pycom15g32710

Phosphoglycerate mutase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001245
Physical Location & Seq
Forward (+)
31915983 .. 31917688
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32710.2

Sequence Viewer

Length: 585 bp
ATGACTGCCATGCGTGCCATGACACAGCACCGTCGTAAGAAGGTCCCAGTTATAATGCATAATGAGAGTGAACGGGCTAGGGCATGGAGTCAGATTTTCAGTGAAGACACAATAAAGCAAACCATTCCTGTCGTAGCAGATTGGCGATTGAATGAAAGAATGTATGGGGAATTACAGGGTCTTAATAAGCAGGAAACAGCAGACAGATATGGGAAGGAACAAGTTCACGAGTGGCGTCGGAGTTATGACATTCCCCCTCCCAATGGCGAGAGTTTGGAAATGTGCGCTCAAAGAGCTGTTGCTTATTTCAAAGATCAAATTGAACCCCAACTGTTGGCTGGGAAGAATGTTATGATTGCTGCGCACGGGAATTCGCTGAGATCCATAATCATGTATCTTGACAAACTGACTTCTCAAGAGGTTATTAGTTTGGAACTATCAACTGGAATTCCTATGCTTTACATTTCCAAAGAGGGAAAATTCATCCGGAGGGGAAGTCCTGCGGGACCTGCTGAGGCTGGGGTTTATGCCTACACTAAGAATTTGGCTCTTTACAGGCAGAAGTTAGATGAGATGGTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00260 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko05230 map00010 map00260 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map05230
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

195

Amino Acids

22.27

Weight (kDa)

9.12

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 53
Acc16I TGCGCA 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 517
AccB7I CCANNNNNTGG 1 cut(s) 334
AccIII TCCGGA 1 cut(s) 486
AciI CCGC 1 cut(s) 503
AclWI GGATC 1 cut(s) 375
AcsI RAATTY 4 cut(s) 370, 447, 479, 541
AcyI GRCGYC 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 263, 334
AgsI TTSAA 3 cut(s) 151, 310, 323
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
AlwI GGATC 1 cut(s) 375
Aor13HI TCCGGA 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 4 cut(s) 370, 447, 479, 541
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 2 cut(s) 287, 364
AspS9I GGNCC 2 cut(s) 43, 506
AvaII GGWCC 2 cut(s) 43, 506
BauI CACGAG 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 111
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 346
BccI CCATC 1 cut(s) 568
BfaI CTAG 1 cut(s) 78
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme18I GGWCC 2 cut(s) 43, 506
BmgT120I GGNCC 2 cut(s) 43, 506
BmiI GGNNCC 2 cut(s) 45, 507
BmrI ACTGGG 1 cut(s) 41
BmuI ACTGGG 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 111
Bpu10I CCTNAGC 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 486
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 334
Bse1I ACTGG 2 cut(s) 47, 448
BseAI TCCGGA 1 cut(s) 486
BseGI GGATG 1 cut(s) 483
BseLI CCNNNNNNNGG 2 cut(s) 263, 334
BseMII CTCAG 2 cut(s) 368, 504
BseNI ACTGG 2 cut(s) 47, 448
BseXI GCAGC 1 cut(s) 346
BseYI CCCAGC 2 cut(s) 338, 518
BsiSI CCGG 1 cut(s) 487
BslFI GGGAC 2 cut(s) 29, 519
BslI CCNNNNNNNGG 2 cut(s) 263, 334
BsmFI GGGAC 2 cut(s) 29, 519
Bsp13I TCCGGA 1 cut(s) 486
Bsp143I GATC 2 cut(s) 313, 380
BspACI CCGC 1 cut(s) 503
BspCNI CTCAG 2 cut(s) 369, 505
BspEI TCCGGA 1 cut(s) 486
BspLI GGNNCC 2 cut(s) 45, 507
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 1 cut(s) 375
BsrI ACTGG 2 cut(s) 47, 448
BssMI GATC 2 cut(s) 313, 380
BssNI GRCGYC 1 cut(s) 235
BssSI CACGAG 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 227
Bst4CI ACNGT 2 cut(s) 32, 333
BstACI GRCGYC 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 3 cut(s) 377, 513, 537
BstF5I GGATG 1 cut(s) 483
BstHHI GCGC 2 cut(s) 287, 364
BstKTI GATC 2 cut(s) 316, 383
BstMBI GATC 2 cut(s) 313, 380
BstMWI GCNNNNNNNGC 3 cut(s) 14, 293, 509
BstV1I GCAGC 1 cut(s) 346
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
BtsCI GGATG 1 cut(s) 483
BtsIMutI CAGTG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 287, 364
Cfr13I GGNCC 2 cut(s) 43, 506
CseI GACGC 1 cut(s) 224
CviAII CATG 4 cut(s) 10, 19, 84, 391
CviJI RGCY 5 cut(s) 77, 296, 338, 518, 548
CviKI_1 RGCY 5 cut(s) 77, 296, 338, 518, 548
DdeI CTNAG 3 cut(s) 377, 513, 537
DpnI GATC 2 cut(s) 315, 382
DpnII GATC 2 cut(s) 313, 380
Eco47I GGWCC 2 cut(s) 43, 506
EcoO109I RGGNCCY 2 cut(s) 43, 506
EcoRI GAATTC 2 cut(s) 370, 447
EcoT22I ATGCAT 1 cut(s) 60
FaeI CATG 4 cut(s) 13, 22, 87, 394
FaqI GGGAC 2 cut(s) 29, 519
FatI CATG 4 cut(s) 9, 18, 83, 390
FauI CCCGC 1 cut(s) 496
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 1 cut(s) 78
FspI TGCGCA 1 cut(s) 363
GlaI GCGC 2 cut(s) 286, 363
GluI GCNGC 1 cut(s) 360
GsaI CCCAGC 2 cut(s) 342, 522
HapII CCGG 1 cut(s) 487
HgaI GACGC 1 cut(s) 224
HhaI GCGC 2 cut(s) 287, 364
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 4 cut(s) 13, 22, 87, 394
Hin6I GCGC 2 cut(s) 285, 362
HinP1I GCGC 2 cut(s) 285, 362
HinfI GANTC 1 cut(s) 88
HpaII CCGG 1 cut(s) 487
Hpy166II GTNNAC 2 cut(s) 71, 226
Hpy188I TCNGA 2 cut(s) 93, 240
Hpy188III TCNNGA 4 cut(s) 227, 398, 416, 487
Hpy8I GTNNAC 2 cut(s) 71, 226
Hpy99I CGWCG 2 cut(s) 36, 240
HpyAV CCTTC 2 cut(s) 34, 208
HpyCH4III ACNGT 2 cut(s) 32, 333
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 293, 509
HpyF3I CTNAG 3 cut(s) 377, 513, 537
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 4 cut(s) 13, 22, 87, 394
HspAI GCGC 2 cut(s) 285, 362
Kpn2I TCCGGA 1 cut(s) 486
Kzo9I GATC 2 cut(s) 313, 380
Lsp1109I GCAGC 1 cut(s) 346
MaeI CTAG 1 cut(s) 78
MalI GATC 2 cut(s) 315, 382
MboI GATC 2 cut(s) 313, 380
MboII GAAGA 2 cut(s) 116, 355
MflI RGATCY 1 cut(s) 380
MluCI AATT 6 cut(s) 170, 318, 370, 447, 479, 541
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 5 cut(s) 267, 412, 466, 483, 508
Mph1103I ATGCAT 1 cut(s) 60
MroI TCCGGA 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 1 cut(s) 487
MwoI GCNNNNNNNGC 3 cut(s) 14, 293, 509
NdeII GATC 2 cut(s) 313, 380
NlaIII CATG 4 cut(s) 13, 22, 87, 394
NlaIV GGNNCC 2 cut(s) 45, 507
NsbI TGCGCA 1 cut(s) 363
NsiI ATGCAT 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 222
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
PpuMI RGGWCCY 2 cut(s) 43, 506
PsiI TTATAA 1 cut(s) 53
Psp5II RGGWCCY 2 cut(s) 43, 506
PspFI CCCAGC 2 cut(s) 338, 518
PspN4I GGNNCC 2 cut(s) 45, 507
PspPI GGNCC 2 cut(s) 43, 506
PspPPI RGGWCCY 2 cut(s) 43, 506
PsuI RGATCY 1 cut(s) 380
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 2 cut(s) 313, 380
Sau96I GGNCC 2 cut(s) 43, 506
SchI GAGTC 1 cut(s) 97
SetI ASST 4 cut(s) 45, 298, 423, 511
SinI GGWCC 2 cut(s) 43, 506
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 414
SmoI CTYRAG 1 cut(s) 414
Sse9I AATT 6 cut(s) 170, 318, 370, 447, 479, 541
SsiI CCGC 1 cut(s) 503
SspMI CTAG 1 cut(s) 78
TaaI ACNGT 2 cut(s) 32, 333
TasI AATT 6 cut(s) 170, 318, 370, 447, 479, 541
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 1 cut(s) 106
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 3 cut(s) 168, 472, 569
TspRI CASTG 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 43, 506
XapI RAATTY 4 cut(s) 370, 447, 479, 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.