MD15G1398400.v1.1
WRKY Family

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008836
Physical Location & Seq
Reverse (-)
49918231 .. 49919303
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1398400.v1.1.491

Sequence Viewer

Length: 993 bp
ATGTCTCTTCATGTCGAAAGTTGCCATCAGATGCGTAGTTTGATGAACTACACGGCCACAAATTTGGTTCCGAATAAGCTCGTGTTCAAGAACTTGGAAGAGTTGTGTGTGAGTAACATGAATGAGTTAAAAGAGTTATGTGTTGGCGACTTACCAGATGGATCTCTAGGCAATCTGACGCTATTGGAAGTGAAAAAGTGTCACGCCTTTGCGAATGCACTTCTCCAATCAAATTTGTTAGAGAAACTCGAGAATCTGGAAGTACTTATTGTTGATGGTATGAAAAGTTTGGAATACGTGTTTCAATCCGATGGTCTCAAACCGGAGCAAAGTGTCATGGGAAAATGCAGAGAGATGAAGCTGGAGGATCTACCAGCACTGAGAAACATATGGAATGGCCCTGCGCAACACGCGACATTCCATAATCTTAAAATTTTGACAGTTGCATGGTGTATGAAACTGAAGAACATCTTCACAGCGGATATGTCTCGGTGTCTTTCACAACTGGAAGAGCTGTGGCTTTCCGAGTGCGATTGCTTGGAGACAATTATCGGACCAAGCGAGGGAACATTGAACAGCAAGATCATTTTGCCGCAACTAAACTACTTATCGTTGCAGCGTCTTCCACGGTTCAAGAGCTTTTATAGCGGTGCAAGTACTGATGTCGAGTGCCCTTCAATGGAGCATTTGTATGTACACTACTGCCCGGAATATTTAATCTCTACGTCGGACTTCAACAGCCGGAAGGAAGTCCAAGTAAATGCAGACCAGCAAGTGCTCTTTGGCCCCAGAAAAATAAAGGCTTCGTACTCGATGACCAGAGAGACTCCTACATTGATTGACGACGGCTATGCGTGGAAGAATTGCGGACAAGATGAGATCCCTGGTTCTGATCACCCAAGGTATAATACCACTTTTTTACACAATTTTTGCACACATTTTTGTAAGGCCCATTCTGTGATGTATTTAGCAATCCAAACCGTCTATCTTTAA

Protein Analysis

331

Amino Acids

37.58

Weight (kDa)

5.78

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 32 - 178 4.9e-30 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 405
AccII CGCG 1 cut(s) 413
AciI CCGC 4 cut(s) 479, 593, 648, 867
AclWI GGATC 3 cut(s) 169, 375, 874
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 3 cut(s) 61, 232, 432
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 4 cut(s) 264, 658, 696, 809
AfiI CCNNNNNNNGG 2 cut(s) 563, 679
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 6 cut(s) 88, 305, 574, 634, 678, 736
AjnI CCWGG 1 cut(s) 883
AluBI AGCT 4 cut(s) 79, 361, 514, 639
AluI AGCT 4 cut(s) 79, 361, 514, 639
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 5 cut(s) 9, 320, 492, 536, 818
AlwI GGATC 3 cut(s) 169, 375, 874
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 4 cut(s) 54, 397, 784, 948
ApeKI GCWGC 1 cut(s) 616
ApoI RAATTY 3 cut(s) 61, 232, 432
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 4 cut(s) 398, 554, 785, 949
AsuC2I CCSGG 1 cut(s) 707
AsuHPI GGTGA 1 cut(s) 887
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 674
BauI CACGAG 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 1 cut(s) 628
BccI CCATC 4 cut(s) 33, 152, 269, 305
BceAI ACGGC 2 cut(s) 69, 862
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 1 cut(s) 885
BclI TGATCA 1 cut(s) 892
BcnI CCSGG 1 cut(s) 707
BcoDI GTCTC 5 cut(s) 9, 320, 492, 536, 818
BfaI CTAG 1 cut(s) 167
BisI GCNGC 2 cut(s) 593, 617
BlsI GCNGC 2 cut(s) 594, 618
BmcAI AGTACT 2 cut(s) 264, 658
Bme1390I CCNGG 2 cut(s) 707, 885
Bme18I GGWCC 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 4 cut(s) 398, 554, 785, 949
BmiI GGNNCC 2 cut(s) 69, 787
BmrFI CCNGG 2 cut(s) 707, 885
BmsI GCATC 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 614
BpmI CTGGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 707
BsaAI YACGTR 1 cut(s) 298
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 3 cut(s) 626, 883, 899
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 674, 704
Bsc4I CCNNNNNNNGG 2 cut(s) 563, 679
Bse1I ACTGG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 885
BseDI CCNNGG 3 cut(s) 626, 883, 899
BseLI CCNNNNNNNGG 2 cut(s) 563, 679
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 510
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 1 cut(s) 628
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 4 cut(s) 56, 399, 786, 950
BsiHKAI GWGCWC 1 cut(s) 780
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 323, 707, 742
BslI CCNNNNNNNGG 2 cut(s) 563, 679
BsmAI GTCTC 5 cut(s) 9, 320, 492, 536, 818
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 4 cut(s) 56, 399, 786, 950
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 674, 780
Bsp1407I TGTACA 1 cut(s) 694
Bsp143I GATC 5 cut(s) 161, 367, 582, 879, 892
BspACI CCGC 4 cut(s) 479, 593, 648, 867
BspANI GGCC 4 cut(s) 56, 399, 786, 950
BspCNI CTCAG 1 cut(s) 372
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 2 cut(s) 69, 787
BspPI GGATC 3 cut(s) 169, 375, 874
BspQI GCTCTTC 1 cut(s) 504
BspTNI GGTCTC 1 cut(s) 320
BsrGI TGTACA 1 cut(s) 694
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 3 cut(s) 626, 883, 899
BssMI GATC 5 cut(s) 161, 367, 582, 879, 892
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 899
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 3 cut(s) 442, 630, 982
Bst6I CTCTTC 3 cut(s) 12, 93, 504
BstAUI TGTACA 1 cut(s) 694
BstBAI YACGTR 1 cut(s) 298
BstDEI CTNAG 1 cut(s) 380
BstDSI CCRYGG 1 cut(s) 626
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 5 cut(s) 164, 370, 585, 882, 895
BstMAI GTCTC 5 cut(s) 9, 320, 492, 536, 818
BstMBI GATC 5 cut(s) 161, 367, 582, 879, 892
BstMWI GCNNNNNNNGC 2 cut(s) 410, 645
BstNI CCWGG 1 cut(s) 885
BstSCI CCNGG 2 cut(s) 705, 883
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 628
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 3 cut(s) 161, 367, 879
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 3 cut(s) 161, 367, 879
BsuRI GGCC 4 cut(s) 56, 399, 786, 950
BtgI CCRYGG 1 cut(s) 626
BtsIMutI CAGTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 406
Cfr13I GGNCC 4 cut(s) 398, 554, 785, 949
CseI GACGC 2 cut(s) 187, 608
Csp6I GTAC 4 cut(s) 263, 657, 695, 808
CviAII CATG 4 cut(s) 11, 118, 337, 447
CviQI GTAC 4 cut(s) 263, 657, 695, 808
DdeI CTNAG 1 cut(s) 380
DpnI GATC 5 cut(s) 163, 369, 584, 881, 894
DpnII GATC 5 cut(s) 161, 367, 582, 879, 892
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 3 cut(s) 12, 93, 504
EarI CTCTTC 3 cut(s) 12, 93, 504
Eco130I CCWWGG 1 cut(s) 899
Eco31I GGTCTC 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 899
ErhI CCWWGG 1 cut(s) 899
FaeI CATG 4 cut(s) 14, 121, 340, 450
FalI AAGNNNNNCTT 2 cut(s) 455, 487
FatI CATG 4 cut(s) 10, 117, 336, 446
FauNDI CATATG 1 cut(s) 389
FbaI TGATCA 1 cut(s) 892
Fnu4HI GCNGC 2 cut(s) 593, 617
Fsp4HI GCNGC 2 cut(s) 593, 617
FspBI CTAG 1 cut(s) 167
FspI TGCGCA 1 cut(s) 405
GlaI GCGC 1 cut(s) 405
GluI GCNGC 2 cut(s) 593, 617
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 4 cut(s) 56, 399, 786, 950
HapII CCGG 3 cut(s) 323, 707, 742
HgaI GACGC 2 cut(s) 187, 608
HhaI GCGC 1 cut(s) 406
Hin1II CATG 4 cut(s) 14, 121, 340, 450
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HinfI GANTC 2 cut(s) 253, 826
HpaII CCGG 3 cut(s) 323, 707, 742
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 1 cut(s) 697
Hpy188I TCNGA 8 cut(s) 30, 72, 177, 310, 526, 554, 730, 892
Hpy188III TCNNGA 4 cut(s) 88, 250, 257, 634
Hpy8I GTNNAC 1 cut(s) 697
Hpy99I CGWCG 2 cut(s) 730, 848
HpyAV CCTTC 2 cut(s) 684, 739
HpyCH4III ACNGT 3 cut(s) 442, 630, 982
HpyCH4IV ACGT 2 cut(s) 297, 725
HpyCH4V TGCA 7 cut(s) 218, 348, 446, 616, 653, 764, 933
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 645
HpyF3I CTNAG 1 cut(s) 380
HpySE526I ACGT 2 cut(s) 297, 725
Hsp92II CATG 4 cut(s) 14, 121, 340, 450
HspAI GCGC 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 892
Kzo9I GATC 5 cut(s) 161, 367, 582, 879, 892
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 2 cut(s) 325, 682
Lsp1109I GCAGC 1 cut(s) 628
LweI GCATC 1 cut(s) 21
MaeI CTAG 1 cut(s) 167
MaeII ACGT 2 cut(s) 297, 725
MaeIII GTNAC 2 cut(s) 113, 200
MalI GATC 5 cut(s) 163, 369, 584, 881, 894
MboI GATC 5 cut(s) 161, 367, 582, 879, 892
MboII GAAGA 6 cut(s) 110, 463, 475, 521, 614, 871
MflI RGATCY 3 cut(s) 161, 367, 879
MhlI GDGCHC 2 cut(s) 674, 780
MluCI AATT 6 cut(s) 61, 232, 432, 546, 862, 925
MlyI GAGTC 1 cut(s) 820
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 2 cut(s) 358, 556
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 4 cut(s) 128, 429, 716, 991
MslI CAYNNNNRTG 1 cut(s) 690
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 3 cut(s) 323, 707, 742
MspR9I CCNGG 2 cut(s) 707, 885
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 2 cut(s) 410, 645
NciI CCSGG 1 cut(s) 707
NdeI CATATG 1 cut(s) 389
NdeII GATC 5 cut(s) 161, 367, 582, 879, 892
NlaIII CATG 4 cut(s) 14, 121, 340, 450
NlaIV GGNNCC 2 cut(s) 69, 787
NmuCI GTSAC 1 cut(s) 200
NsbI TGCGCA 1 cut(s) 405
PaeR7I CTCGAG 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PctI GAATGC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 2 cut(s) 594, 618
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
Ppu21I YACGTR 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 883
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 2 cut(s) 69, 787
PspPI GGNCC 4 cut(s) 398, 554, 785, 949
PsuI RGATCY 3 cut(s) 161, 367, 879
RsaI GTAC 4 cut(s) 264, 658, 696, 809
RsaNI GTAC 4 cut(s) 263, 657, 695, 808
RseI CAYNNNNRTG 1 cut(s) 690
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 4 cut(s) 128, 429, 716, 991
SatI GCNGC 2 cut(s) 593, 617
Sau3AI GATC 5 cut(s) 161, 367, 582, 879, 892
Sau96I GGNCC 4 cut(s) 398, 554, 785, 949
ScaI AGTACT 2 cut(s) 264, 658
SchI GAGTC 1 cut(s) 820
ScrFI CCNGG 2 cut(s) 707, 885
SduI GDGCHC 2 cut(s) 674, 780
SetI ASST 7 cut(s) 81, 300, 363, 516, 641, 728, 905
SfaNI GCATC 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 248
SinI GGWCC 1 cut(s) 554
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 690
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 6 cut(s) 61, 232, 432, 546, 862, 925
SsiI CCGC 4 cut(s) 479, 593, 648, 867
SspI AATATT 1 cut(s) 713
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 2 cut(s) 705, 883
StyI CCWWGG 1 cut(s) 899
TaaI ACNGT 3 cut(s) 442, 630, 982
TaiI ACGT 2 cut(s) 300, 728
TaqI TCGA 4 cut(s) 15, 249, 666, 812
TasI AATT 6 cut(s) 61, 232, 432, 546, 862, 925
TatI WGTACW 3 cut(s) 262, 656, 694
TauI GCSGC 1 cut(s) 595
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 4 cut(s) 128, 429, 716, 991
Tru9I TTAA 4 cut(s) 128, 429, 716, 991
TscAI CASTG 1 cut(s) 384
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 1 cut(s) 616
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 5 cut(s) 59, 134, 296, 371, 470
TspRI CASTG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 554
XapI RAATTY 3 cut(s) 61, 232, 432
XhoI CTCGAG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 1 cut(s) 167
ZrmI AGTACT 2 cut(s) 264, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.