MD15G1364700.v1.1

Phosphoglycerate mutase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001245
Physical Location & Seq
Forward (+)
44184265 .. 44188016
3752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1364700.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGAAAACCAAATGGCTGCTGCTGCTGCTGCATTTCACCAAAGCATTGGGACTCCTCACTCTGTTGGGCATGTTAATAATTCCGGCCTGCTTCAAGAGCTTGCGAAAAGCTCAGTGAGTTTCATTTCAAAGGGTTTTGAGATTGATATTGGACTGTCAAAAAGAGGATGCTACATCTCTTCCAAGAGAAACGTTAGTGTAATTCGAGCCTCAGCTTCCCAGGCTTCAGTGATTGATATAGTTTCATCTCCCTCACATACCAAAGCCAATGATTCTCGGAAAAAGTCTAATGAAGCAGCTTTGATTTTGATTAGGCATGGTGAGTCACTGTGGAACGAAAAGAACTTGTTCACAGGCTGTGTTGATGTCCCTCTGAGCAAGAAGGGTGTAGAAGAGGCAATTGAAGCTGGCAAGAGAATCAGCAACATACCTGTCGACATGATCTATACATCTGCACTGATTCGTGCACAGATGACTGCCATGCTTGCCATGACACAGCACCGTCGGAAGAAGGTCCCAGTTATAATGCATAACGAGAGTGAACGGGCTAGGGCATGGAGTCAGATTTTCAGTGAAGACACATTAAAGCAAACCATTCCTGTTGTAGCAGATTGGCGATTGAATGAAAGAATGTATGGGGAATTACAGGGTCTTAATAAGCAGGAAACAGCAGACAGATATGGGAAGGAACAAGTTCATGAGTGGCGTCGGAGTTATGACATTCCTCCTCCCAATGGCGAGAGTTTGGAAATGTGCGCTCAAAGAGCTGTTGCTTATTTCAAAGATCAAATTGAACCCCAACTGTTAGCTGGGAAGAATGTTATGATTGCTGCCCACGGGAATTCGCTGAGATCCATAATCATGTATCTTGACAAACTGACTTCTCAAGAGGTTATTAGTTTGGAACTATCAACTGGAATTCCTATGCTTTACATTTCCAAAGAGGGAAAGTTCATTCGGAGGGGAAGTCCTGCGGGACCTGCTGAGGCTGGGGTTTATGCCTACACTAAGAATTTGGCTCTTTACAGGCAGAAGTTAGATGAGATGGTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00260 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko05230 map00010 map00260 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map05230
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

352

Amino Acids

39.02

Weight (kDa)

9.15

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 101 - 172 7.5e-18 Histidine phosphatase superfamily (branch 1)
His_Phos_1 PF00300 171 - 304 4.4e-16 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 988
AccI GTMKAC 1 cut(s) 435
AciI CCGC 1 cut(s) 974
AclI AACGTT 1 cut(s) 192
AclWI GGATC 1 cut(s) 846
AcsI RAATTY 3 cut(s) 841, 918, 1012
AcuI CTGAAG 1 cut(s) 210
AcyI GRCGYC 1 cut(s) 706
AfiI CCNNNNNNNGG 1 cut(s) 734
AgsI TTSAA 6 cut(s) 95, 129, 404, 622, 781, 794
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 7 cut(s) 100, 111, 215, 299, 407, 767, 809
AluI AGCT 7 cut(s) 100, 111, 215, 299, 407, 767, 809
Alw21I GWGCWC 1 cut(s) 469
Alw44I GTGCAC 1 cut(s) 465
AlwI GGATC 1 cut(s) 846
AoxI GGCC 1 cut(s) 85
ApaLI GTGCAC 1 cut(s) 465
ApeKI GCWGC 7 cut(s) 17, 20, 23, 26, 29, 296, 830
ApoI RAATTY 3 cut(s) 841, 918, 1012
Asp700I GAANNNNTTC 2 cut(s) 347, 693
AspLEI GCGC 1 cut(s) 758
AspS9I GGNCC 2 cut(s) 514, 977
AsuHPI GGTGA 2 cut(s) 29, 332
AvaII GGWCC 2 cut(s) 514, 977
BaeGI GKGCMC 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 469
BbvCI CCTCAGC 2 cut(s) 211, 984
BbvI GCAGC 7 cut(s) 4, 7, 10, 13, 16, 308, 817
BccI CCATC 1 cut(s) 1039
BciT130I CCWGG 1 cut(s) 221
BfaI CTAG 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 988
BisI GCNGC 7 cut(s) 18, 21, 24, 27, 30, 297, 831
BlsI GCNGC 7 cut(s) 19, 22, 25, 28, 31, 298, 832
Bme1390I CCNGG 1 cut(s) 221
Bme18I GGWCC 2 cut(s) 514, 977
BmgT120I GGNCC 2 cut(s) 514, 977
BmiI GGNNCC 2 cut(s) 516, 978
BmrFI CCNGG 1 cut(s) 221
BmrI ACTGGG 1 cut(s) 512
BmsI GCATC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 582
Bpu10I CCTNAGC 2 cut(s) 211, 984
BpuEI CTTGAG 1 cut(s) 870
BsaHI GRCGYC 1 cut(s) 706
BsaJI CCNNGG 2 cut(s) 219, 835
BsaXI ACNNNNNCTCC 2 cut(s) 952, 982
Bsc4I CCNNNNNNNGG 1 cut(s) 734
Bse1I ACTGG 2 cut(s) 518, 919
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 219, 835
BseGI GGATG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 734
BseMII CTCAG 5 cut(s) 126, 225, 365, 839, 975
BseNI ACTGG 2 cut(s) 518, 919
BseRI GAGGAG 2 cut(s) 45, 717
BseSI GKGCMC 1 cut(s) 469
BseXI GCAGC 7 cut(s) 4, 7, 10, 13, 16, 308, 817
BseYI CCCAGC 2 cut(s) 809, 989
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 4 cut(s) 64, 353, 500, 990
BslI CCNNNNNNNGG 1 cut(s) 734
BsmFI GGGAC 4 cut(s) 64, 353, 500, 990
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 469
Bsp143I GATC 3 cut(s) 441, 784, 851
BspACI CCGC 1 cut(s) 974
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 5 cut(s) 125, 224, 366, 840, 976
BspHI TCATGA 1 cut(s) 697
BspLI GGNNCC 2 cut(s) 516, 978
BspMI ACCTGC 1 cut(s) 988
BspPI GGATC 1 cut(s) 846
BsrI ACTGG 2 cut(s) 518, 919
BssECI CCNNGG 2 cut(s) 219, 835
BssMI GATC 3 cut(s) 441, 784, 851
BssNI GRCGYC 1 cut(s) 706
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 4 cut(s) 156, 330, 503, 804
Bst6I CTCTTC 2 cut(s) 184, 387
BstACI GRCGYC 1 cut(s) 706
BstC8I GCNNGC 4 cut(s) 89, 102, 409, 486
BstDEI CTNAG 6 cut(s) 112, 211, 374, 848, 984, 1008
BstDSI CCRYGG 1 cut(s) 835
BstF5I GGATG 1 cut(s) 173
BstHHI GCGC 1 cut(s) 758
BstKTI GATC 3 cut(s) 444, 787, 854
BstMBI GATC 3 cut(s) 441, 784, 851
BstMWI GCNNNNNNNGC 9 cut(s) 23, 26, 29, 97, 221, 404, 485, 764, 980
BstNI CCWGG 1 cut(s) 221
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 219
BstSLI GKGCMC 1 cut(s) 469
BstV1I GCAGC 7 cut(s) 4, 7, 10, 13, 16, 308, 817
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 1 cut(s) 851
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 1 cut(s) 851
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 835
BtsCI GGATG 1 cut(s) 173
BtsIMutI CAGTG 5 cut(s) 120, 234, 326, 455, 577
BveI ACCTGC 1 cut(s) 988
Cac8I GCNNGC 4 cut(s) 89, 102, 409, 486
CciI TCATGA 1 cut(s) 697
CfoI GCGC 1 cut(s) 758
Cfr13I GGNCC 2 cut(s) 514, 977
CseI GACGC 1 cut(s) 695
CviAII CATG 8 cut(s) 71, 317, 439, 481, 490, 555, 698, 862
DdeI CTNAG 6 cut(s) 112, 211, 374, 848, 984, 1008
DpnI GATC 3 cut(s) 443, 786, 853
DpnII GATC 3 cut(s) 441, 784, 851
Eam1104I CTCTTC 2 cut(s) 184, 387
EarI CTCTTC 2 cut(s) 184, 387
Eco47I GGWCC 2 cut(s) 514, 977
Eco57I CTGAAG 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 514, 977
EcoRI GAATTC 2 cut(s) 841, 918
EcoRII CCWGG 1 cut(s) 219
EcoT22I ATGCAT 1 cut(s) 531
FaeI CATG 8 cut(s) 74, 320, 442, 484, 493, 558, 701, 865
FaqI GGGAC 4 cut(s) 64, 353, 500, 990
FatI CATG 8 cut(s) 70, 316, 438, 480, 489, 554, 697, 861
FauI CCCGC 1 cut(s) 967
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 7 cut(s) 18, 21, 24, 27, 30, 297, 831
FokI GGATG 1 cut(s) 180
Fsp4HI GCNGC 7 cut(s) 18, 21, 24, 27, 30, 297, 831
FspBI CTAG 1 cut(s) 549
GlaI GCGC 1 cut(s) 757
GluI GCNGC 7 cut(s) 18, 21, 24, 27, 30, 297, 831
GsaI CCCAGC 2 cut(s) 813, 993
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 84
HgaI GACGC 1 cut(s) 695
HhaI GCGC 1 cut(s) 758
Hin1I GRCGYC 1 cut(s) 706
Hin1II CATG 8 cut(s) 74, 320, 442, 484, 493, 558, 701, 865
Hin6I GCGC 1 cut(s) 756
HinP1I GCGC 1 cut(s) 756
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 6 cut(s) 52, 272, 323, 417, 460, 559
HpaII CCGG 1 cut(s) 84
HphI GGTGA 2 cut(s) 29, 332
Hpy166II GTNNAC 4 cut(s) 351, 436, 467, 542
Hpy188I TCNGA 6 cut(s) 279, 375, 507, 564, 711, 960
Hpy188III TCNNGA 4 cut(s) 95, 698, 869, 887
Hpy8I GTNNAC 4 cut(s) 351, 436, 467, 542
Hpy99I CGWCG 2 cut(s) 507, 711
HpyAV CCTTC 3 cut(s) 376, 505, 679
HpyCH4III ACNGT 4 cut(s) 156, 330, 503, 804
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 4 cut(s) 32, 455, 467, 529
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 26, 29, 97, 221, 404, 485, 764, 980
HpyF3I CTNAG 6 cut(s) 112, 211, 374, 848, 984, 1008
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 706
Hsp92II CATG 8 cut(s) 74, 320, 442, 484, 493, 558, 701, 865
HspAI GCGC 1 cut(s) 756
Kzo9I GATC 3 cut(s) 441, 784, 851
Lsp1109I GCAGC 7 cut(s) 4, 7, 10, 13, 16, 308, 817
LweI GCATC 1 cut(s) 158
MaeI CTAG 1 cut(s) 549
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 324
MalI GATC 3 cut(s) 443, 786, 853
MboI GATC 3 cut(s) 441, 784, 851
MboII GAAGA 5 cut(s) 171, 404, 520, 587, 826
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 1 cut(s) 851
MhlI GDGCHC 1 cut(s) 469
MluCI AATT 8 cut(s) 79, 201, 399, 641, 789, 841, 918, 1012
MlyI GAGTC 3 cut(s) 46, 332, 568
MmeI TCCRAC 2 cut(s) 485, 689
Mph1103I ATGCAT 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 347, 693
MseI TTAA 3 cut(s) 75, 584, 654
MslI CAYNNNNRTG 1 cut(s) 860
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 221
MunI CAATTG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 221
MwoI GCNNNNNNNGC 9 cut(s) 23, 26, 29, 97, 221, 404, 485, 764, 980
NdeII GATC 3 cut(s) 441, 784, 851
NlaIII CATG 8 cut(s) 74, 320, 442, 484, 493, 558, 701, 865
NlaIV GGNNCC 2 cut(s) 516, 978
NmuCI GTSAC 1 cut(s) 324
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 74
PagI TCATGA 1 cut(s) 697
PdmI GAANNNNTTC 2 cut(s) 347, 693
PfeI GAWTC 3 cut(s) 272, 417, 460
PkrI GCNGC 7 cut(s) 19, 22, 25, 28, 31, 298, 832
PleI GAGTC 3 cut(s) 46, 331, 567
PpsI GAGTC 3 cut(s) 46, 331, 567
PpuMI RGGWCCY 2 cut(s) 514, 977
PsiI TTATAA 1 cut(s) 524
Psp1406I AACGTT 1 cut(s) 192
Psp5II RGGWCCY 2 cut(s) 514, 977
Psp6I CCWGG 1 cut(s) 219
PspFI CCCAGC 2 cut(s) 809, 989
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 2 cut(s) 516, 978
PspPI GGNCC 2 cut(s) 514, 977
PspPPI RGGWCCY 2 cut(s) 514, 977
PsuI RGATCY 1 cut(s) 851
RseI CAYNNNNRTG 1 cut(s) 860
SalI GTCGAC 1 cut(s) 434
SaqAI TTAA 3 cut(s) 75, 584, 654
SatI GCNGC 7 cut(s) 18, 21, 24, 27, 30, 297, 831
Sau3AI GATC 3 cut(s) 441, 784, 851
Sau96I GGNCC 2 cut(s) 514, 977
SchI GAGTC 3 cut(s) 46, 332, 568
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 1 cut(s) 469
SfaNI GCATC 1 cut(s) 158
SinI GGWCC 2 cut(s) 514, 977
SmiMI CAYNNNNRTG 1 cut(s) 860
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
Sse9I AATT 8 cut(s) 79, 201, 399, 641, 789, 841, 918, 1012
SsiI CCGC 1 cut(s) 974
SspMI CTAG 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 156, 330, 503, 804
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 205, 435
TasI AATT 8 cut(s) 79, 201, 399, 641, 789, 841, 918, 1012
TfiI GAWTC 3 cut(s) 272, 417, 460
Tru1I TTAA 3 cut(s) 75, 584, 654
Tru9I TTAA 3 cut(s) 75, 584, 654
TscAI CASTG 5 cut(s) 120, 234, 333, 462, 577
TseFI GTSAC 1 cut(s) 324
TseI GCWGC 7 cut(s) 17, 20, 23, 26, 29, 296, 830
Tsp45I GTSAC 1 cut(s) 324
TspDTI ATGAA 7 cut(s) 112, 234, 306, 639, 686, 943, 1040
TspRI CASTG 5 cut(s) 120, 234, 333, 462, 577
VneI GTGCAC 1 cut(s) 465
VpaK11BI GGWCC 2 cut(s) 514, 977
XapI RAATTY 3 cut(s) 841, 918, 1012
XceI RCATGY 1 cut(s) 74
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 435
XmnI GAANNNNTTC 2 cut(s) 347, 693
XspI CTAG 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.