Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001705

Physical Location & Seq

Genomic Coordinates Forward (+)

3389509 .. 3393189

Gene Structure 3681 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1046300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGATTTTCTCAAAAAAGCCAAGGTGGTACGATTTCGAAGCCACCACAACAAATACCTATTGGCCGACGACGATCAAGTGTCGGTATGGCAAAGCTGCGACGGAACAGTGCAGAACACGCGGTGGACGGTGGAGCTAATTAGCGAAAATCCAAACGCATTGCGATTGAAGAGCTGTTTTGGGAAATACTTAACGGCGTTAAGTACGCCTTTTCTTCTCGGAATCAAATGTAACAAAGTCCTGCAAACCATGCCAAAGACGTTGGATTCAATAGTGGAATGGGAGCCGGTAAGAGACGGGTACGTGTGGAAGCTGAAGGCGGCCAACGGGCAATTCTTGAGTGCAAGCGGGTGTAGCGTACCGCCGTGGAAAGACACCATCACGCACGACAATCCGCGTACAGGTACGACGAGAAATTGGGTTCTGTGGGATGTGGATGTTGTGGAGATTCGAGAACAACAACAACAACAAGAACAAACGAATGTTGATAATGTCAATGATAGTTCTAGTCGTTATAGTACTACTGCAACACAAGATCCAACAGAACCAGAACCACGGTCTGTTTCGCGGTCGGAATCTTCACAAACACAGCCAGACTCTCCAGCCGTCGTTGATCTTTCGTCAACCAAAGATCGTACACATATGGAGTTTTTCAAAAGAGCCAGAGCAGTACGCTTCCGGAGCCACCACGACAAATATCTGTTAGCAGACGAGGATCAAGAGACTGTATTCCAAGATCGCGATGGCACGGTGAAGAATGCCAAATGGACGGTGGAGCTTGTCGAAAATGCAAACACTTTACGGTTCAAAAGCTGTTATGGCAAGTACTTAACAGCCTCTAGCTTGCCATTCCGTCTTGGATTGCGAGGCAAGAAAGTTCTGCAAACCCTGCCAAAGAGATTAGATTCGTCCCTGGAATGGGAACCTATAAAAGAAGGACACCAGGTCCGGCTCAAGACGCCCTATGGTCAGTTCTTGCGCGCAAATGGGGGCGTACCACCTTGGAGAAACTCTGTCACCCATGATGTCCCACATAGAACTGCATCCCAGGATTGGGTTCTGTGGGATGTAGATGTTACAGATATTCGGGTTGAATCGCCAGAACGTCACCCGGTGGCGAAACCCCCTCCTCCACCACCAGATGCATTACTTTCGGAGTCTTCAGCACCTTCAGAACCGACATCTCCATCTGAGATTGACCTTAGAACGCCGAAGGCTTCCAAACGGGAGTCAAGCAAGTCATTTGATCATGATTCGCCGAAGAAAGATCCAGGGAGAATGATCTACTATCATGTGGCTAATGAGAAAGGAGACGTTGAGGATTCATCGGAAGAGTTTTCCTTCCCATTCAAGGGTAGTGATGTAGAGGGGTTGAAGCAAAAGTTGAAGGAAGAAACCGGGCTGGAGGAAATTGATGTGTGTTCACGCAATCCTCTGAATGGACAACTATATCCCCTTCGCTTGCATCTTCCTCCAAACAACCATGATATGCATATCGTTGTAGTTCTGTCATTGTCCGAAGGAAGTGAACTTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF569

Protein Analysis

513

Amino Acids

58.14

Weight (kDa)

6.14

Isoelectric Point (pI)

52.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF569	PF04601	1 - 143	3.7e-51	Beta-trefoil domain of unknown function (DUF569)
DUF569	PF04601	215 - 355	5.4e-67	Beta-trefoil domain of unknown function (DUF569)
Ubiq_DUF_assoc	PF22932	425 - 502	2e-09	DUF569 associated ubiquitin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001705)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01311 AT3G01311 AT3G28630 AT3G28630
fragaria_vesca	FvH4_6g49410 FvH4_6g49420
malus_domestica	MD09G1045600.v1.1 MD17G1046300.v1.1
prunus_persica	Prupe.3G273000_v2.0.a1 Prupe.3G273000_v2.0.a1 Prupe.3G273000_v2.0.a1 Prupe.3G273000_v2.0.a1 Prupe.3G273100_v2.0.a1
pyrus_communis	pycom111g03500
rosa_chinensis	RchiOBHm_Chr2g0169291 RchiOBHm_Chr2g0169301 RchiOBHm_Chr2g0169311
rosa_laevigata	RLG00000021852 RLG00000021853 RLG00000021854
rosa_multiflora	Rmu_co8313715.1_g000001 Rmu_ssc0000399.1_g000006 Rmu_ssc0000399.1_g000007 Rmu_ssc0000399.1_g000010
rosa_roxburghii	Rroxscaffold_2G00082250 Rroxscaffold_2G00082270 Rroxscaffold_2G00082280
rosa_rugosa	Rorug02G0538600 Rorug02G0538700 Rorug02G0538800 Rorug02G0538900 Rorug02G0539000 Rorug02G0539100 Rorug02G0539300 Rorug02G0539300
rosa_samantha	Rh2AG610400 Rh2AG610500 Rh2AG610600 Rh2BG623300 Rh2BG623600 Rh2BG623800 Rh2CG591700 Rh2CG592000 Rh2CG592100 Rh2DG634300 Rh2DG634600 Rh2DG634700
rosa_wichuraiana	Rw2G050620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	5 cut(s)	121, 397, 568, 741, 981
AccIII	TCCGGA	1 cut(s)	678
AciI	CCGC	6 cut(s)	121, 320, 348, 362, 395, 568
AclWI	GGATC	3 cut(s)	530, 723, 1262
AcoI	YGGCCR	2 cut(s)	63, 321
AcuI	CTGAAG	3 cut(s)	335, 1146, 1155
AcyI	GRCGYC	1 cut(s)	959
AdeI	CACNNNGTG	2 cut(s)	123, 1114
AfiI	CCNNNNNNNGG	8 cut(s)	401, 918, 919, 1053, 1054, 1351, 1352, 1439
AflIII	ACRYGT	1 cut(s)	303
AgsI	TTSAA	8 cut(s)	169, 270, 655, 808, 1094, 1351, 1375, 1387
AhdI	GACNNNNNGTC	1 cut(s)	944
AjnI	CCWGG	4 cut(s)	912, 942, 1047, 1270
AjuI	GAANNNNNNNTTGG	2 cut(s)	317, 349
AluBI	AGCT	7 cut(s)	96, 136, 174, 313, 778, 813, 843
AluI	AGCT	7 cut(s)	96, 136, 174, 313, 778, 813, 843
Alw26I	GTCTC	3 cut(s)	288, 716, 1305
AlwI	GGATC	3 cut(s)	530, 723, 1262
Aor13HI	TCCGGA	1 cut(s)	678
AoxI	GGCC	2 cut(s)	63, 321
ApeKI	GCWGC	1 cut(s)	96
AspLEI	GCGC	2 cut(s)	981, 983
AspS9I	GGNCC	1 cut(s)	946
AsuC2I	CCSGG	2 cut(s)	1112, 1399
AsuHPI	GGTGA	3 cut(s)	763, 1009, 1100
AsuII	TTCGAA	1 cut(s)	37
AvaII	GGWCC	1 cut(s)	946
BbsI	GAAGAC	1 cut(s)	1152
BbvI	GCAGC	1 cut(s)	83
BccI	CCATC	3 cut(s)	386, 737, 1195
BceAI	ACGGC	3 cut(s)	210, 349, 590
BcgI	CGANNNNNNTGC	2 cut(s)	1134, 1168
BciT130I	CCWGG	4 cut(s)	914, 944, 1049, 1272
BclI	TGATCA	1 cut(s)	1246
BcnI	CCSGG	2 cut(s)	1112, 1399
BcoDI	GTCTC	3 cut(s)	288, 716, 1305
BfaI	CTAG	2 cut(s)	507, 840
BisI	GCNGC	2 cut(s)	97, 321
BlsI	GCNGC	2 cut(s)	98, 322
BmcAI	AGTACT	2 cut(s)	520, 827
Bme1390I	CCNGG	6 cut(s)	914, 944, 1049, 1112, 1272, 1399
Bme18I	GGWCC	1 cut(s)	946
BmeRI	GACNNNNNGTC	1 cut(s)	944
BmgT120I	GGNCC	1 cut(s)	946
BmiI	GGNNCC	3 cut(s)	285, 683, 924
BmrFI	CCNGG	6 cut(s)	914, 944, 1049, 1112, 1272, 1399
BmsI	GCATC	3 cut(s)	1052, 1132, 1474
BpiI	GAAGAC	1 cut(s)	1152
BpmI	CTGGAG	2 cut(s)	585, 1424
Bpu14I	TTCGAA	1 cut(s)	37
BpuEI	CTTGAG	2 cut(s)	358, 938
BpuMI	CCSGG	2 cut(s)	1112, 1399
BsaAI	YACGTR	1 cut(s)	304
BsaHI	GRCGYC	1 cut(s)	959
BsaJI	CCNNGG	7 cut(s)	21, 365, 554, 912, 1001, 1047, 1271
BsaWI	WCCGGW	1 cut(s)	678
Bsc4I	CCNNNNNNNGG	8 cut(s)	401, 918, 919, 1053, 1054, 1351, 1352, 1439
Bse118I	RCCGGY	1 cut(s)	286
Bse3DI	GCAATG	1 cut(s)	158
BseAI	TCCGGA	1 cut(s)	678
BseBI	CCWGG	4 cut(s)	914, 944, 1049, 1272
BseDI	CCNNGG	7 cut(s)	21, 365, 554, 912, 1001, 1047, 1271
BseGI	GGATG	4 cut(s)	436, 442, 1043, 1072
BseLI	CCNNNNNNNGG	8 cut(s)	401, 918, 919, 1053, 1054, 1351, 1352, 1439
BseMI	GCAATG	1 cut(s)	158
BseMII	CTCAG	1 cut(s)	1182
BsePI	GCGCGC	1 cut(s)	979
BseRI	GAGGAG	1 cut(s)	1119
BseXI	GCAGC	1 cut(s)	83
BsgI	GTGCAG	1 cut(s)	131
Bsh1236I	CGCG	5 cut(s)	121, 397, 568, 741, 981
Bsh1285I	CGRYCG	1 cut(s)	572
BshFI	GGCC	2 cut(s)	65, 323
BsiEI	CGRYCG	1 cut(s)	572
BsiSI	CCGG	5 cut(s)	287, 679, 949, 1112, 1398
BslFI	GGGAC	2 cut(s)	895, 1013
BslI	CCNNNNNNNGG	8 cut(s)	401, 918, 919, 1053, 1054, 1351, 1352, 1439
BsmAI	GTCTC	3 cut(s)	288, 716, 1305
BsmBI	CGTCTC	2 cut(s)	288, 1305
BsmFI	GGGAC	2 cut(s)	895, 1013
BsmI	GAATGC	1 cut(s)	763
BsnI	GGCC	2 cut(s)	65, 323
Bsp119I	TTCGAA	1 cut(s)	37
Bsp13I	TCCGGA	1 cut(s)	678
Bsp143I	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
Bsp68I	TCGCGA	1 cut(s)	741
BspACI	CCGC	6 cut(s)	121, 320, 348, 362, 395, 568
BspANI	GGCC	2 cut(s)	65, 323
BspCNI	CTCAG	1 cut(s)	1183
BspEI	TCCGGA	1 cut(s)	678
BspFNI	CGCG	5 cut(s)	121, 397, 568, 741, 981
BspHI	TCATGA	1 cut(s)	1249
BspLI	GGNNCC	3 cut(s)	285, 683, 924
BspPI	GGATC	3 cut(s)	530, 723, 1262
BspQI	GCTCTTC	1 cut(s)	164
BspT104I	TTCGAA	1 cut(s)	37
BsrDI	GCAATG	1 cut(s)	158
BsrFI	RCCGGY	1 cut(s)	286
BssAI	RCCGGY	1 cut(s)	286
BssECI	CCNNGG	7 cut(s)	21, 365, 554, 912, 1001, 1047, 1271
BssHII	GCGCGC	1 cut(s)	979
BssMI	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
BssNI	GRCGYC	1 cut(s)	959
BssT1I	CCWWGG	2 cut(s)	21, 1001
Bst2UI	CCWGG	4 cut(s)	914, 944, 1049, 1272
Bst4CI	ACNGT	7 cut(s)	109, 130, 558, 727, 751, 772, 804
Bst6I	CTCTTC	2 cut(s)	164, 1326
BstACI	GRCGYC	1 cut(s)	959
BstAPI	GCANNNNNTGC	2 cut(s)	250, 889
BstBAI	YACGTR	1 cut(s)	304
BstBI	TTCGAA	1 cut(s)	37
BstC8I	GCNNGC	4 cut(s)	346, 845, 981, 1463
BstDEI	CTNAG	2 cut(s)	1191, 1202
BstDSI	CCRYGG	2 cut(s)	365, 554
BstF5I	GGATG	4 cut(s)	436, 442, 1043, 1072
BstFNI	CGCG	5 cut(s)	121, 397, 568, 741, 981
BstHHI	GCGC	2 cut(s)	981, 983
BstKTI	GATC	9 cut(s)	76, 538, 616, 634, 718, 739, 1249, 1270, 1284
BstMAI	GTCTC	3 cut(s)	288, 716, 1305
BstMBI	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
BstMCI	CGRYCG	1 cut(s)	572
BstMWI	GCNNNNNNNGC	7 cut(s)	118, 250, 354, 681, 819, 889, 958
BstNI	CCWGG	4 cut(s)	914, 944, 1049, 1272
BstSCI	CCNGG	6 cut(s)	912, 942, 1047, 1110, 1270, 1397
BstUI	CGCG	5 cut(s)	121, 397, 568, 741, 981
BstV1I	GCAGC	1 cut(s)	83
BstV2I	GAAGAC	1 cut(s)	1152
BstX2I	RGATCY	2 cut(s)	535, 1267
BstYI	RGATCY	2 cut(s)	535, 1267
BsuRI	GGCC	2 cut(s)	65, 323
BtgI	CCRYGG	2 cut(s)	365, 554
BtgZI	GCGATG	1 cut(s)	756
BtsCI	GGATG	4 cut(s)	436, 442, 1043, 1072
BtsIMutI	CAGTG	1 cut(s)	114
BtuMI	TCGCGA	1 cut(s)	741
Cac8I	GCNNGC	4 cut(s)	346, 845, 981, 1463
CciI	TCATGA	1 cut(s)	1249
CfoI	GCGC	2 cut(s)	981, 983
Cfr10I	RCCGGY	1 cut(s)	286
Cfr13I	GGNCC	1 cut(s)	946
CseI	GACGC	1 cut(s)	967
CsiI	ACCWGGT	1 cut(s)	942
CviAII	CATG	5 cut(s)	250, 1022, 1250, 1292, 1484
DdeI	CTNAG	2 cut(s)	1191, 1202
DpnI	GATC	9 cut(s)	75, 537, 615, 633, 717, 738, 1248, 1269, 1283
DpnII	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
DraIII	CACNNNGTG	2 cut(s)	123, 1114
DriI	GACNNNNNGTC	1 cut(s)	944
EaeI	YGGCCR	2 cut(s)	63, 321
Eam1104I	CTCTTC	2 cut(s)	164, 1326
Eam1105I	GACNNNNNGTC	1 cut(s)	944
EarI	CTCTTC	2 cut(s)	164, 1326
Eco130I	CCWWGG	2 cut(s)	21, 1001
Eco47I	GGWCC	1 cut(s)	946
Eco57I	CTGAAG	3 cut(s)	335, 1146, 1155
EcoRII	CCWGG	4 cut(s)	912, 942, 1047, 1270
EcoT14I	CCWWGG	2 cut(s)	21, 1001
EcoT22I	ATGCAT	2 cut(s)	1147, 1494
ErhI	CCWWGG	2 cut(s)	21, 1001
Esp3I	CGTCTC	2 cut(s)	288, 1305
FaeI	CATG	5 cut(s)	253, 1025, 1253, 1295, 1487
FalI	AAGNNNNNCTT	2 cut(s)	193, 225
FaqI	GGGAC	2 cut(s)	895, 1013
FatI	CATG	5 cut(s)	249, 1021, 1249, 1291, 1483
FauI	CCCGC	1 cut(s)	341
FauNDI	CATATG	1 cut(s)	642
FbaI	TGATCA	1 cut(s)	1246
Fnu4HI	GCNGC	2 cut(s)	97, 321
FokI	GGATG	4 cut(s)	443, 449, 1030, 1079
Fsp4HI	GCNGC	2 cut(s)	97, 321
FspBI	CTAG	2 cut(s)	507, 840
GlaI	GCGC	2 cut(s)	980, 982
GluI	GCNGC	2 cut(s)	97, 321
GsuI	CTGGAG	2 cut(s)	585, 1424
HaeIII	GGCC	2 cut(s)	65, 323
HapII	CCGG	5 cut(s)	287, 679, 949, 1112, 1398
HgaI	GACGC	1 cut(s)	967
HhaI	GCGC	2 cut(s)	981, 983
Hin1I	GRCGYC	1 cut(s)	959
Hin1II	CATG	5 cut(s)	253, 1025, 1253, 1295, 1487
Hin6I	GCGC	2 cut(s)	979, 981
HinP1I	GCGC	2 cut(s)	979, 981
HincII	GTYRAC	1 cut(s)	624
HindII	GTYRAC	1 cut(s)	624
HpaII	CCGG	5 cut(s)	287, 679, 949, 1112, 1398
HphI	GGTGA	3 cut(s)	763, 1009, 1100
Hpy166II	GTNNAC	5 cut(s)	126, 624, 638, 1424, 1529
Hpy188I	TCNGA	8 cut(s)	221, 574, 1156, 1174, 1192, 1330, 1437, 1519
Hpy188III	TCNNGA	7 cut(s)	337, 452, 679, 719, 740, 955, 1250
Hpy8I	GTNNAC	5 cut(s)	126, 624, 638, 1424, 1529
Hpy99I	CGWCG	5 cut(s)	71, 74, 104, 412, 611
HpyAV	CCTTC	8 cut(s)	310, 929, 1179, 1207, 1351, 1381, 1466, 1514
HpyCH4III	ACNGT	7 cut(s)	109, 130, 558, 727, 751, 772, 804
HpyCH4IV	ACGT	4 cut(s)	260, 303, 1105, 1314
HpyF10VI	GCNNNNNNNGC	7 cut(s)	118, 250, 354, 681, 819, 889, 958
HpyF3I	CTNAG	2 cut(s)	1191, 1202
HpySE526I	ACGT	4 cut(s)	260, 303, 1105, 1314
Hsp92I	GRCGYC	1 cut(s)	959
Hsp92II	CATG	5 cut(s)	253, 1025, 1253, 1295, 1487
HspAI	GCGC	2 cut(s)	979, 981
Kpn2I	TCCGGA	1 cut(s)	678
Ksp22I	TGATCA	1 cut(s)	1246
Kzo9I	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
LguI	GCTCTTC	1 cut(s)	164
LmnI	GCTCC	4 cut(s)	133, 283, 681, 775
Lsp1109I	GCAGC	1 cut(s)	83
LweI	GCATC	3 cut(s)	1052, 1132, 1474
MabI	ACCWGGT	1 cut(s)	942
MaeI	CTAG	2 cut(s)	507, 840
MaeII	ACGT	4 cut(s)	260, 303, 1105, 1314
MaeIII	GTNAC	4 cut(s)	230, 1015, 1075, 1106
MalI	GATC	9 cut(s)	75, 537, 615, 633, 717, 738, 1248, 1269, 1283
MboI	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
MboII	GAAGA	9 cut(s)	181, 206, 570, 766, 1152, 1273, 1343, 1403, 1460
MflI	RGATCY	2 cut(s)	535, 1267
MluCI	AATT	4 cut(s)	138, 332, 415, 1410
MlyI	GAGTC	3 cut(s)	590, 1166, 1238
MmeI	TCCRAC	3 cut(s)	243, 552, 563
Mph1103I	ATGCAT	2 cut(s)	1147, 1494
MroI	TCCGGA	1 cut(s)	678
MseI	TTAA	3 cut(s)	191, 200, 830
MspI	CCGG	5 cut(s)	287, 679, 949, 1112, 1398
MspR9I	CCNGG	6 cut(s)	914, 944, 1049, 1112, 1272, 1399
Mva1269I	GAATGC	1 cut(s)	763
MvaI	CCWGG	4 cut(s)	914, 944, 1049, 1272
MvnI	CGCG	5 cut(s)	121, 397, 568, 741, 981
MwoI	GCNNNNNNNGC	7 cut(s)	118, 250, 354, 681, 819, 889, 958
NciI	CCSGG	2 cut(s)	1112, 1399
NdeI	CATATG	1 cut(s)	642
NdeII	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
NlaIII	CATG	5 cut(s)	253, 1025, 1253, 1295, 1487
NlaIV	GGNNCC	3 cut(s)	285, 683, 924
NmuCI	GTSAC	2 cut(s)	1015, 1106
NruI	TCGCGA	1 cut(s)	741
NsiI	ATGCAT	2 cut(s)	1147, 1494
NspV	TTCGAA	1 cut(s)	37
PagI	TCATGA	1 cut(s)	1249
PauI	GCGCGC	1 cut(s)	979
PciSI	GCTCTTC	1 cut(s)	164
PctI	GAATGC	1 cut(s)	763
PfeI	GAWTC	8 cut(s)	222, 266, 448, 575, 905, 1094, 1253, 1322
PkrI	GCNGC	2 cut(s)	98, 322
PleI	GAGTC	3 cut(s)	590, 1165, 1237
PpsI	GAGTC	3 cut(s)	590, 1165, 1237
Ppu21I	YACGTR	1 cut(s)	304
Psp6I	CCWGG	4 cut(s)	912, 942, 1047, 1270
PspGI	CCWGG	4 cut(s)	912, 942, 1047, 1270
PspN4I	GGNNCC	3 cut(s)	285, 683, 924
PspPI	GGNCC	1 cut(s)	946
PsuI	RGATCY	2 cut(s)	535, 1267
PteI	GCGCGC	1 cut(s)	979
RruI	TCGCGA	1 cut(s)	741
SapI	GCTCTTC	1 cut(s)	164
SaqAI	TTAA	3 cut(s)	191, 200, 830
SatI	GCNGC	2 cut(s)	97, 321
Sau3AI	GATC	9 cut(s)	73, 535, 613, 631, 715, 736, 1246, 1267, 1281
Sau96I	GGNCC	1 cut(s)	946
ScaI	AGTACT	2 cut(s)	520, 827
SchI	GAGTC	3 cut(s)	590, 1166, 1238
ScrFI	CCNGG	6 cut(s)	914, 944, 1049, 1112, 1272, 1399
SexAI	ACCWGGT	1 cut(s)	942
SfaNI	GCATC	3 cut(s)	1052, 1132, 1474
SfuI	TTCGAA	1 cut(s)	37
SinI	GGWCC	1 cut(s)	946
SmlI	CTYRAG	2 cut(s)	337, 953
SmoI	CTYRAG	2 cut(s)	337, 953
Sse9I	AATT	4 cut(s)	138, 332, 415, 1410
SsiI	CCGC	6 cut(s)	121, 320, 348, 362, 395, 568
SspMI	CTAG	2 cut(s)	507, 840
StyD4I	CCNGG	6 cut(s)	912, 942, 1047, 1110, 1270, 1397
StyI	CCWWGG	2 cut(s)	21, 1001
TaaI	ACNGT	7 cut(s)	109, 130, 558, 727, 751, 772, 804
TaiI	ACGT	4 cut(s)	263, 306, 1108, 1317
TaqI	TCGA	3 cut(s)	37, 451, 783
TasI	AATT	4 cut(s)	138, 332, 415, 1410
TatI	WGTACW	2 cut(s)	518, 825
TauI	GCSGC	1 cut(s)	323
TfiI	GAWTC	8 cut(s)	222, 266, 448, 575, 905, 1094, 1253, 1322
Tru1I	TTAA	3 cut(s)	191, 200, 830
Tru9I	TTAA	3 cut(s)	191, 200, 830
TscAI	CASTG	1 cut(s)	114
TseFI	GTSAC	2 cut(s)	1015, 1106
TseI	GCWGC	1 cut(s)	96
Tsp45I	GTSAC	2 cut(s)	1015, 1106
TspDTI	ATGAA	1 cut(s)	1314
TspGWI	ACGGA	2 cut(s)	117, 842
TspRI	CASTG	1 cut(s)	114
VpaK11BI	GGWCC	1 cut(s)	946
XcmI	CCANNNNNNNNNTGG	2 cut(s)	740, 769
XspI	CTAG	2 cut(s)	507, 840
ZrmI	AGTACT	2 cut(s)	520, 827
Zsp2I	ATGCAT	2 cut(s)	1147, 1494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.