MD17G1222300.v1.1

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001637
Physical Location & Seq
Forward (+)
27058364 .. 27069393
11030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1222300.v1.1.491

Sequence Viewer

Length: 2931 bp
ATGTCTTGTTCCTCTGAGAAAGAGCTGGAGGAGCAGCTCAAGGAGGCTGGAAATATTCTCCTCAACCCTCCTCCTGCTACCGAGGAGCTTCTCAAAATCCTTGGTGAAGCTGAGGGTTTGTTGTCAAATGTTCAGCAAGCACCACACAGGTCCATGCAAGATGCACTCCTACCGATAATGAAGGCATTAATTTCTGATGAGCTGTTTAGGCACTCAGATATGGATGTGAAGCTATATGTTGCTTCTTGCATTACTGAGCTTACGAGAATAACAGCACCCGTTCCTCCATACAATGATGAATGGATGAAGGAAATCTTTCAGCTGACTGTAGCAGCTTTTGAGAAACTGTCATATGTTTCCAGTCCCTTTTATAGAAAGGCAGTTTCCATTCTTCATATTGTGGCAAAGGTCAGGTCAAGCTTGCTGATGCTGGACCTTGGTTGCAGTGCATTAATTCTTGAGATGTTTCAACATTTCCTTAATATAACCCAGTCCAATCATCCACATTCTGTATTTTCAGCCATGGAAACTATTATGACCATGGTTTTAGATGAAAGTGAAGAAATTCCCGTGGATCTTGTCAAAGCTCTTCTATCCAGTGTTAGAAAGGAAAATCAGACTGTTTCACCTTTTTCATGGAAATTGGGGAAGAAGGTTATAGACAATTCTTCAGCTAAGCTTAAACCTTATCTCGTGGAAGCAGTGAAGTCCATGGACACTGCTTTGGATGATTACGCGCAAATAGTGGCTTCTGTATGCCAAAATGGATGTCAAGCTGTCAAACATGATCATGTTAATGATTCTGTGAAGACTTTGGTGACCGAGGGGCCTAAGGCAATTGCTGTTTGTCCCAGAGAAGTTGAACATACTGTAGCTGCTTCTACTGGGGATGAACATATCAGGCATAAAAATTCTACTGCAGGATTACAGCATTGCCATCATACTAAACCCTCCAAGGTTATTGATTCAAGAGATAGTGCTAAACCAGACAACTACTCTTCAAAAACTATCAAATCAGCAACTGAGCCATGCTCGGTTCCCAAGAAGAGAGGCCAGAAATCAAATTCTTTGAAGGGTTCAGAAGAAGGATATGACAATGCTCAACCCAACAATTCACTCTCTCTGAACGCTGTTAAATCAGAAGAGCCATCCTCGGCCTCCAAAAAGAGAGGCAGGAAGCCAAATTCTTTAATGAACCCAGCAGAAGGCTACGACCATTCTTGGATTAGTAGTGGGAGAAAAACTCAAAAACCATCTGCCAGCAGTAAGTCTGATTACAAGGTGTCTAATCCTTCACCTACAAATGATCGTCTTCGTGCAAAGGCTGAAGTACCCTTGATGCTTAAAAATGTGAGGGAGCCCCTGGGTTCCAAACCTAAAATTGAAAGGGATATACGAGCTTCTCCATTTCTTCCGAAGAACCCTCTTGGTGGATCCCAACACATAAAGGGCCGGCCGAAGAAGAAAGATGTAGCAGACCATGCTGCTAAGCCTAGTTCCCTTTCAGCTGTGAAGCAAGAGCTCTCAATTGCTCAAATTGAGGAGAGGACTCCTAAAGAAGAAAAGCCCAAGCTGAGAAAGAAGTATAAGGGGACTGTTAACACAGGAGAAAGACTACGGGAGCAGCTTAGAGATATTAGACTTGCTGAAAAATCAAATGAGGGCTGCATTCGTTCTTCAGTAAATGTGGTCACAGATAAGGAATCTGAAGTTCCTACTGATCCTGATAAAGCATCACAAAAACAGTTGTCTTTGGATGTAGAAACGAAGAGTACCACTGATCCATCATCTCTCCGTCATGGTATTAAGAAAAGTAGGCGGAATGGTGCTTCTCGAATGGAGAGTACTGAAGCATCTGGTAGCAAGATTTTTGAGTCTGCGAAAAAAACGAATGAAGATAATGAAGGAACCTCTAAAGTGGTTCTCAAGAGGAAGCGTATCATTGAAAAGGAAGAGGCCTCTCAAATGCCTGATCTCGGTGAACGGTTGGTTGACAGAAGAATAAAGGTTTGGTGGCCACAGGACAAAAAGTTTTATGAAGGTGTTGTTTCTTCTTACGACCCTGCCAAAAAGAAACACTTGGTGTTGTATGCTGATGGGGATGAAGAAAACCTGAACCTCAAAAAGCAGCGCTGGAAATTGATTGGAGATGATTTGCCAGACGTCGAAATGGATAATCTGCTAAAACTTTCTGCTGCTTCTGATACACTACCACAAAAGAGAAAACGGAAACCAAAATCAGAACGATCAAAACGAACGCATGCTGGTCTGTCCTCAAAAAGGATATCTAGACGTGGAGTCTCAGCTGAAAGTAAATCTGCGGACTGTTTTGTACTAGGTGAGCGTAAAGTTATTGATGAACGTGATGGTGATCAAGCCAAGGAAAATGACATATTGAAGGATGATGGCCAAATATCTGCTGATAAACTGGAGGCGGATATGAAGACTGGTATTGGCTCTTGCACAGTAGGTGAGCGTAAAGTTATTGATGAAAGTGAGGATAATCAAGCCAAGGATAATGGCCAAATATCTGCTGGTGAATTGGAGGCAAATATGAAGACTGGTATTGATGAATGTGAAGGCGAGCAAGCCAAGGAAAATGACCAAACGTCTGCTGGTGAAGTGCAGGCTGATATGAAGACTGGTGTTGACTCTGCATCAGTCGGCGAGCATAAGGTTATTGATGAATGTGAGCGGGATCAAGCCAAGGAAAATGACGGATTGAAGGATAATGGCCAAATATCTGCTGGTGAATTGGACGCCAGTATGAAGGCTGATATTGACTCCGCACAGCTCACACCGGAGACTATGACCTTATCCGAGGGAGAGAGTCCCAAAGTTGATATTGAACCTTCTGGTAGGGAATGCTTCAAAGAACTGATGGAATTGGGCAAGGGTGCAGAGACTGCAAGATCACTTGACGTCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEARLI-4

Protein Analysis

977

Amino Acids

107.74

Weight (kDa)

6.65

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 28 - 231 1.4e-47 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 2166, 2925
AccBSI CCGCTC 1 cut(s) 2695
AccII CGCG 1 cut(s) 737
AciI CCGC 5 cut(s) 1819, 2321, 2435, 2695, 2787
AclWI GGATC 6 cut(s) 582, 1428, 1441, 1715, 1775, 2706
AcoI YGGCCR 5 cut(s) 1454, 2015, 2407, 2521, 2734
AcsI RAATTY 4 cut(s) 564, 910, 1063, 1183
AcuI CTGAAG 5 cut(s) 654, 1347, 1662, 1728, 1869
AcyI GRCGYC 3 cut(s) 2163, 2760, 2922
AdeI CACNNNGTG 1 cut(s) 2083
AfaI GTAC 4 cut(s) 1332, 1774, 1846, 2334
AfeI AGCGCT 1 cut(s) 2132
AfiI CCNNNNNNNGG 4 cut(s) 1205, 1430, 1976, 2280
AhdI GACNNNNNGTC 2 cut(s) 2297, 2608
AjiI CACGTC 1 cut(s) 2294
AjnI CCWGG 1 cut(s) 1362
AjuI GAANNNNNNNTTGG 2 cut(s) 1993, 2025
Alw21I GWGCWC 1 cut(s) 1524
Alw26I GTCTC 3 cut(s) 2305, 2798, 2897
AlwI GGATC 6 cut(s) 582, 1428, 1441, 1715, 1775, 2706
AlwNI CAGNNNCTG 2 cut(s) 1022, 2906
Aor51HI AGCGCT 1 cut(s) 2132
ApeKI GCWGC 8 cut(s) 34, 332, 875, 1484, 1624, 1665, 2128, 2195
ApoI RAATTY 4 cut(s) 564, 910, 1063, 1183
ArsI GACNNNNNNTTYG 4 cut(s) 398, 430, 754, 786
AseI ATTAAT 2 cut(s) 188, 452
Asp700I GAANNNNTTC 2 cut(s) 315, 564
AspLEI GCGC 2 cut(s) 739, 2133
AspS9I GGNCC 4 cut(s) 150, 433, 827, 1450
AvaII GGWCC 2 cut(s) 150, 433
AxyI CCTNAGG 1 cut(s) 831
BalI TGGCCA 4 cut(s) 2017, 2409, 2523, 2736
BamHI GGATCC 1 cut(s) 1433
BanII GRGCYC 2 cut(s) 1362, 1524
BauI CACGAG 1 cut(s) 692
BbsI GAAGAC 5 cut(s) 815, 1304, 2450, 2564, 2645
Bbv12I GWGCWC 1 cut(s) 1524
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 8 cut(s) 46, 344, 862, 1471, 1636, 1652, 2140, 2182
BccI CCATC 8 cut(s) 945, 1156, 1261, 1792, 2090, 2360, 2399, 2875
BciT130I CCWGG 1 cut(s) 1364
BclI TGATCA 2 cut(s) 787, 2371
BcoDI GTCTC 3 cut(s) 2305, 2798, 2897
BfaI CTAG 3 cut(s) 1494, 2289, 2336
BfmI CTRYAG 3 cut(s) 327, 870, 918
BfoI RGCGCY 1 cut(s) 2134
BisI GCNGC 8 cut(s) 35, 333, 876, 1485, 1625, 1666, 2129, 2196
BlpI GCTNAGC 2 cut(s) 675, 1488
BlsI GCNGC 8 cut(s) 36, 334, 877, 1486, 1626, 1667, 2130, 2197
BmcAI AGTACT 1 cut(s) 1846
Bme1390I CCNGG 1 cut(s) 1364
Bme18I GGWCC 2 cut(s) 150, 433
BmeRI GACNNNNNGTC 2 cut(s) 2297, 2608
BmgBI CACGTC 1 cut(s) 2294
BmgT120I GGNCC 4 cut(s) 150, 433, 827, 1450
BmiI GGNNCC 6 cut(s) 828, 1038, 1359, 1369, 1435, 1909
BmrFI CCNGG 1 cut(s) 1364
BmrI ACTGGG 2 cut(s) 484, 894
BmsI GCATC 6 cut(s) 151, 417, 1329, 1742, 1862, 2666
BmuI ACTGGG 2 cut(s) 484, 894
BpiI GAAGAC 5 cut(s) 815, 1304, 2450, 2564, 2645
BplI GAGNNNNNCTC 6 cut(s) 1016, 1048, 1136, 1168, 1228, 1260
BpmI CTGGAG 2 cut(s) 47, 2450
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 2 cut(s) 675, 1488
BpuEI CTTGAG 3 cut(s) 23, 479, 1910
BsaBI GATNNNNATC 1 cut(s) 2370
BsaHI GRCGYC 3 cut(s) 2163, 2760, 2922
BsaWI WCCGGW 1 cut(s) 2800
Bsc4I CCNNNNNNNGG 4 cut(s) 1205, 1430, 1976, 2280
Bse118I RCCGGY 1 cut(s) 1452
Bse1I ACTGG 9 cut(s) 360, 490, 597, 889, 2433, 2452, 2566, 2647, 2763
Bse21I CCTNAGG 1 cut(s) 831
Bse3DI GCAATG 1 cut(s) 931
Bse8I GATNNNNATC 1 cut(s) 2370
BseBI CCWGG 1 cut(s) 1364
BseJI GATNNNNATC 1 cut(s) 2370
BseLI CCNNNNNNNGG 4 cut(s) 1205, 1430, 1976, 2280
BseMI GCAATG 1 cut(s) 931
BseMII CTCAG 7 cut(s) 6, 102, 228, 246, 1014, 1565, 2316
BseNI ACTGG 9 cut(s) 360, 490, 597, 889, 2433, 2452, 2566, 2647, 2763
BseRI GAGGAG 5 cut(s) 44, 50, 60, 98, 1556
BseX3I CGGCCG 1 cut(s) 1454
BseXI GCAGC 8 cut(s) 46, 344, 862, 1471, 1636, 1652, 2140, 2182
BseYI CCCAGC 1 cut(s) 1198
BsgI GTGCAG 2 cut(s) 2645, 2919
Bsh1236I CGCG 1 cut(s) 737
Bsh1285I CGRYCG 1 cut(s) 1457
BsiEI CGRYCG 1 cut(s) 1457
BsiHKAI GWGCWC 1 cut(s) 1524
BsiSI CCGG 2 cut(s) 1453, 2801
BslFI GGGAC 4 cut(s) 348, 834, 1606, 2817
BslI CCNNNNNNNGG 4 cut(s) 1205, 1430, 1976, 2280
BsmAI GTCTC 3 cut(s) 2305, 2798, 2897
BsmFI GGGAC 4 cut(s) 348, 834, 1606, 2817
BsmI GAATGC 2 cut(s) 1668, 2870
Bsp1286I GDGCHC 2 cut(s) 1362, 1524
Bsp1720I GCTNAGC 2 cut(s) 675, 1488
Bsp19I CCATGG 3 cut(s) 522, 540, 711
BspACI CCGC 5 cut(s) 1819, 2321, 2435, 2695, 2787
BspCNI CTCAG 7 cut(s) 7, 103, 227, 247, 1015, 1566, 2315
BspFNI CGCG 1 cut(s) 737
BspLI GGNNCC 6 cut(s) 828, 1038, 1359, 1369, 1435, 1909
BspMAI CTGCAG 1 cut(s) 922
BspPI GGATC 6 cut(s) 582, 1428, 1441, 1715, 1775, 2706
BspQI GCTCTTC 2 cut(s) 594, 1137
BsrBI CCGCTC 1 cut(s) 2695
BsrDI GCAATG 1 cut(s) 931
BsrFI RCCGGY 1 cut(s) 1452
BsrI ACTGG 9 cut(s) 360, 490, 597, 889, 2433, 2452, 2566, 2647, 2763
BssAI RCCGGY 1 cut(s) 1452
BssNI GRCGYC 3 cut(s) 2163, 2760, 2922
BssSI CACGAG 1 cut(s) 692
Bst2BI CACGAG 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 1364
Bst4CI ACNGT 9 cut(s) 328, 348, 622, 871, 1597, 1746, 1986, 2327, 2467
Bst6I CTCTTC 6 cut(s) 594, 1003, 1040, 1137, 1763, 1947
BstACI GRCGYC 3 cut(s) 2163, 2760, 2922
BstAPI GCANNNNNTGC 2 cut(s) 1481, 2906
BstC8I GCNNGC 9 cut(s) 138, 422, 1261, 1454, 2262, 2585, 2589, 2628, 2669
BstDSI CCRYGG 4 cut(s) 522, 540, 570, 711
BstEII GGTNACC 1 cut(s) 817
BstENI CCTNNNNNAGG 1 cut(s) 2278
BstFNI CGCG 1 cut(s) 737
BstH2I RGCGCY 1 cut(s) 2134
BstHHI GCGC 2 cut(s) 739, 2133
BstMAI GTCTC 3 cut(s) 2305, 2798, 2897
BstMCI CGRYCG 1 cut(s) 1457
BstMWI GCNNNNNNNGC 4 cut(s) 31, 208, 1481, 2906
BstNI CCWGG 1 cut(s) 1364
BstNSI RCATGY 1 cut(s) 2264
BstPI GGTNACC 1 cut(s) 817
BstSCI CCNGG 1 cut(s) 1362
BstSFI CTRYAG 3 cut(s) 327, 870, 918
BstUI CGCG 1 cut(s) 737
BstV1I GCAGC 8 cut(s) 46, 344, 862, 1471, 1636, 1652, 2140, 2182
BstV2I GAAGAC 5 cut(s) 815, 1304, 2450, 2564, 2645
BstX2I RGATCY 2 cut(s) 574, 1433
BstYI RGATCY 2 cut(s) 574, 1433
BstZI CGGCCG 1 cut(s) 1454
Bsu36I CCTNAGG 1 cut(s) 831
BtgI CCRYGG 4 cut(s) 522, 540, 570, 711
BtrI CACGTC 1 cut(s) 2294
BtsI GCAGTG 3 cut(s) 451, 708, 717
BtsIMutI CAGTG 5 cut(s) 451, 604, 708, 717, 1776
Cac8I GCNNGC 9 cut(s) 138, 422, 1261, 1454, 2262, 2585, 2589, 2628, 2669
CaiI CAGNNNCTG 2 cut(s) 1022, 2906
CfoI GCGC 2 cut(s) 739, 2133
Cfr10I RCCGGY 1 cut(s) 1452
Cfr13I GGNCC 4 cut(s) 150, 433, 827, 1450
CseI GACGC 1 cut(s) 2768
Csp6I GTAC 4 cut(s) 1331, 1773, 1845, 2333
CviQI GTAC 4 cut(s) 1331, 1773, 1845, 2333
DraIII CACNNNGTG 1 cut(s) 2083
DriI GACNNNNNGTC 2 cut(s) 2297, 2608
EaeI YGGCCR 5 cut(s) 1454, 2015, 2407, 2521, 2734
EagI CGGCCG 1 cut(s) 1454
Eam1104I CTCTTC 6 cut(s) 594, 1003, 1040, 1137, 1763, 1947
Eam1105I GACNNNNNGTC 2 cut(s) 2297, 2608
EarI CTCTTC 6 cut(s) 594, 1003, 1040, 1137, 1763, 1947
EciI GGCGGA 2 cut(s) 1834, 2450
Ecl136II GAGCTC 1 cut(s) 1522
EclXI CGGCCG 1 cut(s) 1454
Eco147I AGGCCT 1 cut(s) 1958
Eco24I GRGCYC 2 cut(s) 1362, 1524
Eco32I GATATC 1 cut(s) 2286
Eco47I GGWCC 2 cut(s) 150, 433
Eco47III AGCGCT 1 cut(s) 2132
Eco52I CGGCCG 1 cut(s) 1454
Eco53kI GAGCTC 1 cut(s) 1522
Eco57I CTGAAG 5 cut(s) 654, 1347, 1662, 1728, 1869
Eco81I CCTNAGG 1 cut(s) 831
Eco91I GGTNACC 1 cut(s) 817
EcoICRI GAGCTC 1 cut(s) 1522
EcoNI CCTNNNNNAGG 1 cut(s) 2278
EcoO109I RGGNCCY 1 cut(s) 827
EcoO65I GGTNACC 1 cut(s) 817
EcoRII CCWGG 1 cut(s) 1362
EcoRV GATATC 1 cut(s) 2286
EcoT38I GRGCYC 2 cut(s) 1362, 1524
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 2063, 2095
FaqI GGGAC 4 cut(s) 348, 834, 1606, 2817
FauI CCCGC 1 cut(s) 2688
FauNDI CATATG 1 cut(s) 352
FbaI TGATCA 2 cut(s) 787, 2371
Fnu4HI GCNGC 8 cut(s) 35, 333, 876, 1485, 1625, 1666, 2129, 2196
FriOI GRGCYC 2 cut(s) 1362, 1524
FseI GGCCGGCC 1 cut(s) 1456
Fsp4HI GCNGC 8 cut(s) 35, 333, 876, 1485, 1625, 1666, 2129, 2196
FspBI CTAG 3 cut(s) 1494, 2289, 2336
GlaI GCGC 2 cut(s) 738, 2132
GluI GCNGC 8 cut(s) 35, 333, 876, 1485, 1625, 1666, 2129, 2196
GsaI CCCAGC 1 cut(s) 1202
GsuI CTGGAG 2 cut(s) 47, 2450
HaeII RGCGCY 1 cut(s) 2134
HapII CCGG 2 cut(s) 1453, 2801
HgaI GACGC 1 cut(s) 2768
HhaI GCGC 2 cut(s) 739, 2133
Hin1I GRCGYC 3 cut(s) 2163, 2760, 2922
Hin6I GCGC 2 cut(s) 737, 2131
HinP1I GCGC 2 cut(s) 737, 2131
HincII GTYRAC 3 cut(s) 1600, 1993, 2650
HindII GTYRAC 3 cut(s) 1600, 1993, 2650
HindIII AAGCTT 2 cut(s) 418, 677
HinfI GANTC 9 cut(s) 800, 965, 1549, 1703, 1874, 2298, 2651, 2783, 2830
HpaI GTTAAC 1 cut(s) 1600
HpaII CCGG 2 cut(s) 1453, 2801
Hpy166II GTNNAC 4 cut(s) 1600, 1982, 1993, 2650
Hpy188III TCNNGA 6 cut(s) 458, 969, 1724, 1833, 1927, 2289
Hpy8I GTNNAC 4 cut(s) 1600, 1982, 1993, 2650
Hpy99I CGWCG 1 cut(s) 2168
HpyCH4III ACNGT 9 cut(s) 328, 348, 622, 871, 1597, 1746, 1986, 2327, 2467
HpyCH4IV ACGT 5 cut(s) 2163, 2293, 2362, 2609, 2922
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 208, 1481, 2906
HpySE526I ACGT 5 cut(s) 2163, 2293, 2362, 2609, 2922
Hsp92I GRCGYC 3 cut(s) 2163, 2760, 2922
HspAI GCGC 2 cut(s) 737, 2131
KroI GCCGGC 1 cut(s) 1452
KroNI GCCGGC 1 cut(s) 1454
Ksp22I TGATCA 2 cut(s) 787, 2371
KspAI GTTAAC 1 cut(s) 1600
LguI GCTCTTC 2 cut(s) 594, 1137
LmnI GCTCC 4 cut(s) 31, 85, 1357, 1621
Lsp1109I GCAGC 8 cut(s) 46, 344, 862, 1471, 1636, 1652, 2140, 2182
LweI GCATC 6 cut(s) 151, 417, 1329, 1742, 1862, 2666
MaeI CTAG 3 cut(s) 1494, 2289, 2336
MaeII ACGT 5 cut(s) 2163, 2293, 2362, 2609, 2922
MaeIII GTNAC 2 cut(s) 817, 1690
MbiI CCGCTC 1 cut(s) 2695
MfeI CAATTG 2 cut(s) 837, 1527
MflI RGATCY 2 cut(s) 574, 1433
MhlI GDGCHC 2 cut(s) 1362, 1524
MlsI TGGCCA 4 cut(s) 2017, 2409, 2523, 2736
MluNI TGGCCA 4 cut(s) 2017, 2409, 2523, 2736
MlyI GAGTC 6 cut(s) 1543, 1883, 2307, 2645, 2777, 2839
Mox20I TGGCCA 4 cut(s) 2017, 2409, 2523, 2736
MroNI GCCGGC 1 cut(s) 1452
MroXI GAANNNNTTC 2 cut(s) 315, 564
MscI TGGCCA 4 cut(s) 2017, 2409, 2523, 2736
MslI CAYNNNNRTG 2 cut(s) 789, 795
Msp20I TGGCCA 4 cut(s) 2017, 2409, 2523, 2736
MspA1I CMGCKG 3 cut(s) 322, 1508, 2306
MspI CCGG 2 cut(s) 1453, 2801
MspR9I CCNGG 1 cut(s) 1364
MunI CAATTG 2 cut(s) 837, 1527
Mva1269I GAATGC 2 cut(s) 1668, 2870
MvaI CCWGG 1 cut(s) 1364
MvnI CGCG 1 cut(s) 737
MwoI GCNNNNNNNGC 4 cut(s) 31, 208, 1481, 2906
NaeI GCCGGC 1 cut(s) 1454
NcoI CCATGG 3 cut(s) 522, 540, 711
NdeI CATATG 1 cut(s) 352
NgoMIV GCCGGC 1 cut(s) 1452
NlaIV GGNNCC 6 cut(s) 828, 1038, 1359, 1369, 1435, 1909
NmeAIII GCCGAG 1 cut(s) 1133
NmuCI GTSAC 2 cut(s) 817, 1690
NspI RCATGY 1 cut(s) 2264
PaeI GCATGC 1 cut(s) 2264
PasI CCCWGGG 1 cut(s) 1363
PceI AGGCCT 1 cut(s) 1958
PciSI GCTCTTC 2 cut(s) 594, 1137
PcsI WCGNNNNNNNCGW 1 cut(s) 2251
PctI GAATGC 2 cut(s) 1668, 2870
PdiI GCCGGC 1 cut(s) 1454
PdmI GAANNNNTTC 2 cut(s) 315, 564
PfeI GAWTC 3 cut(s) 800, 965, 1703
PkrI GCNGC 8 cut(s) 36, 334, 877, 1486, 1626, 1667, 2130, 2197
PleI GAGTC 6 cut(s) 1543, 1882, 2306, 2645, 2777, 2838
PpsI GAGTC 6 cut(s) 1543, 1882, 2306, 2645, 2777, 2838
PshBI ATTAAT 2 cut(s) 188, 452
Psp124BI GAGCTC 1 cut(s) 1524
Psp6I CCWGG 1 cut(s) 1362
PspEI GGTNACC 1 cut(s) 817
PspFI CCCAGC 1 cut(s) 1198
PspGI CCWGG 1 cut(s) 1362
PspN4I GGNNCC 6 cut(s) 828, 1038, 1359, 1369, 1435, 1909
PspPI GGNCC 4 cut(s) 150, 433, 827, 1450
PstI CTGCAG 1 cut(s) 922
PstNI CAGNNNCTG 2 cut(s) 1022, 2906
PsuI RGATCY 2 cut(s) 574, 1433
PvuII CAGCTG 3 cut(s) 322, 1508, 2306
RigI GGCCGGCC 1 cut(s) 1456
RsaI GTAC 4 cut(s) 1332, 1774, 1846, 2334
RsaNI GTAC 4 cut(s) 1331, 1773, 1845, 2333
RseI CAYNNNNRTG 2 cut(s) 789, 795
SacI GAGCTC 1 cut(s) 1524
SapI GCTCTTC 2 cut(s) 594, 1137
SatI GCNGC 8 cut(s) 35, 333, 876, 1485, 1625, 1666, 2129, 2196
Sau96I GGNCC 4 cut(s) 150, 433, 827, 1450
ScaI AGTACT 1 cut(s) 1846
SchI GAGTC 6 cut(s) 1543, 1883, 2307, 2645, 2777, 2839
ScrFI CCNGG 1 cut(s) 1364
SduI GDGCHC 2 cut(s) 1362, 1524
SfaNI GCATC 6 cut(s) 151, 417, 1329, 1742, 1862, 2666
SfcI CTRYAG 3 cut(s) 327, 870, 918
SinI GGWCC 2 cut(s) 150, 433
SmiMI CAYNNNNRTG 2 cut(s) 789, 795
SmlI CTYRAG 3 cut(s) 38, 458, 1925
SmoI CTYRAG 3 cut(s) 38, 458, 1925
SphI GCATGC 1 cut(s) 2264
SseBI AGGCCT 1 cut(s) 1958
SsiI CCGC 5 cut(s) 1819, 2321, 2435, 2695, 2787
SspI AATATT 1 cut(s) 55
SspMI CTAG 3 cut(s) 1494, 2289, 2336
SstI GAGCTC 1 cut(s) 1524
StuI AGGCCT 1 cut(s) 1958
StyD4I CCNGG 1 cut(s) 1362
TaaI ACNGT 9 cut(s) 328, 348, 622, 871, 1597, 1746, 1986, 2327, 2467
TaiI ACGT 5 cut(s) 2166, 2296, 2365, 2612, 2925
TaqI TCGA 2 cut(s) 1834, 2166
TaqII GACCGA 1 cut(s) 836
TatI WGTACW 2 cut(s) 1844, 2332
TfiI GAWTC 3 cut(s) 800, 965, 1703
TscAI CASTG 5 cut(s) 451, 604, 708, 724, 1783
TseFI GTSAC 2 cut(s) 817, 1690
TseI GCWGC 8 cut(s) 34, 332, 875, 1484, 1624, 1665, 2128, 2195
Tsp45I GTSAC 2 cut(s) 817, 1690
TspGWI ACGGA 3 cut(s) 1784, 2242, 2733
TspRI CASTG 5 cut(s) 451, 604, 708, 724, 1783
VpaK11BI GGWCC 2 cut(s) 150, 433
VspI ATTAAT 2 cut(s) 188, 452
XagI CCTNNNNNAGG 1 cut(s) 2278
XapI RAATTY 4 cut(s) 564, 910, 1063, 1183
XbaI TCTAGA 1 cut(s) 2288
XceI RCATGY 1 cut(s) 2264
XcmI CCANNNNNNNNNTGG 3 cut(s) 2531, 2612, 2744
XmnI GAANNNNTTC 2 cut(s) 315, 564
XspI CTAG 3 cut(s) 1494, 2289, 2336
ZraI GACGTC 2 cut(s) 2164, 2923
ZrmI AGTACT 1 cut(s) 1846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.