pycom01g14420

Macrophage erythroblast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003326
Physical Location & Seq
Forward (+)
14585812 .. 14588770
2959 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 834 bp
ATGGAAATCGATTCCCTCCCCAACGGTACCGCCGCGTCGCCGGGACCGACCCTAGGACCCGTCCCCACCGCCGCAACCCCTCCTCCTTCCTCGAAACTGAGCCACCTCGCCGACTCCCTGAAGCTGGAGCACCAGTTCCTCAGAGTCCCCTTCGAGCACTACAAGAAGACGATCCGCACCAACCACCGCGTCATGGAGAAGGAGATTTCCTCCGTTATTAACGGCGTCTCCGAAGCCGCTGACCGGGACAACATGTCGTCGGACGACGCCGTGAATCATCTCAGCTCCCTCGTTTCCAGGCTGCAGGGGCTTAAACGAAAGTTGGAAGAGGGGAGTAAGACGGAGCACTTGCAGGCGCAGAGGTGCCGGGCTCGGCTTGATCATTTAGAATCGGCAGATGTGGAGAGTCTGTCTGAATGGAATGACACACGTGTGAACCGGATACTTGTGGACTACATGTTGCGGATGTCTTATTATGATACGGCAGTGAAGCTGGCAGAAAGCAGAAATATTCAGGATTTTGTTGATATTGATGTATTCCAAGAAGCAAAAAAGGTTATTGAAGCCCTTCAGAATAAGGAGGTAGGCCCTGCCCTAGCCTGGTGTGCCGAGAACAAGTCACGGTTAAAGAAATCCAAGAGTAAATTCGAGTTTCAGCTGAGACTTCAAGAGTTTATAGAGTTGGTACGAGCTGAAAAAAACTTGCGAGCGATCACATATGCTCAAAAGTACCTAGCACCATGGGGAACTACTCATATGAAAGAATTCCAGCGGGTCTTTGTAACAGTAGCTTATAAGAGTACTACAGAATGTGCTCCCAAGAGTTTTATTTGA

Protein Analysis

278

Amino Acids

31.47

Weight (kDa)

8.62

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTLH PF10607 179 - 272 6.8e-29 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
Acc65I GGTACC 1 cut(s) 26
AccB1I GGYRCC 2 cut(s) 26, 363
AccII CGCG 2 cut(s) 35, 189
AciI CCGC 9 cut(s) 30, 33, 69, 72, 175, 187, 237, 463, 772
AclWI GGATC 1 cut(s) 166
AcsI RAATTY 2 cut(s) 644, 764
AcuI CTGAAG 2 cut(s) 140, 554
AcvI CACGTG 1 cut(s) 431
AcyI GRCGYC 2 cut(s) 225, 267
AfaI GTAC 4 cut(s) 28, 687, 731, 802
AfiI CCNNNNNNNGG 5 cut(s) 53, 124, 193, 243, 600
AflIII ACRYGT 4 cut(s) 252, 428, 430, 456
AgsI TTSAA 2 cut(s) 563, 668
AhdI GACNNNNNGTC 1 cut(s) 253
AjnI CCWGG 2 cut(s) 296, 599
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AleI CACNNNNGTG 1 cut(s) 431
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 6 cut(s) 124, 285, 493, 658, 692, 791
AluI AGCT 6 cut(s) 124, 285, 493, 658, 692, 791
Alw21I GWGCWC 4 cut(s) 132, 159, 348, 817
Alw26I GTCTC 2 cut(s) 232, 655
AlwI GGATC 1 cut(s) 166
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 1 cut(s) 301
ApoI RAATTY 2 cut(s) 644, 764
Asp700I GAANNNNTTC 2 cut(s) 567, 764
Asp718I GGTACC 1 cut(s) 26
AspA2I CCTAGG 1 cut(s) 52
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 3 cut(s) 44, 56, 587
AsuC2I CCSGG 3 cut(s) 42, 245, 368
AvaII GGWCC 2 cut(s) 44, 56
AvrII CCTAGG 1 cut(s) 52
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BanI GGYRCC 2 cut(s) 26, 363
BanII GRGCYC 1 cut(s) 373
BbrPI CACGTG 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 4 cut(s) 132, 159, 348, 817
BbvI GCAGC 1 cut(s) 288
BceAI ACGGC 3 cut(s) 238, 254, 498
BciT130I CCWGG 2 cut(s) 298, 601
BciVI GTATCC 1 cut(s) 435
BclI TGATCA 1 cut(s) 379
BcnI CCSGG 3 cut(s) 42, 245, 368
BcoDI GTCTC 2 cut(s) 232, 655
BfaI CTAG 3 cut(s) 53, 596, 734
BfmI CTRYAG 2 cut(s) 302, 804
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 4 cut(s) 33, 72, 237, 302
BlnI CCTAGG 1 cut(s) 52
BlsI GCNGC 4 cut(s) 34, 73, 238, 303
BmcAI AGTACT 1 cut(s) 802
Bme1390I CCNGG 5 cut(s) 42, 245, 298, 368, 601
Bme18I GGWCC 2 cut(s) 44, 56
BmeRI GACNNNNNGTC 1 cut(s) 253
BmgT120I GGNCC 3 cut(s) 44, 56, 587
BmiI GGNNCC 4 cut(s) 28, 45, 58, 365
BmrFI CCNGG 5 cut(s) 42, 245, 298, 368, 601
BpiI GAAGAC 1 cut(s) 173
BplI GAGNNNNNCTC 2 cut(s) 194, 226
BpmI CTGGAG 1 cut(s) 146
BpuMI CCSGG 3 cut(s) 42, 245, 368
Bsa29I ATCGAT 1 cut(s) 9
BsaAI YACGTR 1 cut(s) 431
BsaHI GRCGYC 2 cut(s) 225, 267
BsaJI CCNNGG 2 cut(s) 52, 740
BsaWI WCCGGW 1 cut(s) 438
BsaXI ACNNNNNCTCC 8 cut(s) 67, 97, 119, 149, 212, 242, 395, 425
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 124, 193, 243, 600
Bse1I ACTGG 1 cut(s) 133
BseBI CCWGG 2 cut(s) 298, 601
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 52, 740
BseGI GGATG 1 cut(s) 471
BseLI CCNNNNNNNGG 5 cut(s) 53, 124, 193, 243, 600
BseMII CTCAG 4 cut(s) 89, 154, 295, 650
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 1 cut(s) 72
BseXI GCAGC 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 35, 189
BshFI GGCC 1 cut(s) 588
BshNI GGYRCC 2 cut(s) 26, 363
BshVI ATCGAT 1 cut(s) 9
BsiHKAI GWGCWC 4 cut(s) 132, 159, 348, 817
BsiSI CCGG 4 cut(s) 41, 244, 367, 439
BslFI GGGAC 4 cut(s) 47, 57, 131, 260
BslI CCNNNNNNNGG 5 cut(s) 53, 124, 193, 243, 600
BsmAI GTCTC 2 cut(s) 232, 655
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 4 cut(s) 47, 57, 131, 260
BsnI GGCC 1 cut(s) 588
Bsp1286I GDGCHC 5 cut(s) 132, 159, 348, 373, 817
Bsp143I GATC 3 cut(s) 171, 379, 711
Bsp19I CCATGG 1 cut(s) 740
BspACI CCGC 9 cut(s) 30, 33, 69, 72, 175, 187, 237, 463, 772
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 4 cut(s) 90, 153, 294, 651
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 2 cut(s) 35, 189
BspLI GGNNCC 4 cut(s) 28, 45, 58, 365
BspMAI CTGCAG 1 cut(s) 306
BspPI GGATC 1 cut(s) 166
BspT107I GGYRCC 2 cut(s) 26, 363
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 52, 740
BssMI GATC 3 cut(s) 171, 379, 711
BssNI GRCGYC 2 cut(s) 225, 267
BssT1I CCWWGG 2 cut(s) 52, 740
Bst2UI CCWGG 2 cut(s) 298, 601
Bst4CI ACNGT 3 cut(s) 26, 624, 787
Bst6I CTCTTC 1 cut(s) 321
BstACI GRCGYC 2 cut(s) 225, 267
BstBAI YACGTR 1 cut(s) 431
BstC8I GCNNGC 3 cut(s) 354, 495, 708
BstDEI CTNAG 4 cut(s) 98, 140, 281, 659
BstDSI CCRYGG 1 cut(s) 740
BstF5I GGATG 1 cut(s) 471
BstFNI CGCG 2 cut(s) 35, 189
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 3 cut(s) 174, 382, 714
BstMAI GTCTC 2 cut(s) 232, 655
BstMBI GATC 3 cut(s) 171, 379, 711
BstMWI GCNNNNNNNGC 2 cut(s) 307, 605
BstNI CCWGG 2 cut(s) 298, 601
BstNSI RCATGY 2 cut(s) 256, 460
BstSCI CCNGG 5 cut(s) 40, 243, 296, 366, 599
BstSFI CTRYAG 2 cut(s) 302, 804
BstUI CGCG 2 cut(s) 35, 189
BstV1I GCAGC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 173
Bsu15I ATCGAT 1 cut(s) 9
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 588
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 740
BtsCI GGATG 1 cut(s) 471
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 3 cut(s) 354, 495, 708
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 3 cut(s) 44, 56, 587
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 4 cut(s) 24, 178, 214, 275
Csp6I GTAC 4 cut(s) 27, 686, 730, 801
CviAII CATG 4 cut(s) 193, 253, 457, 741
CviQI GTAC 4 cut(s) 27, 686, 730, 801
DdeI CTNAG 4 cut(s) 98, 140, 281, 659
DpnI GATC 3 cut(s) 173, 381, 713
DpnII GATC 3 cut(s) 171, 379, 711
DriI GACNNNNNGTC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 321
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 52, 740
Eco24I GRGCYC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 44, 56
Eco57I CTGAAG 2 cut(s) 140, 554
Eco72I CACGTG 1 cut(s) 431
EcoO109I RGGNCCY 2 cut(s) 56, 587
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 296, 599
EcoT14I CCWWGG 2 cut(s) 52, 740
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 52, 740
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 4 cut(s) 196, 256, 460, 744
FaqI GGGAC 4 cut(s) 47, 57, 131, 260
FatI CATG 4 cut(s) 192, 252, 456, 740
FauI CCCGC 1 cut(s) 765
FauNDI CATATG 2 cut(s) 718, 756
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 4 cut(s) 33, 72, 237, 302
FokI GGATG 1 cut(s) 478
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 4 cut(s) 33, 72, 237, 302
FspBI CTAG 3 cut(s) 53, 596, 734
GlaI GCGC 1 cut(s) 357
GluI GCNGC 4 cut(s) 33, 72, 237, 302
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 1 cut(s) 588
HapII CCGG 4 cut(s) 41, 244, 367, 439
HgaI GACGC 4 cut(s) 24, 178, 214, 275
HhaI GCGC 1 cut(s) 358
Hin1I GRCGYC 2 cut(s) 225, 267
Hin1II CATG 4 cut(s) 196, 256, 460, 744
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 6 cut(s) 11, 113, 144, 274, 389, 406
HpaII CCGG 4 cut(s) 41, 244, 367, 439
Hpy166II GTNNAC 2 cut(s) 436, 451
Hpy188I TCNGA 5 cut(s) 143, 232, 262, 415, 573
Hpy188III TCNNGA 2 cut(s) 515, 668
Hpy8I GTNNAC 2 cut(s) 436, 451
Hpy99I CGWCG 3 cut(s) 40, 262, 269
HpyAV CCTTC 4 cut(s) 96, 160, 193, 578
HpyCH4III ACNGT 3 cut(s) 26, 624, 787
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 2 cut(s) 304, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 605
HpyF3I CTNAG 4 cut(s) 98, 140, 281, 659
HpySE526I ACGT 1 cut(s) 430
Hsp92I GRCGYC 2 cut(s) 225, 267
Hsp92II CATG 4 cut(s) 196, 256, 460, 744
HspAI GCGC 1 cut(s) 356
KpnI GGTACC 1 cut(s) 30
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 3 cut(s) 171, 379, 711
LmnI GCTCC 4 cut(s) 127, 290, 343, 820
Lsp1109I GCAGC 1 cut(s) 288
MaeI CTAG 3 cut(s) 53, 596, 734
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 2 cut(s) 618, 781
MalI GATC 3 cut(s) 173, 381, 713
MboI GATC 3 cut(s) 171, 379, 711
MboII GAAGA 2 cut(s) 178, 338
MhlI GDGCHC 5 cut(s) 132, 159, 348, 373, 817
MluCI AATT 2 cut(s) 644, 764
MlyI GAGTC 3 cut(s) 107, 153, 415
MmeI TCCRAC 2 cut(s) 240, 303
MroXI GAANNNNTTC 2 cut(s) 567, 764
MseI TTAA 3 cut(s) 219, 312, 626
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 3 cut(s) 239, 658, 772
MspI CCGG 4 cut(s) 41, 244, 367, 439
MspR9I CCNGG 5 cut(s) 42, 245, 298, 368, 601
MvaI CCWGG 2 cut(s) 298, 601
MvnI CGCG 2 cut(s) 35, 189
MwoI GCNNNNNNNGC 2 cut(s) 307, 605
NciI CCSGG 3 cut(s) 42, 245, 368
NcoI CCATGG 1 cut(s) 740
NdeI CATATG 2 cut(s) 718, 756
NdeII GATC 3 cut(s) 171, 379, 711
NlaIII CATG 4 cut(s) 196, 256, 460, 744
NlaIV GGNNCC 4 cut(s) 28, 45, 58, 365
NmeAIII GCCGAG 2 cut(s) 352, 634
NmuCI GTSAC 1 cut(s) 618
NspI RCATGY 2 cut(s) 256, 460
OliI CACNNNNGTG 1 cut(s) 431
PciI ACATGT 2 cut(s) 252, 456
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 228
PdmI GAANNNNTTC 2 cut(s) 567, 764
PfeI GAWTC 3 cut(s) 11, 274, 389
PkrI GCNGC 4 cut(s) 34, 73, 238, 303
PleI GAGTC 3 cut(s) 107, 152, 414
PmaCI CACGTG 1 cut(s) 431
PmlI CACGTG 1 cut(s) 431
PpsI GAGTC 3 cut(s) 107, 152, 414
Ppu21I YACGTR 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 56
PscI ACATGT 2 cut(s) 252, 456
PsiI TTATAA 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 296, 599
PspCI CACGTG 1 cut(s) 431
PspGI CCWGG 2 cut(s) 296, 599
PspN4I GGNNCC 4 cut(s) 28, 45, 58, 365
PspPI GGNCC 3 cut(s) 44, 56, 587
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 306
PvuII CAGCTG 1 cut(s) 658
RsaI GTAC 4 cut(s) 28, 687, 731, 802
RsaNI GTAC 4 cut(s) 27, 686, 730, 801
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 3 cut(s) 219, 312, 626
SatI GCNGC 4 cut(s) 33, 72, 237, 302
Sau3AI GATC 3 cut(s) 171, 379, 711
Sau96I GGNCC 3 cut(s) 44, 56, 587
ScaI AGTACT 1 cut(s) 802
SchI GAGTC 3 cut(s) 107, 153, 415
ScrFI CCNGG 5 cut(s) 42, 245, 298, 368, 601
SduI GDGCHC 5 cut(s) 132, 159, 348, 373, 817
SfcI CTRYAG 2 cut(s) 302, 804
SinI GGWCC 2 cut(s) 44, 56
SmiMI CAYNNNNRTG 1 cut(s) 431
Sse9I AATT 2 cut(s) 644, 764
SsiI CCGC 9 cut(s) 30, 33, 69, 72, 175, 187, 237, 463, 772
SspI AATATT 1 cut(s) 511
SspMI CTAG 3 cut(s) 53, 596, 734
StyD4I CCNGG 5 cut(s) 40, 243, 296, 366, 599
StyI CCWWGG 2 cut(s) 52, 740
TaaI ACNGT 3 cut(s) 26, 624, 787
TaiI ACGT 1 cut(s) 433
TaqI TCGA 4 cut(s) 9, 92, 153, 648
TaqII GACCGA 1 cut(s) 61
TasI AATT 2 cut(s) 644, 764
TatI WGTACW 1 cut(s) 800
TauI GCSGC 3 cut(s) 35, 74, 239
TfiI GAWTC 3 cut(s) 11, 274, 389
Tru1I TTAA 3 cut(s) 219, 312, 626
Tru9I TTAA 3 cut(s) 219, 312, 626
TscAI CASTG 1 cut(s) 492
TseFI GTSAC 1 cut(s) 618
TseI GCWGC 1 cut(s) 301
Tsp45I GTSAC 1 cut(s) 618
TspDTI ATGAA 1 cut(s) 773
TspGWI ACGGA 2 cut(s) 202, 356
TspRI CASTG 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 44, 56
XapI RAATTY 2 cut(s) 644, 764
XceI RCATGY 2 cut(s) 256, 460
XmaJI CCTAGG 1 cut(s) 52
XmnI GAANNNNTTC 2 cut(s) 567, 764
XspI CTAG 3 cut(s) 53, 596, 734
ZrmI AGTACT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.