pycom01g06700

Magnesium-dependent phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004104
Physical Location & Seq
Forward (+)
7213986 .. 7215555
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g06700.3

Sequence Viewer

Length: 516 bp
ATGGGCGACGACGAGAAGGTGAAGAGCGAGGCTCTGCAGATAATCGAACGACACCAGAACTTGCCGAGTCTCGTTGTCTTCGATCTCGACTACACCCTCTGGCCCTTCTTCTGCGAGTGCTGCTACTACGACGACACGCCGTATCTTTACCCGCAAGCCTCGGGAATATTGTATGCGCTCAAGGACAAGGGAATATCCATGGCTGTTGCTTCGAGATCTCCAACTTCTGACGTTGCCAAATCTTTCCTCCAAACACTGGGTATCCCCTCATTGTTTCTCACCCAGGAGATTTTCTCCAGCAGGACTCACAAGACAGAGCATTTCCAAAGAATTCATGCAACCACTGGGGTACCCTTTACTGAGATGCTTTTCTTTGACGACGAGGATAGAAATATTCAAACGGTATCAAAAATGGGTGTGACAAGCATTTTAGTTGGTAATGGTGTGAACCTTGGAGCTTTGAGGCAGGGTTTGTCCGAATTTGCGCGAAAGTCAGCTTCATCGAGCAGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_PPase

Protein Analysis

172

Amino Acids

19.13

Weight (kDa)

5.52

Isoelectric Point (pI)

59.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 349
AccB1I GGYRCC 1 cut(s) 349
AccB7I CCANNNNNTGG 1 cut(s) 256
AccII CGCG 1 cut(s) 487
AciI CCGC 1 cut(s) 152
AcsI RAATTY 2 cut(s) 330, 479
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 1 cut(s) 398
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 3 cut(s) 458, 497, 513
AluI AGCT 3 cut(s) 458, 497, 513
Alw26I GTCTC 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 2 cut(s) 330, 479
Asp718I GGTACC 1 cut(s) 349
AspLEI GCGC 2 cut(s) 178, 487
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 31, 271
AvaI CYCGRG 1 cut(s) 160
BaeI ACNNNNGTAYC 2 cut(s) 333, 366
BanI GGYRCC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 107
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 284
BciVI GTATCC 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 1 cut(s) 514
BfmI CTRYAG 1 cut(s) 35
BfuI GTATCC 1 cut(s) 272
BglII AGATCT 1 cut(s) 215
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 1 cut(s) 284
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 1 cut(s) 284
BmrI ACTGGG 2 cut(s) 266, 354
BmsI GCATC 1 cut(s) 354
BmuI ACTGGG 2 cut(s) 266, 354
BpiI GAAGAC 1 cut(s) 70
BplI GAGNNNNNCTC 4 cut(s) 16, 48, 278, 310
BpmI CTGGAG 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 164
BsaJI CCNNGG 4 cut(s) 159, 198, 282, 451
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 261, 349
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 4 cut(s) 159, 198, 282, 451
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 2 cut(s) 261, 349
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 349
BsiHKCI CYCGRG 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 74
BsnI GGCC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 160
Bsp143I GATC 2 cut(s) 82, 215
Bsp19I CCATGG 1 cut(s) 198
BspACI CCGC 1 cut(s) 152
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 351
BspMAI CTGCAG 1 cut(s) 39
BspQI GCTCTTC 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 349
BsrI ACTGG 2 cut(s) 261, 349
BssECI CCNNGG 4 cut(s) 159, 198, 282, 451
BssMI GATC 2 cut(s) 82, 215
BssT1I CCWWGG 2 cut(s) 198, 451
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 360
BstDSI CCRYGG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 487
BstHHI GCGC 2 cut(s) 178, 487
BstKTI GATC 2 cut(s) 85, 218
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 2 cut(s) 82, 215
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 1 cut(s) 487
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuI GTATCC 1 cut(s) 272
BsuRI GGCC 1 cut(s) 103
BtgI CCRYGG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 254, 342
Cac8I GCNNGC 1 cut(s) 156
CfoI GCGC 2 cut(s) 178, 487
Cfr13I GGNCC 1 cut(s) 102
Csp6I GTAC 1 cut(s) 350
CviAII CATG 2 cut(s) 199, 335
CviJI RGCY 7 cut(s) 32, 103, 158, 203, 458, 497, 513
CviKI_1 RGCY 7 cut(s) 32, 103, 158, 203, 458, 497, 513
CviQI GTAC 1 cut(s) 350
DdeI CTNAG 1 cut(s) 360
DpnI GATC 2 cut(s) 84, 217
DpnII GATC 2 cut(s) 82, 215
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 198, 451
Eco88I CYCGRG 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 2 cut(s) 198, 451
ErhI CCWWGG 2 cut(s) 198, 451
FaeI CATG 2 cut(s) 202, 338
FaiI YATR 3 cut(s) 174, 200, 336
FatI CATG 2 cut(s) 198, 334
FauI CCCGC 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 1 cut(s) 514
GlaI GCGC 2 cut(s) 177, 486
GluI GCNGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 280
HaeIII GGCC 1 cut(s) 103
HhaI GCGC 2 cut(s) 178, 487
Hin1II CATG 2 cut(s) 202, 338
Hin6I GCGC 2 cut(s) 176, 485
HinP1I GCGC 2 cut(s) 176, 485
HinfI GANTC 2 cut(s) 67, 304
HphI GGTGA 2 cut(s) 31, 271
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 2 cut(s) 229, 478
Hpy188III TCNNGA 3 cut(s) 86, 162, 213
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 4 cut(s) 11, 14, 134, 383
HpyAV CCTTC 2 cut(s) 10, 115
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 2 cut(s) 37, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpyF3I CTNAG 1 cut(s) 360
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 2 cut(s) 202, 338
HspAI GCGC 2 cut(s) 176, 485
KpnI GGTACC 1 cut(s) 353
Kzo9I GATC 2 cut(s) 82, 215
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 2 cut(s) 455, 510
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 1 cut(s) 354
MaeI CTAG 1 cut(s) 514
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 418
MalI GATC 2 cut(s) 84, 217
MboI GATC 2 cut(s) 82, 215
MboII GAAGA 3 cut(s) 34, 70, 100
MflI RGATCY 1 cut(s) 215
MluCI AATT 2 cut(s) 330, 479
MlyI GAGTC 2 cut(s) 76, 298
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 7 cut(s) 22, 107, 169, 257, 277, 376, 456
MspR9I CCNGG 1 cut(s) 284
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 1 cut(s) 120
NcoI CCATGG 1 cut(s) 198
NdeII GATC 2 cut(s) 82, 215
NlaIII CATG 2 cut(s) 202, 338
NlaIV GGNNCC 1 cut(s) 351
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PflMI CCANNNNNTGG 1 cut(s) 256
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 2 cut(s) 75, 298
PpsI GAGTC 2 cut(s) 75, 298
Psp6I CCWGG 1 cut(s) 282
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 1 cut(s) 102
PstI CTGCAG 1 cut(s) 39
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 17
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 2 cut(s) 82, 215
Sau96I GGNCC 1 cut(s) 102
SchI GAGTC 2 cut(s) 76, 298
ScrFI CCNGG 1 cut(s) 284
SetI ASST 6 cut(s) 21, 234, 453, 460, 499, 515
SfaNI GCATC 1 cut(s) 354
SfcI CTRYAG 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 2 cut(s) 330, 479
SsiI CCGC 1 cut(s) 152
SspI AATATT 2 cut(s) 168, 394
SspMI CTAG 1 cut(s) 514
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 2 cut(s) 198, 451
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 1 cut(s) 234
TaqI TCGA 5 cut(s) 45, 81, 87, 212, 503
TasI AATT 2 cut(s) 330, 479
TscAI CASTG 2 cut(s) 261, 349
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 2 cut(s) 323, 489
TspRI CASTG 2 cut(s) 261, 349
Van91I CCANNNNNTGG 1 cut(s) 256
XapI RAATTY 2 cut(s) 330, 479
XspI CTAG 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.