MD13G1228300.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004892
Physical Location & Seq
Reverse (-)
22357771 .. 22358181
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1228300.v1.1.491

Sequence Viewer

Length: 411 bp
ATGAGTCCATCTTTTACTGCAACATGTGCTCTTCCGCTGATGCTGATGCTGCTAGTTGTTTATGTAACAATAGTTGCCGAGGCGTCTTACCCTCTTAAGAAAATAACGGTTCGAATCAACAATACTTTGGAAAACAACCAGGAACTTACAGTTCACTGTAAATCAGGGAACGATGATCTAGGCCCTCACAACCTCCTTCCAAAGAACACATATGAATTTAGTTTTAAACGAAACTTTTTCCAGCCGAAAACGCTATTCTTTTGCACCTTCCAATGGCCAGAGGATCAATTTAAACTTCACTATTTCGACATCTACGACCAAAAAAGGGATGACTGCAGCAAGTGTTTCTGGGAGATTTATCAATTTGGCGCTTGTTTGTATGGTAACTGCAAAAGGTGGAATAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

137

Amino Acids

16.05

Weight (kDa)

7.55

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 36 - 129 1.9e-24 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 35
AclWI GGATC 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 273, 325
AflII CTTAAG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 23
AjnI CCWGG 1 cut(s) 138
Alw21I GWGCWC 1 cut(s) 31
AlwI GGATC 1 cut(s) 291
AoxI GGCC 2 cut(s) 181, 275
ApeKI GCWGC 2 cut(s) 49, 336
ApoI RAATTY 1 cut(s) 215
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 1 cut(s) 182
AsuII TTCGAA 1 cut(s) 112
BalI TGGCCA 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 2 cut(s) 36, 348
BccI CCATC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 140
BfaI CTAG 2 cut(s) 53, 179
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 372
BfrI CTTAAG 1 cut(s) 95
BisI GCNGC 2 cut(s) 50, 337
BlsI GCNGC 2 cut(s) 51, 338
Bme1390I CCNGG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 30, 36
Bpu14I TTCGAA 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 2 cut(s) 273, 325
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 2 cut(s) 273, 325
BseXI GCAGC 2 cut(s) 36, 348
BshFI GGCC 2 cut(s) 183, 277
BsiHKAI GWGCWC 1 cut(s) 31
BslI CCNNNNNNNGG 2 cut(s) 273, 325
BsnI GGCC 2 cut(s) 183, 277
Bsp119I TTCGAA 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 2 cut(s) 175, 283
BspACI CCGC 1 cut(s) 35
BspANI GGCC 2 cut(s) 183, 277
BspHI TCATGA 1 cut(s) 407
BspMAI CTGCAG 1 cut(s) 338
BspPI GGATC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 112
BspTI CTTAAG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 2 cut(s) 175, 283
BssNI GRCGYC 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 109, 151, 158
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 83
BstAFI CTTAAG 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 112
BstF5I GGATG 1 cut(s) 334
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 2 cut(s) 178, 286
BstMBI GATC 2 cut(s) 175, 283
BstMWI GCNNNNNNNGC 3 cut(s) 26, 49, 250
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 36, 348
BsuRI GGCC 2 cut(s) 183, 277
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 154
CciI TCATGA 1 cut(s) 407
CfoI GCGC 1 cut(s) 371
Cfr13I GGNCC 1 cut(s) 182
CseI GACGC 1 cut(s) 72
CviAII CATG 2 cut(s) 24, 408
CviJI RGCY 3 cut(s) 183, 244, 277
CviKI_1 RGCY 3 cut(s) 183, 244, 277
DpnI GATC 2 cut(s) 177, 285
DpnII GATC 2 cut(s) 175, 283
DraI TTTAAA 2 cut(s) 226, 292
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 138
FaeI CATG 2 cut(s) 27, 411
FaiI YATR 6 cut(s) 25, 63, 211, 213, 381, 409
FatI CATG 2 cut(s) 23, 407
FauNDI CATATG 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 50, 337
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 2 cut(s) 50, 337
FspBI CTAG 2 cut(s) 53, 179
GlaI GCGC 1 cut(s) 370
GluI GCNGC 2 cut(s) 50, 337
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 2 cut(s) 183, 277
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 371
Hin1I GRCGYC 1 cut(s) 83
Hin1II CATG 2 cut(s) 27, 411
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HinfI GANTC 2 cut(s) 4, 114
Hpy166II GTNNAC 1 cut(s) 154
Hpy188III TCNNGA 1 cut(s) 408
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 206, 277
HpyCH4III ACNGT 3 cut(s) 109, 151, 158
HpyCH4V TGCA 4 cut(s) 20, 264, 336, 390
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 49, 250
Hsp92I GRCGYC 1 cut(s) 83
Hsp92II CATG 2 cut(s) 27, 411
HspAI GCGC 1 cut(s) 369
Kzo9I GATC 2 cut(s) 175, 283
LguI GCTCTTC 1 cut(s) 36
LpnPI CCDG 6 cut(s) 125, 150, 152, 254, 291, 334
Lsp1109I GCAGC 2 cut(s) 36, 348
LweI GCATC 2 cut(s) 30, 36
MaeI CTAG 2 cut(s) 53, 179
MaeIII GTNAC 2 cut(s) 64, 383
MalI GATC 2 cut(s) 177, 285
MboI GATC 2 cut(s) 175, 283
MboII GAAGA 1 cut(s) 23
MhlI GDGCHC 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 277
MluCI AATT 4 cut(s) 215, 287, 362, 403
MluNI TGGCCA 1 cut(s) 277
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 5 cut(s) 73, 102, 195, 203, 274
Mox20I TGGCCA 1 cut(s) 277
MscI TGGCCA 1 cut(s) 277
MseI TTAA 3 cut(s) 96, 225, 291
Msp20I TGGCCA 1 cut(s) 277
MspA1I CMGCKG 1 cut(s) 37
MspCI CTTAAG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 26, 49, 250
NdeI CATATG 1 cut(s) 211
NdeII GATC 2 cut(s) 175, 283
NlaIII CATG 2 cut(s) 27, 411
NmeAIII GCCGAG 1 cut(s) 103
NspI RCATGY 1 cut(s) 27
NspV TTCGAA 1 cut(s) 112
PagI TCATGA 1 cut(s) 407
PciI ACATGT 1 cut(s) 23
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 1 cut(s) 114
PkrI GCNGC 2 cut(s) 51, 338
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspPI GGNCC 1 cut(s) 182
PstI CTGCAG 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 3 cut(s) 96, 225, 291
SatI GCNGC 2 cut(s) 50, 337
Sau3AI GATC 2 cut(s) 175, 283
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 31
SetI ASST 3 cut(s) 195, 269, 398
SfaNI GCATC 2 cut(s) 30, 36
SfcI CTRYAG 1 cut(s) 334
SfuI TTCGAA 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 4 cut(s) 215, 287, 362, 403
SsiI CCGC 1 cut(s) 35
SspMI CTAG 2 cut(s) 53, 179
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 3 cut(s) 109, 151, 158
TaqI TCGA 2 cut(s) 112, 306
TasI AATT 4 cut(s) 215, 287, 362, 403
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 3 cut(s) 96, 225, 291
Tru9I TTAA 3 cut(s) 96, 225, 291
TscAI CASTG 1 cut(s) 161
TseI GCWGC 2 cut(s) 49, 336
TspDTI ATGAA 2 cut(s) 228, 396
TspRI CASTG 1 cut(s) 161
Vha464I CTTAAG 1 cut(s) 95
XapI RAATTY 1 cut(s) 215
XceI RCATGY 1 cut(s) 27
XspI CTAG 2 cut(s) 53, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.