pycom08g15360

Phosphoglycerate mutase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001245
Physical Location & Seq
Forward (+)
14999196 .. 15001216
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15360.3

Sequence Viewer

Length: 834 bp
ATGGTTTCATCCCCCTCGCATACCAAAGCCAATGATTCTCGAAAGAAATCTAATGAAGCAGCTTTGATTTTGATTAGGCATGGCGAGTCATTGTGGAACGAAAAGAACTTGTTCACAGGCTGTGTTGATGTCCCTCTGAGCAAGAAGGGTGTGGAAGAGGCAATTGAAGCTGGCAAGAGAATCAGCAACATACCTGTCGACATGATCTATACATCTGCACTGATTCGTGCACAGATGACTGCCATGCTTGCCATGACACAGCACCGTCGTAAGAAGGTCCCAGTTATAATGCATAACGAGAGTGAACGGGCTAGGGCATGGGGTCAGATTTTCAGTGAAGACACAATAAAGCAAACCATTCCTGTCGTTGCAGATTGGCGATTGAATGAAAGAATGTATGGGCAATTACAGGGTCTTAATAAGCAGGAAACAGCAGATAGATATGGGAAGGAACAAGTTCACGAGTGGCGTCGGAGTTATGACATTCCCCCTCCCAATGGGGAGAGTTTGGAAATGTGTGCTCAAAGAGCTGTTGCTTATTTCAAAGATCAAATTGAACCCCAACTGTTATCTGGAAAGAATGTTATGATTGCTGCCCACGGGAATTCGTTGAGATCCATAATCATGTATCTTGACAAACTGACTTCTCAAGAGGTTATCAGTTTAGAACTATCAACTGGAATACCCATGCTTTACATTTCCAAAGAGGGAAAATTCATTCGGAGGGGAAGTCCTGCGGGGCCTGCTGAGGCTGGGGTTTATGCCTACACCAAGAGTTTGGCTCTTTACAGGCAGAAGTTAGATGAGATGGTTCATTGGTGTGCTCCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00260 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko05230 map00010 map00260 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map05230
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

278

Amino Acids

31.28

Weight (kDa)

9.11

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
AccI GTMKAC 1 cut(s) 198
AciI CCGC 1 cut(s) 737
AclWI GGATC 1 cut(s) 609
AcsI RAATTY 2 cut(s) 604, 713
AcyI GRCGYC 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 4 cut(s) 167, 385, 544, 557
AluBI AGCT 3 cut(s) 62, 170, 530
AluI AGCT 3 cut(s) 62, 170, 530
Alw21I GWGCWC 3 cut(s) 232, 523, 826
Alw44I GTGCAC 1 cut(s) 228
AlwI GGATC 1 cut(s) 609
AoxI GGCC 1 cut(s) 740
ApaLI GTGCAC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 59, 593
ApoI RAATTY 2 cut(s) 604, 713
Asp700I GAANNNNTTC 2 cut(s) 110, 456
AspS9I GGNCC 2 cut(s) 277, 740
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 232
BauI CACGAG 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 3 cut(s) 232, 523, 826
BbvCI CCTCAGC 1 cut(s) 747
BbvI GCAGC 2 cut(s) 71, 580
BccI CCATC 1 cut(s) 802
BfaI CTAG 2 cut(s) 312, 832
BisI GCNGC 2 cut(s) 60, 594
BlsI GCNGC 2 cut(s) 61, 595
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 2 cut(s) 277, 740
BmiI GGNNCC 2 cut(s) 279, 741
BmrI ACTGGG 1 cut(s) 275
BmuI ACTGGG 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 345
BplI GAGNNNNNCTC 2 cut(s) 766, 798
Bpu10I CCTNAGC 1 cut(s) 747
BpuEI CTTGAG 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 715, 745
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse1I ACTGG 2 cut(s) 281, 682
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 2 cut(s) 128, 738
BseNI ACTGG 2 cut(s) 281, 682
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 2 cut(s) 71, 580
BseYI CCCAGC 1 cut(s) 752
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 1 cut(s) 742
BsiHKAI GWGCWC 3 cut(s) 232, 523, 826
BslFI GGGAC 2 cut(s) 116, 263
BslI CCNNNNNNNGG 1 cut(s) 497
BsmFI GGGAC 2 cut(s) 116, 263
BsnI GGCC 1 cut(s) 742
Bsp1286I GDGCHC 3 cut(s) 232, 523, 826
Bsp143I GATC 3 cut(s) 204, 547, 614
BspACI CCGC 1 cut(s) 737
BspANI GGCC 1 cut(s) 742
BspCNI CTCAG 2 cut(s) 129, 739
BspLI GGNNCC 2 cut(s) 279, 741
BspPI GGATC 1 cut(s) 609
BsrI ACTGG 2 cut(s) 281, 682
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 3 cut(s) 204, 547, 614
BssNI GRCGYC 1 cut(s) 469
BssSI CACGAG 1 cut(s) 461
Bst2BI CACGAG 1 cut(s) 461
Bst4CI ACNGT 2 cut(s) 266, 567
Bst6I CTCTTC 1 cut(s) 150
BstACI GRCGYC 1 cut(s) 469
BstC8I GCNNGC 3 cut(s) 172, 249, 744
BstDEI CTNAG 2 cut(s) 137, 747
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 1 cut(s) 8
BstKTI GATC 3 cut(s) 207, 550, 617
BstMBI GATC 3 cut(s) 204, 547, 614
BstMWI GCNNNNNNNGC 4 cut(s) 167, 248, 527, 743
BstSLI GKGCMC 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 71, 580
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 614
BstXI CCANNNNNNTGG 1 cut(s) 778
BstYI RGATCY 1 cut(s) 614
BsuRI GGCC 1 cut(s) 742
BtgI CCRYGG 1 cut(s) 598
BtsCI GGATG 1 cut(s) 8
BtsIMutI CAGTG 2 cut(s) 218, 340
Cac8I GCNNGC 3 cut(s) 172, 249, 744
Cfr13I GGNCC 2 cut(s) 277, 740
CseI GACGC 1 cut(s) 458
CviAII CATG 7 cut(s) 80, 202, 244, 253, 318, 625, 688
CviJI RGCY 9 cut(s) 29, 62, 120, 170, 311, 530, 742, 752, 782
CviKI_1 RGCY 9 cut(s) 29, 62, 120, 170, 311, 530, 742, 752, 782
DdeI CTNAG 2 cut(s) 137, 747
DpnI GATC 3 cut(s) 206, 549, 616
DpnII GATC 3 cut(s) 204, 547, 614
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 277
EcoO109I RGGNCCY 2 cut(s) 277, 740
EcoRI GAATTC 1 cut(s) 604
EcoT22I ATGCAT 1 cut(s) 294
FaeI CATG 7 cut(s) 83, 205, 247, 256, 321, 628, 691
FaqI GGGAC 2 cut(s) 116, 263
FatI CATG 7 cut(s) 79, 201, 243, 252, 317, 624, 687
FauI CCCGC 1 cut(s) 730
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 60, 594
Fsp4HI GCNGC 2 cut(s) 60, 594
FspBI CTAG 2 cut(s) 312, 832
GluI GCNGC 2 cut(s) 60, 594
GsaI CCCAGC 1 cut(s) 756
HaeIII GGCC 1 cut(s) 742
HgaI GACGC 1 cut(s) 458
Hin1I GRCGYC 1 cut(s) 469
Hin1II CATG 7 cut(s) 83, 205, 247, 256, 321, 628, 691
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 4 cut(s) 35, 86, 180, 223
Hpy166II GTNNAC 5 cut(s) 114, 199, 230, 305, 460
Hpy188I TCNGA 4 cut(s) 138, 327, 474, 723
Hpy188III TCNNGA 5 cut(s) 39, 461, 573, 632, 650
Hpy8I GTNNAC 5 cut(s) 114, 199, 230, 305, 460
Hpy99I CGWCG 2 cut(s) 270, 474
HpyAV CCTTC 3 cut(s) 139, 268, 442
HpyCH4III ACNGT 2 cut(s) 266, 567
HpyCH4V TGCA 4 cut(s) 218, 230, 292, 371
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 248, 527, 743
HpyF3I CTNAG 2 cut(s) 137, 747
Hsp92I GRCGYC 1 cut(s) 469
Hsp92II CATG 7 cut(s) 83, 205, 247, 256, 321, 628, 691
Kzo9I GATC 3 cut(s) 204, 547, 614
LmnI GCTCC 1 cut(s) 829
Lsp1109I GCAGC 2 cut(s) 71, 580
MaeI CTAG 2 cut(s) 312, 832
MalI GATC 3 cut(s) 206, 549, 616
MboI GATC 3 cut(s) 204, 547, 614
MboII GAAGA 2 cut(s) 167, 350
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 3 cut(s) 232, 523, 826
MluCI AATT 5 cut(s) 162, 404, 552, 604, 713
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 452
MnlI CCTC 8 cut(s) 25, 144, 151, 501, 646, 700, 717, 742
Mph1103I ATGCAT 1 cut(s) 294
MroXI GAANNNNTTC 2 cut(s) 110, 456
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 2 cut(s) 623, 819
MunI CAATTG 1 cut(s) 162
MwoI GCNNNNNNNGC 4 cut(s) 167, 248, 527, 743
NdeII GATC 3 cut(s) 204, 547, 614
NlaIII CATG 7 cut(s) 83, 205, 247, 256, 321, 628, 691
NlaIV GGNNCC 2 cut(s) 279, 741
NsiI ATGCAT 1 cut(s) 294
PdmI GAANNNNTTC 2 cut(s) 110, 456
PfeI GAWTC 3 cut(s) 35, 180, 223
PkrI GCNGC 2 cut(s) 61, 595
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PpuMI RGGWCCY 1 cut(s) 277
PsiI TTATAA 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 752
PspN4I GGNNCC 2 cut(s) 279, 741
PspPI GGNCC 2 cut(s) 277, 740
PspPPI RGGWCCY 1 cut(s) 277
PsuI RGATCY 1 cut(s) 614
RseI CAYNNNNRTG 2 cut(s) 623, 819
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 2 cut(s) 60, 594
Sau3AI GATC 3 cut(s) 204, 547, 614
Sau96I GGNCC 2 cut(s) 277, 740
SchI GAGTC 1 cut(s) 95
SduI GDGCHC 3 cut(s) 232, 523, 826
SetI ASST 6 cut(s) 64, 172, 196, 279, 532, 657
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 2 cut(s) 623, 819
SmlI CTYRAG 1 cut(s) 648
SmoI CTYRAG 1 cut(s) 648
Sse9I AATT 5 cut(s) 162, 404, 552, 604, 713
SsiI CCGC 1 cut(s) 737
SspMI CTAG 2 cut(s) 312, 832
TaaI ACNGT 2 cut(s) 266, 567
TaqI TCGA 2 cut(s) 40, 198
TasI AATT 5 cut(s) 162, 404, 552, 604, 713
TfiI GAWTC 3 cut(s) 35, 180, 223
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 2 cut(s) 225, 340
TseI GCWGC 2 cut(s) 59, 593
TspDTI ATGAA 4 cut(s) 69, 402, 706, 803
TspRI CASTG 2 cut(s) 225, 340
VneI GTGCAC 1 cut(s) 228
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 2 cut(s) 604, 713
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 198
XmnI GAANNNNTTC 2 cut(s) 110, 456
XspI CTAG 2 cut(s) 312, 832
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.