078, 1282, 1325
HspAI GCGC 2 cut(s) 18, 626
KasI GGCGCC 1 cut(s) 17
KpnI GGTACC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 453
Kzo9I GATC 5 cut(s) 196, 453, 496, 612, 954
LmnI GCTCC 4 cut(s) 183, 623, 1328, 1411
Lsp1109I GCAGC 8 cut(s) 202, 253, 928, 1243, 1246, 1249, 1255, 1277
LweI GCATC 2 cut(s) 1087, 1366
MaeI CTAG 3 cut(s) 629, 873, 1421
MaeII ACGT 5 cut(s) 8, 48, 739, 1054, 1249
MaeIII GTNAC 3 cut(s) 256, 349, 932
MalI GATC 5 cut(s) 198, 455, 498, 614, 956
MbiI CCGCTC 1 cut(s) 186
MboI GATC 5 cut(s) 196, 453, 496, 612, 954
MboII GAAGA 6 cut(s) 964, 1151, 1196, 1211, 1369, 1409
MfeI CAATTG 2 cut(s) 480, 720
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 3 cut(s) 36, 538, 1291
MluCI AATT 8 cut(s) 363, 378, 480, 697, 720, 729, 1120, 1203
Mly113I GGCGCC 1 cut(s) 18
MlyI GAGTC 3 cut(s) 32, 762, 832
MroXI GAANNNNTTC 2 cut(s) 318, 1188
MseI TTAA 3 cut(s) 635, 696, 909
MslI CAYNNNNRTG 2 cut(s) 449, 516
MspA1I CMGCKG 3 cut(s) 181, 215, 1268
MspI CCGG 4 cut(s) 63, 84, 114, 126
MspR9I CCNGG 2 cut(s) 938, 1169
MunI CAATTG 2 cut(s) 480, 720
Mva1269I GAATGC 2 cut(s) 444, 873
MvaI CCWGG 2 cut(s) 938, 1169
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 6 cut(s) 247, 290, 656, 1262, 1265, 1268
NarI GGCGCC 1 cut(s) 18
NcoI CCATGG 1 cut(s) 285
NdeII GATC 5 cut(s) 196, 453, 496, 612, 954
NlaIII CATG 6 cut(s) 124, 289, 602, 1078, 1282, 1325
NlaIV GGNNCC 6 cut(s) 19, 61, 74, 190, 879, 1413
NmuCI GTSAC 2 cut(s) 256, 932
NspI RCATGY 2 cut(s) 1078, 1325
PaeI GCATGC 1 cut(s) 1325
PaeR7I CTCGAG 2 cut(s) 167, 1004
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 165
PctI GAATGC 2 cut(s) 444, 873
PdmI GAANNNNTTC 2 cut(s) 318, 1188
PfeI GAWTC 7 cut(s) 87, 117, 172, 220, 674, 973, 1158
Pfl23II CGTACG 1 cut(s) 820
PflMI CCANNNNNTGG 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 62
PkrI GCNGC 9 cut(s) 214, 217, 243, 943, 1258, 1261, 1264, 1267, 1270
PleI GAGTC 3 cut(s) 32, 761, 832
PluTI GGCGCC 1 cut(s) 21
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 3 cut(s) 32, 761, 832
Ppu21I YACGTR 2 cut(s) 740, 1250
PsiI TTATAA 1 cut(s) 1430
Psp124BI GAGCTC 1 cut(s) 1291
Psp6I CCWGG 2 cut(s) 936, 1167
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 2 cut(s) 936, 1167
PspLI CGTACG 1 cut(s) 820
PspN4I GGNNCC 6 cut(s) 19, 61, 74, 190, 879, 1413
PspPI GGNCC 2 cut(s) 60, 292
PspXI VCTCGAGB 1 cut(s) 1004
PsrI GAACNNNNNNTAC 2 cut(s) 342, 374
PstI CTGCAG 1 cut(s) 1267
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 1268
RsaI GTAC 3 cut(s) 74, 822, 1236
RsaNI GTAC 3 cut(s) 73, 821, 1235
RseI CAYNNNNRTG 2 cut(s) 449, 516
SacI GAGCTC 1 cut(s) 1291
SalI GTCGAC 1 cut(s) 161
SaqAI TTAA 3 cut(s) 635, 696, 909
SatI GCNGC 9 cut(s) 213, 216, 242, 942, 1257, 1260, 1263, 1266, 1269
Sau3AI GATC 5 cut(s) 196, 453, 496, 612, 954
Sau96I GGNCC 2 cut(s) 60, 292
SchI GAGTC 3 cut(s) 32, 762, 832
ScrFI CCNGG 2 cut(s) 938, 1169
SduI GDGCHC 3 cut(s) 36, 538, 1291
SfaNI GCATC 2 cut(s) 1087, 1366
SfcI CTRYAG 2 cut(s) 945, 1263
SfoI GGCGCC 1 cut(s) 19
Sfr274I CTCGAG 2 cut(s) 167, 1004
SinI GGWCC 1 cut(s) 60
SlaI CTCGAG 2 cut(s) 167, 1004
SmiMI CAYNNNNRTG 2 cut(s) 449, 516
SmlI CTYRAG 4 cut(s) 167, 397, 569, 1004
SmoI CTYRAG 4 cut(s) 167, 397, 569, 1004
SphI GCATGC 1 cut(s) 1325
Sse9I AATT 8 cut(s) 363, 378, 480, 697, 720, 729, 1120, 1203
SsiI CCGC 4 cut(s) 22, 181, 186, 213
SspDI GGCGCC 1 cut(s) 17
SspMI CTAG 3 cut(s) 629, 873, 1421
SstI GAGCTC 1 cut(s) 1291
StyD4I CCNGG 2 cut(s) 936, 1167
StyI CCWWGG 4 cut(s) 285, 687, 872, 1058
TaaI ACNGT 3 cut(s) 540, 820, 1084
TaiI ACGT 5 cut(s) 11, 51, 742, 1057, 1252
TaqI TCGA 6 cut(s) 36, 162, 168, 1005, 1047, 1385
TasI AATT 8 cut(s) 363, 378, 480, 697, 720, 729, 1120, 1203
TatI WGTACW 1 cut(s) 1234
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 7 cut(s) 87, 117, 172, 220, 674, 973, 1158
Tru1I TTAA 3 cut(s) 635, 696, 909
Tru9I TTAA 3 cut(s) 635, 696, 909
TscAI CASTG 1 cut(s) 625
TseFI GTSAC 2 cut(s) 256, 932
TseI GCWGC 8 cut(s) 215, 241, 941, 1256, 1259, 1262, 1265, 1268
Tsp45I GTSAC 2 cut(s) 256, 932
TspDTI ATGAA 3 cut(s) 327, 1113, 1263
TspGWI ACGGA 2 cut(s) 42, 1359
TspRI CASTG 1 cut(s) 625
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 60
XagI CCTNNNNNAGG 1 cut(s) 1419
XapI RAATTY 2 cut(s) 729, 1120
XceI RCATGY 2 cut(s) 1078, 1325
XhoI CTCGAG 2 cut(s) 167, 1004
XmaJI CCTAGG 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 2 cut(s) 318, 1188
XspI CTAG 3 cut(s) 629, 873, 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.