RchiOBHm_Chr5g0079581

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008836
Physical Location & Seq
Reverse (-)
85329704 .. 85333590
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35397

Sequence Viewer

Length: 498 bp
ATGCAGTTGTTGGGTCCAAATTCAGTGAAGAGCATTTGTAGTGGTCCTGCCCCATGCGTAATGTTCCAGAGTCTTAAAAGTTTGACCTTTTACCGTTGCGAGTTGCTGCAAAGTCTGTTCGCATATGATGTAGCCCAGAATCTTGTTCACTTGGAAGACCTTTTAGTAGAATGGTGCCCTTTGTTGGAAAGAGTAATTGAAGCAGTGAACAACGAGAAGACAGTTCTTCAAAAAATGAAGAACTTGGTTTTCATGGGACTTCCCAAGTTGTATGGTTCAAGTGCTGCTGTTGACATTGAGTGTCCTTCATTGGAACACTTGATAGTGGTGGATTGTCCCCGGTTTTCATTTTCAACCTCTTCCTTAAGCAGGAACCAGTTTTCATTTTCAACCCCTGCTTCTGACTACCTTGGCAGCAGGAACCTAGTCAAACTCAATGATCAACAACATCTTCAACTTCTATCATCAAGGTTGATGAACTTGTGCAGGCTCAGTTAA

Protein Analysis

165

Amino Acids

18.53

Weight (kDa)

6.8

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 7 - 114 1.5e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 174
AcsI RAATTY 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 184, 369
AflII CTTAAG 1 cut(s) 364
AgsI TTSAA 6 cut(s) 200, 230, 279, 354, 390, 455
ApeKI GCWGC 3 cut(s) 106, 284, 414
ApoI RAATTY 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 14, 44
AsuC2I CCSGG 1 cut(s) 340
AvaII GGWCC 2 cut(s) 14, 44
BaeGI GKGCMC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 174
BbsI GAAGAC 2 cut(s) 162, 224
BbvI GCAGC 3 cut(s) 93, 271, 426
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 1 cut(s) 340
BfaI CTAG 1 cut(s) 425
BfrI CTTAAG 1 cut(s) 364
BisI GCNGC 3 cut(s) 107, 285, 415
BlsI GCNGC 3 cut(s) 108, 286, 416
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 2 cut(s) 14, 44
BmgT120I GGNCC 2 cut(s) 14, 44
BmiI GGNNCC 4 cut(s) 15, 176, 374, 422
BmrFI CCNGG 1 cut(s) 340
BpiI GAAGAC 2 cut(s) 162, 224
BpuMI CCSGG 1 cut(s) 340
BsaJI CCNNGG 2 cut(s) 338, 409
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 369
Bse1I ACTGG 1 cut(s) 376
BseDI CCNNGG 2 cut(s) 338, 409
BseLI CCNNNNNNNGG 2 cut(s) 184, 369
BseNI ACTGG 1 cut(s) 376
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 3 cut(s) 93, 271, 426
BshNI GGYRCC 1 cut(s) 174
BsiSI CCGG 1 cut(s) 340
BslFI GGGAC 2 cut(s) 270, 321
BslI CCNNNNNNNGG 2 cut(s) 184, 369
BsmFI GGGAC 2 cut(s) 270, 321
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 1 cut(s) 439
BspLI GGNNCC 4 cut(s) 15, 176, 374, 422
BspQI GCTCTTC 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 174
BspTI CTTAAG 1 cut(s) 364
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 2 cut(s) 338, 409
BssMI GATC 1 cut(s) 439
BssT1I CCWWGG 1 cut(s) 409
Bst4CI ACNGT 2 cut(s) 95, 223
Bst6I CTCTTC 2 cut(s) 23, 364
BstAFI CTTAAG 1 cut(s) 364
BstC8I GCNNGC 1 cut(s) 488
BstDEI CTNAG 1 cut(s) 491
BstENI CCTNNNNNAGG 1 cut(s) 367
BstKTI GATC 1 cut(s) 442
BstMBI GATC 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 338
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 93, 271, 426
BstV2I GAAGAC 2 cut(s) 162, 224
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 30, 210
Cac8I GCNNGC 1 cut(s) 488
Cfr13I GGNCC 2 cut(s) 14, 44
CviAII CATG 2 cut(s) 54, 253
CviJI RGCY 2 cut(s) 134, 490
CviKI_1 RGCY 2 cut(s) 134, 490
DdeI CTNAG 1 cut(s) 491
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
Eam1104I CTCTTC 2 cut(s) 23, 364
EarI CTCTTC 2 cut(s) 23, 364
Eco130I CCWWGG 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 14, 44
EcoNI CCTNNNNNAGG 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 2 cut(s) 57, 256
FaiI YATR 5 cut(s) 55, 124, 126, 254, 273
FaqI GGGAC 2 cut(s) 270, 321
FatI CATG 2 cut(s) 53, 252
FauNDI CATATG 1 cut(s) 124
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 3 cut(s) 107, 285, 415
Fsp4HI GCNGC 3 cut(s) 107, 285, 415
FspBI CTAG 1 cut(s) 425
GluI GCNGC 3 cut(s) 107, 285, 415
HapII CCGG 1 cut(s) 340
Hin1II CATG 2 cut(s) 57, 256
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 70, 139
HpaII CCGG 1 cut(s) 340
Hpy166II GTNNAC 3 cut(s) 148, 208, 292
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 3 cut(s) 148, 208, 292
HpyAV CCTTC 1 cut(s) 315
HpyCH4III ACNGT 2 cut(s) 95, 223
HpyCH4V TGCA 3 cut(s) 4, 109, 486
HpyF3I CTNAG 1 cut(s) 491
Hsp92II CATG 2 cut(s) 57, 256
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 1 cut(s) 439
LguI GCTCTTC 1 cut(s) 23
LpnPI CCDG 9 cut(s) 60, 80, 149, 353, 355, 389, 403, 408, 472
Lsp1109I GCAGC 3 cut(s) 93, 271, 426
MaeI CTAG 1 cut(s) 425
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MboII GAAGA 7 cut(s) 40, 167, 218, 229, 250, 351, 443
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 2 cut(s) 19, 195
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 1 cut(s) 367
MseI TTAA 3 cut(s) 75, 365, 496
MspCI CTTAAG 1 cut(s) 364
MspI CCGG 1 cut(s) 340
MspR9I CCNGG 1 cut(s) 340
NciI CCSGG 1 cut(s) 340
NdeI CATATG 1 cut(s) 124
NdeII GATC 1 cut(s) 439
NlaIII CATG 2 cut(s) 57, 256
NlaIV GGNNCC 4 cut(s) 15, 176, 374, 422
PciSI GCTCTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 3 cut(s) 108, 286, 416
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PspN4I GGNNCC 4 cut(s) 15, 176, 374, 422
PspPI GGNCC 2 cut(s) 14, 44
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 75, 365, 496
SatI GCNGC 3 cut(s) 107, 285, 415
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 2 cut(s) 14, 44
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 340
SduI GDGCHC 1 cut(s) 179
SetI ASST 6 cut(s) 89, 162, 359, 411, 426, 473
SinI GGWCC 2 cut(s) 14, 44
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
Sse9I AATT 2 cut(s) 19, 195
SspMI CTAG 1 cut(s) 425
StyD4I CCNGG 1 cut(s) 338
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 95, 223
TasI AATT 2 cut(s) 19, 195
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 3 cut(s) 75, 365, 496
Tru9I TTAA 3 cut(s) 75, 365, 496
TscAI CASTG 2 cut(s) 30, 210
TseI GCWGC 3 cut(s) 106, 284, 414
TspDTI ATGAA 6 cut(s) 241, 251, 297, 336, 372, 491
TspRI CASTG 2 cut(s) 30, 210
Vha464I CTTAAG 1 cut(s) 364
VpaK11BI GGWCC 2 cut(s) 14, 44
XagI CCTNNNNNAGG 1 cut(s) 367
XapI RAATTY 1 cut(s) 19
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.