Rroxscaffold_3G00220740

Phosphoglycerate mutase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001245
Physical Location & Seq
Forward (+)
3121384 .. 3125083
3700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00220740.1

Sequence Viewer

Length: 1041 bp
ATGGCTACTGCTGTGTTTCACCAATCCATTGGAACTCACCAGTCCCATGGACATCTCTATAACTCTGGCATTCATCAAGAGTTGGGGAAAAGTTCAGTGAGATTGATTTCAAAGGCGTTCGAGGTTGATGTTGGACTGTCAAGAAGAGTATGCTACAACTCTGGTCAGAGAAACCTTGGTGTAATTCAAGCTTCGACTTCCCAAACTTCAGTGATCGATCATGTTTCGTCCCCCTCAAAGAACAAGGAAAGTGACTCGCGAAAGAAATCTAATGAAGCAGCTTTGATTTTGATTAGGCATGGTGAATCATTGTGGAACGAAAAGAACTTGTTCACTGGCTGTGTTGATGTGCCGCTGAGCAAGAAGGGTGTAGAAGAGGCACTTGAAGCTGGCAAGAGAATCAGCAACATACCTGTCGACATGATCTATACATCTGCACTGATTCGTGCACAGATGACTGCCATGCTTGCTATGACACAGCACCGTCGTAAGAAGGTGCCAATTATGTTGCATAATGAAAGCGAACAGGCTAGGGCATGGAGCCAGATTTTTAGTGAAGACACAAAATTGCAATCCATTCCTGTGGTCGCGGATTGGCGCTTGAATGAAAGAATGTTGTATGGAGAATTACAGGGTCTCAATAAACAAGAAACAGCAGATAGATATGGGAAGGAACAGGTTCACGAGTGGCGTCGGAGTTATGATGTACCCCCTCCCAATGGCGAGAGTTTGGAAATGTGTGCTGAAAGAGCTGTTGCTTATTTTAAAGATCAAATTGAACCCCAACTCTTATCTGGAAAGAATATTATGATTGCTGCCCACGGGAATTCATTGAGATCTATTATCATGTATCTGGATAAGCTAACTTCCCAAGAGGTTATTAGTTTAGAACTGTCAACTGGAATACCGATGCTTTACATTTTCAAAGAGGGAAAATTCACTCGGAGGGGAAGTCCTTCGGCACCTACTGAGGCTGGGGTTTATGCCTACACCAAGAAGTTGGCCCTTTACAGGCAGAAGTTAGATGAGATGGTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00260 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko05230 map00010 map00260 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map05230
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

346

Amino Acids

38.93

Weight (kDa)

9.04

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 95 - 162 5.8e-18 Histidine phosphatase superfamily (branch 1)
His_Phos_1 PF00300 182 - 300 1.4e-13 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 496, 961
AccI GTMKAC 1 cut(s) 417
AccII CGCG 2 cut(s) 259, 590
AciI CCGC 2 cut(s) 353, 590
AcsI RAATTY 2 cut(s) 826, 935
AcuI CTGAAG 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 691
AfaI GTAC 1 cut(s) 708
AfiI CCNNNNNNNGG 2 cut(s) 719, 1011
AgsI TTSAA 6 cut(s) 111, 188, 386, 604, 779, 925
AluBI AGCT 5 cut(s) 191, 281, 389, 752, 862
AluI AGCT 5 cut(s) 191, 281, 389, 752, 862
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 641
Alw44I GTGCAC 1 cut(s) 447
AoxI GGCC 1 cut(s) 1002
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 2 cut(s) 278, 815
ApoI RAATTY 2 cut(s) 826, 935
Asp700I GAANNNNTTC 3 cut(s) 329, 678, 955
AspLEI GCGC 1 cut(s) 600
AspS9I GGNCC 1 cut(s) 1003
AsuHPI GGTGA 3 cut(s) 11, 29, 314
BaeGI GKGCMC 1 cut(s) 451
BanI GGYRCC 2 cut(s) 496, 961
BauI CACGAG 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 2 cut(s) 290, 802
BccI CCATC 1 cut(s) 1024
BcoDI GTCTC 1 cut(s) 641
BfaI CTAG 1 cut(s) 531
BfoI RGCGCY 1 cut(s) 601
BglII AGATCT 1 cut(s) 836
BisI GCNGC 3 cut(s) 279, 353, 816
BlpI GCTNAGC 1 cut(s) 356
BlsI GCNGC 3 cut(s) 280, 354, 817
BmgT120I GGNCC 1 cut(s) 1003
BmiI GGNNCC 3 cut(s) 498, 542, 963
BmsI GCATC 1 cut(s) 900
BpiI GAAGAC 1 cut(s) 564
Bpu1102I GCTNAGC 1 cut(s) 356
Bsa29I ATCGAT 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 691
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 3 cut(s) 46, 175, 820
BsaXI ACNNNNNCTCC 2 cut(s) 937, 967
Bsc4I CCNNNNNNNGG 2 cut(s) 719, 1011
Bse1I ACTGG 3 cut(s) 40, 340, 904
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 3 cut(s) 46, 175, 820
BseLI CCNNNNNNNGG 2 cut(s) 719, 1011
BseMII CTCAG 2 cut(s) 347, 960
BseNI ACTGG 3 cut(s) 40, 340, 904
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 2 cut(s) 290, 802
BseYI CCCAGC 1 cut(s) 974
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 259, 590
BshFI GGCC 1 cut(s) 1004
BshNI GGYRCC 2 cut(s) 496, 961
BshVI ATCGAT 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 451
BslFI GGGAC 2 cut(s) 28, 214
BslI CCNNNNNNNGG 2 cut(s) 719, 1011
BsmAI GTCTC 1 cut(s) 641
BsmFI GGGAC 2 cut(s) 28, 214
BsmI GAATGC 1 cut(s) 69
BsnI GGCC 1 cut(s) 1004
Bso31I GGTCTC 1 cut(s) 641
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 5 cut(s) 213, 217, 423, 769, 836
Bsp1720I GCTNAGC 1 cut(s) 356
Bsp19I CCATGG 1 cut(s) 46
Bsp68I TCGCGA 1 cut(s) 259
BspACI CCGC 2 cut(s) 353, 590
BspANI GGCC 1 cut(s) 1004
BspCNI CTCAG 2 cut(s) 348, 961
BspDI ATCGAT 1 cut(s) 216
BspFNI CGCG 2 cut(s) 259, 590
BspLI GGNNCC 3 cut(s) 498, 542, 963
BspT107I GGYRCC 2 cut(s) 496, 961
BspTNI GGTCTC 1 cut(s) 641
BsrI ACTGG 3 cut(s) 40, 340, 904
BssECI CCNNGG 3 cut(s) 46, 175, 820
BssMI GATC 5 cut(s) 213, 217, 423, 769, 836
BssNI GRCGYC 1 cut(s) 691
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 2 cut(s) 46, 175
Bst2BI CACGAG 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 138, 485, 894
Bst6I CTCTTC 2 cut(s) 139, 369
BstACI GRCGYC 1 cut(s) 691
BstC8I GCNNGC 2 cut(s) 391, 468
BstDEI CTNAG 2 cut(s) 356, 969
BstDSI CCRYGG 2 cut(s) 46, 820
BstFNI CGCG 2 cut(s) 259, 590
BstH2I RGCGCY 1 cut(s) 601
BstHHI GCGC 1 cut(s) 600
BstKTI GATC 5 cut(s) 216, 220, 426, 772, 839
BstMAI GTCTC 1 cut(s) 641
BstMBI GATC 5 cut(s) 213, 217, 423, 769, 836
BstMWI GCNNNNNNNGC 3 cut(s) 386, 467, 749
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 2 cut(s) 259, 590
BstV1I GCAGC 2 cut(s) 290, 802
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 1 cut(s) 836
BstXI CCANNNNNNTGG 4 cut(s) 29, 47, 583, 1000
BstYI RGATCY 1 cut(s) 836
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 1 cut(s) 1004
BsuTUI ATCGAT 1 cut(s) 216
BtgI CCRYGG 2 cut(s) 46, 820
BtsIMutI CAGTG 4 cut(s) 102, 216, 333, 437
BtuMI TCGCGA 1 cut(s) 259
Cac8I GCNNGC 2 cut(s) 391, 468
CfoI GCGC 1 cut(s) 600
Cfr13I GGNCC 1 cut(s) 1003
ClaI ATCGAT 1 cut(s) 216
CseI GACGC 1 cut(s) 680
Csp6I GTAC 1 cut(s) 707
CviAII CATG 7 cut(s) 47, 221, 299, 421, 463, 537, 847
CviQI GTAC 1 cut(s) 707
DdeI CTNAG 2 cut(s) 356, 969
DpnI GATC 5 cut(s) 215, 219, 425, 771, 838
DpnII GATC 5 cut(s) 213, 217, 423, 769, 836
DraI TTTAAA 1 cut(s) 766
Eam1104I CTCTTC 2 cut(s) 139, 369
EarI CTCTTC 2 cut(s) 139, 369
Eco130I CCWWGG 2 cut(s) 46, 175
Eco31I GGTCTC 1 cut(s) 641
Eco57I CTGAAG 1 cut(s) 192
EcoRI GAATTC 1 cut(s) 826
EcoT14I CCWWGG 2 cut(s) 46, 175
ErhI CCWWGG 2 cut(s) 46, 175
FaeI CATG 7 cut(s) 50, 224, 302, 424, 466, 540, 850
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 2 cut(s) 28, 214
FatI CATG 7 cut(s) 46, 220, 298, 420, 462, 536, 846
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 279, 353, 816
Fsp4HI GCNGC 3 cut(s) 279, 353, 816
FspBI CTAG 1 cut(s) 531
GlaI GCGC 1 cut(s) 599
GluI GCNGC 3 cut(s) 279, 353, 816
GsaI CCCAGC 1 cut(s) 978
HaeII RGCGCY 1 cut(s) 601
HaeIII GGCC 1 cut(s) 1004
HgaI GACGC 1 cut(s) 680
HhaI GCGC 1 cut(s) 600
Hin1I GRCGYC 1 cut(s) 691
Hin1II CATG 7 cut(s) 50, 224, 302, 424, 466, 540, 850
Hin6I GCGC 1 cut(s) 598
HinP1I GCGC 1 cut(s) 598
HincII GTYRAC 2 cut(s) 418, 897
HindII GTYRAC 2 cut(s) 418, 897
HindIII AAGCTT 1 cut(s) 189
HinfI GANTC 4 cut(s) 254, 305, 399, 442
HphI GGTGA 3 cut(s) 11, 29, 314
Hpy166II GTNNAC 5 cut(s) 333, 418, 449, 682, 897
Hpy188I TCNGA 3 cut(s) 168, 696, 945
Hpy188III TCNNGA 6 cut(s) 77, 141, 258, 683, 795, 854
Hpy8I GTNNAC 5 cut(s) 333, 418, 449, 682, 897
Hpy99I CGWCG 2 cut(s) 489, 696
HpyAV CCTTC 4 cut(s) 358, 487, 664, 966
HpyCH4III ACNGT 3 cut(s) 138, 485, 894
HpyCH4V TGCA 4 cut(s) 437, 449, 511, 571
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 467, 749
HpyF3I CTNAG 2 cut(s) 356, 969
Hsp92I GRCGYC 1 cut(s) 691
Hsp92II CATG 7 cut(s) 50, 224, 302, 424, 466, 540, 850
HspAI GCGC 1 cut(s) 598
Kzo9I GATC 5 cut(s) 213, 217, 423, 769, 836
LmnI GCTCC 1 cut(s) 540
Lsp1109I GCAGC 2 cut(s) 290, 802
LweI GCATC 1 cut(s) 900
MaeI CTAG 1 cut(s) 531
MaeIII GTNAC 1 cut(s) 251
MalI GATC 5 cut(s) 215, 219, 425, 771, 838
MboI GATC 5 cut(s) 213, 217, 423, 769, 836
MboII GAAGA 3 cut(s) 156, 386, 569
MflI RGATCY 1 cut(s) 836
MhlI GDGCHC 1 cut(s) 451
MluCI AATT 7 cut(s) 183, 501, 566, 626, 774, 826, 935
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 2 cut(s) 112, 674
MnlI CCTC 8 cut(s) 115, 244, 370, 723, 868, 922, 939, 964
MroXI GAANNNNTTC 3 cut(s) 329, 678, 955
MseI TTAA 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 355
Mva1269I GAATGC 1 cut(s) 69
MvnI CGCG 2 cut(s) 259, 590
MwoI GCNNNNNNNGC 3 cut(s) 386, 467, 749
NcoI CCATGG 1 cut(s) 46
NdeII GATC 5 cut(s) 213, 217, 423, 769, 836
NlaIII CATG 7 cut(s) 50, 224, 302, 424, 466, 540, 850
NlaIV GGNNCC 3 cut(s) 498, 542, 963
NmuCI GTSAC 1 cut(s) 251
NruI TCGCGA 1 cut(s) 259
PctI GAATGC 1 cut(s) 69
PdmI GAANNNNTTC 3 cut(s) 329, 678, 955
PfeI GAWTC 3 cut(s) 305, 399, 442
PkrI GCNGC 3 cut(s) 280, 354, 817
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 974
PspN4I GGNNCC 3 cut(s) 498, 542, 963
PspPI GGNCC 1 cut(s) 1003
PsuI RGATCY 1 cut(s) 836
RruI TCGCGA 1 cut(s) 259
RsaI GTAC 1 cut(s) 708
RsaNI GTAC 1 cut(s) 707
RseI CAYNNNNRTG 1 cut(s) 581
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 1 cut(s) 765
SatI GCNGC 3 cut(s) 279, 353, 816
Sau3AI GATC 5 cut(s) 213, 217, 423, 769, 836
Sau96I GGNCC 1 cut(s) 1003
SchI GAGTC 1 cut(s) 248
SduI GDGCHC 1 cut(s) 451
SfaNI GCATC 1 cut(s) 900
SmiMI CAYNNNNRTG 1 cut(s) 581
Sse9I AATT 7 cut(s) 183, 501, 566, 626, 774, 826, 935
SsiI CCGC 2 cut(s) 353, 590
SspI AATATT 1 cut(s) 805
SspMI CTAG 1 cut(s) 531
StyI CCWWGG 2 cut(s) 46, 175
TaaI ACNGT 3 cut(s) 138, 485, 894
TaqI TCGA 4 cut(s) 120, 194, 216, 417
TasI AATT 7 cut(s) 183, 501, 566, 626, 774, 826, 935
TauI GCSGC 1 cut(s) 355
TfiI GAWTC 3 cut(s) 305, 399, 442
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 4 cut(s) 102, 216, 340, 444
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 2 cut(s) 278, 815
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 6 cut(s) 62, 288, 531, 621, 819, 1025
TspRI CASTG 4 cut(s) 102, 216, 340, 444
VneI GTGCAC 1 cut(s) 447
XapI RAATTY 2 cut(s) 826, 935
XcmI CCANNNNNNNNNTGG 1 cut(s) 791
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 3 cut(s) 329, 678, 955
XspI CTAG 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.