Rw7G011180

lysM domain receptor-like kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003063
Physical Location & Seq
Forward (+)
10454468 .. 10457739
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011180.1

Sequence Viewer

Length: 1734 bp
ATGGCTTCCCTGAACTTTCTCTTCATTCTCTTCACCATTTTGTACGCTTCCGTCCTCTCTGCCAGTGATAGTGTTGGCGCAGAGCCAATTCACATATACCCTTTTACTTGCTCTGCTTCTCATCACATCCCGAGCTGCAATTCCAAGCTGTATCACATTTCTACAGGCCATCAAATTGAAGAAATAGCCTATTTTTACAAGGTGAATGTATCCGAGCTTGAGCCCATAAACCGTGATCAAAGTCAAAAGGACTATCTTGTATCTGTGCCTTGCAGTTGTAAGGACATTGATGGCACACAAATGTACGTGTATGATACCAATTATCCAGTTCAATCAGGGGACACATTTGAGAGAGTTGCAGGCGAGTTTTACAGTAGCCAAGCCTTGAGGGGGGAAGGCAATTTGAGCACCATACATCTTGTTTGTGGCTGCGTGAAGAAAAAGTCGCAGGAGATTGTGACATACACAGTTCAAGAGCATGACACTTTATCAGGAATTGGACATCTTCTAGATGCATACGAGTCTGAAATTGAGACATACAATAGAAACTTTACTAAAGATCCGAGTTTTCTAGATGTAGGTTGGGTGTTGTATGTGCCAAAGAATGGACTTCGAGCTAACAAGAAAGGAAAAAGACTCAAGGTGTCTATAGTCATAGGTGTATCGTCCGCTGTTTGTTTTGTTTTGGTGGCCGCCTTCATTGTGTTCCTTCTAATGAGAAATAGGATCTGTAGGAACCTAGGGGATGATTTGCACCTTGTAAACTCAAGTGCGAAGAAACGTTCCTCGCTGAAGCAACAGTTTTTTAAAAAGGAAATGGAAGAAACTTTTGAGAATGATAGACCTGTTATATATACTATGGAGGAGATCAAAGAGGCTACAAATAACTTTGACGATACCAGAAAGATTGGGGAGGGTGGATACGGTTGTGTTTACTTTGCCATAATAGGAGAAGTAAAAGTTGCAATTAAGAATATGAGATCTACCAGAACAAAAGAATTCTTTGCTGAGCTCAAGGTGTTATGCAAGATACATCATAACAATGTGGTGGAGCTGCTAGGATATGCTAGTGGAAGTGAACATCTTTGTTTGGTATATGAGTTTGTTCAGAATGGATCTCTCAACGACCATCTTCATGATCCATTGTTGAAAGGTCGCCAACCTCTTTCATGGACTGCAAGAGCACAGATAGCACTGGATACTGCAAGAGGGATTGAATACATTCATGATCACACGAAGAAACGCTATGTCCACCGTGATATAAAAACGAGTAATATTCTACTTGATGAAGGACTCAGAGCCAAGGTAGCAGATTTTGGGCTAGCAAGGTTAGTGGAACGGTCTAATGAAGAAGATATAGTTGCAACTCGTGTAGTTGGAACACCCGGTTACATTCCTCCTGAATCGGTGCGGGAGCTACAAATGACAGCAAAAGGTGATGTATATGCATTCGGAGTGGTTATAGGAGAGCTAATAACTGGTCAGCGTGCCATTGTTCGTGACAACCGAGAGCCAAAAAGGATGAAATCACTGGTCTCAGTTTTGTTAGAAGTATTCCAAGATAATGATCCAGAGATTGCTTTGGAAGCTAAAATAGATGGTAATTTGAAAGGAAGCTACCCCATGGAAGAAGTCTACAAGAGATTTTATTTTGTTTTTACCCTTAGATTGGTTGATAAAAATGAGCGACAACAAGTGACGATGATCTCGAAACACTGTATTGGGCTTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LysM Pkinase

Protein Analysis

577

Amino Acids

65.27

Weight (kDa)

6.67

Isoelectric Point (pI)

36.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 298 - 511 1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 300 - 496 5e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 605
AccI GTMKAC 1 cut(s) 1635
AciI CCGC 3 cut(s) 669, 693, 1411
AclI AACGTT 1 cut(s) 781
AclWI GGATC 5 cut(s) 554, 734, 1123, 1133, 1562
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 1 cut(s) 998
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 2 cut(s) 44, 305
AfiI CCNNNNNNNGG 3 cut(s) 390, 605, 1669
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 6 cut(s) 179, 332, 473, 1150, 1217, 1609
Alw21I GWGCWC 3 cut(s) 410, 1014, 1186
Alw26I GTCTC 2 cut(s) 527, 1540
AlwI GGATC 5 cut(s) 554, 734, 1123, 1133, 1562
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 2 cut(s) 166, 690
ApeKI GCWGC 3 cut(s) 135, 429, 1054
ApoI RAATTY 1 cut(s) 998
Asp700I GAANNNNTTC 1 cut(s) 1221
AspA2I CCTAGG 1 cut(s) 739
AspLEI GCGC 1 cut(s) 80
AsuC2I CCSGG 1 cut(s) 1386
AsuHPI GGTGA 3 cut(s) 25, 214, 1448
AsuNHI GCTAGC 1 cut(s) 1321
AvaI CYCGRG 1 cut(s) 130
AvrII CCTAGG 1 cut(s) 739
BaeI ACNNNNGTAYC 2 cut(s) 913, 946
BanII GRGCYC 2 cut(s) 225, 1014
BauI CACGAG 1 cut(s) 1368
Bbv12I GWGCWC 3 cut(s) 410, 1014, 1186
BbvI GCAGC 3 cut(s) 122, 416, 1041
BccI CCATC 4 cut(s) 177, 284, 1137, 1592
BciVI GTATCC 3 cut(s) 220, 914, 1192
BclI TGATCA 2 cut(s) 235, 1228
BcnI CCSGG 1 cut(s) 1386
BcoDI GTCTC 2 cut(s) 527, 1540
BfaI CTAG 6 cut(s) 509, 572, 740, 1058, 1068, 1322
BfmI CTRYAG 3 cut(s) 162, 648, 730
BfuI GTATCC 3 cut(s) 220, 914, 1192
BglII AGATCT 1 cut(s) 980
BisI GCNGC 4 cut(s) 136, 430, 693, 1055
BlnI CCTAGG 1 cut(s) 739
BlpI GCTNAGC 1 cut(s) 1008
BlsI GCNGC 4 cut(s) 137, 431, 694, 1056
Bme1390I CCNGG 1 cut(s) 1386
BmeT110I CYCGRG 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 737
BmrFI CCNGG 1 cut(s) 1386
BmsI GCATC 1 cut(s) 502
BmtI GCTAGC 1 cut(s) 1325
Bpu1102I GCTNAGC 1 cut(s) 1008
BpuEI CTTGAG 5 cut(s) 239, 406, 623, 751, 998
BpuMI CCSGG 1 cut(s) 1386
BsaAI YACGTR 1 cut(s) 307
BsaBI GATNNNNATC 1 cut(s) 1566
BsaI GGTCTC 1 cut(s) 1540
BsaJI CCNNGG 3 cut(s) 739, 1302, 1623
Bsc4I CCNNNNNNNGG 3 cut(s) 390, 605, 1669
Bse1I ACTGG 5 cut(s) 63, 326, 1200, 1483, 1536
Bse8I GATNNNNATC 1 cut(s) 1566
BseDI CCNNGG 3 cut(s) 739, 1302, 1623
BseGI GGATG 3 cut(s) 126, 751, 1527
BseJI GATNNNNATC 1 cut(s) 1566
BseLI CCNNNNNNNGG 3 cut(s) 390, 605, 1669
BseMII CTCAG 3 cut(s) 999, 1309, 1551
BseNI ACTGG 5 cut(s) 63, 326, 1200, 1483, 1536
BseRI GAGGAG 1 cut(s) 878
BseXI GCAGC 3 cut(s) 122, 416, 1041
BshFI GGCC 2 cut(s) 168, 692
BsiHKAI GWGCWC 3 cut(s) 410, 1014, 1186
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 1 cut(s) 1386
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 3 cut(s) 390, 605, 1669
BsmAI GTCTC 2 cut(s) 527, 1540
BsmFI GGGAC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 1448
BsnI GGCC 2 cut(s) 168, 692
Bso31I GGTCTC 1 cut(s) 1540
BsoBI CYCGRG 1 cut(s) 130
Bsp1286I GDGCHC 4 cut(s) 225, 410, 1014, 1186
Bsp1720I GCTNAGC 1 cut(s) 1008
Bsp19I CCATGG 1 cut(s) 1623
BspACI CCGC 3 cut(s) 669, 693, 1411
BspANI GGCC 2 cut(s) 168, 692
BspCNI CTCAG 3 cut(s) 1000, 1308, 1550
BspHI TCATGA 2 cut(s) 1135, 1225
BspLI GGNNCC 1 cut(s) 737
BspOI GCTAGC 1 cut(s) 1325
BspPI GGATC 5 cut(s) 554, 734, 1123, 1133, 1562
BspTNI GGTCTC 1 cut(s) 1540
BsrI ACTGG 5 cut(s) 63, 326, 1200, 1483, 1536
BssECI CCNNGG 3 cut(s) 739, 1302, 1623
BssSI CACGAG 1 cut(s) 1368
BssT1I CCWWGG 3 cut(s) 739, 1302, 1623
Bst2BI CACGAG 1 cut(s) 1368
Bst4CI ACNGT 8 cut(s) 233, 374, 469, 801, 926, 1256, 1341, 1718
Bst6I CTCTTC 2 cut(s) 26, 35
BstBAI YACGTR 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 361, 1323, 1488
BstDEI CTNAG 4 cut(s) 1008, 1295, 1537, 1664
BstDSI CCRYGG 1 cut(s) 1623
BstF5I GGATG 3 cut(s) 126, 751, 1527
BstHHI GCGC 1 cut(s) 80
BstMAI GTCTC 2 cut(s) 527, 1540
BstMWI GCNNNNNNNGC 4 cut(s) 405, 1190, 1307, 1586
BstSCI CCNGG 1 cut(s) 1384
BstSFI CTRYAG 3 cut(s) 162, 648, 730
BstV1I GCAGC 3 cut(s) 122, 416, 1041
BstX2I RGATCY 4 cut(s) 559, 726, 980, 1115
BstYI RGATCY 4 cut(s) 559, 726, 980, 1115
BsuI GTATCC 3 cut(s) 220, 914, 1192
BsuRI GGCC 2 cut(s) 168, 692
BtgI CCRYGG 1 cut(s) 1623
BtsCI GGATG 3 cut(s) 126, 751, 1527
BtsIMutI CAGTG 4 cut(s) 70, 1193, 1529, 1714
Cac8I GCNNGC 3 cut(s) 361, 1323, 1488
CciI TCATGA 2 cut(s) 1135, 1225
CfoI GCGC 1 cut(s) 80
Csp6I GTAC 2 cut(s) 43, 304
CspCI CAANNNNNGTGG 2 cut(s) 1314, 1349
CviAII CATG 5 cut(s) 479, 1136, 1170, 1226, 1624
CviQI GTAC 2 cut(s) 43, 304
DdeI CTNAG 4 cut(s) 1008, 1295, 1537, 1664
DraI TTTAAA 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 2 cut(s) 26, 35
EarI CTCTTC 2 cut(s) 26, 35
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 3 cut(s) 739, 1302, 1623
Eco24I GRGCYC 2 cut(s) 225, 1014
Eco31I GGTCTC 1 cut(s) 1540
Eco53kI GAGCTC 1 cut(s) 1012
Eco57I CTGAAG 1 cut(s) 812
Eco88I CYCGRG 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 1012
EcoRI GAATTC 1 cut(s) 998
EcoT14I CCWWGG 3 cut(s) 739, 1302, 1623
EcoT22I ATGCAT 2 cut(s) 517, 1450
EcoT38I GRGCYC 2 cut(s) 225, 1014
ErhI CCWWGG 3 cut(s) 739, 1302, 1623
FaeI CATG 5 cut(s) 482, 1139, 1173, 1229, 1627
FaqI GGGAC 1 cut(s) 353
FatI CATG 5 cut(s) 478, 1135, 1169, 1225, 1623
FauI CCCGC 1 cut(s) 1404
FbaI TGATCA 2 cut(s) 235, 1228
FblI GTMKAC 1 cut(s) 1635
Fnu4HI GCNGC 4 cut(s) 136, 430, 693, 1055
FokI GGATG 3 cut(s) 113, 758, 1534
FriOI GRGCYC 2 cut(s) 225, 1014
Fsp4HI GCNGC 4 cut(s) 136, 430, 693, 1055
FspBI CTAG 6 cut(s) 509, 572, 740, 1058, 1068, 1322
GlaI GCGC 1 cut(s) 79
GluI GCNGC 4 cut(s) 136, 430, 693, 1055
HaeIII GGCC 2 cut(s) 168, 692
HapII CCGG 1 cut(s) 1386
HhaI GCGC 1 cut(s) 80
Hin1II CATG 5 cut(s) 482, 1139, 1173, 1229, 1627
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HinfI GANTC 4 cut(s) 521, 636, 1293, 1403
HpaII CCGG 1 cut(s) 1386
HphI GGTGA 3 cut(s) 25, 214, 1448
Hpy166II GTNNAC 5 cut(s) 763, 934, 1079, 1252, 1636
Hpy188I TCNGA 6 cut(s) 214, 526, 564, 1110, 1298, 1454
Hpy8I GTNNAC 5 cut(s) 763, 934, 1079, 1252, 1636
HpyAV CCTTC 4 cut(s) 389, 706, 719, 1283
HpyCH4III ACNGT 8 cut(s) 233, 374, 469, 801, 926, 1256, 1341, 1718
HpyCH4IV ACGT 2 cut(s) 306, 781
HpyF10VI GCNNNNNNNGC 4 cut(s) 405, 1190, 1307, 1586
HpyF3I CTNAG 4 cut(s) 1008, 1295, 1537, 1664
HpySE526I ACGT 2 cut(s) 306, 781
Hsp92II CATG 5 cut(s) 482, 1139, 1173, 1229, 1627
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 2 cut(s) 235, 1228
LmnI GCTCC 2 cut(s) 1051, 1414
Lsp1109I GCAGC 3 cut(s) 122, 416, 1041
LweI GCATC 1 cut(s) 502
MaeI CTAG 6 cut(s) 509, 572, 740, 1058, 1068, 1322
MaeII ACGT 2 cut(s) 306, 781
MaeIII GTNAC 4 cut(s) 457, 1388, 1499, 1696
MflI RGATCY 4 cut(s) 559, 726, 980, 1115
MhlI GDGCHC 4 cut(s) 225, 410, 1014, 1186
MlyI GAGTC 3 cut(s) 530, 630, 1287
MmeI TCCRAC 1 cut(s) 1357
MnlI CCTC 9 cut(s) 65, 381, 796, 856, 868, 907, 1173, 1202, 1407
Mph1103I ATGCAT 2 cut(s) 517, 1450
MroXI GAANNNNTTC 1 cut(s) 1221
MseI TTAA 2 cut(s) 807, 969
MslI CAYNNNNRTG 3 cut(s) 299, 1041, 1134
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 1 cut(s) 1386
MspR9I CCNGG 1 cut(s) 1386
Mva1269I GAATGC 1 cut(s) 1448
MwoI GCNNNNNNNGC 4 cut(s) 405, 1190, 1307, 1586
NciI CCSGG 1 cut(s) 1386
NcoI CCATGG 1 cut(s) 1623
NheI GCTAGC 1 cut(s) 1321
NlaIII CATG 5 cut(s) 482, 1139, 1173, 1229, 1627
NlaIV GGNNCC 1 cut(s) 737
NmuCI GTSAC 3 cut(s) 457, 1499, 1696
NsiI ATGCAT 2 cut(s) 517, 1450
PagI TCATGA 2 cut(s) 1135, 1225
PcsI WCGNNNNNNNCGW 2 cut(s) 1504, 1706
PctI GAATGC 1 cut(s) 1448
PdmI GAANNNNTTC 1 cut(s) 1221
PfeI GAWTC 1 cut(s) 1403
PflMI CCANNNNNTGG 1 cut(s) 605
PkrI GCNGC 4 cut(s) 137, 431, 694, 1056
PleI GAGTC 3 cut(s) 529, 630, 1287
PpsI GAGTC 3 cut(s) 529, 630, 1287
Ppu21I YACGTR 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 1014
Psp1406I AACGTT 1 cut(s) 781
PspN4I GGNNCC 1 cut(s) 737
PsuI RGATCY 4 cut(s) 559, 726, 980, 1115
RsaI GTAC 2 cut(s) 44, 305
RsaNI GTAC 2 cut(s) 43, 304
RseI CAYNNNNRTG 3 cut(s) 299, 1041, 1134
SacI GAGCTC 1 cut(s) 1014
SaqAI TTAA 2 cut(s) 807, 969
SatI GCNGC 4 cut(s) 136, 430, 693, 1055
SchI GAGTC 3 cut(s) 530, 630, 1287
ScrFI CCNGG 1 cut(s) 1386
SduI GDGCHC 4 cut(s) 225, 410, 1014, 1186
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 3 cut(s) 162, 648, 730
SmiMI CAYNNNNRTG 3 cut(s) 299, 1041, 1134
SmlI CTYRAG 5 cut(s) 218, 385, 638, 766, 1013
SmoI CTYRAG 5 cut(s) 218, 385, 638, 766, 1013
SsiI CCGC 3 cut(s) 669, 693, 1411
SspI AATATT 1 cut(s) 1276
SspMI CTAG 6 cut(s) 509, 572, 740, 1058, 1068, 1322
SstI GAGCTC 1 cut(s) 1014
StyD4I CCNGG 1 cut(s) 1384
StyI CCWWGG 3 cut(s) 739, 1302, 1623
TaaI ACNGT 8 cut(s) 233, 374, 469, 801, 926, 1256, 1341, 1718
TaiI ACGT 2 cut(s) 309, 784
TaqI TCGA 2 cut(s) 613, 1709
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 1 cut(s) 1403
Tru1I TTAA 2 cut(s) 807, 969
Tru9I TTAA 2 cut(s) 807, 969
TscAI CASTG 4 cut(s) 70, 1200, 1536, 1721
TseFI GTSAC 3 cut(s) 457, 1499, 1696
TseI GCWGC 3 cut(s) 135, 429, 1054
Tsp45I GTSAC 3 cut(s) 457, 1499, 1696
TspDTI ATGAA 8 cut(s) 13, 688, 1124, 1158, 1214, 1302, 1362, 1538
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 4 cut(s) 70, 1200, 1536, 1721
Van91I CCANNNNNTGG 1 cut(s) 605
XapI RAATTY 1 cut(s) 998
XbaI TCTAGA 2 cut(s) 508, 571
XmaJI CCTAGG 1 cut(s) 739
XmiI GTMKAC 1 cut(s) 1635
XmnI GAANNNNTTC 1 cut(s) 1221
XspI CTAG 6 cut(s) 509, 572, 740, 1058, 1068, 1322
Zsp2I ATGCAT 2 cut(s) 517, 1450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.