Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0002550

Physical Location & Seq

Genomic Coordinates Forward (+)

21224364 .. 21230925

Gene Structure 6562 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3189 bp

ATGATGAACTTTTCAAGGAGCCGATCGATCACCCAGTTGCTTACTCTTGATAACTTTAGGCAAAATGAGCAGCTTTCCAAGTATGGGGACACAAAATCGGCTCGGAATATCATGCTTATAGAACTTAAAAAGCTCATTGAGGCGAACCCATTATTTCGTGATAAGCTTGCATTCCCAGCCTTCAAAAGTTCACGTCTTCGAACGTTAATAAACCAGAGTCTCAATTGGCAGCACCAGCTTTGCAAGAATCCCCGTCCAAACCCTGATATTAAAACACTATTTATGGATCATTCTTGCACTCCAACTGCTAACGGATCTCGTCCACCACCCACAAATAGTCCCCTTGCAGGACCAATTCCAAAGGCTGGCGCATTTCCTCCTATTGGTGTTCCTCCTATTGGTCCATTTCAACCCGTTGTCTCCCCATCTCCTGGTGCCATTTCTGGGTGGATGTCAAGCCCTAATCCTTCTTTGCCCCACCCTGCTGTTCCAGCAGCTCCTCCTGGTATGCAGCCTTCTAGTGCAGCTGCTTTCTTGAAGCACCCAAGGACCCCTACAGGTGTTACAGGGATTGATTATCAATCAGCTGATTCAGAGCACTTGATGAAGCGCATTCGCACTGGCCAAGCTGATGAGGTGTCCTTTTCTGGTGTGATGCATAATTCCAATGTCTATTCGCAAGATGATCTTCCTAAGGCTGTTGTCCGTACCCTTAGTCAAGGATCTAATGTGATGAGCATGGACTTCCATCCACAACAGCAAACTATTCTTCTAGTCGGAACAAATGTTGGTGATATCAGCCTCTGGGAAGTAGGGTCCCGGGAGAGGTTGGTACATAAGCCTTTCAAGGTTTGGGATATACAAACTGCATCAACACCGTTGCAGACAGCTCTGGTGAATGATGCTGCAATATCAGTGAATCGTTGTGTATGGGCACCAGACGGACTTATGTTGGGTGTTGCATTCTCTAAGCACATTGTTCAGATATATAATTACAATCCAACTGGAGAACTGAGACAACATTTGGAGATTGATGCTCATATCGGCGGTGTTAATGACATTGCATTCGCTCATCCCAACAAGCAACTGTGTATTGTCACGTGTGGAGATGATAGAGTGATTAAGGTATGGGATGCTGTAGCCGGACGCAGACAGTATACATTTGAAGGCCATGAAGCTCCTGTGTATTCAGTGTGCCCTCACTACAAAGAAAATATCCAGTTCATTTTTTCAACTGCCATTGACGGGAAGATTAAAGCTTGGCTATATGATTGCTTGGGATCACGAGTGGACTATGATGCTCCTGGACTTTGGTGCACTATGATGTCATATAGTGCAGATGGAACCAGACTCTTTTCCTGTGGAACAAGTAAAGAAGGTGAATCCTATCTGGTGGAGTGGAATGAGAGTGAAGGGGCTATCAAACGGACATATACCGGTTTTAGAAAACGCTGTTTAGATGTTGTTCAGTTTGACACAACAAGGAACCGATTCTTAGCTGCTGGTGATGAATTTCAAATTAAGTTCTGGGATATGGACAATACCAATGTGTTGGCAACTGTGGATGCAGATGGCGGTTTGCCTGCTAGTCCTAGGTTGAGATTCAATAAAGAAGGGTCACTGTTGGCTGTAACAACGAATGACAGTGGTATTAAGATTTTAGCAAATAATGATGGCTTGCGCTTGGTTAGAATGTTAGAGGGAAGGGCTATGGAGAAAAATCGAGGCACTTCTGAGCCCATCAACTCTAAGCCTCTGATGGTTAATGCAATAGGCGCCATTGTGAATGTTCCCAACACTGTTGTTCCATCCCTGGAACGCCCTGATAGAATACAACCAGTTGTGTCTATCAGTAATCTAGGTACTATGGAGAACAGCAGGCTTGTTGATGTTAAACCACGGATTTCTGATGATATAGACAAGATTAAGAGCTGGAAATTTTCTGATGTTGCGGATCCCTCTCAAATGAAAGCTCTGCGACTGCCCGACTCTACAACTGCTGGAAAGATTGTGAGATTAATGTACACCAACAATGGCCTAGCTCTGTTAGCCCTTGCCTCCAATGCTGTTCACAAGCTCTGGAAATGGCAGCGCAATGAAAGGAATCCATCCGGGAAGGCTAGTACATATGTTGCACCTCAGTTGTGGCAGCCACCCAATGGAACTCTTATGACTAATGATGTAAATGACAATAAACCAGCTGAGGAATCTGTTGCATGCATTGCTTTATCCAAAAATGATTCTTACGTCATGTCTGCATCTGGTGGGAAGGTCTCTTTGTTCAACATGATGACATTTAAGGTCATGACAACATTTGTATCTCCCCCTCCCGCTGCCACTTTTTTGGCATTTCATCCTCAAGACAATAATATAATTGCAATTGGCATGGAGGATTCTACTATATTAATATTTAATGTTAGGGTTGATGAGGTCAAAACCAAACTGAAAGGTCACCAGAATCGGATTACAGGCCTCGCATTTTCCCAAAATCTTAATGTGCTGGTATCTTCAGGTGCTGATACCCAGTTATGTGTGTGGAGCATTGATGGATGGGAGAAAAAGAAAACAAGGTTCATACAAGCACCAGCTGGTCGTCAACCCCCGATGGTTGGAGAAACAAAAGTTCAGTTTCAGAACGATCACAAACACCTGTTGGTTGCCCATGACAGCCAAATTGCTGTGTACGATTGCAAGCTTGAATGTTTGCGCTCATGGTCTCCAAACGATGCACTCGCTGCTCCCATATCAAGTGCAATATATTCATGTGATGGTCTACTGGTTTATGCTTCATTTTGTGACGGTGCTGTTGGAGTTTTTGATGCTGATACCTTAAGACTCAGATGTCGAGTAGCACCTACTGCTTATATACCATCATTTTCTCTCAGCGGAAACCCCAGCTATCCTTTGGTTATAGCAGCTCATCCATCTGAGCCGAATCAGATTGCACTTGGCATGACTGACGGCTCAGTGCATGTAGTTGAACCGTCTGATGTAGAGCTGAGGTGGGGTGGGGCGCCCCCTCAAGAGAATGGCCCTTCCAATTCGTTAAATCCTTCTCCTAGCGGTCAAGCATCTGAACATCCTTCCCGGACTAAGAGTAGACAGAAGAAACTTCGTGGTGCATTTTCAGGGATTTTAGGTAGTATCTTTTCCAATTTTTTTGTTAATTCGAGCCATAGCTTTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1063

Amino Acids

116.41

Weight (kDa)

8.63

Isoelectric Point (pI)

42.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR1-like_2nd	PF21889	8 - 56	7.2e-17	TPR1-like, CTLH-containing domain
zf_topless	PF21359	59 - 99	1.5e-25	TOPLESS, zinc finger domain
WD40_CDC20-Fz	PF24807	222 - 510	1.7e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	283 - 459	4.7e-14	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	303 - 421	1.1e-06	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	305 - 564	2.5e-21	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	306 - 518	7e-19	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	311 - 457	8e-06	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	320 - 422	7.4e-09	WDHD1 first WD40 domain
WD40_Prp19	PF24814	348 - 421	2.5e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	466 - 569	6.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	714 - 969	1.5e-14	THOC3 beta-propeller domain
WD40_Prp19	PF24814	715 - 898	2.9e-15	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	728 - 853	2.1e-14	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	729 - 944	5.7e-19	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	731 - 849	1.5e-11	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	732 - 850	3.8e-18	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	733 - 854	6.7e-11	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	733 - 849	5.9e-07	WDR55
Beta-prop_WDR5	PF25175	735 - 950	3e-18	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	738 - 868	1.7e-13	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	751 - 849	8.9e-11	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	751 - 850	8.9e-12	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	756 - 854	1.4e-09	WDR90/POC16, second beta-propeller
Beta-prop_RIG_1st	PF23770	765 - 829	2.7e-07	RIG first beta-propeller
WD40_Prp19	PF24814	776 - 948	1.1e-14	Prp19 WD40 domain
WD40	PF00400	812 - 847	7.3e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002550)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770 FvH4_7g33770
malus_domestica	MD01G1234100.v1.1 MD07G1306300.v1.1
prunus_persica	Prupe.2G324300_v2.0.a1
pyrus_communis	pycom01g24260 pycom07g27930
rosa_chinensis	RchiOBHm_Chr1g0382471 RchiOBHm_Chr1g0382481 RchiOBHm_Chr1g0383911
rosa_laevigata	RLG00000026087 RLG00000026174 RLG00000026177
rosa_multiflora	Rmu_sc0000110.1_g000051 Rmu_sc0003885.1_g000009 Rmu_sc0005139.1_g000028
rosa_roxburghii	Rroxscaffold_4G00277160 Rroxscaffold_4G00278020 Rroxscaffold_4G00278080
rosa_rugosa	Rorug01G0434600 Rorug01G0434900 Rorug01G0443000
rosa_samantha	Rh1AG459200 Rh1AG459300 Rh1AG459600 Rh1AG468900 Rh1BG415700 Rh1BG426600 Rh1CG429700 Rh1CG429800 Rh1CG439400 Rh1DG447800 Rh1DG447900 Rh1DG459400
rosa_wichuraiana	Rw1G040350 Rw1G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2841
AccB1I	GGYRCC	4 cut(s)	434, 934, 1775, 3013
AccB7I	CCANNNNNTGG	3 cut(s)	365, 431, 2159
AccI	GTMKAC	3 cut(s)	1157, 2773, 3100
AciI	CCGC	6 cut(s)	1047, 1575, 1952, 2331, 2886, 3063
AclI	AACGTT	1 cut(s)	203
AclWI	GGATC	6 cut(s)	294, 322, 730, 1288, 1949, 1962
AcoI	YGGCCR	1 cut(s)	622
AcsI	RAATTY	2 cut(s)	1511, 1937
AcuI	CTGAAG	1 cut(s)	2493
AcvI	CACGTG	1 cut(s)	1101
AcyI	GRCGYC	2 cut(s)	1776, 3014
AfaI	GTAC	6 cut(s)	709, 834, 1864, 2024, 2125, 2684
AflII	CTTAAG	1 cut(s)	2830
AflIII	ACRYGT	1 cut(s)	1100
AgeI	ACCGGT	1 cut(s)	1436
AjiI	CACGTC	1 cut(s)	194
AjnI	CCWGG	4 cut(s)	430, 502, 1303, 1812
Alw21I	GWGCWC	2 cut(s)	600, 1319
Alw26I	GTCTC	5 cut(s)	224, 424, 1009, 2278, 2721
Alw44I	GTGCAC	1 cut(s)	1315
AlwI	GGATC	6 cut(s)	294, 322, 730, 1288, 1949, 1962
AlwNI	CAGNNNCTG	2 cut(s)	804, 2516
Ama87I	CYCGRG	1 cut(s)	819
AoxI	GGCC	5 cut(s)	622, 1168, 2035, 2470, 3031
ApaLI	GTGCAC	1 cut(s)	1315
ApoI	RAATTY	2 cut(s)	1511, 1937
ArsI	GACNNNNNNTTYG	2 cut(s)	1049, 1081
AseI	ATTAAT	2 cut(s)	2018, 2405
AsiGI	ACCGGT	1 cut(s)	1436
Asp700I	GAANNNNTTC	1 cut(s)	1490
AspA2I	CCTAGG	1 cut(s)	1592
AspLEI	GCGC	7 cut(s)	371, 612, 1683, 1778, 2094, 2709, 3016
AspS9I	GGNCC	5 cut(s)	350, 401, 549, 816, 3032
AsuC2I	CCSGG	4 cut(s)	820, 821, 2113, 3088
AsuHPI	GGTGA	6 cut(s)	22, 803, 907, 1391, 1517, 2444
AsuII	TTCGAA	1 cut(s)	199
AvaI	CYCGRG	1 cut(s)	819
AvaII	GGWCC	4 cut(s)	350, 401, 549, 816
AvrII	CCTAGG	1 cut(s)	1592
AxyI	CCTNAGG	1 cut(s)	693
BaeGI	GKGCMC	3 cut(s)	937, 1199, 1319
BaeI	ACNNNNGTAYC	2 cut(s)	2301, 2334
BalI	TGGCCA	1 cut(s)	624
BamHI	GGATCC	1 cut(s)	1954
BanI	GGYRCC	4 cut(s)	434, 934, 1775, 3013
BanII	GRGCYC	1 cut(s)	1740
BarI	GAAGNNNNNNTAC	2 cut(s)	499, 531
BauI	CACGAG	1 cut(s)	1284
BbrPI	CACGTG	1 cut(s)	1101
BbsI	GAAGAC	1 cut(s)	188
Bbv12I	GWGCWC	2 cut(s)	600, 1319
BbvCI	CCTCAGC	2 cut(s)	2202, 2999
BceAI	ACGGC	1 cut(s)	2977
BciT130I	CCWGG	4 cut(s)	432, 504, 1305, 1814
BcnI	CCSGG	4 cut(s)	820, 821, 2113, 3088
BcoDI	GTCTC	5 cut(s)	224, 424, 1009, 2278, 2721
BfaI	CTAG	8 cut(s)	519, 773, 1587, 1593, 1859, 2039, 2121, 3060
BfmI	CTRYAG	2 cut(s)	555, 1137
BfoI	RGCGCY	2 cut(s)	1779, 3017
BfrI	CTTAAG	1 cut(s)	2830
BlnI	CCTAGG	1 cut(s)	1592
Bme1390I	CCNGG	8 cut(s)	432, 504, 820, 821, 1305, 1814, 2113, 3088
Bme18I	GGWCC	4 cut(s)	350, 401, 549, 816
BmeT110I	CYCGRG	1 cut(s)	819
BmgBI	CACGTC	1 cut(s)	194
BmgT120I	GGNCC	5 cut(s)	350, 401, 549, 816, 3032
BmrFI	CCNGG	8 cut(s)	432, 504, 820, 821, 1305, 1814, 2113, 3088
BmrI	ACTGGG	2 cut(s)	28, 2518
BmuI	ACTGGG	2 cut(s)	28, 2518
BpiI	GAAGAC	1 cut(s)	188
BpmI	CTGGAG	1 cut(s)	1026
Bpu10I	CCTNAGC	2 cut(s)	2202, 2999
Bpu14I	TTCGAA	1 cut(s)	199
BpuEI	CTTGAG	2 cut(s)	2343, 3006
BpuMI	CCSGG	4 cut(s)	820, 821, 2113, 3088
Bsa29I	ATCGAT	1 cut(s)	26
BsaAI	YACGTR	1 cut(s)	1101
BsaHI	GRCGYC	2 cut(s)	1776, 3014
BsaI	GGTCTC	2 cut(s)	2278, 2721
BsaJI	CCNNGG	5 cut(s)	545, 819, 1592, 1812, 1898
BsaWI	WCCGGW	1 cut(s)	1436
Bse118I	RCCGGY	1 cut(s)	1436
Bse1I	ACTGG	7 cut(s)	34, 625, 1009, 1219, 1838, 2524, 2781
Bse21I	CCTNAGG	1 cut(s)	693
Bse3DI	GCAATG	3 cut(s)	1059, 2101, 2220
BseBI	CCWGG	4 cut(s)	432, 504, 1305, 1814
BseCI	ATCGAT	1 cut(s)	26
BseDI	CCNNGG	5 cut(s)	545, 819, 1592, 1812, 1898
BseMI	GCAATG	3 cut(s)	1059, 2101, 2220
BseMII	CTCAG	9 cut(s)	1004, 1725, 2153, 2193, 2851, 2896, 2919, 2979, 2990
BseNI	ACTGG	7 cut(s)	34, 625, 1009, 1219, 1838, 2524, 2781
BseRI	GAGGAG	1 cut(s)	489
BseSI	GKGCMC	3 cut(s)	937, 1199, 1319
BseYI	CCCAGC	2 cut(s)	175, 2894
BsgI	GTGCAG	2 cut(s)	543, 1356
Bsh1285I	CGRYCG	1 cut(s)	26
BshFI	GGCC	5 cut(s)	624, 1170, 2037, 2472, 3033
BshNI	GGYRCC	4 cut(s)	434, 934, 1775, 3013
BshTI	ACCGGT	1 cut(s)	1436
BshVI	ATCGAT	1 cut(s)	26
BsiEI	CGRYCG	1 cut(s)	26
BsiHKAI	GWGCWC	2 cut(s)	600, 1319
BsiHKCI	CYCGRG	1 cut(s)	819
BsiSI	CCGG	5 cut(s)	820, 1143, 1437, 2112, 3088
BslFI	GGGAC	3 cut(s)	101, 324, 802
BsmAI	GTCTC	5 cut(s)	224, 424, 1009, 2278, 2721
BsmFI	GGGAC	3 cut(s)	101, 324, 802
BsmI	GAATGC	4 cut(s)	170, 612, 962, 1064
BsnI	GGCC	5 cut(s)	624, 1170, 2037, 2472, 3033
Bso31I	GGTCTC	2 cut(s)	2278, 2721
BsoBI	CYCGRG	1 cut(s)	819
Bsp119I	TTCGAA	1 cut(s)	199
Bsp1286I	GDGCHC	5 cut(s)	600, 937, 1199, 1319, 1740
Bsp1407I	TGTACA	1 cut(s)	2022
Bsp143I	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
BspACI	CCGC	6 cut(s)	1047, 1575, 1952, 2331, 2886, 3063
BspANI	GGCC	5 cut(s)	624, 1170, 2037, 2472, 3033
BspCNI	CTCAG	9 cut(s)	1005, 1726, 2152, 2194, 2850, 2895, 2920, 2978, 2991
BspDI	ATCGAT	1 cut(s)	26
BspHI	TCATGA	1 cut(s)	2304
BspPI	GGATC	6 cut(s)	294, 322, 730, 1288, 1949, 1962
BspT104I	TTCGAA	1 cut(s)	199
BspT107I	GGYRCC	4 cut(s)	434, 934, 1775, 3013
BspTI	CTTAAG	1 cut(s)	2830
BspTNI	GGTCTC	2 cut(s)	2278, 2721
BsrDI	GCAATG	3 cut(s)	1059, 2101, 2220
BsrFI	RCCGGY	1 cut(s)	1436
BsrGI	TGTACA	1 cut(s)	2022
BsrI	ACTGG	7 cut(s)	34, 625, 1009, 1219, 1838, 2524, 2781
BssAI	RCCGGY	1 cut(s)	1436
BssECI	CCNNGG	5 cut(s)	545, 819, 1592, 1812, 1898
BssMI	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
BssNAI	GTATAC	1 cut(s)	1158
BssNI	GRCGYC	2 cut(s)	1776, 3014
BssSI	CACGAG	1 cut(s)	1284
BssT1I	CCWWGG	2 cut(s)	545, 1592
Bst1107I	GTATAC	1 cut(s)	1158
Bst2BI	CACGAG	1 cut(s)	1284
Bst2UI	CCWGG	4 cut(s)	432, 504, 1305, 1814
Bst4CI	ACNGT	9 cut(s)	879, 1089, 1155, 1561, 1623, 1646, 1801, 2801, 2985
BstACI	GRCGYC	2 cut(s)	1776, 3014
BstAFI	CTTAAG	1 cut(s)	2830
BstAPI	GCANNNNNTGC	3 cut(s)	2222, 2735, 2858
BstAUI	TGTACA	1 cut(s)	2022
BstBAI	YACGTR	1 cut(s)	1101
BstBI	TTCGAA	1 cut(s)	199
BstC8I	GCNNGC	7 cut(s)	168, 367, 1584, 1679, 1880, 2218, 2693
BstDSI	CCRYGG	1 cut(s)	1898
BstEII	GGTNACC	1 cut(s)	2450
BstH2I	RGCGCY	2 cut(s)	1779, 3017
BstHHI	GCGC	7 cut(s)	371, 612, 1683, 1778, 2094, 2709, 3016
BstKTI	GATC	9 cut(s)	26, 30, 289, 317, 688, 725, 1283, 1957, 2641
BstMAI	GTCTC	5 cut(s)	224, 424, 1009, 2278, 2721
BstMBI	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
BstMCI	CGRYCG	1 cut(s)	26
BstNI	CCWGG	4 cut(s)	432, 504, 1305, 1814
BstNSI	RCATGY	2 cut(s)	2220, 2975
BstPI	GGTNACC	1 cut(s)	2450
BstSCI	CCNGG	8 cut(s)	430, 502, 818, 819, 1303, 1812, 2111, 3086
BstSFI	CTRYAG	2 cut(s)	555, 1137
BstSLI	GKGCMC	3 cut(s)	937, 1199, 1319
BstV2I	GAAGAC	1 cut(s)	188
BstX2I	RGATCY	3 cut(s)	314, 722, 1954
BstXI	CCANNNNNNTGG	3 cut(s)	1552, 2344, 3182
BstYI	RGATCY	3 cut(s)	314, 722, 1954
BstZ17I	GTATAC	1 cut(s)	1158
Bsu15I	ATCGAT	1 cut(s)	26
Bsu36I	CCTNAGG	1 cut(s)	693
BsuRI	GGCC	5 cut(s)	624, 1170, 2037, 2472, 3033
BsuTUI	ATCGAT	1 cut(s)	26
BtgI	CCRYGG	1 cut(s)	1898
BtrI	CACGTC	1 cut(s)	194
BtsIMutI	CAGTG	7 cut(s)	618, 921, 1197, 1619, 1651, 1797, 2973
Cac8I	GCNNGC	7 cut(s)	168, 367, 1584, 1679, 1880, 2218, 2693
CaiI	CAGNNNCTG	2 cut(s)	804, 2516
CciI	TCATGA	1 cut(s)	2304
CfoI	GCGC	7 cut(s)	371, 612, 1683, 1778, 2094, 2709, 3016
Cfr10I	RCCGGY	1 cut(s)	1436
Cfr13I	GGNCC	5 cut(s)	350, 401, 549, 816, 3032
Cfr9I	CCCGGG	1 cut(s)	819
ClaI	ATCGAT	1 cut(s)	26
CseI	GACGC	1 cut(s)	1155
Csp6I	GTAC	6 cut(s)	708, 833, 1863, 2023, 2124, 2683
CspAI	ACCGGT	1 cut(s)	1436
CviQI	GTAC	6 cut(s)	708, 833, 1863, 2023, 2124, 2683
DinI	GGCGCC	2 cut(s)	1777, 3015
DpnI	GATC	9 cut(s)	25, 29, 288, 316, 687, 724, 1282, 1956, 2640
DpnII	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
DrdI	GACNNNNNNGTC	1 cut(s)	2841
DseDI	GACNNNNNNGTC	1 cut(s)	2841
EaeI	YGGCCR	1 cut(s)	622
Eco130I	CCWWGG	2 cut(s)	545, 1592
Eco147I	AGGCCT	1 cut(s)	2472
Eco24I	GRGCYC	1 cut(s)	1740
Eco31I	GGTCTC	2 cut(s)	2278, 2721
Eco32I	GATATC	1 cut(s)	796
Eco47I	GGWCC	4 cut(s)	350, 401, 549, 816
Eco57I	CTGAAG	1 cut(s)	2493
Eco72I	CACGTG	1 cut(s)	1101
Eco81I	CCTNAGG	1 cut(s)	693
Eco88I	CYCGRG	1 cut(s)	819
Eco91I	GGTNACC	1 cut(s)	2450
EcoO109I	RGGNCCY	2 cut(s)	549, 816
EcoO65I	GGTNACC	1 cut(s)	2450
EcoRII	CCWGG	4 cut(s)	430, 502, 1303, 1812
EcoRV	GATATC	1 cut(s)	796
EcoT14I	CCWWGG	2 cut(s)	545, 1592
EcoT22I	ATGCAT	2 cut(s)	660, 2222
EcoT38I	GRGCYC	1 cut(s)	1740
EgeI	GGCGCC	2 cut(s)	1777, 3015
EheI	GGCGCC	2 cut(s)	1777, 3015
ErhI	CCWWGG	2 cut(s)	545, 1592
FalI	AAGNNNNNCTT	2 cut(s)	672, 704
FaqI	GGGAC	3 cut(s)	101, 324, 802
FauI	CCCGC	1 cut(s)	2338
FauNDI	CATATG	1 cut(s)	2128
FblI	GTMKAC	3 cut(s)	1157, 2773, 3100
FriOI	GRGCYC	1 cut(s)	1740
FspBI	CTAG	8 cut(s)	519, 773, 1587, 1593, 1859, 2039, 2121, 3060
GlaI	GCGC	7 cut(s)	370, 611, 1682, 1777, 2093, 2708, 3015
GsaI	CCCAGC	2 cut(s)	179, 2898
GsuI	CTGGAG	1 cut(s)	1026
HaeII	RGCGCY	2 cut(s)	1779, 3017
HaeIII	GGCC	5 cut(s)	624, 1170, 2037, 2472, 3033
HapII	CCGG	5 cut(s)	820, 1143, 1437, 2112, 3088
HgaI	GACGC	1 cut(s)	1155
HhaI	GCGC	7 cut(s)	371, 612, 1683, 1778, 2094, 2709, 3016
Hin1I	GRCGYC	2 cut(s)	1776, 3014
Hin6I	GCGC	7 cut(s)	369, 610, 1681, 1776, 2092, 2707, 3014
HinP1I	GCGC	7 cut(s)	369, 610, 1681, 1776, 2092, 2707, 3014
HincII	GTYRAC	1 cut(s)	2597
HindII	GTYRAC	1 cut(s)	2597
HindIII	AAGCTT	3 cut(s)	164, 1257, 2693
HpaII	CCGG	5 cut(s)	820, 1143, 1437, 2112, 3088
HphI	GGTGA	6 cut(s)	22, 803, 907, 1391, 1517, 2444
Hpy188III	TCNNGA	8 cut(s)	47, 158, 535, 1284, 2080, 2305, 2360, 3023
HpyCH4III	ACNGT	9 cut(s)	879, 1089, 1155, 1561, 1623, 1646, 1801, 2801, 2985
HpyCH4IV	ACGT	4 cut(s)	193, 203, 1100, 2247
HpySE526I	ACGT	4 cut(s)	193, 203, 1100, 2247
Hsp92I	GRCGYC	2 cut(s)	1776, 3014
HspAI	GCGC	7 cut(s)	369, 610, 1681, 1776, 2092, 2707, 3014
KasI	GGCGCC	2 cut(s)	1775, 3013
KflI	GGGWCCC	1 cut(s)	816
Kzo9I	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
LmnI	GCTCC	6 cut(s)	18, 502, 1183, 1306, 2538, 2743
MaeI	CTAG	8 cut(s)	519, 773, 1587, 1593, 1859, 2039, 2121, 3060
MaeII	ACGT	4 cut(s)	193, 203, 1100, 2247
MaeIII	GTNAC	6 cut(s)	562, 1096, 1617, 1630, 2450, 2795
MalI	GATC	9 cut(s)	25, 29, 288, 316, 687, 724, 1282, 1956, 2640
MboI	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
MboII	GAAGA	6 cut(s)	188, 680, 761, 1261, 2499, 3118
MfeI	CAATTG	2 cut(s)	223, 2379
MflI	RGATCY	3 cut(s)	314, 722, 1954
MhlI	GDGCHC	5 cut(s)	600, 937, 1199, 1319, 1740
MlsI	TGGCCA	1 cut(s)	624
MluNI	TGGCCA	1 cut(s)	624
Mly113I	GGCGCC	2 cut(s)	1776, 3014
MlyI	GAGTC	4 cut(s)	226, 1344, 1982, 2829
MmeI	TCCRAC	5 cut(s)	326, 757, 1025, 2590, 2788
Mox20I	TGGCCA	1 cut(s)	624
Mph1103I	ATGCAT	2 cut(s)	660, 2222
MroXI	GAANNNNTTC	1 cut(s)	1490
MscI	TGGCCA	1 cut(s)	624
MslI	CAYNNNNRTG	1 cut(s)	1322
Msp20I	TGGCCA	1 cut(s)	624
MspA1I	CMGCKG	6 cut(s)	527, 587, 2201, 2333, 2588, 2886
MspCI	CTTAAG	1 cut(s)	2830
MspI	CCGG	5 cut(s)	820, 1143, 1437, 2112, 3088
MspR9I	CCNGG	8 cut(s)	432, 504, 820, 821, 1305, 1814, 2113, 3088
MunI	CAATTG	2 cut(s)	223, 2379
Mva1269I	GAATGC	4 cut(s)	170, 612, 962, 1064
MvaI	CCWGG	4 cut(s)	432, 504, 1305, 1814
NarI	GGCGCC	2 cut(s)	1776, 3014
NciI	CCSGG	4 cut(s)	820, 821, 2113, 3088
NdeI	CATATG	1 cut(s)	2128
NdeII	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
NmuCI	GTSAC	4 cut(s)	1096, 1617, 2450, 2795
NsiI	ATGCAT	2 cut(s)	660, 2222
NspI	RCATGY	2 cut(s)	2220, 2975
NspV	TTCGAA	1 cut(s)	199
PaeI	GCATGC	1 cut(s)	2220
PagI	TCATGA	1 cut(s)	2304
PceI	AGGCCT	1 cut(s)	2472
PctI	GAATGC	4 cut(s)	170, 612, 962, 1064
PdmI	GAANNNNTTC	1 cut(s)	1490
PflMI	CCANNNNNTGG	3 cut(s)	365, 431, 2159
PfoI	TCCNGGA	3 cut(s)	1303, 2111, 3086
PinAI	ACCGGT	1 cut(s)	1436
Ple19I	CGATCG	1 cut(s)	26
PleI	GAGTC	4 cut(s)	225, 1344, 1982, 2829
PluTI	GGCGCC	2 cut(s)	1779, 3017
PmaCI	CACGTG	1 cut(s)	1101
PmlI	CACGTG	1 cut(s)	1101
PpsI	GAGTC	4 cut(s)	225, 1344, 1982, 2829
Ppu21I	YACGTR	1 cut(s)	1101
PpuMI	RGGWCCY	2 cut(s)	549, 816
PshBI	ATTAAT	2 cut(s)	2018, 2405
Psp1406I	AACGTT	1 cut(s)	203
Psp5II	RGGWCCY	2 cut(s)	549, 816
Psp6I	CCWGG	4 cut(s)	430, 502, 1303, 1812
PspCI	CACGTG	1 cut(s)	1101
PspEI	GGTNACC	1 cut(s)	2450
PspFI	CCCAGC	2 cut(s)	175, 2894
PspGI	CCWGG	4 cut(s)	430, 502, 1303, 1812
PspPI	GGNCC	5 cut(s)	350, 401, 549, 816, 3032
PspPPI	RGGWCCY	2 cut(s)	549, 816
PstNI	CAGNNNCTG	2 cut(s)	804, 2516
PsuI	RGATCY	3 cut(s)	314, 722, 1954
PvuI	CGATCG	1 cut(s)	26
PvuII	CAGCTG	4 cut(s)	527, 587, 2201, 2588
RsaI	GTAC	6 cut(s)	709, 834, 1864, 2024, 2125, 2684
RsaNI	GTAC	6 cut(s)	708, 833, 1863, 2023, 2124, 2683
RseI	CAYNNNNRTG	1 cut(s)	1322
Sau3AI	GATC	9 cut(s)	23, 27, 286, 314, 685, 722, 1280, 1954, 2638
Sau96I	GGNCC	5 cut(s)	350, 401, 549, 816, 3032
SchI	GAGTC	4 cut(s)	226, 1344, 1982, 2829
ScrFI	CCNGG	8 cut(s)	432, 504, 820, 821, 1305, 1814, 2113, 3088
SduI	GDGCHC	5 cut(s)	600, 937, 1199, 1319, 1740
SfcI	CTRYAG	2 cut(s)	555, 1137
SfoI	GGCGCC	2 cut(s)	1777, 3015
SfuI	TTCGAA	1 cut(s)	199
SinI	GGWCC	4 cut(s)	350, 401, 549, 816
SmaI	CCCGGG	1 cut(s)	821
SmiMI	CAYNNNNRTG	1 cut(s)	1322
SmlI	CTYRAG	3 cut(s)	2358, 2830, 3021
SmoI	CTYRAG	3 cut(s)	2358, 2830, 3021
SphI	GCATGC	1 cut(s)	2220
SseBI	AGGCCT	1 cut(s)	2472
SsiI	CCGC	6 cut(s)	1047, 1575, 1952, 2331, 2886, 3063
SspDI	GGCGCC	2 cut(s)	1775, 3013
SspI	AATATT	1 cut(s)	2409
SspMI	CTAG	8 cut(s)	519, 773, 1587, 1593, 1859, 2039, 2121, 3060
StuI	AGGCCT	1 cut(s)	2472
StyD4I	CCNGG	8 cut(s)	430, 502, 818, 819, 1303, 1812, 2111, 3086
StyI	CCWWGG	2 cut(s)	545, 1592
TaaI	ACNGT	9 cut(s)	879, 1089, 1155, 1561, 1623, 1646, 1801, 2801, 2985
TaiI	ACGT	4 cut(s)	196, 206, 1103, 2250
TaqI	TCGA	5 cut(s)	26, 199, 1723, 2845, 3170
TatI	WGTACW	2 cut(s)	2022, 2123
TscAI	CASTG	7 cut(s)	625, 921, 1197, 1626, 1651, 1804, 2973
TseFI	GTSAC	4 cut(s)	1096, 1617, 2450, 2795
Tsp45I	GTSAC	4 cut(s)	1096, 1617, 2450, 2795
TspGWI	ACGGA	5 cut(s)	327, 695, 957, 1441, 1915
TspMI	CCCGGG	1 cut(s)	819
TspRI	CASTG	7 cut(s)	625, 921, 1197, 1626, 1651, 1804, 2973
Van91I	CCANNNNNTGG	3 cut(s)	365, 431, 2159
Vha464I	CTTAAG	1 cut(s)	2830
VneI	GTGCAC	1 cut(s)	1315
VpaK11BI	GGWCC	4 cut(s)	350, 401, 549, 816
VspI	ATTAAT	2 cut(s)	2018, 2405
XapI	RAATTY	2 cut(s)	1511, 1937
XceI	RCATGY	2 cut(s)	2220, 2975
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2902
XmaI	CCCGGG	1 cut(s)	819
XmaJI	CCTAGG	1 cut(s)	1592
XmiI	GTMKAC	3 cut(s)	1157, 2773, 3100
XmnI	GAANNNNTTC	1 cut(s)	1490
XspI	CTAG	8 cut(s)	519, 773, 1587, 1593, 1859, 2039, 2121, 3060
Zsp2I	ATGCAT	2 cut(s)	660, 2222

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.