Rw5G042570

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008836
Physical Location & Seq
Forward (+)
74557626 .. 74558585
960 bp
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UTR
Exon/CDS
Intron
Rw5G042570.1

Sequence Viewer

Length: 450 bp
ATGAGATTGTACAATCAACATAGGGTGTTACGCATATGGAAAGGTCCTGCTCCACATGCAGTCTTCCATAGTCTTCGAAGTTTGACAGTTGAATACTACACTAAACTGAAATACCTCTTCACATCCAATGTTGCTCAATGTCTTTTGCATTTGGAAGACCTGTGGCTACAAGGTTGCAGTAGCTTGGACAGAATTATTGAAACAAGTGATGAGGAAATGATCGTCTTTCCAGAATTGAAGAACTTTATTTTGAAACATCTTCCACAGCTGAAAAGTTTTTGCAGTCCTATTTCAGTAGATATTGAATTCCCTTCATTGGAACACTTGTATGTGCGTGACTGCCCCCAGTTGATAATCTGTGCTTTTGACTTCAAAAGCAGGAACCATGTAAAACTGAATGATGAACAACATATGTCGTCTCTGTGCAGAAGGTTGTGGGATTTAGAATGA

Protein Analysis

149

Amino Acids

17.69

Weight (kDa)

6.7

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 6 - 118 1e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 305
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 6 cut(s) 92, 200, 238, 253, 305, 373
AluBI AGCT 2 cut(s) 183, 268
AluI AGCT 2 cut(s) 183, 268
Alw26I GTCTC 1 cut(s) 423
ApoI RAATTY 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 44
AsuII TTCGAA 1 cut(s) 76
AvaII GGWCC 1 cut(s) 44
BbsI GAAGAC 3 cut(s) 55, 65, 162
BcoDI GTCTC 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 383
BmrI ACTGGG 1 cut(s) 340
BmuI ACTGGG 1 cut(s) 340
BpiI GAAGAC 3 cut(s) 55, 65, 162
Bpu14I TTCGAA 1 cut(s) 76
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 346
BseGI GGATG 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 316
BseNI ACTGG 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 445
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 423
BsmBI CGTCTC 1 cut(s) 423
Bsp119I TTCGAA 1 cut(s) 76
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 383
BspT104I TTCGAA 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 9
BsrI ACTGG 1 cut(s) 346
BssMI GATC 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 1 cut(s) 122
BstAUI TGTACA 1 cut(s) 9
BstBI TTCGAA 1 cut(s) 76
BstF5I GGATG 1 cut(s) 122
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNSI RCATGY 1 cut(s) 59
BstV2I GAAGAC 3 cut(s) 55, 65, 162
BtsCI GGATG 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 10
CviAII CATG 2 cut(s) 56, 386
CviJI RGCY 3 cut(s) 166, 183, 268
CviKI_1 RGCY 3 cut(s) 166, 183, 268
CviQI GTAC 1 cut(s) 10
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 305
Esp3I CGTCTC 1 cut(s) 423
FaeI CATG 2 cut(s) 59, 389
FaiI YATR 9 cut(s) 21, 35, 37, 57, 69, 330, 387, 411, 413
FatI CATG 2 cut(s) 55, 385
FauNDI CATATG 2 cut(s) 35, 411
FokI GGATG 1 cut(s) 109
Hin1II CATG 2 cut(s) 59, 389
Hpy188III TCNNGA 1 cut(s) 230
HpyAV CCTTC 2 cut(s) 321, 423
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4V TGCA 5 cut(s) 59, 148, 177, 282, 426
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
Hsp92II CATG 2 cut(s) 59, 389
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 5 cut(s) 60, 173, 243, 359, 364
MaeIII GTNAC 2 cut(s) 27, 335
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 6 cut(s) 55, 65, 109, 167, 250, 251
MluCI AATT 3 cut(s) 192, 233, 305
MnlI CCTC 2 cut(s) 125, 205
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 268
MwoI GCNNNNNNNGC 1 cut(s) 56
NdeI CATATG 2 cut(s) 35, 411
NdeII GATC 1 cut(s) 219
NlaIII CATG 2 cut(s) 59, 389
NlaIV GGNNCC 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 1 cut(s) 59
NspV TTCGAA 1 cut(s) 76
PpuMI RGGWCCY 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 383
PspPI GGNCC 1 cut(s) 44
PspPPI RGGWCCY 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 327
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 1 cut(s) 44
SetI ASST 7 cut(s) 46, 117, 162, 175, 185, 270, 434
SfuI TTCGAA 1 cut(s) 76
SinI GGWCC 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 327
Sse9I AATT 3 cut(s) 192, 233, 305
TaaI ACNGT 1 cut(s) 88
TaqI TCGA 1 cut(s) 76
TasI AATT 3 cut(s) 192, 233, 305
TatI WGTACW 1 cut(s) 9
TseFI GTSAC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 2 cut(s) 303, 417
VpaK11BI GGWCC 1 cut(s) 44
XapI RAATTY 1 cut(s) 305
XceI RCATGY 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.