Rroxscaffold_2G00110810

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008836
Physical Location & Seq
Reverse (-)
35300406 .. 35300861
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00110810.1

Sequence Viewer

Length: 456 bp
ATGAGATTGTGCAGTCTACCAAAACTCATAAAGATATGGAGCGGTCATGCTCCACGTGCAATCTTCTTCAATCTTAAACTTTTGGCAGTTAGTGGATGCAAGAAGCTAAAAAGTCTCTTCACGGTCGATGTAGCTCAATGTCTTTCGCAATTGGAAGACCTTTGGGTGCATTCTTGCTTTAGGTTGGATCGAATTGTTGAAGCAAGCGAGGACACAGGGACGATGAACGTCATTTTTCCGAAATTGAAGAACTTAGTCTTGGAAGATCTTCCACGGCTCAGAAGGTTGTATAGTAACAGTAGTGGAGGTCCGACTTCATTGGCCATAGAGTTCCCTTCATTGGAACACTTCCCCACGTGGGATTGCCCCCGGTTCTCATCCTCTGCTTCTAATTTCCACAGCCAGATGCAAGTCCAAAATGATATACAACATTATAGCTTTCTAAAGAAAAGGTGA

Protein Analysis

151

Amino Acids

17.28

Weight (kDa)

9.25

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 1 - 59 3e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 16
AciI CCGC 1 cut(s) 42
AclWI GGATC 1 cut(s) 195
AcoI YGGCCR 1 cut(s) 321
AcvI CACGTG 2 cut(s) 56, 357
AfiI CCNNNNNNNGG 2 cut(s) 340, 358
AgsI TTSAA 3 cut(s) 70, 200, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 3 cut(s) 106, 134, 438
AluI AGCT 3 cut(s) 106, 134, 438
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 1 cut(s) 195
AoxI GGCC 1 cut(s) 321
Asp700I GAANNNNTTC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 308
AsuC2I CCSGG 1 cut(s) 370
AvaII GGWCC 1 cut(s) 308
BalI TGGCCA 1 cut(s) 323
BbrPI CACGTG 2 cut(s) 56, 357
BbsI GAAGAC 1 cut(s) 162
BceAI ACGGC 1 cut(s) 290
BcnI CCSGG 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 119
BglII AGATCT 1 cut(s) 265
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 308
BmrFI CCNGG 1 cut(s) 370
BmsI GCATC 2 cut(s) 86, 396
BpiI GAAGAC 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 370
BsaAI YACGTR 2 cut(s) 56, 357
BsaJI CCNNGG 2 cut(s) 272, 368
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 358
BseDI CCNNGG 2 cut(s) 272, 368
BseGI GGATG 2 cut(s) 101, 377
BseLI CCNNNNNNNGG 2 cut(s) 340, 358
BseMII CTCAG 1 cut(s) 292
BsgI GTGCAG 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 370
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 340, 358
BsmAI GTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 1 cut(s) 323
Bsp143I GATC 2 cut(s) 187, 265
BspACI CCGC 1 cut(s) 42
BspANI GGCC 1 cut(s) 323
BspCNI CTCAG 1 cut(s) 291
BspPI GGATC 1 cut(s) 195
BsrBI CCGCTC 1 cut(s) 42
BssECI CCNNGG 2 cut(s) 272, 368
BssMI GATC 2 cut(s) 187, 265
Bst4CI ACNGT 2 cut(s) 124, 299
Bst6I CTCTTC 1 cut(s) 122
BstBAI YACGTR 2 cut(s) 56, 357
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 2 cut(s) 253, 278
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 2 cut(s) 101, 377
BstKTI GATC 2 cut(s) 190, 268
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 2 cut(s) 187, 265
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 368
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 1 cut(s) 323
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 2 cut(s) 101, 377
Cac8I GCNNGC 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 308
CviAII CATG 1 cut(s) 47
CviJI RGCY 6 cut(s) 106, 134, 277, 323, 402, 438
CviKI_1 RGCY 6 cut(s) 106, 134, 277, 323, 402, 438
DdeI CTNAG 2 cut(s) 253, 278
DpnI GATC 2 cut(s) 189, 267
DpnII GATC 2 cut(s) 187, 265
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 308
Eco72I CACGTG 2 cut(s) 56, 357
FaeI CATG 1 cut(s) 50
FaiI YATR 7 cut(s) 29, 37, 48, 291, 326, 425, 435
FaqI GGGAC 1 cut(s) 232
FatI CATG 1 cut(s) 46
FblI GTMKAC 1 cut(s) 16
FokI GGATG 2 cut(s) 108, 364
HaeIII GGCC 1 cut(s) 323
HapII CCGG 1 cut(s) 370
Hin1II CATG 1 cut(s) 50
HpaII CCGG 1 cut(s) 370
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 3 cut(s) 240, 281, 312
Hpy8I GTNNAC 1 cut(s) 17
HpyAV CCTTC 2 cut(s) 276, 345
HpyCH4III ACNGT 2 cut(s) 124, 299
HpyCH4IV ACGT 3 cut(s) 55, 228, 356
HpyCH4V TGCA 5 cut(s) 12, 59, 99, 169, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 2 cut(s) 253, 278
HpySE526I ACGT 3 cut(s) 55, 228, 356
Hsp92II CATG 1 cut(s) 50
Kzo9I GATC 2 cut(s) 187, 265
LmnI GCTCC 2 cut(s) 39, 55
LpnPI CCDG 3 cut(s) 201, 383, 416
LweI GCATC 2 cut(s) 86, 396
MaeII ACGT 3 cut(s) 55, 228, 356
MaeIII GTNAC 1 cut(s) 293
MalI GATC 2 cut(s) 189, 267
MbiI CCGCTC 1 cut(s) 42
MboI GATC 2 cut(s) 187, 265
MboII GAAGA 7 cut(s) 55, 58, 109, 167, 259, 260, 275
MfeI CAATTG 1 cut(s) 149
MflI RGATCY 1 cut(s) 265
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 4 cut(s) 149, 192, 242, 391
MluNI TGGCCA 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 165, 335
MnlI CCTC 3 cut(s) 202, 299, 391
Mox20I TGGCCA 1 cut(s) 323
MroXI GAANNNNTTC 1 cut(s) 267
MscI TGGCCA 1 cut(s) 323
MseI TTAA 1 cut(s) 75
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 1 cut(s) 370
MunI CAATTG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 169
MwoI GCNNNNNNNGC 1 cut(s) 56
NciI CCSGG 1 cut(s) 370
NdeII GATC 2 cut(s) 187, 265
NlaIII CATG 1 cut(s) 50
PctI GAATGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 267
PmaCI CACGTG 2 cut(s) 56, 357
PmlI CACGTG 2 cut(s) 56, 357
Ppu21I YACGTR 2 cut(s) 56, 357
PspCI CACGTG 2 cut(s) 56, 357
PspPI GGNCC 1 cut(s) 308
PsuI RGATCY 1 cut(s) 265
SaqAI TTAA 1 cut(s) 75
Sau3AI GATC 2 cut(s) 187, 265
Sau96I GGNCC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 370
SfaNI GCATC 2 cut(s) 86, 396
SinI GGWCC 1 cut(s) 308
Sse9I AATT 4 cut(s) 149, 192, 242, 391
SsiI CCGC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 368
TaaI ACNGT 2 cut(s) 124, 299
TaiI ACGT 3 cut(s) 58, 231, 359
TaqI TCGA 2 cut(s) 126, 190
TasI AATT 4 cut(s) 149, 192, 242, 391
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TspDTI ATGAA 3 cut(s) 239, 306, 327
VpaK11BI GGWCC 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.