Rw5G042580

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008836
Physical Location & Seq
Reverse (-)
74560394 .. 74562459
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042580.1

Sequence Viewer

Length: 483 bp
ATGAGATTGTATGTAGGTGAAGTTGGTGAACTAACAAGCATATGGAAAGGTCCTGCTCCATATGCAATCTTCTGTAATCTTAAGCATATGAAAGTTGCTGGGTCGAACAAATTGAAATATCTCTTCACATTCGTTGTAGCTCAGCATCTCTTACAATTGGAAGACCTTTGGCTAGATGATTGTGTTAGCTTGGACAGAGTTATTGAAGCAAGCGAGGAAACAGTTAGCAACAAAACACTAATCTTTCCCGGATTGAAGAACTTACTTCTGAGAGATCTTCCACAACTGACAAGCTTCTGCACTCCAACTGGAAGTGCCACTTCAGTGGATATTGAATGTCCTTCATTGGAACACTTGTATATGCATGACTGTCCTCAATTGTTGATCTCTGCTGCCGACTTCAACAGTAGGAACCATGTACAACTTAATTCTGAACATCTGATGTCGTCTCTATACGGAAGATTGTTGAGTGGGAAGAATTAG

Protein Analysis

160

Amino Acids

17.94

Weight (kDa)

5.82

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 11 - 127 1.2e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 1 cut(s) 420
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 5 cut(s) 115, 206, 256, 335, 403
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 3 cut(s) 140, 189, 294
AluI AGCT 3 cut(s) 140, 189, 294
Alw26I GTCTC 1 cut(s) 453
ApeKI GCWGC 1 cut(s) 392
AspS9I GGNCC 1 cut(s) 50
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 2 cut(s) 29, 38
AvaII GGWCC 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 379
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 1 cut(s) 173
BfrI CTTAAG 1 cut(s) 80
BglII AGATCT 1 cut(s) 274
BisI GCNGC 1 cut(s) 393
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 168
Bpu1102I GCTNAGC 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 249
Bse1I ACTGG 1 cut(s) 313
BseMII CTCAG 2 cut(s) 155, 260
BseNI ACTGG 1 cut(s) 313
BseXI GCAGC 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 283
BsiSI CCGG 1 cut(s) 249
BsmAI GTCTC 1 cut(s) 453
BsmBI CGTCTC 1 cut(s) 453
Bsp1407I TGTACA 1 cut(s) 418
Bsp143I GATC 2 cut(s) 274, 384
Bsp1720I GCTNAGC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 154, 261
BspLI GGNNCC 1 cut(s) 413
BspTI CTTAAG 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 418
BsrI ACTGG 1 cut(s) 313
BssMI GATC 2 cut(s) 274, 384
Bst4CI ACNGT 3 cut(s) 223, 371, 407
Bst6I CTCTTC 1 cut(s) 128
BstAFI CTTAAG 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 141, 269
BstKTI GATC 2 cut(s) 277, 387
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 2 cut(s) 274, 384
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 274
BstXI CCANNNNNNTGG 1 cut(s) 325
BstYI RGATCY 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 50
Csp6I GTAC 1 cut(s) 419
CviAII CATG 2 cut(s) 365, 416
CviJI RGCY 4 cut(s) 140, 172, 189, 294
CviKI_1 RGCY 4 cut(s) 140, 172, 189, 294
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 2 cut(s) 141, 269
DpnI GATC 2 cut(s) 276, 386
DpnII GATC 2 cut(s) 274, 384
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco47I GGWCC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 306
EcoO109I RGGNCCY 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 2 cut(s) 368, 419
FalI AAGNNNNNCTT 2 cut(s) 304, 336
FatI CATG 2 cut(s) 364, 415
FauNDI CATATG 3 cut(s) 41, 61, 87
Fnu4HI GCNGC 1 cut(s) 393
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 1 cut(s) 173
GluI GCNGC 1 cut(s) 393
GsaI CCCAGC 1 cut(s) 102
HapII CCGG 1 cut(s) 249
Hin1II CATG 2 cut(s) 368, 419
HindIII AAGCTT 1 cut(s) 292
HpaII CCGG 1 cut(s) 249
HphI GGTGA 2 cut(s) 29, 38
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 3 cut(s) 270, 433, 441
Hpy8I GTNNAC 1 cut(s) 29
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 3 cut(s) 223, 371, 407
HpyCH4V TGCA 3 cut(s) 65, 300, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 2 cut(s) 141, 269
Hsp92II CATG 2 cut(s) 368, 419
Kzo9I GATC 2 cut(s) 274, 384
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 4 cut(s) 66, 84, 262, 294
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 1 cut(s) 154
MaeI CTAG 1 cut(s) 173
MalI GATC 2 cut(s) 276, 386
MboI GATC 2 cut(s) 274, 384
MboII GAAGA 6 cut(s) 61, 115, 173, 268, 269, 471
MfeI CAATTG 2 cut(s) 155, 377
MflI RGATCY 1 cut(s) 274
MluCI AATT 5 cut(s) 110, 155, 377, 427, 478
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 2 cut(s) 208, 384
Mph1103I ATGCAT 1 cut(s) 366
MseI TTAA 2 cut(s) 81, 426
MslI CAYNNNNRTG 1 cut(s) 323
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 1 cut(s) 249
MunI CAATTG 2 cut(s) 155, 377
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 249
NdeI CATATG 3 cut(s) 41, 61, 87
NdeII GATC 2 cut(s) 274, 384
NlaIII CATG 2 cut(s) 368, 419
NlaIV GGNNCC 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 366
OliI CACNNNNGTG 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 1 cut(s) 394
PpuMI RGGWCCY 1 cut(s) 50
Psp5II RGGWCCY 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 413
PspPI GGNCC 1 cut(s) 50
PspPPI RGGWCCY 1 cut(s) 50
PsuI RGATCY 1 cut(s) 274
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 2 cut(s) 81, 426
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 2 cut(s) 274, 384
Sau96I GGNCC 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 249
SetI ASST 6 cut(s) 19, 52, 142, 168, 191, 296
SfaNI GCATC 1 cut(s) 154
SinI GGWCC 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 5 cut(s) 110, 155, 377, 427, 478
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 3 cut(s) 223, 371, 407
TaqI TCGA 1 cut(s) 104
TasI AATT 5 cut(s) 110, 155, 377, 427, 478
TatI WGTACW 1 cut(s) 418
Tru1I TTAA 2 cut(s) 81, 426
Tru9I TTAA 2 cut(s) 81, 426
TscAI CASTG 1 cut(s) 330
TseI GCWGC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 104, 333
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 1 cut(s) 330
Vha464I CTTAAG 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 50
XspI CTAG 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.