pycom111g03500

Domain of unknown function (DUF569)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001705
Physical Location & Seq
Forward (+)
2146850 .. 2147877
1028 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03500.2

Sequence Viewer

Length: 987 bp
ATGTGCTTAATCACAGTACTTGATCGGTCTTATTGTTTCCTAGAGCTACATATATATATGGAGTTCTTCAAAAAAGCCAGATCAGTACGCTTCCGGAGCCACCACGGAAAATATCTATTAGCAGACGAGGATCAAGAGACTGTTTTCCAAGATCGCGAAGGCACGGCAAAGAATGCCAAATGGATGGTGGAGTTTGTCGAAAATGCAAACACTTTACGGTTCAAAAGCTGTTATGGCAAGTACTTAACAGCCTCTAGCATGCCATACCGTCTTGGATTGCGAGGCAAGAAAGTTCTGCAAACACTGCCAAAGAGATTAGATTCTTCCCTTGAATGGGAGCCTGTAAAAGAAGGATATCAGGTCCGGCTCAGGACGCCCTATGGCCAGTTCTTGCGCGCAAATGGGGGCGTACCGCCTTGGAGAAACTCTGTCACCCATGATATCCCTCAAAGAACTGCAAAACAGGATTGGGTTCTGTGGGATGTAGATGTTATAGAGGTTCGGGTTGAATCTCCAGAACGTCACCCAGTGGCGAAACCCCCTCCTCCACAACCAGATGCATTGCCTTCGAAGTCTTCAGCACCTTCAGAACCTACATCTCCATCTAAGATTGAGCTTAGGATGCCGAGAGCTTCCAAACACCAGTTTAATTATATTTTTGGCTTCTTATTGTTTGTTCTTAAACGCAGTCAAGCGAGTCATTTGATCGATGATTCGCGAAAGAAAGATGCAGGGAGAATGATCTACTATTGTGTTGCTAATAAGAAAGGAGACGTTGAGGATTCATTGGAAGAGTTTTCCTTCCCATTCAAGGGTAGTGAGGTAGAAGGGTTGAAGCAAAAGTTGAAGGAAGAAACCGGATTGGAGGAAATTGTTGTGTGTTCACGCAATCCTCTGACTGGAAAACTTTATCCCCTTCGCTTACATCTTCCGCCAAACAACCACGATATGCATATCATTGTAGTTCCGTCATCGTCCAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF569

Protein Analysis

329

Amino Acids

37.74

Weight (kDa)

9.24

Isoelectric Point (pI)

59.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 156, 396, 718
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 2 cut(s) 413, 932
AclWI GGATC 1 cut(s) 138
AcoI YGGCCR 1 cut(s) 382
AcuI CTGAAG 2 cut(s) 561, 570
AcyI GRCGYC 1 cut(s) 374
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 4 cut(s) 18, 87, 242, 411
AfiI CCNNNNNNNGG 5 cut(s) 333, 334, 811, 812, 899
AgsI TTSAA 7 cut(s) 70, 223, 332, 509, 811, 835, 847
AluBI AGCT 4 cut(s) 46, 228, 616, 632
AluI AGCT 4 cut(s) 46, 228, 616, 632
Alw26I GTCTC 2 cut(s) 131, 765
AlwI GGATC 1 cut(s) 138
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 382
AspLEI GCGC 2 cut(s) 396, 398
AspS9I GGNCC 1 cut(s) 361
AsuHPI GGTGA 2 cut(s) 424, 515
AsuII TTCGAA 1 cut(s) 569
AvaII GGWCC 1 cut(s) 361
BalI TGGCCA 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 567
BccI CCATC 2 cut(s) 178, 610
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BcoDI GTCTC 2 cut(s) 131, 765
BfaI CTAG 2 cut(s) 41, 255
BmcAI AGTACT 2 cut(s) 18, 242
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 2 cut(s) 98, 339
BmrI ACTGGG 1 cut(s) 521
BmsI GCATC 3 cut(s) 547, 612, 718
BmuI ACTGGG 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 567
BpmI CTGGAG 1 cut(s) 498
Bpu10I CCTNAGC 2 cut(s) 368, 617
Bpu14I TTCGAA 1 cut(s) 569
Bsa29I ATCGAT 1 cut(s) 708
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 103, 416
BsaWI WCCGGW 2 cut(s) 93, 857
Bsc4I CCNNNNNNNGG 5 cut(s) 333, 334, 811, 812, 899
Bse1I ACTGG 4 cut(s) 385, 527, 643, 904
Bse3DI GCAATG 1 cut(s) 560
BseAI TCCGGA 1 cut(s) 93
BseCI ATCGAT 1 cut(s) 708
BseDI CCNNGG 2 cut(s) 103, 416
BseGI GGATG 3 cut(s) 189, 487, 627
BseLI CCNNNNNNNGG 5 cut(s) 333, 334, 811, 812, 899
BseMI GCAATG 1 cut(s) 560
BseMII CTCAG 1 cut(s) 382
BseNI ACTGG 4 cut(s) 385, 527, 643, 904
BsePI GCGCGC 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 534
Bsh1236I CGCG 3 cut(s) 156, 396, 718
BshFI GGCC 1 cut(s) 384
BshVI ATCGAT 1 cut(s) 708
BsiSI CCGG 3 cut(s) 94, 364, 858
BslI CCNNNNNNNGG 5 cut(s) 333, 334, 811, 812, 899
BsmAI GTCTC 2 cut(s) 131, 765
BsmBI CGTCTC 1 cut(s) 765
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 1 cut(s) 384
Bsp119I TTCGAA 1 cut(s) 569
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 6 cut(s) 22, 80, 130, 151, 705, 741
Bsp68I TCGCGA 2 cut(s) 156, 718
BspACI CCGC 2 cut(s) 413, 932
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 1 cut(s) 381
BspDI ATCGAT 1 cut(s) 708
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 3 cut(s) 156, 396, 718
BspLI GGNNCC 2 cut(s) 98, 339
BspPI GGATC 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 560
BsrI ACTGG 4 cut(s) 385, 527, 643, 904
BssECI CCNNGG 2 cut(s) 103, 416
BssHII GCGCGC 1 cut(s) 394
BssMI GATC 6 cut(s) 22, 80, 130, 151, 705, 741
BssNI GRCGYC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 416
Bst4CI ACNGT 4 cut(s) 16, 142, 219, 269
Bst6I CTCTTC 1 cut(s) 786
BstACI GRCGYC 1 cut(s) 374
BstAPI GCANNNNNTGC 2 cut(s) 173, 304
BstBI TTCGAA 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 260, 396
BstDEI CTNAG 3 cut(s) 368, 606, 617
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 3 cut(s) 189, 487, 627
BstFNI CGCG 3 cut(s) 156, 396, 718
BstHHI GCGC 2 cut(s) 396, 398
BstKTI GATC 6 cut(s) 25, 83, 133, 154, 708, 744
BstMAI GTCTC 2 cut(s) 131, 765
BstMBI GATC 6 cut(s) 22, 80, 130, 151, 705, 741
BstMWI GCNNNNNNNGC 6 cut(s) 96, 173, 234, 304, 373, 622
BstNSI RCATGY 1 cut(s) 262
BstUI CGCG 3 cut(s) 156, 396, 718
BstV2I GAAGAC 1 cut(s) 567
BstXI CCANNNNNNTGG 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 708
BsuRI GGCC 1 cut(s) 384
BsuTUI ATCGAT 1 cut(s) 708
BtgI CCRYGG 1 cut(s) 103
BtsCI GGATG 3 cut(s) 189, 487, 627
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 2 cut(s) 302, 534
BtuMI TCGCGA 2 cut(s) 156, 718
Cac8I GCNNGC 2 cut(s) 260, 396
CfoI GCGC 2 cut(s) 396, 398
Cfr13I GGNCC 1 cut(s) 361
ClaI ATCGAT 1 cut(s) 708
CseI GACGC 1 cut(s) 382
Csp6I GTAC 4 cut(s) 17, 86, 241, 410
CviAII CATG 2 cut(s) 259, 437
CviQI GTAC 4 cut(s) 17, 86, 241, 410
DdeI CTNAG 3 cut(s) 368, 606, 617
DpnI GATC 6 cut(s) 24, 82, 132, 153, 707, 743
DpnII GATC 6 cut(s) 22, 80, 130, 151, 705, 741
DraIII CACNNNGTG 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
EciI GGCGGA 1 cut(s) 921
Eco130I CCWWGG 1 cut(s) 416
Eco32I GATATC 2 cut(s) 356, 442
Eco47I GGWCC 1 cut(s) 361
Eco57I CTGAAG 2 cut(s) 561, 570
EcoRV GATATC 2 cut(s) 356, 442
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 2 cut(s) 562, 954
ErhI CCWWGG 1 cut(s) 416
Esp3I CGTCTC 1 cut(s) 765
FaeI CATG 2 cut(s) 262, 440
FatI CATG 2 cut(s) 258, 436
FokI GGATG 3 cut(s) 196, 494, 634
FspBI CTAG 2 cut(s) 41, 255
GlaI GCGC 2 cut(s) 395, 397
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 1 cut(s) 384
HapII CCGG 3 cut(s) 94, 364, 858
HgaI GACGC 1 cut(s) 382
HhaI GCGC 2 cut(s) 396, 398
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 2 cut(s) 262, 440
Hin6I GCGC 2 cut(s) 394, 396
HinP1I GCGC 2 cut(s) 394, 396
HinfI GANTC 5 cut(s) 320, 509, 697, 713, 782
HpaII CCGG 3 cut(s) 94, 364, 858
HphI GGTGA 2 cut(s) 424, 515
Hpy166II GTNNAC 1 cut(s) 884
Hpy188I TCNGA 2 cut(s) 589, 897
Hpy188III TCNNGA 6 cut(s) 94, 134, 155, 370, 515, 717
Hpy8I GTNNAC 1 cut(s) 884
HpyAV CCTTC 8 cut(s) 152, 344, 576, 594, 811, 821, 841, 926
HpyCH4III ACNGT 4 cut(s) 16, 142, 219, 269
HpyCH4IV ACGT 2 cut(s) 520, 774
HpyCH4V TGCA 6 cut(s) 206, 298, 458, 560, 731, 952
HpyF10VI GCNNNNNNNGC 6 cut(s) 96, 173, 234, 304, 373, 622
HpyF3I CTNAG 3 cut(s) 368, 606, 617
HpySE526I ACGT 2 cut(s) 520, 774
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 2 cut(s) 262, 440
HspAI GCGC 2 cut(s) 394, 396
Kpn2I TCCGGA 1 cut(s) 93
Kzo9I GATC 6 cut(s) 22, 80, 130, 151, 705, 741
LmnI GCTCC 2 cut(s) 96, 337
LweI GCATC 3 cut(s) 547, 612, 718
MaeI CTAG 2 cut(s) 41, 255
MaeII ACGT 2 cut(s) 520, 774
MaeIII GTNAC 2 cut(s) 430, 521
MalI GATC 6 cut(s) 24, 82, 132, 153, 707, 743
MboI GATC 6 cut(s) 22, 80, 130, 151, 705, 741
MboII GAAGA 6 cut(s) 58, 315, 567, 803, 863, 920
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 2 cut(s) 649, 870
MluNI TGGCCA 1 cut(s) 384
MlyI GAGTC 1 cut(s) 706
Mox20I TGGCCA 1 cut(s) 384
Mph1103I ATGCAT 2 cut(s) 562, 954
MroI TCCGGA 1 cut(s) 93
MscI TGGCCA 1 cut(s) 384
MseI TTAA 4 cut(s) 8, 245, 648, 681
Msp20I TGGCCA 1 cut(s) 384
MspI CCGG 3 cut(s) 94, 364, 858
Mva1269I GAATGC 1 cut(s) 178
MvnI CGCG 3 cut(s) 156, 396, 718
MwoI GCNNNNNNNGC 6 cut(s) 96, 173, 234, 304, 373, 622
NdeII GATC 6 cut(s) 22, 80, 130, 151, 705, 741
NlaIII CATG 2 cut(s) 262, 440
NlaIV GGNNCC 2 cut(s) 98, 339
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 2 cut(s) 430, 521
NruI TCGCGA 2 cut(s) 156, 718
NsiI ATGCAT 2 cut(s) 562, 954
NspI RCATGY 1 cut(s) 262
NspV TTCGAA 1 cut(s) 569
PaeI GCATGC 1 cut(s) 262
PauI GCGCGC 1 cut(s) 394
PctI GAATGC 1 cut(s) 178
PfeI GAWTC 4 cut(s) 320, 509, 713, 782
PleI GAGTC 1 cut(s) 705
PpsI GAGTC 1 cut(s) 705
PspN4I GGNNCC 2 cut(s) 98, 339
PspPI GGNCC 1 cut(s) 361
PteI GCGCGC 1 cut(s) 394
RruI TCGCGA 2 cut(s) 156, 718
RsaI GTAC 4 cut(s) 18, 87, 242, 411
RsaNI GTAC 4 cut(s) 17, 86, 241, 410
SaqAI TTAA 4 cut(s) 8, 245, 648, 681
Sau3AI GATC 6 cut(s) 22, 80, 130, 151, 705, 741
Sau96I GGNCC 1 cut(s) 361
ScaI AGTACT 2 cut(s) 18, 242
SchI GAGTC 1 cut(s) 706
SfaNI GCATC 3 cut(s) 547, 612, 718
SfuI TTCGAA 1 cut(s) 569
SinI GGWCC 1 cut(s) 361
SphI GCATGC 1 cut(s) 262
Sse9I AATT 2 cut(s) 649, 870
SsiI CCGC 2 cut(s) 413, 932
SspMI CTAG 2 cut(s) 41, 255
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 4 cut(s) 16, 142, 219, 269
TaiI ACGT 2 cut(s) 523, 777
TaqI TCGA 3 cut(s) 198, 569, 708
TaqII GACCGA 1 cut(s) 15
TasI AATT 2 cut(s) 649, 870
TatI WGTACW 2 cut(s) 16, 240
TfiI GAWTC 4 cut(s) 320, 509, 713, 782
Tru1I TTAA 4 cut(s) 8, 245, 648, 681
Tru9I TTAA 4 cut(s) 8, 245, 648, 681
TscAI CASTG 2 cut(s) 309, 534
TseFI GTSAC 2 cut(s) 430, 521
Tsp45I GTSAC 2 cut(s) 430, 521
TspDTI ATGAA 1 cut(s) 774
TspGWI ACGGA 2 cut(s) 120, 957
TspRI CASTG 2 cut(s) 309, 534
VpaK11BI GGWCC 1 cut(s) 361
XceI RCATGY 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 2 cut(s) 41, 255
ZrmI AGTACT 2 cut(s) 18, 242
Zsp2I ATGCAT 2 cut(s) 562, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.