Rh7DG294000

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004892
Physical Location & Seq
Forward (+)
33267243 .. 33271530
4288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG294000.1

Sequence Viewer

Length: 627 bp
ATGTCGTTTTATGAGAATGAGGGCTTATGCGGCGTCGTTTTGGAGAAGCCGTGTGCGGCGAATACTGTAACAGCTGGAGCTATTGAGGTTGACGGTCACGAGCAGCTGAAGAAGACAGCTAGTGCGATTTTGTGGATCATAGCGGCGACGTTCAGGATATGGCTATTTGTCCTCGTCGTCAGGACGAGCAAAATGAGTCCTCTATCGAAAACTATGATTTCCCTTGTGCTGTTGTTCTGCATCCTCACAACCACCATTGAGCACACACATGCTCTTTCGTTTTGCAAAATATTTCGATGCCCAAAGGACACCACCGTTAGAGTAACCAACCGTTTGGAAAGCAATGAGGTCCTGACCGTCCACTGTAAATCGAAAGATGATGACATCGGTGAACAAAAACTTGTGTTTGATAACGACTTTCAATTCAAGTTTAAGACAGATTTTGGCATAGTGGATAGCAGGACATTATTCTTTTGTAGCTTTCAATGGGGGAATGAATTCAAAAGGTTCGATATTTTCATTGCTAACAGGGATCGTTGCACTGAATGCTTTTGGGACGTCTATGAAAGAGGCCCGTGTTTGGAATATAACCAAGGTCAATTTGGTTATTGCCTACCGTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

208

Amino Acids

24.01

Weight (kDa)

6.43

Isoelectric Point (pI)

27.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 105 - 207 5.2e-26 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 561
AciI CCGC 3 cut(s) 30, 56, 143
AclWI GGATC 2 cut(s) 143, 540
AcsI RAATTY 1 cut(s) 497
AcuI CTGAAG 1 cut(s) 128
AcyI GRCGYC 2 cut(s) 33, 558
AfiI CCNNNNNNNGG 1 cut(s) 580
AgsI TTSAA 4 cut(s) 422, 427, 485, 502
AluBI AGCT 5 cut(s) 74, 80, 106, 119, 480
AluI AGCT 5 cut(s) 74, 80, 106, 119, 480
Alw21I GWGCWC 1 cut(s) 264
AlwI GGATC 2 cut(s) 143, 540
AoxI GGCC 1 cut(s) 571
ApeKI GCWGC 1 cut(s) 103
ApoI RAATTY 1 cut(s) 497
Asp700I GAANNNNTTC 1 cut(s) 497
AspS9I GGNCC 2 cut(s) 349, 572
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 349
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 119
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 115
BceAI ACGGC 1 cut(s) 34
BfaI CTAG 1 cut(s) 120
BisI GCNGC 4 cut(s) 31, 57, 104, 144
BlsI GCNGC 4 cut(s) 32, 58, 105, 145
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 2 cut(s) 349, 572
BmsI GCATC 2 cut(s) 249, 287
BpiI GAAGAC 1 cut(s) 119
BpmI CTGGAG 1 cut(s) 96
BsaHI GRCGYC 2 cut(s) 33, 558
BsaJI CCNNGG 2 cut(s) 592, 617
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse3DI GCAATG 2 cut(s) 349, 519
BseDI CCNNGG 2 cut(s) 592, 617
BseGI GGATG 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMI GCAATG 2 cut(s) 349, 519
BseXI GCAGC 1 cut(s) 115
BshFI GGCC 1 cut(s) 573
BsiHKAI GWGCWC 1 cut(s) 264
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 1 cut(s) 580
BsmFI GGGAC 1 cut(s) 569
BsmI GAATGC 1 cut(s) 551
BsnI GGCC 1 cut(s) 573
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 2 cut(s) 135, 532
BspACI CCGC 3 cut(s) 30, 56, 143
BspANI GGCC 1 cut(s) 573
BspPI GGATC 2 cut(s) 143, 540
BsrDI GCAATG 2 cut(s) 349, 519
BssECI CCNNGG 2 cut(s) 592, 617
BssMI GATC 2 cut(s) 135, 532
BssNI GRCGYC 2 cut(s) 33, 558
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 592
Bst2BI CACGAG 1 cut(s) 98
Bst4CI ACNGT 7 cut(s) 67, 95, 316, 332, 358, 365, 618
BstACI GRCGYC 2 cut(s) 33, 558
BstAPI GCANNNNNTGC 1 cut(s) 546
BstDSI CCRYGG 1 cut(s) 617
BstF5I GGATG 1 cut(s) 240
BstKTI GATC 2 cut(s) 138, 535
BstMBI GATC 2 cut(s) 135, 532
BstMWI GCNNNNNNNGC 2 cut(s) 30, 546
BstNSI RCATGY 1 cut(s) 272
BstV1I GCAGC 1 cut(s) 115
BstV2I GAAGAC 1 cut(s) 119
BstXI CCANNNNNNTGG 1 cut(s) 334
BsuRI GGCC 1 cut(s) 573
BtgI CCRYGG 1 cut(s) 617
BtsCI GGATG 1 cut(s) 240
BtsIMutI CAGTG 2 cut(s) 361, 540
Cfr13I GGNCC 2 cut(s) 349, 572
CseI GACGC 1 cut(s) 22
CviAII CATG 1 cut(s) 269
CviJI RGCY 9 cut(s) 24, 49, 74, 80, 106, 119, 163, 480, 573
CviKI_1 RGCY 9 cut(s) 24, 49, 74, 80, 106, 119, 163, 480, 573
DpnI GATC 2 cut(s) 137, 534
DpnII GATC 2 cut(s) 135, 532
Eco130I CCWWGG 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 592
FaeI CATG 1 cut(s) 272
FaiI YATR 9 cut(s) 12, 28, 140, 160, 215, 270, 449, 564, 588
FaqI GGGAC 1 cut(s) 569
FatI CATG 1 cut(s) 268
Fnu4HI GCNGC 4 cut(s) 31, 57, 104, 144
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 4 cut(s) 31, 57, 104, 144
FspBI CTAG 1 cut(s) 120
GluI GCNGC 4 cut(s) 31, 57, 104, 144
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 1 cut(s) 573
HgaI GACGC 1 cut(s) 22
Hin1I GRCGYC 2 cut(s) 33, 558
Hin1II CATG 1 cut(s) 272
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 1 cut(s) 196
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 3 cut(s) 91, 361, 392
Hpy188III TCNNGA 4 cut(s) 98, 154, 181, 352
Hpy8I GTNNAC 3 cut(s) 91, 361, 392
Hpy99I CGWCG 3 cut(s) 38, 151, 179
HpyCH4III ACNGT 7 cut(s) 67, 95, 316, 332, 358, 365, 618
HpyCH4IV ACGT 2 cut(s) 149, 558
HpyCH4V TGCA 3 cut(s) 240, 285, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 546
HpySE526I ACGT 2 cut(s) 149, 558
Hsp92I GRCGYC 2 cut(s) 33, 558
Hsp92II CATG 1 cut(s) 272
Kzo9I GATC 2 cut(s) 135, 532
LmnI GCTCC 1 cut(s) 77
LpnPI CCDG 6 cut(s) 60, 139, 166, 365, 445, 514
Lsp1109I GCAGC 1 cut(s) 115
LweI GCATC 2 cut(s) 249, 287
MaeI CTAG 1 cut(s) 120
MaeII ACGT 2 cut(s) 149, 558
MaeIII GTNAC 3 cut(s) 67, 95, 322
MalI GATC 2 cut(s) 137, 534
MboI GATC 2 cut(s) 135, 532
MboII GAAGA 2 cut(s) 121, 124
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 3 cut(s) 422, 497, 599
MlyI GAGTC 1 cut(s) 205
MnlI CCTC 7 cut(s) 13, 79, 182, 210, 254, 340, 563
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 1 cut(s) 267
MspA1I CMGCKG 2 cut(s) 74, 106
Mva1269I GAATGC 1 cut(s) 551
MwoI GCNNNNNNNGC 2 cut(s) 30, 546
NdeII GATC 2 cut(s) 135, 532
NlaIII CATG 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 272
PctI GAATGC 1 cut(s) 551
PdmI GAANNNNTTC 1 cut(s) 497
PkrI GCNGC 4 cut(s) 32, 58, 105, 145
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
PpuMI RGGWCCY 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 349
PspPI GGNCC 2 cut(s) 349, 572
PspPPI RGGWCCY 1 cut(s) 349
PvuII CAGCTG 2 cut(s) 74, 106
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 4 cut(s) 31, 57, 104, 144
Sau3AI GATC 2 cut(s) 135, 532
Sau96I GGNCC 2 cut(s) 349, 572
SchI GAGTC 1 cut(s) 205
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 2 cut(s) 249, 287
SinI GGWCC 1 cut(s) 349
SmiMI CAYNNNNRTG 1 cut(s) 267
Sse9I AATT 3 cut(s) 422, 497, 599
SsiI CCGC 3 cut(s) 30, 56, 143
SspI AATATT 1 cut(s) 291
SspMI CTAG 1 cut(s) 120
StyI CCWWGG 1 cut(s) 592
TaaI ACNGT 7 cut(s) 67, 95, 316, 332, 358, 365, 618
TaiI ACGT 2 cut(s) 152, 561
TaqI TCGA 4 cut(s) 206, 295, 371, 510
TasI AATT 3 cut(s) 422, 497, 599
TauI GCSGC 3 cut(s) 33, 59, 146
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 2 cut(s) 368, 547
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 508, 510, 579
TspRI CASTG 2 cut(s) 368, 547
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 1 cut(s) 497
XceI RCATGY 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 120
ZraI GACGTC 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.