Rh6DG332100

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002295
Physical Location & Seq
Forward (+)
53743260 .. 53745770
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG332100.1

Sequence Viewer

Length: 936 bp
ATGATGACGTGGAACGGAGCCCGCGACATAGTGCTCGACTCGGATGACGTTCCGTTCGGGACCGGTGAATGGTACCTCTACGCCGGAATCTCTTGCGTGCTGGTTTTGTTCGCCGGAATCATGTCCGGCCTTACGCTCGGATTGATGTCGCTCAATCTCGTCGACCTCGAGATTCTCCAGCGGAGCGGTTCCTCCGATCAGAAAAAGCAAGCCGCTGCGATTCTACCTGTGGTGAAAAAGCAGCACCAGCTTTTGGTCACTTTGCTTCTCTGCAATGCTTGTGCCATGGAGGCCCTTCCTTTATACCTTGATGAAATCTTTCACCCTGTTGTTGCCGTTTTGCTGTCTGTAACATTTGTTCTAATTTTCGGAGAGATTATTCCACAAGCTATATGCTCAAGATATGGGCTGTCTGTTGGTTCAAACTTCGTTTGGCTTGTTCGCATTCTGATGATCATCTGCTATCCAATCGCGTACCCAATTGGAAAGGTTCTGGATCTTGTTCTCGGACACGGGGATGATTTGTTCAGGCGAGCACAGTTGAAAGCCCTCGTCTCTATTCACGGCCTTGAGGCTGGAAAAGGAGGTGAACTAACACATGATGAGACAACGATCATCAGTGGAGCGCTAGATTTAACTGAAAAGACTGCTGAGGAGGCTATGACACCTATTGAATCAACATTTTCCTTGGATGTTAATTCAAAGTTAGACTGGGAAGCAATTGGGAAAATTCTTGCACGTGGTCATAGTAGAGTCCCTGTCTATTCTGGAAATCCCAAAAACATAATCGGCCTCTTACTGGTGAAAAGTCTCCTCACAGTACGAGCAGAAACAGAGACTCCAGTTAGTGCAGTTTCCATTCGTAGAATGCCTAGGTTTATCTTTTTCTTCCTCTTCTCTTCTCTTCTATATCTGTTCTCTTTAGGAAAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
DUF21

Protein Analysis

311

Amino Acids

34.09

Weight (kDa)

5.98

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 30 - 191 1.2e-34 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 72
AccB7I CCANNNNNTGG 1 cut(s) 253
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 162
AccII CGCG 2 cut(s) 24, 473
AciI CCGC 4 cut(s) 22, 181, 186, 213
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 1 cut(s) 729
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 3 cut(s) 74, 476, 822
AfeI AGCGCT 1 cut(s) 627
AfiI CCNNNNNNNGG 3 cut(s) 69, 253, 799
AgeI ACCGGT 1 cut(s) 62
AgsI TTSAA 4 cut(s) 423, 544, 674, 702
AjiI CACGTC 1 cut(s) 9
AloI GAACNNNNNNTCC 2 cut(s) 509, 541
AluBI AGCT 2 cut(s) 250, 389
AluI AGCT 2 cut(s) 250, 389
Alw21I GWGCWC 2 cut(s) 36, 538
Alw26I GTCTC 4 cut(s) 559, 599, 815, 830
AlwI GGATC 1 cut(s) 504
Ama87I CYCGRG 1 cut(s) 167
Aor51HI AGCGCT 1 cut(s) 627
AoxI GGCC 4 cut(s) 127, 291, 565, 790
ApeKI GCWGC 2 cut(s) 215, 241
ApoI RAATTY 1 cut(s) 729
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AsiGI ACCGGT 1 cut(s) 62
Asp700I GAANNNNTTC 1 cut(s) 318
Asp718I GGTACC 1 cut(s) 72
AspA2I CCTAGG 1 cut(s) 872
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 2 cut(s) 60, 292
AsuHPI GGTGA 5 cut(s) 77, 244, 314, 599, 814
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 60
AvrII CCTAGG 1 cut(s) 872
BanI GGYRCC 1 cut(s) 72
BanII GRGCYC 1 cut(s) 22
BbrPI CACGTG 1 cut(s) 740
Bbv12I GWGCWC 2 cut(s) 36, 538
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 2 cut(s) 202, 253
BceAI ACGGC 2 cut(s) 320, 580
BclI TGATCA 1 cut(s) 453
BcoDI GTCTC 4 cut(s) 559, 599, 815, 830
BfaI CTAG 2 cut(s) 629, 873
BfoI RGCGCY 1 cut(s) 629
BglI GCCNNNNNGGC 1 cut(s) 290
BisI GCNGC 3 cut(s) 213, 216, 242
BlnI CCTAGG 1 cut(s) 872
BlsI GCNGC 3 cut(s) 214, 217, 243
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 167
BmgBI CACGTC 1 cut(s) 9
BmgT120I GGNCC 2 cut(s) 60, 292
BmiI GGNNCC 4 cut(s) 19, 61, 74, 190
BmrI ACTGGG 1 cut(s) 721
BmuI ACTGGG 1 cut(s) 721
BpmI CTGGAG 2 cut(s) 161, 825
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 2 cut(s) 382, 590
BsaAI YACGTR 1 cut(s) 740
BsaBI GATNNNNATC 1 cut(s) 455
BsaJI CCNNGG 3 cut(s) 285, 687, 872
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 823, 853
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 253, 799
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 3 cut(s) 716, 804, 842
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 1 cut(s) 455
BseDI CCNNGG 3 cut(s) 285, 687, 872
BseGI GGATG 3 cut(s) 49, 523, 697
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 3 cut(s) 69, 253, 799
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 1 cut(s) 642
BseNI ACTGG 3 cut(s) 716, 804, 842
BseRI GAGGAG 2 cut(s) 668, 803
BseXI GCAGC 2 cut(s) 202, 253
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 2 cut(s) 24, 473
BshFI GGCC 4 cut(s) 129, 293, 567, 792
BshNI GGYRCC 1 cut(s) 72
BshTI ACCGGT 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 36, 538
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 4 cut(s) 63, 84, 114, 126
BslFI GGGAC 2 cut(s) 73, 740
BslI CCNNNNNNNGG 3 cut(s) 69, 253, 799
BsmAI GTCTC 4 cut(s) 559, 599, 815, 830
BsmBI CGTCTC 1 cut(s) 559
BsmFI GGGAC 2 cut(s) 73, 740
BsmI GAATGC 2 cut(s) 444, 873
BsnI GGCC 4 cut(s) 129, 293, 567, 792
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 3 cut(s) 22, 36, 538
Bsp143I GATC 4 cut(s) 196, 453, 496, 612
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 4 cut(s) 22, 181, 186, 213
BspANI GGCC 4 cut(s) 129, 293, 567, 792
BspCNI CTCAG 1 cut(s) 643
BspFNI CGCG 2 cut(s) 24, 473
BspLI GGNNCC 4 cut(s) 19, 61, 74, 190
BspPI GGATC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 3 cut(s) 716, 804, 842
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 3 cut(s) 285, 687, 872
BssMI GATC 4 cut(s) 196, 453, 496, 612
BssT1I CCWWGG 3 cut(s) 285, 687, 872
Bst4CI ACNGT 2 cut(s) 540, 820
Bst6I CTCTTC 3 cut(s) 899, 904, 909
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 4 cut(s) 22, 98, 210, 534
BstDEI CTNAG 1 cut(s) 651
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 3 cut(s) 49, 523, 697
BstFNI CGCG 2 cut(s) 24, 473
BstH2I RGCGCY 1 cut(s) 629
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 4 cut(s) 199, 456, 499, 615
BstMAI GTCTC 4 cut(s) 559, 599, 815, 830
BstMBI GATC 4 cut(s) 196, 453, 496, 612
BstMWI GCNNNNNNNGC 3 cut(s) 247, 290, 656
BstUI CGCG 2 cut(s) 24, 473
BstV1I GCAGC 2 cut(s) 202, 253
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuRI GGCC 4 cut(s) 129, 293, 567, 792
BtgI CCRYGG 1 cut(s) 285
BtrI CACGTC 1 cut(s) 9
BtsCI GGATG 3 cut(s) 49, 523, 697
BtsIMutI CAGTG 1 cut(s) 625
Cac8I GCNNGC 4 cut(s) 22, 98, 210, 534
CfoI GCGC 1 cut(s) 628
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 60, 292
Csp6I GTAC 3 cut(s) 73, 475, 821
CspAI ACCGGT 1 cut(s) 62
CviAII CATG 3 cut(s) 121, 286, 599
CviQI GTAC 3 cut(s) 73, 475, 821
DdeI CTNAG 1 cut(s) 651
DpnI GATC 4 cut(s) 198, 455, 498, 614
DpnII GATC 4 cut(s) 196, 453, 496, 612
Eam1104I CTCTTC 3 cut(s) 899, 904, 909
EarI CTCTTC 3 cut(s) 899, 904, 909
Eco130I CCWWGG 3 cut(s) 285, 687, 872
Eco24I GRGCYC 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 60
Eco47III AGCGCT 1 cut(s) 627
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 292
EcoT14I CCWWGG 3 cut(s) 285, 687, 872
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 3 cut(s) 285, 687, 872
Esp3I CGTCTC 1 cut(s) 559
FaeI CATG 3 cut(s) 124, 289, 602
FaqI GGGAC 2 cut(s) 73, 740
FatI CATG 3 cut(s) 120, 285, 598
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 1 cut(s) 453
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 213, 216, 242
FokI GGATG 3 cut(s) 56, 530, 704
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 3 cut(s) 213, 216, 242
FspBI CTAG 2 cut(s) 629, 873
GlaI GCGC 1 cut(s) 627
GluI GCNGC 3 cut(s) 213, 216, 242
GsuI CTGGAG 2 cut(s) 161, 825
HaeII RGCGCY 1 cut(s) 629
HaeIII GGCC 4 cut(s) 129, 293, 567, 792
HapII CCGG 4 cut(s) 63, 84, 114, 126
HhaI GCGC 1 cut(s) 628
Hin1II CATG 3 cut(s) 124, 289, 602
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 8 cut(s) 38, 87, 117, 172, 220, 674, 753, 838
HpaII CCGG 4 cut(s) 63, 84, 114, 126
HphI GGTGA 5 cut(s) 77, 244, 314, 599, 814
Hpy166II GTNNAC 2 cut(s) 163, 590
Hpy188I TCNGA 7 cut(s) 43, 140, 196, 201, 371, 450, 509
Hpy188III TCNNGA 5 cut(s) 58, 169, 399, 494, 768
Hpy8I GTNNAC 2 cut(s) 163, 590
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 2 cut(s) 540, 820
HpyCH4IV ACGT 3 cut(s) 8, 48, 739
HpyCH4V TGCA 3 cut(s) 273, 737, 851
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 290, 656
HpyF3I CTNAG 1 cut(s) 651
HpySE526I ACGT 3 cut(s) 8, 48, 739
Hsp92II CATG 3 cut(s) 124, 289, 602
HspAI GCGC 1 cut(s) 626
KpnI GGTACC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 453
Kzo9I GATC 4 cut(s) 196, 453, 496, 612
LmnI GCTCC 3 cut(s) 17, 183, 623
Lsp1109I GCAGC 2 cut(s) 202, 253
MaeI CTAG 2 cut(s) 629, 873
MaeII ACGT 3 cut(s) 8, 48, 739
MaeIII GTNAC 2 cut(s) 256, 349
MalI GATC 4 cut(s) 198, 455, 498, 614
MbiI CCGCTC 1 cut(s) 186
MboI GATC 4 cut(s) 196, 453, 496, 612
MboII GAAGA 4 cut(s) 880, 886, 891, 896
MfeI CAATTG 2 cut(s) 480, 720
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 3 cut(s) 22, 36, 538
MluCI AATT 5 cut(s) 363, 480, 697, 720, 729
MlyI GAGTC 3 cut(s) 32, 762, 832
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 3 cut(s) 635, 696, 934
MslI CAYNNNNRTG 2 cut(s) 449, 516
MspA1I CMGCKG 2 cut(s) 181, 215
MspI CCGG 4 cut(s) 63, 84, 114, 126
MunI CAATTG 2 cut(s) 480, 720
Mva1269I GAATGC 2 cut(s) 444, 873
MvnI CGCG 2 cut(s) 24, 473
MwoI GCNNNNNNNGC 3 cut(s) 247, 290, 656
NcoI CCATGG 1 cut(s) 285
NdeII GATC 4 cut(s) 196, 453, 496, 612
NlaIII CATG 3 cut(s) 124, 289, 602
NlaIV GGNNCC 4 cut(s) 19, 61, 74, 190
NmuCI GTSAC 1 cut(s) 256
PaeR7I CTCGAG 1 cut(s) 167
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 165
PctI GAATGC 2 cut(s) 444, 873
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 5 cut(s) 87, 117, 172, 220, 674
PflMI CCANNNNNTGG 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 62
PkrI GCNGC 3 cut(s) 214, 217, 243
PleI GAGTC 3 cut(s) 32, 761, 832
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 3 cut(s) 32, 761, 832
Ppu21I YACGTR 1 cut(s) 740
PspCI CACGTG 1 cut(s) 740
PspN4I GGNNCC 4 cut(s) 19, 61, 74, 190
PspPI GGNCC 2 cut(s) 60, 292
PsrI GAACNNNNNNTAC 2 cut(s) 342, 374
PsuI RGATCY 1 cut(s) 496
RsaI GTAC 3 cut(s) 74, 476, 822
RsaNI GTAC 3 cut(s) 73, 475, 821
RseI CAYNNNNRTG 2 cut(s) 449, 516
SalI GTCGAC 1 cut(s) 161
SaqAI TTAA 3 cut(s) 635, 696, 934
SatI GCNGC 3 cut(s) 213, 216, 242
Sau3AI GATC 4 cut(s) 196, 453, 496, 612
Sau96I GGNCC 2 cut(s) 60, 292
SchI GAGTC 3 cut(s) 32, 762, 832
SduI GDGCHC 3 cut(s) 22, 36, 538
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 60
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 2 cut(s) 449, 516
SmlI CTYRAG 3 cut(s) 167, 397, 569
SmoI CTYRAG 3 cut(s) 167, 397, 569
Sse9I AATT 5 cut(s) 363, 480, 697, 720, 729
SsiI CCGC 4 cut(s) 22, 181, 186, 213
SspMI CTAG 2 cut(s) 629, 873
StyI CCWWGG 3 cut(s) 285, 687, 872
TaaI ACNGT 2 cut(s) 540, 820
TaiI ACGT 3 cut(s) 11, 51, 742
TaqI TCGA 3 cut(s) 36, 162, 168
TasI AATT 5 cut(s) 363, 480, 697, 720, 729
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 5 cut(s) 87, 117, 172, 220, 674
Tru1I TTAA 3 cut(s) 635, 696, 934
Tru9I TTAA 3 cut(s) 635, 696, 934
TscAI CASTG 1 cut(s) 625
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 2 cut(s) 215, 241
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 327
TspGWI ACGGA 2 cut(s) 30, 42
TspRI CASTG 1 cut(s) 625
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 1 cut(s) 729
XhoI CTCGAG 1 cut(s) 167
XmaJI CCTAGG 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 2 cut(s) 629, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.