MD16G1149400.v1.1

Desiccation-related protein PCC13-62-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003995
Physical Location & Seq
Forward (+)
11765756 .. 11770164
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1149400.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGCCACATTTGCTTATGCCACCATCACCGCCATTGTCTTCCTCATCCTCCTCCCGCTATCTTGCTCTTCCGAATCAATTTTGCACCACCATCACCATGGCGGAAACTCCTCCGTCCAGAAATCGGACGTCGATCTTTTGGAGTTTCCTTTGAATTTAGAGTATTTGGAAGCTGAGTTCTTTTTGTATGGCTCTTTGGGGTATGGCTTGGATAAAGTTTATCCGAGCTTAGCCCAAGGAGGTCCCTCTCCCATTGGTGCTAAAAAGGCACATTTGGACCACTTTACTAGGGATGTCATCGAGCAATTTGGATGGCAAGAAGTTGGACACTTTTCTATTAAGAAAACAGTGAAGGGTTTCCCAAGGCCATTGTTGAATATAAGTGCAGCATCATTTGCTCATGTGTTTGATTCTGCATTTGGGAGTCCCTTAAATCCACCATTTGACCCTTATGCCAATTCAATCAACTATCTCCTTGCATCCTATATCATTCCTTCCCCTAAGCTCCAAGGTGCTACTTCCAAAAGGCTAGTTGCAGGTCTTTTGGGTGTGGAATCAGGCCAAGATGCAGTGATTCGAGCATTGCTATACCAGCGCGCAGAGTTGAAAGTGGAACCATATGGCATTACAGTGGCTGAGTTCACAAATCGCATTTCGGAACTAAGGAATAAGCTAGGACACAAAGGTTTGAAAGATGAAGGCCTTGTGGTTCCCAAACACTTGGGTGCTGAGGGAAAAGTTAGCGGCAACATTCTCGTTGGAGACGAGTACTCACTTGCATATGGCAGGACTCCAGAGGAGATACTAAGGATCGTATATGGTAGCGGTAATGAACATGTACCGGGCGGTTTCTACCCTAAAGGAGCTGACGGCCGGATTGCTAAATCTTATTTGAAAAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ferritin_2

Protein Analysis

302

Amino Acids

32.78

Weight (kDa)

6.64

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin_2 PF13668 42 - 198 1.9e-21 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 527
AccII CGCG 1 cut(s) 597
AciI CCGC 6 cut(s) 30, 56, 102, 744, 825, 846
AclWI GGATC 1 cut(s) 818
AcoI YGGCCR 2 cut(s) 3, 871
AcsI RAATTY 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 2 cut(s) 770, 840
AfiI CCNNNNNNNGG 1 cut(s) 124
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 6 cut(s) 154, 376, 462, 607, 691, 895
AleI CACNNNNGTG 1 cut(s) 723
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 5 cut(s) 173, 228, 505, 673, 866
AluI AGCT 5 cut(s) 173, 228, 505, 673, 866
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 1 cut(s) 818
AlwNI CAGNNNCTG 1 cut(s) 635
AoxI GGCC 5 cut(s) 3, 365, 559, 700, 871
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 154
Asp700I GAANNNNTTC 1 cut(s) 356
AspLEI GCGC 2 cut(s) 597, 599
AspS9I GGNCC 2 cut(s) 242, 277
AsuC2I CCSGG 1 cut(s) 843
AsuHPI GGTGA 2 cut(s) 19, 86
AvaII GGWCC 2 cut(s) 242, 277
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 31
BbvCI CCTCAGC 1 cut(s) 729
BbvI GCAGC 1 cut(s) 398
BccI CCATC 3 cut(s) 32, 99, 306
BceAI ACGGC 1 cut(s) 886
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BcnI CCSGG 1 cut(s) 843
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 3 cut(s) 288, 530, 674
BfuAI ACCTGC 1 cut(s) 527
BisI GCNGC 2 cut(s) 387, 745
BlpI GCTNAGC 1 cut(s) 229
BlsI GCNGC 2 cut(s) 388, 746
BmcAI AGTACT 1 cut(s) 770
Bme1390I CCNGG 1 cut(s) 843
Bme18I GGWCC 2 cut(s) 242, 277
BmgT120I GGNCC 2 cut(s) 242, 277
BmiI GGNNCC 3 cut(s) 244, 615, 711
BmrFI CCNGG 1 cut(s) 843
BmsI GCATC 3 cut(s) 398, 488, 556
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 777
Bpu10I CCTNAGC 2 cut(s) 501, 729
Bpu1102I GCTNAGC 1 cut(s) 229
BpuMI CCSGG 1 cut(s) 843
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 4 cut(s) 97, 235, 362, 508
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 581
BseDI CCNNGG 4 cut(s) 97, 235, 362, 508
BseGI GGATG 4 cut(s) 45, 298, 317, 479
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 3 cut(s) 165, 627, 720
BsePI GCGCGC 1 cut(s) 595
BseRI GAGGAG 3 cut(s) 41, 100, 812
BseX3I CGGCCG 1 cut(s) 871
BseXI GCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 597
Bsh1285I CGRYCG 1 cut(s) 874
BshFI GGCC 5 cut(s) 5, 367, 561, 702, 873
BsiEI CGRYCG 1 cut(s) 874
BsiSI CCGG 2 cut(s) 842, 874
BslFI GGGAC 2 cut(s) 228, 411
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 756
BsmBI CGTCTC 1 cut(s) 756
BsmFI GGGAC 2 cut(s) 228, 411
BsnI GGCC 5 cut(s) 5, 367, 561, 702, 873
Bsp143I GATC 2 cut(s) 133, 810
Bsp1720I GCTNAGC 1 cut(s) 229
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 6 cut(s) 30, 56, 102, 744, 825, 846
BspANI GGCC 5 cut(s) 5, 367, 561, 702, 873
BspCNI CTCAG 3 cut(s) 166, 628, 721
BspFNI CGCG 1 cut(s) 597
BspLI GGNNCC 3 cut(s) 244, 615, 711
BspMI ACCTGC 1 cut(s) 527
BspPI GGATC 1 cut(s) 818
BspQI GCTCTTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 581
BssECI CCNNGG 4 cut(s) 97, 235, 362, 508
BssHII GCGCGC 1 cut(s) 595
BssMI GATC 2 cut(s) 133, 810
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 4 cut(s) 97, 235, 362, 508
Bst4CI ACNGT 2 cut(s) 349, 631
Bst6I CTCTTC 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 7 cut(s) 174, 229, 501, 636, 662, 729, 806
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 4 cut(s) 45, 298, 317, 479
BstFNI CGCG 1 cut(s) 597
BstHHI GCGC 2 cut(s) 597, 599
BstKTI GATC 2 cut(s) 136, 813
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 2 cut(s) 133, 810
BstMCI CGRYCG 1 cut(s) 874
BstMWI GCNNNNNNNGC 4 cut(s) 11, 266, 395, 592
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 1 cut(s) 841
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 1 cut(s) 398
BstV2I GAAGAC 1 cut(s) 31
BstXI CCANNNNNNTGG 2 cut(s) 98, 721
BstZI CGGCCG 1 cut(s) 871
BsuRI GGCC 5 cut(s) 5, 367, 561, 702, 873
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 4 cut(s) 45, 298, 317, 479
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 3 cut(s) 354, 576, 636
BveI ACCTGC 1 cut(s) 527
Cac8I GCNNGC 1 cut(s) 597
CaiI CAGNNNCTG 1 cut(s) 635
CfoI GCGC 2 cut(s) 597, 599
Cfr13I GGNCC 2 cut(s) 242, 277
Csp6I GTAC 2 cut(s) 769, 839
CviAII CATG 3 cut(s) 98, 401, 836
CviQI GTAC 2 cut(s) 769, 839
DdeI CTNAG 7 cut(s) 174, 229, 501, 636, 662, 729, 806
DpnI GATC 2 cut(s) 135, 812
DpnII GATC 2 cut(s) 133, 810
EaeI YGGCCR 2 cut(s) 3, 871
EagI CGGCCG 1 cut(s) 871
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
EciI GGCGGA 1 cut(s) 117
EclXI CGGCCG 1 cut(s) 871
Eco130I CCWWGG 4 cut(s) 97, 235, 362, 508
Eco147I AGGCCT 1 cut(s) 702
Eco47I GGWCC 2 cut(s) 242, 277
Eco52I CGGCCG 1 cut(s) 871
EcoO109I RGGNCCY 1 cut(s) 242
EcoT14I CCWWGG 4 cut(s) 97, 235, 362, 508
ErhI CCWWGG 4 cut(s) 97, 235, 362, 508
Esp3I CGTCTC 1 cut(s) 756
FaeI CATG 3 cut(s) 101, 404, 839
FaqI GGGAC 2 cut(s) 228, 411
FatI CATG 3 cut(s) 97, 400, 835
FauI CCCGC 1 cut(s) 63
FauNDI CATATG 2 cut(s) 619, 781
Fnu4HI GCNGC 2 cut(s) 387, 745
FokI GGATG 4 cut(s) 32, 305, 324, 466
Fsp4HI GCNGC 2 cut(s) 387, 745
FspBI CTAG 3 cut(s) 288, 530, 674
GlaI GCGC 2 cut(s) 596, 598
GluI GCNGC 2 cut(s) 387, 745
GsuI CTGGAG 1 cut(s) 777
HaeIII GGCC 5 cut(s) 5, 367, 561, 702, 873
HapII CCGG 2 cut(s) 842, 874
HhaI GCGC 2 cut(s) 597, 599
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 3 cut(s) 101, 404, 839
Hin6I GCGC 2 cut(s) 595, 597
HinP1I GCGC 2 cut(s) 595, 597
HinfI GANTC 6 cut(s) 74, 410, 424, 554, 574, 790
HpaII CCGG 2 cut(s) 842, 874
HphI GGTGA 2 cut(s) 19, 86
Hpy166II GTNNAC 1 cut(s) 642
Hpy188I TCNGA 4 cut(s) 73, 127, 225, 658
Hpy188III TCNNGA 2 cut(s) 118, 794
Hpy8I GTNNAC 1 cut(s) 642
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 3 cut(s) 346, 504, 692
HpyCH4III ACNGT 2 cut(s) 349, 631
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 7 cut(s) 85, 386, 416, 479, 536, 569, 779
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 266, 395, 592
HpyF3I CTNAG 7 cut(s) 174, 229, 501, 636, 662, 729, 806
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 3 cut(s) 101, 404, 839
HspAI GCGC 2 cut(s) 595, 597
Kzo9I GATC 2 cut(s) 133, 810
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 2 cut(s) 510, 863
LpnPI CCDG 8 cut(s) 131, 522, 543, 605, 772, 807, 855, 887
Lsp1109I GCAGC 1 cut(s) 398
LweI GCATC 3 cut(s) 398, 488, 556
MaeI CTAG 3 cut(s) 288, 530, 674
MaeII ACGT 1 cut(s) 129
MalI GATC 2 cut(s) 135, 812
MboI GATC 2 cut(s) 133, 810
MboII GAAGA 2 cut(s) 31, 60
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 78, 154, 305, 457
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 433, 784
MmeI TCCRAC 2 cut(s) 304, 739
MnlI CCTC 8 cut(s) 53, 59, 62, 121, 233, 256, 724, 790
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 356
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 339, 431
MslI CAYNNNNRTG 3 cut(s) 96, 629, 723
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 842, 874
MspR9I CCNGG 1 cut(s) 843
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 4 cut(s) 11, 266, 395, 592
NciI CCSGG 1 cut(s) 843
NcoI CCATGG 1 cut(s) 97
NdeI CATATG 2 cut(s) 619, 781
NdeII GATC 2 cut(s) 133, 810
NlaIII CATG 3 cut(s) 101, 404, 839
NlaIV GGNNCC 3 cut(s) 244, 615, 711
NspI RCATGY 1 cut(s) 839
OliI CACNNNNGTG 1 cut(s) 723
PauI GCGCGC 1 cut(s) 595
PceI AGGCCT 1 cut(s) 702
PciI ACATGT 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 4 cut(s) 74, 410, 554, 574
PkrI GCNGC 2 cut(s) 388, 746
PleI GAGTC 2 cut(s) 432, 784
PpsI GAGTC 2 cut(s) 432, 784
PpuMI RGGWCCY 1 cut(s) 242
PscI ACATGT 1 cut(s) 835
Psp5II RGGWCCY 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 244, 615, 711
PspPI GGNCC 2 cut(s) 242, 277
PspPPI RGGWCCY 1 cut(s) 242
PstNI CAGNNNCTG 1 cut(s) 635
PteI GCGCGC 1 cut(s) 595
RsaI GTAC 2 cut(s) 770, 840
RsaNI GTAC 2 cut(s) 769, 839
RseI CAYNNNNRTG 3 cut(s) 96, 629, 723
SapI GCTCTTC 1 cut(s) 73
SaqAI TTAA 2 cut(s) 339, 431
SatI GCNGC 2 cut(s) 387, 745
Sau3AI GATC 2 cut(s) 133, 810
Sau96I GGNCC 2 cut(s) 242, 277
ScaI AGTACT 1 cut(s) 770
SchI GAGTC 2 cut(s) 433, 784
ScrFI CCNGG 1 cut(s) 843
SfaNI GCATC 3 cut(s) 398, 488, 556
SinI GGWCC 2 cut(s) 242, 277
SmiMI CAYNNNNRTG 3 cut(s) 96, 629, 723
Sse9I AATT 4 cut(s) 78, 154, 305, 457
SseBI AGGCCT 1 cut(s) 702
SsiI CCGC 6 cut(s) 30, 56, 102, 744, 825, 846
SspMI CTAG 3 cut(s) 288, 530, 674
StuI AGGCCT 1 cut(s) 702
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 4 cut(s) 97, 235, 362, 508
TaaI ACNGT 2 cut(s) 349, 631
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 132, 300, 577
TasI AATT 4 cut(s) 78, 154, 305, 457
TatI WGTACW 1 cut(s) 768
TauI GCSGC 1 cut(s) 747
TfiI GAWTC 4 cut(s) 74, 410, 554, 574
Tru1I TTAA 2 cut(s) 339, 431
Tru9I TTAA 2 cut(s) 339, 431
TscAI CASTG 3 cut(s) 354, 576, 636
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 711, 846
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 3 cut(s) 354, 576, 636
VpaK11BI GGWCC 2 cut(s) 242, 277
XapI RAATTY 1 cut(s) 154
XceI RCATGY 1 cut(s) 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 3 cut(s) 288, 530, 674
ZraI GACGTC 1 cut(s) 130
ZrmI AGTACT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.