pycom01g05390

phosphoglycerate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001245
Physical Location & Seq
Forward (+)
5101758 .. 5106748
4991 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05390.1

Sequence Viewer

Length: 1230 bp
ATGTTAGTTTTTGCCGTGGGAAAAGTGAACAGGAATTCCGCCACCTCTTTCTTATTCTGTGTTTCTCCTTCCCCACTTCCCCCGCTAATTTGTAAAAACACCCATATCTCTCTCCATCTCTCTCCATCTCTCTCTCTCTTAAGGCATCGTTCCCTCCAAATCTTTGCAGATCGAGTCCACTCGCAGATTCCCCACAGACCCATCCTCCATTCATGTGACAGAATGGTTGCGACAGCTTTTCGCCATCCTGTTGGAATCTTTCACCTCCATTGTTCTGTTGGTGAGACTGGCTCTGCCAGAAATTGTAGGCAGTCATCTTTCAGATTTATCTCTAAAAATTTTGCGGTCGATGTTCGCCCGCTTAGAAAAGAACATTGTAGCTCTAGACAGTGTAAACTGCACGTAGTTTGTTCATCGTCTTCCCAAGCTTCGACAACTGATCCAATTTTGTCTACCTCGCAAGGTGAAAGTTCTTTGGTCTTAATTCGTCATGGCGAATCATTGTGGAATGAGAAGAACTTGTTCACCGGTTGTGTTGATGTGCCTTTAACCAAGAGAGGTGTTGATGAAGCAATAGAAGCTGGTAAGAGAATTAGCAACATCCCTGTAGATACGATTTACACATCTTTGCTAATTCGTGCACAGATGACGGCTATGCTTGCCATGACTCAACACCGCCGGAAGAAGGTGCCAATCATTGTGCATAACGAGAGTAGACAGGCGGAAACATGGAGTCAAATTTACAGTGGAGATACTAAAAAGCAATCAATTCCAGTTATAACTGCTTGGCAATTAAATGAAAGAATGTATGGTGAATTACAAGGTCTTAATAAGCTGGAGACATCTGAGAGATATGGAAAAGAGAAAGTGCATGAGTGGCGTCGGAGTTATGATATTCCTCCCCCCAGTGGTGAGAGTTTGGAAATGTGTTCTCAAAGAGCTGTAGCCTATTTTAGAGAACAGATTGAACCACAACTACAATCTGGAAAGAACGTTCTGGTTGCTGCTCACGGAAATTCATTGAGGTCTATTATTATGTATCTCGAGAGACTAACTTCTCAAGAGGTTATTAGGTTAGAGTTATCAACCGGAGTACCACTGCTTTATATTTATAAGGAGGGAGAGTTTATGAGGAGGGGAAGTCCAGTTGGACCTACAGAACCTGGTGTTTATGCGTATACTAAGAGTTTAGCACTTTACAGGCAAGAATTGGATAAAAGGTTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00260 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko05230 map00010 map00260 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map05230
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

410

Amino Acids

46.21

Weight (kDa)

9.49

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 159 - 235 2.4e-17 Histidine phosphatase superfamily (branch 1)
His_Phos_1 PF00300 254 - 363 4.3e-15 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 779, 1113
AccB1I GGYRCC 1 cut(s) 688
AccI GTMKAC 3 cut(s) 452, 715, 1178
AciI CCGC 6 cut(s) 39, 83, 344, 359, 676, 722
AclI AACGTT 1 cut(s) 993
AclWI GGATC 1 cut(s) 434
AcsI RAATTY 4 cut(s) 34, 337, 738, 1015
AcyI GRCGYC 1 cut(s) 880
AfaI GTAC 1 cut(s) 1095
AfiI CCNNNNNNNGG 2 cut(s) 685, 908
AflII CTTAAG 1 cut(s) 139
AgeI ACCGGT 1 cut(s) 527
AgsI TTSAA 1 cut(s) 968
AjnI CCWGG 1 cut(s) 1162
AloI GAACNNNNNNTCC 4 cut(s) 20, 52, 978, 1010
AluBI AGCT 6 cut(s) 236, 381, 428, 581, 835, 941
AluI AGCT 6 cut(s) 236, 381, 428, 581, 835, 941
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 3 cut(s) 278, 833, 1042
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 1 cut(s) 434
AlwNI CAGNNNCTG 1 cut(s) 1163
Ama87I CYCGRG 1 cut(s) 1043
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 1 cut(s) 1004
ApoI RAATTY 4 cut(s) 34, 337, 738, 1015
AsiGI ACCGGT 1 cut(s) 527
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 1151
AsuHPI GGTGA 6 cut(s) 254, 293, 476, 517, 824, 923
AvaI CYCGRG 1 cut(s) 1043
AvaII GGWCC 1 cut(s) 1151
BaeGI GKGCMC 1 cut(s) 643
BaeI ACNNNNGTAYC 2 cut(s) 1077, 1110
BanI GGYRCC 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 1 cut(s) 991
BccI CCATC 4 cut(s) 123, 133, 209, 252
BceAI ACGGC 1 cut(s) 666
BciT130I CCWGG 1 cut(s) 1164
BcoDI GTCTC 3 cut(s) 278, 833, 1042
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 3 cut(s) 606, 942, 1155
BfrI CTTAAG 1 cut(s) 139
BisI GCNGC 1 cut(s) 1005
BlsI GCNGC 1 cut(s) 1006
Bme1390I CCNGG 1 cut(s) 1164
Bme18I GGWCC 1 cut(s) 1151
BmeT110I CYCGRG 1 cut(s) 1043
BmgT120I GGNCC 1 cut(s) 1151
BmiI GGNNCC 1 cut(s) 690
BmrFI CCNGG 1 cut(s) 1164
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 900
BpiI GAAGAC 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpmI CTGGAG 1 cut(s) 857
BpuEI CTTGAG 1 cut(s) 1044
BsaAI YACGTR 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 880
BsaJI CCNNGG 1 cut(s) 15
BsaWI WCCGGW 2 cut(s) 527, 1088
BsaXI ACNNNNNCTCC 6 cut(s) 189, 219, 1109, 1126, 1139, 1156
Bsc4I CCNNNNNNNGG 2 cut(s) 685, 908
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 4 cut(s) 292, 773, 906, 1145
BseBI CCWGG 1 cut(s) 1164
BseDI CCNNGG 1 cut(s) 15
BseGI GGATG 3 cut(s) 201, 244, 600
BseLI CCNNNNNNNGG 2 cut(s) 685, 908
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 4 cut(s) 292, 773, 906, 1145
BseRI GAGGAG 1 cut(s) 1147
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 1 cut(s) 991
BsgI GTGCAG 1 cut(s) 383
Bsh1285I CGRYCG 1 cut(s) 348
BshNI GGYRCC 1 cut(s) 688
BshTI ACCGGT 1 cut(s) 527
BsiEI CGRYCG 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 643
BsiHKCI CYCGRG 1 cut(s) 1043
BsiSI CCGG 3 cut(s) 528, 679, 1089
BslI CCNNNNNNNGG 2 cut(s) 685, 908
BsmAI GTCTC 3 cut(s) 278, 833, 1042
BsoBI CYCGRG 1 cut(s) 1043
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 2 cut(s) 169, 439
BspACI CCGC 6 cut(s) 39, 83, 344, 359, 676, 722
BspCNI CTCAG 1 cut(s) 838
BspLI GGNNCC 1 cut(s) 690
BspPI GGATC 1 cut(s) 434
BspT107I GGYRCC 1 cut(s) 688
BspTI CTTAAG 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 527
BsrI ACTGG 4 cut(s) 292, 773, 906, 1145
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 1 cut(s) 15
BssMI GATC 2 cut(s) 169, 439
BssNAI GTATAC 1 cut(s) 1179
BssNI GRCGYC 1 cut(s) 880
Bst1107I GTATAC 1 cut(s) 1179
Bst2UI CCWGG 1 cut(s) 1164
Bst4CI ACNGT 2 cut(s) 390, 746
BstACI GRCGYC 1 cut(s) 880
BstAFI CTTAAG 1 cut(s) 139
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 2 cut(s) 359, 660
BstDEI CTNAG 3 cut(s) 362, 846, 1182
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 3 cut(s) 201, 244, 600
BstKTI GATC 2 cut(s) 172, 442
BstMAI GTCTC 3 cut(s) 278, 833, 1042
BstMBI GATC 2 cut(s) 169, 439
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 3 cut(s) 578, 659, 877
BstNI CCWGG 1 cut(s) 1164
BstSCI CCNGG 1 cut(s) 1162
BstSFI CTRYAG 3 cut(s) 606, 942, 1155
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 1 cut(s) 991
BstV2I GAAGAC 1 cut(s) 411
BstXI CCANNNNNNTGG 1 cut(s) 251
BstZ17I GTATAC 1 cut(s) 1179
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 3 cut(s) 201, 244, 600
BtsI GCAGTG 1 cut(s) 1097
BtsIMutI CAGTG 4 cut(s) 395, 751, 913, 1097
Cac8I GCNNGC 2 cut(s) 359, 660
CaiI CAGNNNCTG 1 cut(s) 1163
Cfr10I RCCGGY 1 cut(s) 527
Cfr13I GGNCC 1 cut(s) 1151
CseI GACGC 1 cut(s) 869
CsiI ACCWGGT 1 cut(s) 1162
Csp6I GTAC 1 cut(s) 1094
CspAI ACCGGT 1 cut(s) 527
CviAII CATG 5 cut(s) 213, 491, 664, 729, 872
CviJI RGCY 9 cut(s) 236, 291, 381, 428, 581, 653, 835, 941, 947
CviKI_1 RGCY 9 cut(s) 236, 291, 381, 428, 581, 653, 835, 941, 947
CviQI GTAC 1 cut(s) 1094
DdeI CTNAG 3 cut(s) 362, 846, 1182
DpnI GATC 2 cut(s) 171, 441
DpnII GATC 2 cut(s) 169, 439
EciI GGCGGA 2 cut(s) 28, 737
Eco47I GGWCC 1 cut(s) 1151
Eco88I CYCGRG 1 cut(s) 1043
EcoRI GAATTC 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 1162
FaeI CATG 5 cut(s) 216, 494, 667, 732, 875
FalI AAGNNNNNCTT 1 cut(s) 1210
FatI CATG 5 cut(s) 212, 490, 663, 728, 871
FauI CCCGC 2 cut(s) 90, 366
FblI GTMKAC 3 cut(s) 452, 715, 1178
Fnu4HI GCNGC 1 cut(s) 1005
FokI GGATG 3 cut(s) 188, 231, 587
Fsp4HI GCNGC 1 cut(s) 1005
FspBI CTAG 1 cut(s) 384
GluI GCNGC 1 cut(s) 1005
GsuI CTGGAG 1 cut(s) 857
HapII CCGG 3 cut(s) 528, 679, 1089
HgaI GACGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 880
Hin1II CATG 5 cut(s) 216, 494, 667, 732, 875
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 6 cut(s) 174, 187, 255, 497, 667, 733
HpaII CCGG 3 cut(s) 528, 679, 1089
HphI GGTGA 6 cut(s) 254, 293, 476, 517, 824, 923
Hpy166II GTNNAC 8 cut(s) 28, 178, 395, 453, 525, 641, 716, 1179
Hpy188I TCNGA 3 cut(s) 323, 847, 885
Hpy188III TCNNGA 5 cut(s) 384, 984, 1043, 1045, 1061
Hpy8I GTNNAC 8 cut(s) 28, 178, 395, 453, 525, 641, 716, 1179
Hpy99I CGWCG 1 cut(s) 885
HpyAV CCTTC 2 cut(s) 78, 679
HpyCH4III ACNGT 2 cut(s) 390, 746
HpyCH4IV ACGT 2 cut(s) 402, 993
HpyCH4V TGCA 5 cut(s) 167, 400, 641, 703, 871
HpyF10VI GCNNNNNNNGC 3 cut(s) 578, 659, 877
HpyF3I CTNAG 3 cut(s) 362, 846, 1182
HpySE526I ACGT 2 cut(s) 402, 993
Hsp92I GRCGYC 1 cut(s) 880
Hsp92II CATG 5 cut(s) 216, 494, 667, 732, 875
Kzo9I GATC 2 cut(s) 169, 439
Lsp1109I GCAGC 1 cut(s) 991
LweI GCATC 1 cut(s) 154
MabI ACCWGGT 1 cut(s) 1162
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 402, 993
MaeIII GTNAC 1 cut(s) 215
MalI GATC 2 cut(s) 171, 441
MboI GATC 2 cut(s) 169, 439
MboII GAAGA 3 cut(s) 411, 526, 694
MhlI GDGCHC 1 cut(s) 643
MlyI GAGTC 3 cut(s) 183, 661, 742
MmeI TCCRAC 3 cut(s) 232, 863, 1129
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 5 cut(s) 140, 482, 548, 794, 828
MslI CAYNNNNRTG 1 cut(s) 213
MspCI CTTAAG 1 cut(s) 139
MspI CCGG 3 cut(s) 528, 679, 1089
MspR9I CCNGG 1 cut(s) 1164
MvaI CCWGG 1 cut(s) 1164
MwoI GCNNNNNNNGC 3 cut(s) 578, 659, 877
NdeII GATC 2 cut(s) 169, 439
NlaIII CATG 5 cut(s) 216, 494, 667, 732, 875
NlaIV GGNNCC 1 cut(s) 690
NmuCI GTSAC 1 cut(s) 215
PaeR7I CTCGAG 1 cut(s) 1043
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 3 cut(s) 187, 255, 497
PinAI ACCGGT 1 cut(s) 527
PkrI GCNGC 1 cut(s) 1006
PleI GAGTC 3 cut(s) 182, 661, 741
PpsI GAGTC 3 cut(s) 182, 661, 741
Ppu21I YACGTR 1 cut(s) 403
PsiI TTATAA 2 cut(s) 779, 1113
Psp1406I AACGTT 1 cut(s) 993
Psp6I CCWGG 1 cut(s) 1162
PspGI CCWGG 1 cut(s) 1162
PspN4I GGNNCC 1 cut(s) 690
PspPI GGNCC 1 cut(s) 1151
PsrI GAACNNNNNNTAC 2 cut(s) 960, 992
PstNI CAGNNNCTG 1 cut(s) 1163
RsaI GTAC 1 cut(s) 1095
RsaNI GTAC 1 cut(s) 1094
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 5 cut(s) 140, 482, 548, 794, 828
SatI GCNGC 1 cut(s) 1005
Sau3AI GATC 2 cut(s) 169, 439
Sau96I GGNCC 1 cut(s) 1151
SchI GAGTC 3 cut(s) 183, 661, 742
ScrFI CCNGG 1 cut(s) 1164
SduI GDGCHC 1 cut(s) 643
SexAI ACCWGGT 1 cut(s) 1162
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 3 cut(s) 606, 942, 1155
Sfr274I CTCGAG 1 cut(s) 1043
SinI GGWCC 1 cut(s) 1151
SlaI CTCGAG 1 cut(s) 1043
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 3 cut(s) 139, 1043, 1059
SmoI CTYRAG 3 cut(s) 139, 1043, 1059
SsiI CCGC 6 cut(s) 39, 83, 344, 359, 676, 722
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 1162
TaaI ACNGT 2 cut(s) 390, 746
TaiI ACGT 2 cut(s) 405, 996
TaqI TCGA 4 cut(s) 172, 348, 431, 1044
TfiI GAWTC 3 cut(s) 187, 255, 497
Tru1I TTAA 5 cut(s) 140, 482, 548, 794, 828
Tru9I TTAA 5 cut(s) 140, 482, 548, 794, 828
TscAI CASTG 4 cut(s) 395, 751, 913, 1104
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 1004
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 5 cut(s) 201, 402, 582, 813, 1008
TspGWI ACGGA 1 cut(s) 1026
TspRI CASTG 4 cut(s) 395, 751, 913, 1104
Vha464I CTTAAG 1 cut(s) 139
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 1 cut(s) 1151
XapI RAATTY 4 cut(s) 34, 337, 738, 1015
XbaI TCTAGA 1 cut(s) 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
XhoI CTCGAG 1 cut(s) 1043
XmiI GTMKAC 3 cut(s) 452, 715, 1178
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.