Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003063

Physical Location & Seq

Genomic Coordinates Reverse (-)

6885351 .. 6888465

Gene Structure 3115 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1098600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGGCATCCCCCCATCTCCTCCTTCCATGCATGCTCCTCTCCCTATTTCTTAGTCTATTTTCTGCAGTTTTCGCTGCCGATGCATCCATCAGGTCTGCTCTCGTAAACCCTTTGAGTTGCTCTGCGAAAATCATGACATGTAACGCCTCGCTCTACCACATCAACATCGATCTGAAGGTAGAAGAAATTGCCACTTTGTACACCGTGAATCCATCCGAAATCAGACCTATAAAGCACAACAAGAAGCAAGATTACCTGATAAGTGTGCCTTGTTCTTGCAAAAACATATCCGGCACTGTTGGATACTTCTATGACACGACCTACAAGGTGAAACAATTTGACACGTTTTATAATGTGTCGACGAAGATTTACAGCGGGCAGCCTTTGTATATCGAAGAAGAGATACCGCAGTTTAAAACAGACGCTGATTTTCCCATCCATCTCCCATGCGGTTGCGTACAAAGCCAGTCTCAGATTGTGGTGACATATACAGTTCAGGAGCATGATACACTGTCGGATATTGGCACGCTACTGTCTGCGAAGATTGAAAACATTGAAAAAATGAACAAAAATATGACCGAGAATCCTTCATACATAGTTGTGGGTTGGGTGTTGTTTGTCCCCATGGAAAAGAATGGACTCAGAACATCAAAGCCAGACGGACTGGTCATATTCAGCCTTGAGGAGATTGAGGAGGCAACCGGTTACTTCGATGAAACTAAGAAAATCGGAGAGGGTGGATATGGTAGCGTGTTCCTCGGAGTAATAAGGGGGAAGGAGGTTGCCATAAAGAAGATGAAGTCTAATAGTACCAAAGAATTTTTTGCAGAGCTAAAGGTCTTGTGCAAGATCCATCACATTAACGTGGTGGAGCTTTTGGGGTATGCCAGTGGAGATGACCACCTCTACCTGGTCTATGAGTATGTCCAGAATGGATCGCTGAGCGAACATCTTCATGATCCTTTACTTAAAGGTCATCAGCCACTTTCTTGGACTGCAAGAACACAAATTGCAGTGGACGCTGCAAAAGGTATTGAGTACATTCATGACCACACAAAAGCGCGATATGTGCACCGTGATATAAAGACTAGTAACATTCTACTTGACGAGGGGTTCAGAGCAAAGGTAGCAGATTTTGGCTTGGCAAAGCTTGTTGGAAGAACCAATGAAGAAGATGTTTTCGCAACACGGCTGGTTGGAACACCGGGCTATCTTCCCCCAGAATCCGTGAAGGAGCTCCAGGTGACTCACAAAACCGATGTGTTTGCATTCGGAGTGGTACTAGCAGAGCTGATCACAGGGCAACGTGCGCTTTTCCGTGACAATCGAGAGCCTGGGAAGATGAAATCCTTAATTACAGTCATAAAAAAGGTGTTCCAAGATGATCATCCAGAAGCAGCTTTAGAGGCTGCCACAGATGGAAACCTCAGAAGCAACTATCCTATGGAGGACATGTTCAAGATGGCAGAAATTGCAGAGTGGTGTATGAGTGAAGAAGCAGTGGAGAGGCCAGAGATGAGGGAGATTGTTGTGATGCTCTCGCAGATTGCGACATCTTCGATCGAGTGGGAAGCAACACTGGGTGGCAACAGCCAGGTTTTCAGTGGTGTATTTCAATTCACTGGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LysM Pkinase Pkinase_Tyr

Protein Analysis

544

Amino Acids

60.76

Weight (kDa)

5.59

Isoelectric Point (pI)

41.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	238 - 510	1.6e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	240 - 512	3.6e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003063)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25871 FvH4_5g05950 FvH4_5g05950 FvH4_5g05950 FvH4_5g05950 FvH4_5g05950
malus_domestica	MD06G1169200.v1.1 MD13G1097500.v1.1 MD14G1174500.v1.1 MD16G1098600.v1.1
prunus_persica	Prupe.1G247900_v2.0.a1 Prupe.5G168000_v2.0.a1
pyrus_communis	pycom06g15070 pycom13g08510
rosa_chinensis	RchiOBHm_Chr4g0433311 RchiOBHm_Chr7g0192891
rosa_laevigata	RLG00000004315 RLG00000006764
rosa_multiflora	Rmu_co8450261.1_g000001 Rmu_sc0000354.1_g000008 Rmu_sc0002040.1_g000053
rosa_roxburghii	Rroxscaffold_3G00262570 Rroxscaffold_3G00262580 Rroxscaffold_5G00374870 Rroxscaffold_7G00191320
rosa_rugosa	Rorug04G0268000 Rorug07G0005400
rosa_samantha	Rh4AG322500 Rh4BG330000 Rh4CG345400 Rh4DG326700 Rh7AG131100 Rh7BG131900 Rh7CG135200 Rh7DG133000
rosa_wichuraiana	Rw0G023840 Rw4G027910 Rw7G011180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	351
AasI	GACNNNNNNGTC	1 cut(s)	665
AccI	GTMKAC	1 cut(s)	359
AccII	CGCG	1 cut(s)	1063
AciI	CCGC	3 cut(s)	375, 407, 450
AclWI	GGATC	3 cut(s)	844, 943, 953
AcsI	RAATTY	1 cut(s)	818
AcuI	CTGAAG	1 cut(s)	194
AdeI	CACNNNGTG	1 cut(s)	1583
AfaI	GTAC	5 cut(s)	200, 459, 811, 1040, 1281
AfiI	CCNNNNNNNGG	1 cut(s)	910
AflIII	ACRYGT	3 cut(s)	137, 342, 1452
AgeI	ACCGGT	1 cut(s)	701
AgsI	TTSAA	4 cut(s)	548, 557, 1459, 1616
AhlI	ACTAGT	1 cut(s)	1088
AjnI	CCWGG	4 cut(s)	909, 1239, 1333, 1593
AloI	GAACNNNNNNTCC	2 cut(s)	1439, 1471
AluBI	AGCT	6 cut(s)	832, 874, 1150, 1237, 1291, 1400
AluI	AGCT	6 cut(s)	832, 874, 1150, 1237, 1291, 1400
Alw21I	GWGCWC	2 cut(s)	1074, 1239
Alw26I	GTCTC	1 cut(s)	474
Alw44I	GTGCAC	1 cut(s)	1070
AlwI	GGATC	3 cut(s)	844, 943, 953
AlwNI	CAGNNNCTG	1 cut(s)	425
AoxI	GGCC	1 cut(s)	1508
ApaLI	GTGCAC	1 cut(s)	1070
ApeKI	GCWGC	5 cut(s)	74, 379, 1022, 1397, 1409
ApoI	RAATTY	1 cut(s)	818
AsiGI	ACCGGT	1 cut(s)	701
Asp700I	GAANNNNTTC	1 cut(s)	951
AspLEI	GCGC	2 cut(s)	1063, 1312
AsuC2I	CCSGG	1 cut(s)	1206
AsuHPI	GGTGA	3 cut(s)	340, 493, 1255
BaeGI	GKGCMC	1 cut(s)	1074
BaeI	ACNNNNGTAYC	2 cut(s)	395, 428
BanII	GRGCYC	1 cut(s)	1239
BarI	GAAGNNNNNNTAC	4 cut(s)	236, 268, 387, 419
Bbv12I	GWGCWC	2 cut(s)	1074, 1239
BbvI	GCAGC	5 cut(s)	61, 391, 1009, 1396, 1409
BccI	CCATC	8 cut(s)	21, 95, 220, 443, 447, 861, 1412, 1456
BceAI	ACGGC	1 cut(s)	1205
BcgI	CGANNNNNNTGC	2 cut(s)	1247, 1281
BciT130I	CCWGG	4 cut(s)	911, 1241, 1335, 1595
BciVI	GTATCC	1 cut(s)	296
BclI	TGATCA	2 cut(s)	1293, 1384
BcnI	CCSGG	1 cut(s)	1206
BcoDI	GTCTC	1 cut(s)	474
BcuI	ACTAGT	1 cut(s)	1088
BfaI	CTAG	2 cut(s)	1089, 1283
BfmI	CTRYAG	1 cut(s)	63
BfuI	GTATCC	1 cut(s)	296
BisI	GCNGC	5 cut(s)	75, 380, 1023, 1398, 1410
BlpI	GCTNAGC	1 cut(s)	941
BlsI	GCNGC	5 cut(s)	76, 381, 1024, 1399, 1411
Bme1390I	CCNGG	5 cut(s)	911, 1206, 1241, 1335, 1595
BmrFI	CCNGG	5 cut(s)	911, 1206, 1241, 1335, 1595
BmrI	ACTGGG	1 cut(s)	1589
BmsI	GCATC	4 cut(s)	14, 70, 92, 1524
BmuI	ACTGGG	1 cut(s)	1589
BpmI	CTGGAG	1 cut(s)	1223
Bpu1102I	GCTNAGC	1 cut(s)	941
BpuEI	CTTGAG	1 cut(s)	701
BpuMI	CCSGG	1 cut(s)	1206
Bsa29I	ATCGAT	1 cut(s)	168
BsaBI	GATNNNNATC	1 cut(s)	1386
BsaJI	CCNNGG	3 cut(s)	624, 757, 1334
BsaWI	WCCGGW	1 cut(s)	701
BsaXI	ACNNNNNCTCC	2 cut(s)	1439, 1469
Bsc4I	CCNNNNNNNGG	1 cut(s)	910
Bse118I	RCCGGY	1 cut(s)	701
Bse1I	ACTGG	5 cut(s)	466, 669, 888, 1584, 1627
Bse8I	GATNNNNATC	1 cut(s)	1386
BseBI	CCWGG	4 cut(s)	911, 1241, 1335, 1595
BseCI	ATCGAT	1 cut(s)	168
BseDI	CCNNGG	3 cut(s)	624, 757, 1334
BseGI	GGATG	5 cut(s)	5, 83, 212, 435, 1387
BseJI	GATNNNNATC	1 cut(s)	1386
BseLI	CCNNNNNNNGG	1 cut(s)	910
BseMII	CTCAG	4 cut(s)	485, 655, 932, 1441
BseNI	ACTGG	5 cut(s)	466, 669, 888, 1584, 1627
BseRI	GAGGAG	4 cut(s)	8, 26, 698, 707
BseSI	GKGCMC	1 cut(s)	1074
BseXI	GCAGC	5 cut(s)	61, 391, 1009, 1396, 1409
Bsh1236I	CGCG	1 cut(s)	1063
Bsh1285I	CGRYCG	1 cut(s)	1563
BshFI	GGCC	1 cut(s)	1510
BshTI	ACCGGT	1 cut(s)	701
BshVI	ATCGAT	1 cut(s)	168
BsiEI	CGRYCG	1 cut(s)	1563
BsiHKAI	GWGCWC	2 cut(s)	1074, 1239
BsiSI	CCGG	3 cut(s)	291, 702, 1205
BslFI	GGGAC	1 cut(s)	605
BslI	CCNNNNNNNGG	1 cut(s)	910
BsmAI	GTCTC	1 cut(s)	474
BsmFI	GGGAC	1 cut(s)	605
BsmI	GAATGC	1 cut(s)	1268
BsnI	GGCC	1 cut(s)	1510
Bsp1286I	GDGCHC	2 cut(s)	1074, 1239
Bsp1407I	TGTACA	1 cut(s)	198
Bsp143I	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
Bsp1720I	GCTNAGC	1 cut(s)	941
Bsp19I	CCATGG	1 cut(s)	624
BspACI	CCGC	3 cut(s)	375, 407, 450
BspANI	GGCC	1 cut(s)	1510
BspCNI	CTCAG	4 cut(s)	484, 654, 933, 1440
BspDI	ATCGAT	1 cut(s)	168
BspFNI	CGCG	1 cut(s)	1063
BspHI	TCATGA	3 cut(s)	132, 955, 1045
BspMAI	CTGCAG	1 cut(s)	67
BspPI	GGATC	3 cut(s)	844, 943, 953
BsrFI	RCCGGY	1 cut(s)	701
BsrGI	TGTACA	1 cut(s)	198
BsrI	ACTGG	5 cut(s)	466, 669, 888, 1584, 1627
BssAI	RCCGGY	1 cut(s)	701
BssECI	CCNNGG	3 cut(s)	624, 757, 1334
BssMI	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
BssT1I	CCWWGG	1 cut(s)	624
Bst2UI	CCWGG	4 cut(s)	911, 1241, 1335, 1595
Bst4CI	ACNGT	7 cut(s)	205, 298, 493, 513, 534, 1076, 1360
Bst6I	CTCTTC	1 cut(s)	393
BstAPI	GCANNNNNTGC	1 cut(s)	1472
BstAUI	TGTACA	1 cut(s)	198
BstC8I	GCNNGC	3 cut(s)	32, 377, 527
BstDEI	CTNAG	6 cut(s)	50, 471, 641, 720, 941, 1427
BstDSI	CCRYGG	1 cut(s)	624
BstF5I	GGATG	5 cut(s)	5, 83, 212, 435, 1387
BstFNI	CGCG	1 cut(s)	1063
BstHHI	GCGC	2 cut(s)	1063, 1312
BstKTI	GATC	7 cut(s)	172, 852, 938, 961, 1296, 1387, 1563
BstMAI	GTCTC	1 cut(s)	474
BstMBI	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
BstMCI	CGRYCG	1 cut(s)	1563
BstMWI	GCNNNNNNNGC	9 cut(s)	71, 80, 462, 1019, 1069, 1127, 1309, 1406, 1472
BstNI	CCWGG	4 cut(s)	911, 1241, 1335, 1595
BstNSI	RCATGY	3 cut(s)	34, 141, 1456
BstSCI	CCNGG	5 cut(s)	909, 1204, 1239, 1333, 1593
BstSFI	CTRYAG	1 cut(s)	63
BstSLI	GKGCMC	1 cut(s)	1074
BstUI	CGCG	1 cut(s)	1063
BstV1I	GCAGC	5 cut(s)	61, 391, 1009, 1396, 1409
BstX2I	RGATCY	1 cut(s)	849
BstXI	CCANNNNNNTGG	1 cut(s)	990
BstYI	RGATCY	1 cut(s)	849
Bsu15I	ATCGAT	1 cut(s)	168
BsuI	GTATCC	1 cut(s)	296
BsuRI	GGCC	1 cut(s)	1510
BsuTUI	ATCGAT	1 cut(s)	168
BtgI	CCRYGG	1 cut(s)	624
BtsCI	GGATG	5 cut(s)	5, 83, 212, 435, 1387
BtsI	GCAGTG	2 cut(s)	1020, 1506
BtsIMutI	CAGTG	8 cut(s)	294, 509, 895, 1020, 1506, 1577, 1609, 1620
Cac8I	GCNNGC	3 cut(s)	32, 377, 527
CaiI	CAGNNNCTG	1 cut(s)	425
CciI	TCATGA	3 cut(s)	132, 955, 1045
CfoI	GCGC	2 cut(s)	1063, 1312
Cfr10I	RCCGGY	1 cut(s)	701
ClaI	ATCGAT	1 cut(s)	168
CseI	GACGC	2 cut(s)	431, 1028
CsiI	ACCWGGT	1 cut(s)	909
Csp6I	GTAC	5 cut(s)	199, 458, 810, 1039, 1280
CspAI	ACCGGT	1 cut(s)	701
CviQI	GTAC	5 cut(s)	199, 458, 810, 1039, 1280
DdeI	CTNAG	6 cut(s)	50, 471, 641, 720, 941, 1427
DpnI	GATC	7 cut(s)	171, 851, 937, 960, 1295, 1386, 1562
DpnII	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
DraI	TTTAAA	1 cut(s)	415
DraIII	CACNNNGTG	1 cut(s)	1583
DrdI	GACNNNNNNGTC	1 cut(s)	665
DseDI	GACNNNNNNGTC	1 cut(s)	665
Eam1104I	CTCTTC	1 cut(s)	393
EarI	CTCTTC	1 cut(s)	393
Ecl136II	GAGCTC	1 cut(s)	1237
Eco130I	CCWWGG	1 cut(s)	624
Eco24I	GRGCYC	1 cut(s)	1239
Eco53kI	GAGCTC	1 cut(s)	1237
Eco57I	CTGAAG	1 cut(s)	194
EcoICRI	GAGCTC	1 cut(s)	1237
EcoRII	CCWGG	4 cut(s)	909, 1239, 1333, 1593
EcoT14I	CCWWGG	1 cut(s)	624
EcoT22I	ATGCAT	2 cut(s)	32, 85
EcoT38I	GRGCYC	1 cut(s)	1239
ErhI	CCWWGG	1 cut(s)	624
FalI	AAGNNNNNCTT	2 cut(s)	253, 285
FaqI	GGGAC	1 cut(s)	605
FauI	CCCGC	1 cut(s)	368
FbaI	TGATCA	2 cut(s)	1293, 1384
FblI	GTMKAC	1 cut(s)	359
Fnu4HI	GCNGC	5 cut(s)	75, 380, 1023, 1398, 1410
FokI	GGATG	4 cut(s)	70, 199, 422, 1374
FriOI	GRGCYC	1 cut(s)	1239
Fsp4HI	GCNGC	5 cut(s)	75, 380, 1023, 1398, 1410
FspBI	CTAG	2 cut(s)	1089, 1283
GlaI	GCGC	2 cut(s)	1062, 1311
GluI	GCNGC	5 cut(s)	75, 380, 1023, 1398, 1410
GsuI	CTGGAG	1 cut(s)	1223
HaeIII	GGCC	1 cut(s)	1510
HapII	CCGG	3 cut(s)	291, 702, 1205
HgaI	GACGC	2 cut(s)	431, 1028
HhaI	GCGC	2 cut(s)	1063, 1312
Hin6I	GCGC	2 cut(s)	1061, 1310
HinP1I	GCGC	2 cut(s)	1061, 1310
HincII	GTYRAC	1 cut(s)	360
HindII	GTYRAC	1 cut(s)	360
HindIII	AAGCTT	1 cut(s)	1148
HinfI	GANTC	5 cut(s)	208, 583, 639, 1223, 1246
HpaII	CCGG	3 cut(s)	291, 702, 1205
HphI	GGTGA	3 cut(s)	340, 493, 1255
Hpy166II	GTNNAC	5 cut(s)	106, 201, 360, 1018, 1072
Hpy188III	TCNNGA	8 cut(s)	133, 497, 928, 956, 1046, 1328, 1391, 1459
Hpy8I	GTNNAC	5 cut(s)	106, 201, 360, 1018, 1072
Hpy99I	CGWCG	1 cut(s)	364
HpyAV	CCTTC	5 cut(s)	32, 169, 597, 769, 1225
HpyCH4III	ACNGT	7 cut(s)	205, 298, 493, 513, 534, 1076, 1360
HpyCH4IV	ACGT	3 cut(s)	344, 864, 1306
HpyF10VI	GCNNNNNNNGC	9 cut(s)	71, 80, 462, 1019, 1069, 1127, 1309, 1406, 1472
HpyF3I	CTNAG	6 cut(s)	50, 471, 641, 720, 941, 1427
HpySE526I	ACGT	3 cut(s)	344, 864, 1306
HspAI	GCGC	2 cut(s)	1061, 1310
Ksp22I	TGATCA	2 cut(s)	1293, 1384
Kzo9I	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
LmnI	GCTCC	5 cut(s)	39, 499, 871, 1234, 1242
Lsp1109I	GCAGC	5 cut(s)	61, 391, 1009, 1396, 1409
LweI	GCATC	4 cut(s)	14, 70, 92, 1524
MabI	ACCWGGT	1 cut(s)	909
MaeI	CTAG	2 cut(s)	1089, 1283
MaeII	ACGT	3 cut(s)	344, 864, 1306
MaeIII	GTNAC	6 cut(s)	140, 481, 704, 1091, 1243, 1319
MalI	GATC	7 cut(s)	171, 851, 937, 960, 1295, 1386, 1562
MboI	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
MflI	RGATCY	1 cut(s)	849
MhlI	GDGCHC	2 cut(s)	1074, 1239
MluCI	AATT	7 cut(s)	186, 335, 818, 1008, 1353, 1470, 1616
MlyI	GAGTC	2 cut(s)	633, 1240
MmeI	TCCRAC	4 cut(s)	280, 495, 1135, 1177
Mph1103I	ATGCAT	2 cut(s)	32, 85
MroXI	GAANNNNTTC	1 cut(s)	951
MseI	TTAA	4 cut(s)	414, 861, 969, 1352
MslI	CAYNNNNRTG	3 cut(s)	599, 863, 954
MspA1I	CMGCKG	1 cut(s)	375
MspI	CCGG	3 cut(s)	291, 702, 1205
MspR9I	CCNGG	5 cut(s)	911, 1206, 1241, 1335, 1595
Mva1269I	GAATGC	1 cut(s)	1268
MvaI	CCWGG	4 cut(s)	911, 1241, 1335, 1595
MvnI	CGCG	1 cut(s)	1063
MwoI	GCNNNNNNNGC	9 cut(s)	71, 80, 462, 1019, 1069, 1127, 1309, 1406, 1472
NciI	CCSGG	1 cut(s)	1206
NcoI	CCATGG	1 cut(s)	624
NdeII	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
NmuCI	GTSAC	3 cut(s)	481, 1243, 1319
NsiI	ATGCAT	2 cut(s)	32, 85
NspI	RCATGY	3 cut(s)	34, 141, 1456
PaeI	GCATGC	1 cut(s)	34
PagI	TCATGA	3 cut(s)	132, 955, 1045
PciI	ACATGT	2 cut(s)	137, 1452
PcsI	WCGNNNNNNNCGW	1 cut(s)	1547
PctI	GAATGC	1 cut(s)	1268
PdmI	GAANNNNTTC	1 cut(s)	951
PfeI	GAWTC	3 cut(s)	208, 583, 1223
PinAI	ACCGGT	1 cut(s)	701
PkrI	GCNGC	5 cut(s)	76, 381, 1024, 1399, 1411
Ple19I	CGATCG	1 cut(s)	1563
PleI	GAGTC	2 cut(s)	633, 1240
PpsI	GAGTC	2 cut(s)	633, 1240
PscI	ACATGT	2 cut(s)	137, 1452
PsiI	TTATAA	1 cut(s)	351
Psp124BI	GAGCTC	1 cut(s)	1239
Psp6I	CCWGG	4 cut(s)	909, 1239, 1333, 1593
PspGI	CCWGG	4 cut(s)	909, 1239, 1333, 1593
PstI	CTGCAG	1 cut(s)	67
PstNI	CAGNNNCTG	1 cut(s)	425
PsuI	RGATCY	1 cut(s)	849
PvuI	CGATCG	1 cut(s)	1563
RsaI	GTAC	5 cut(s)	200, 459, 811, 1040, 1281
RsaNI	GTAC	5 cut(s)	199, 458, 810, 1039, 1280
RseI	CAYNNNNRTG	3 cut(s)	599, 863, 954
SacI	GAGCTC	1 cut(s)	1239
SalI	GTCGAC	1 cut(s)	358
SaqAI	TTAA	4 cut(s)	414, 861, 969, 1352
SatI	GCNGC	5 cut(s)	75, 380, 1023, 1398, 1410
Sau3AI	GATC	7 cut(s)	169, 849, 935, 958, 1293, 1384, 1560
SchI	GAGTC	2 cut(s)	633, 1240
ScrFI	CCNGG	5 cut(s)	911, 1206, 1241, 1335, 1595
SduI	GDGCHC	2 cut(s)	1074, 1239
SexAI	ACCWGGT	1 cut(s)	909
SfaNI	GCATC	4 cut(s)	14, 70, 92, 1524
SfcI	CTRYAG	1 cut(s)	63
SmiMI	CAYNNNNRTG	3 cut(s)	599, 863, 954
SmlI	CTYRAG	1 cut(s)	680
SmoI	CTYRAG	1 cut(s)	680
SpeI	ACTAGT	1 cut(s)	1088
SphI	GCATGC	1 cut(s)	34
Sse9I	AATT	7 cut(s)	186, 335, 818, 1008, 1353, 1470, 1616
SsiI	CCGC	3 cut(s)	375, 407, 450
SspMI	CTAG	2 cut(s)	1089, 1283
SstI	GAGCTC	1 cut(s)	1239
StyD4I	CCNGG	5 cut(s)	909, 1204, 1239, 1333, 1593
StyI	CCWWGG	1 cut(s)	624
TaaI	ACNGT	7 cut(s)	205, 298, 493, 513, 534, 1076, 1360
TaiI	ACGT	3 cut(s)	347, 867, 1309
TaqI	TCGA	7 cut(s)	168, 359, 393, 711, 1327, 1559, 1563
TaqII	GACCGA	1 cut(s)	593
TasI	AATT	7 cut(s)	186, 335, 818, 1008, 1353, 1470, 1616
TatI	WGTACW	2 cut(s)	198, 1038
TfiI	GAWTC	3 cut(s)	208, 583, 1223
Tru1I	TTAA	4 cut(s)	414, 861, 969, 1352
Tru9I	TTAA	4 cut(s)	414, 861, 969, 1352
TscAI	CASTG	8 cut(s)	301, 516, 895, 1020, 1506, 1584, 1609, 1627
TseFI	GTSAC	3 cut(s)	481, 1243, 1319
TseI	GCWGC	5 cut(s)	74, 379, 1022, 1397, 1409
Tsp45I	GTSAC	3 cut(s)	481, 1243, 1319
TspDTI	ATGAA	8 cut(s)	578, 579, 729, 812, 944, 1034, 1182, 1358
TspGWI	ACGGA	3 cut(s)	675, 1216, 1307
TspRI	CASTG	8 cut(s)	301, 516, 895, 1020, 1506, 1584, 1609, 1627
VneI	GTGCAC	1 cut(s)	1070
XapI	RAATTY	1 cut(s)	818
XceI	RCATGY	3 cut(s)	34, 141, 1456
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1601
XmiI	GTMKAC	1 cut(s)	359
XmnI	GAANNNNTTC	1 cut(s)	951
XspI	CTAG	2 cut(s)	1089, 1283
Zsp2I	ATGCAT	2 cut(s)	32, 85

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.