MD13G1228100.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004892
Physical Location & Seq
Reverse (-)
22342374 .. 22342817
444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1228100.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGCATATATCCCACATGAAAAAATGAGTCCATGTTTAACAAATGCTCTTCAATTCATGCTGCTTCTACTAGTTGTTCTAACAACATCAGTTGAGGCTGGTCTACTTTCTAAAGTAACTATTCAAATCACAAACGCATTGGGACCGAACACAGACCTCACACTTCATTGCAAGTCCAAGGACGATGATCTCGGCTATCACAGACTTCATCCCGGAGATAGTTTTGCGTTTAAGTTTAAGCCAAACATTTGGGGGACTACACTGTTTTTTTGCAGCTTTGAGTGGCCAGGAGAATTTCATTACTATGATGTCTTCAGAGCAGATAGGGATGGTTGTGGAAAATGTTATTGGACTGTCTATTTTCTTGGCATTTGTTCTCATGATCACAAGTGTATTCAATGGCAGGATCGGGCCGGTTCTAACCCTAGGCCGTTAATTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

148

Amino Acids

16.81

Weight (kDa)

6.63

Isoelectric Point (pI)

20.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 40 - 131 3.6e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 103
AclWI GGATC 1 cut(s) 414
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 298
AgsI TTSAA 3 cut(s) 53, 125, 398
AhlI ACTAGT 1 cut(s) 70
AjnI CCWGG 1 cut(s) 286
AjuI GAANNNNNNNTTGG 2 cut(s) 331, 363
AluBI AGCT 1 cut(s) 276
AluI AGCT 1 cut(s) 276
AlwI GGATC 1 cut(s) 414
AoxI GGCC 3 cut(s) 284, 411, 428
ApeKI GCWGC 2 cut(s) 61, 273
ApoI RAATTY 1 cut(s) 293
AspA2I CCTAGG 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 143, 411
AsuC2I CCSGG 1 cut(s) 213
AvaII GGWCC 1 cut(s) 143
AvrII CCTAGG 1 cut(s) 425
BalI TGGCCA 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 48, 285
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 415
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 213
BcuI ACTAGT 1 cut(s) 70
BfaI CTAG 2 cut(s) 71, 426
BisI GCNGC 2 cut(s) 62, 274
BlnI CCTAGG 1 cut(s) 425
BlsI GCNGC 2 cut(s) 63, 275
Bme1390I CCNGG 2 cut(s) 213, 288
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 2 cut(s) 143, 411
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 2 cut(s) 213, 288
BpiI GAAGAC 1 cut(s) 304
BpuMI CCSGG 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 177, 425
Bse118I RCCGGY 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 166
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 177, 425
BseGI GGATG 2 cut(s) 208, 334
BseMI GCAATG 1 cut(s) 166
BseXI GCAGC 2 cut(s) 48, 285
BshFI GGCC 3 cut(s) 286, 413, 430
BsiSI CCGG 2 cut(s) 213, 414
BslFI GGGAC 2 cut(s) 156, 268
BsmFI GGGAC 2 cut(s) 156, 268
BsnI GGCC 3 cut(s) 286, 413, 430
Bsp143I GATC 3 cut(s) 187, 382, 406
BspANI GGCC 3 cut(s) 286, 413, 430
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 144
BspPI GGATC 1 cut(s) 414
BspQI GCTCTTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 177, 425
BssMI GATC 3 cut(s) 187, 382, 406
BssT1I CCWWGG 2 cut(s) 177, 425
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 264, 355
Bst6I CTCTTC 1 cut(s) 54
BstF5I GGATG 2 cut(s) 208, 334
BstKTI GATC 3 cut(s) 190, 385, 409
BstMBI GATC 3 cut(s) 187, 382, 406
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 211, 286
BstV1I GCAGC 2 cut(s) 48, 285
BstV2I GAAGAC 1 cut(s) 304
BstXI CCANNNNNNTGG 1 cut(s) 249
BsuRI GGCC 3 cut(s) 286, 413, 430
BtsCI GGATG 2 cut(s) 208, 334
BtsIMutI CAGTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 2 cut(s) 143, 411
CviAII CATG 4 cut(s) 17, 33, 58, 380
CviJI RGCY 7 cut(s) 98, 195, 241, 276, 286, 413, 430
CviKI_1 RGCY 7 cut(s) 98, 195, 241, 276, 286, 413, 430
DpnI GATC 3 cut(s) 189, 384, 408
DpnII GATC 3 cut(s) 187, 382, 406
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 177, 425
Eco47I GGWCC 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 177, 425
ErhI CCWWGG 2 cut(s) 177, 425
FaeI CATG 4 cut(s) 20, 36, 61, 383
FaiI YATR 8 cut(s) 7, 9, 18, 34, 59, 306, 381, 442
FaqI GGGAC 2 cut(s) 156, 268
FatI CATG 4 cut(s) 16, 32, 57, 379
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 2 cut(s) 62, 274
FokI GGATG 2 cut(s) 195, 341
Fsp4HI GCNGC 2 cut(s) 62, 274
FspBI CTAG 2 cut(s) 71, 426
GluI GCNGC 2 cut(s) 62, 274
HaeIII GGCC 3 cut(s) 286, 413, 430
HapII CCGG 2 cut(s) 213, 414
Hin1II CATG 4 cut(s) 20, 36, 61, 383
HinfI GANTC 1 cut(s) 28
HpaII CCGG 2 cut(s) 213, 414
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 1 cut(s) 380
Hpy8I GTNNAC 1 cut(s) 104
HpyCH4III ACNGT 2 cut(s) 264, 355
HpyCH4V TGCA 2 cut(s) 171, 273
Hsp92II CATG 4 cut(s) 20, 36, 61, 383
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 187, 382, 406
LguI GCTCTTC 1 cut(s) 54
LpnPI CCDG 6 cut(s) 84, 226, 273, 300, 389, 427
Lsp1109I GCAGC 2 cut(s) 48, 285
MaeI CTAG 2 cut(s) 71, 426
MaeIII GTNAC 1 cut(s) 115
MalI GATC 3 cut(s) 189, 384, 408
MboI GATC 3 cut(s) 187, 382, 406
MboII GAAGA 2 cut(s) 41, 304
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 3 cut(s) 53, 293, 435
MluNI TGGCCA 1 cut(s) 286
MlyI GAGTC 1 cut(s) 37
MnlI CCTC 2 cut(s) 88, 167
Mox20I TGGCCA 1 cut(s) 286
MscI TGGCCA 1 cut(s) 286
MseI TTAA 4 cut(s) 38, 231, 237, 434
MslI CAYNNNNRTG 1 cut(s) 303
Msp20I TGGCCA 1 cut(s) 286
MspI CCGG 2 cut(s) 213, 414
MspR9I CCNGG 2 cut(s) 213, 288
MvaI CCWGG 1 cut(s) 288
NciI CCSGG 1 cut(s) 213
NdeII GATC 3 cut(s) 187, 382, 406
NlaIII CATG 4 cut(s) 20, 36, 61, 383
NlaIV GGNNCC 1 cut(s) 144
NmeAIII GCCGAG 1 cut(s) 171
PagI TCATGA 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 54
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 2 cut(s) 63, 275
PleI GAGTC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 2 cut(s) 143, 411
RseI CAYNNNNRTG 1 cut(s) 303
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 4 cut(s) 38, 231, 237, 434
SatI GCNGC 2 cut(s) 62, 274
Sau3AI GATC 3 cut(s) 187, 382, 406
Sau96I GGNCC 2 cut(s) 143, 411
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 2 cut(s) 213, 288
SetI ASST 2 cut(s) 159, 278
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 303
SpeI ACTAGT 1 cut(s) 70
Sse9I AATT 3 cut(s) 53, 293, 435
SspMI CTAG 2 cut(s) 71, 426
StyD4I CCNGG 2 cut(s) 211, 286
StyI CCWWGG 2 cut(s) 177, 425
TaaI ACNGT 2 cut(s) 264, 355
TaqII GACCGA 1 cut(s) 160
TasI AATT 3 cut(s) 53, 293, 435
Tru1I TTAA 4 cut(s) 38, 231, 237, 434
Tru9I TTAA 4 cut(s) 38, 231, 237, 434
TscAI CASTG 1 cut(s) 267
TseI GCWGC 2 cut(s) 61, 273
TspDTI ATGAA 5 cut(s) 33, 46, 155, 197, 287
TspRI CASTG 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 1 cut(s) 293
XmaJI CCTAGG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 103
XspI CTAG 2 cut(s) 71, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.