pycom02g01520

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002821
Physical Location & Seq
Forward (+)
909273 .. 912139
2867 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGGCGACGACACCCTCGTCCGCTCTCCACCAAACATTTCTATCCCGAAACTACTCTCCCCTCCATCGAAATTCTCAATTCCGCCATCCCCGTCTCCGTCTCCGTCTCCCCTCTCGCCATCACTCTCACCCCATTCGCGCCACCTCCACCGTCACTCTCCCACCGGAGCGGCGGTCGAGGGCCGAGGAAATCCAAAGCTCCGGCGTTGACCTCGAGCTGCTTGCCTGCCCAGTATGCTACGAACCACTCATCCGAAAGGGGCCGCCGGGTCTCAATCTGCGAGCAATTTACAGGTCGGGGTTCAAGTGCACGAAATGCGACAAGTCGTATTCGAGTAAAGACGTTTACTTGGACCTTACTGTTACTGCTGGGTTGAAGGAGTACGTTGAGGTACAACCGATCCGGACCGAGTTGTTCCGGAGCCCTCTCGTTTCCTTCTTGTATGAAAGGGGTTGGCGCCAGAATTTTAACCTCAGCGGATTTCCAGGTCCCGATGAAGAGTTTAAGATGGCTCAGGACTACTTTAAATCCGCCGAAGGCGGTGTTTTGGTGGATGTGAGCTGTGGGAGCGGCTTGTTTTCGAGGAATTTTGCGAAATCCGGGACATATTCTGCAGTTGTTGCACTTGATTTTTCGGAGAACATGCTCCGGCAGAGTTATGAATTTTTTAAGAGGGATCCTACTCTTTTAGATACTAATCTTGCTCTTGTGAGGGCGGATGTTGCTAGGCTTCCGTTCCCTTCGGGTTCAGTTGATGCTGTCCATGCCGGCGCAGCATTGCATTGCTGGCCCTCTCCTTCCAATGCTATTGCTGAAATAACTCGCATATTACGAAGTGGTGGAGTCTTTGTTGGAACCACTTTTCTGCGGTATACTCCATCTACTCCCTGGATAACCCGGCAACTCAGAGAGAGGGCTCTTCAGGGCTACAACTATTTAACGGAGGAGGAGATCGAGGACTTGTGTGCATCATGCGGTCTCACCAACTACACGAGCAACGTTCAACAAGCTTTCATCATGTTTTCTGCTCAGAAACCTTAA

Protein Analysis

347

Amino Acids

38.87

Weight (kDa)

8.97

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 175 - 287 3.4e-10 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 177 - 330 7.1e-12 Methyltransferase domain
Methyltransf_31 PF13847 180 - 288 6.8e-12 Methyltransferase domain
Methyltransf_11 PF08241 184 - 284 4.3e-21 Methyltransferase domain
Methyltransf_25 PF13649 184 - 281 3.8e-17 Methyltransferase domain
Methyltransf_12 PF08242 184 - 283 8e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 456
AccBSI CCGCTC 3 cut(s) 23, 169, 570
AccI GTMKAC 1 cut(s) 872
AccII CGCG 1 cut(s) 138
AccIII TCCGGA 2 cut(s) 402, 417
AclI AACGTT 1 cut(s) 999
AclWI GGATC 3 cut(s) 394, 671, 684
AcsI RAATTY 4 cut(s) 70, 463, 586, 662
AcuI CTGAAG 1 cut(s) 905
AcyI GRCGYC 1 cut(s) 457
AfaI GTAC 2 cut(s) 383, 393
AgsI TTSAA 3 cut(s) 304, 376, 1004
AjnI CCWGG 2 cut(s) 484, 887
AloI GAACNNNNNNTCC 2 cut(s) 234, 266
AluBI AGCT 4 cut(s) 198, 217, 561, 1010
AluI AGCT 4 cut(s) 198, 217, 561, 1010
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 5 cut(s) 98, 104, 110, 275, 983
Alw44I GTGCAC 1 cut(s) 307
AlwI GGATC 3 cut(s) 394, 671, 684
Ama87I CYCGRG 1 cut(s) 212
Aor13HI TCCGGA 2 cut(s) 402, 417
AoxI GGCC 3 cut(s) 180, 260, 788
ApaLI GTGCAC 1 cut(s) 307
ApeKI GCWGC 2 cut(s) 217, 773
ApoI RAATTY 4 cut(s) 70, 463, 586, 662
AspLEI GCGC 3 cut(s) 140, 459, 773
AspS9I GGNCC 6 cut(s) 180, 260, 352, 405, 488, 789
AsuC2I CCSGG 3 cut(s) 267, 601, 898
AsuHPI GGTGA 2 cut(s) 119, 973
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 3 cut(s) 352, 405, 488
BaeGI GKGCMC 1 cut(s) 311
BamHI GGATCC 1 cut(s) 676
BanI GGYRCC 1 cut(s) 456
BanII GRGCYC 2 cut(s) 425, 919
BauI CACGAG 1 cut(s) 991
Bbv12I GWGCWC 1 cut(s) 311
BbvCI CCTCAGC 1 cut(s) 473
BbvI GCAGC 2 cut(s) 204, 785
BccI CCATC 5 cut(s) 72, 93, 126, 502, 886
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 2 cut(s) 486, 889
BcnI CCSGG 3 cut(s) 267, 601, 898
BcoDI GTCTC 5 cut(s) 98, 104, 110, 275, 983
BfaI CTAG 1 cut(s) 726
BfmI CTRYAG 1 cut(s) 612
BfoI RGCGCY 1 cut(s) 460
BisI GCNGC 5 cut(s) 170, 218, 263, 571, 774
BlsI GCNGC 5 cut(s) 171, 219, 264, 572, 775
Bme1390I CCNGG 5 cut(s) 267, 486, 601, 889, 898
Bme18I GGWCC 3 cut(s) 352, 405, 488
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 6 cut(s) 180, 260, 352, 405, 488, 789
BmiI GGNNCC 6 cut(s) 261, 422, 458, 490, 678, 856
BmrFI CCNGG 5 cut(s) 267, 486, 601, 889, 898
BmrI ACTGGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 745, 977
BmuI ACTGGG 1 cut(s) 224
Bpu10I CCTNAGC 2 cut(s) 473, 513
BpuMI CCSGG 3 cut(s) 267, 601, 898
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 1 cut(s) 457
BsaI GGTCTC 2 cut(s) 275, 983
BsaJI CCNNGG 2 cut(s) 183, 887
BsaWI WCCGGW 3 cut(s) 163, 402, 417
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 834, 864
Bse118I RCCGGY 1 cut(s) 767
Bse1I ACTGG 1 cut(s) 230
Bse3DI GCAATG 2 cut(s) 776, 781
Bse8I GATNNNNATC 1 cut(s) 696
BseAI TCCGGA 2 cut(s) 402, 417
BseBI CCWGG 2 cut(s) 486, 889
BseDI CCNNGG 2 cut(s) 183, 887
BseGI GGATG 4 cut(s) 85, 249, 559, 724
BseJI GATNNNNATC 1 cut(s) 696
BseMI GCAATG 2 cut(s) 776, 781
BseMII CTCAG 3 cut(s) 487, 527, 919
BseNI ACTGG 1 cut(s) 230
BseRI GAGGAG 2 cut(s) 959, 962
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 2 cut(s) 204, 785
BseYI CCCAGC 1 cut(s) 368
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 3 cut(s) 182, 262, 790
BshNI GGYRCC 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 311
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 9 cut(s) 164, 201, 266, 403, 418, 600, 649, 768, 898
BslFI GGGAC 2 cut(s) 474, 616
BsmAI GTCTC 5 cut(s) 98, 104, 110, 275, 983
BsmBI CGTCTC 3 cut(s) 98, 104, 110
BsmFI GGGAC 2 cut(s) 474, 616
BsnI GGCC 3 cut(s) 182, 262, 790
Bso31I GGTCTC 2 cut(s) 275, 983
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 311, 425, 919
Bsp13I TCCGGA 2 cut(s) 402, 417
Bsp143I GATC 3 cut(s) 399, 676, 951
BspANI GGCC 3 cut(s) 182, 262, 790
BspCNI CTCAG 3 cut(s) 486, 526, 918
BspEI TCCGGA 2 cut(s) 402, 417
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 6 cut(s) 261, 422, 458, 490, 678, 856
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 3 cut(s) 394, 671, 684
BspQI GCTCTTC 1 cut(s) 924
BspT107I GGYRCC 1 cut(s) 456
BspTNI GGTCTC 2 cut(s) 275, 983
BsrBI CCGCTC 3 cut(s) 23, 169, 570
BsrDI GCAATG 2 cut(s) 776, 781
BsrFI RCCGGY 1 cut(s) 767
BsrI ACTGG 1 cut(s) 230
BssAI RCCGGY 1 cut(s) 767
BssECI CCNNGG 2 cut(s) 183, 887
BssMI GATC 3 cut(s) 399, 676, 951
BssNAI GTATAC 1 cut(s) 873
BssNI GRCGYC 1 cut(s) 457
BssSI CACGAG 1 cut(s) 991
Bst1107I GTATAC 1 cut(s) 873
Bst2BI CACGAG 1 cut(s) 991
Bst2UI CCWGG 2 cut(s) 486, 889
Bst4CI ACNGT 2 cut(s) 151, 361
Bst6I CTCTTC 2 cut(s) 492, 924
BstACI GRCGYC 1 cut(s) 457
BstAPI GCANNNNNTGC 2 cut(s) 315, 620
BstC8I GCNNGC 5 cut(s) 222, 226, 282, 769, 788
BstDEI CTNAG 4 cut(s) 473, 513, 905, 1029
BstF5I GGATG 4 cut(s) 85, 249, 559, 724
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 3 cut(s) 140, 459, 773
BstKTI GATC 3 cut(s) 402, 679, 954
BstMAI GTCTC 5 cut(s) 98, 104, 110, 275, 983
BstMBI GATC 3 cut(s) 399, 676, 951
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 8 cut(s) 234, 315, 567, 620, 722, 764, 773, 787
BstNI CCWGG 2 cut(s) 486, 889
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 5 cut(s) 265, 484, 599, 887, 896
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 311
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 204, 785
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BstZ17I GTATAC 1 cut(s) 873
BsuRI GGCC 3 cut(s) 182, 262, 790
BtsCI GGATG 4 cut(s) 85, 249, 559, 724
Cac8I GCNNGC 5 cut(s) 222, 226, 282, 769, 788
CfoI GCGC 3 cut(s) 140, 459, 773
Cfr10I RCCGGY 1 cut(s) 767
Cfr13I GGNCC 6 cut(s) 180, 260, 352, 405, 488, 789
CpoI CGGWCCG 1 cut(s) 405
Csp6I GTAC 2 cut(s) 382, 392
CspI CGGWCCG 1 cut(s) 405
CviAII CATG 4 cut(s) 643, 764, 972, 1018
CviQI GTAC 2 cut(s) 382, 392
DdeI CTNAG 4 cut(s) 473, 513, 905, 1029
DinI GGCGCC 1 cut(s) 458
DpnI GATC 3 cut(s) 401, 678, 953
DpnII GATC 3 cut(s) 399, 676, 951
DraI TTTAAA 1 cut(s) 526
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 492, 924
EarI CTCTTC 2 cut(s) 492, 924
EciI GGCGGA 3 cut(s) 71, 520, 731
Eco24I GRGCYC 2 cut(s) 425, 919
Eco31I GGTCTC 2 cut(s) 275, 983
Eco47I GGWCC 3 cut(s) 352, 405, 488
Eco57I CTGAAG 1 cut(s) 905
Eco88I CYCGRG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 2 cut(s) 484, 887
EcoT38I GRGCYC 2 cut(s) 425, 919
EgeI GGCGCC 1 cut(s) 458
EheI GGCGCC 1 cut(s) 458
Esp3I CGTCTC 3 cut(s) 98, 104, 110
FaeI CATG 4 cut(s) 646, 767, 975, 1021
FaqI GGGAC 2 cut(s) 474, 616
FatI CATG 4 cut(s) 642, 763, 971, 1017
FblI GTMKAC 1 cut(s) 872
Fnu4HI GCNGC 5 cut(s) 170, 218, 263, 571, 774
FokI GGATG 4 cut(s) 72, 236, 566, 731
FriOI GRGCYC 2 cut(s) 425, 919
Fsp4HI GCNGC 5 cut(s) 170, 218, 263, 571, 774
FspBI CTAG 1 cut(s) 726
GlaI GCGC 3 cut(s) 139, 458, 772
GluI GCNGC 5 cut(s) 170, 218, 263, 571, 774
GsaI CCCAGC 1 cut(s) 372
HaeII RGCGCY 1 cut(s) 460
HaeIII GGCC 3 cut(s) 182, 262, 790
HapII CCGG 9 cut(s) 164, 201, 266, 403, 418, 600, 649, 768, 898
HhaI GCGC 3 cut(s) 140, 459, 773
Hin1I GRCGYC 1 cut(s) 457
Hin1II CATG 4 cut(s) 646, 767, 975, 1021
Hin6I GCGC 3 cut(s) 138, 457, 771
HinP1I GCGC 3 cut(s) 138, 457, 771
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 1008
HinfI GANTC 1 cut(s) 843
HpaII CCGG 9 cut(s) 164, 201, 266, 403, 418, 600, 649, 768, 898
HphI GGTGA 2 cut(s) 119, 973
Hpy166II GTNNAC 4 cut(s) 208, 309, 346, 873
Hpy188I TCNGA 4 cut(s) 254, 637, 908, 1032
Hpy188III TCNNGA 5 cut(s) 45, 403, 418, 491, 515
Hpy8I GTNNAC 4 cut(s) 208, 309, 346, 873
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 5 cut(s) 370, 445, 530, 750, 807
HpyCH4III ACNGT 2 cut(s) 151, 361
HpyCH4IV ACGT 3 cut(s) 342, 384, 999
HpyCH4V TGCA 5 cut(s) 309, 614, 623, 781, 968
HpyF10VI GCNNNNNNNGC 8 cut(s) 234, 315, 567, 620, 722, 764, 773, 787
HpyF3I CTNAG 4 cut(s) 473, 513, 905, 1029
HpySE526I ACGT 3 cut(s) 342, 384, 999
Hsp92I GRCGYC 1 cut(s) 457
Hsp92II CATG 4 cut(s) 646, 767, 975, 1021
HspAI GCGC 3 cut(s) 138, 457, 771
KasI GGCGCC 1 cut(s) 456
Kpn2I TCCGGA 2 cut(s) 402, 417
KroI GCCGGC 1 cut(s) 767
KroNI GCCGGC 1 cut(s) 769
Kzo9I GATC 3 cut(s) 399, 676, 951
LguI GCTCTTC 1 cut(s) 924
LmnI GCTCC 5 cut(s) 166, 203, 420, 567, 651
Lsp1109I GCAGC 2 cut(s) 204, 785
LweI GCATC 2 cut(s) 745, 977
MaeI CTAG 1 cut(s) 726
MaeII ACGT 3 cut(s) 342, 384, 999
MaeIII GTNAC 2 cut(s) 151, 361
MalI GATC 3 cut(s) 401, 678, 953
MbiI CCGCTC 3 cut(s) 23, 169, 570
MboI GATC 3 cut(s) 399, 676, 951
MboII GAAGA 2 cut(s) 509, 911
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 3 cut(s) 311, 425, 919
MluCI AATT 6 cut(s) 70, 77, 285, 463, 586, 662
Mly113I GGCGCC 1 cut(s) 457
MlyI GAGTC 1 cut(s) 852
MmeI TCCRAC 1 cut(s) 832
MroI TCCGGA 2 cut(s) 402, 417
MroNI GCCGGC 1 cut(s) 767
MseI TTAA 6 cut(s) 468, 504, 525, 669, 938, 1039
MspA1I CMGCKG 1 cut(s) 477
MspI CCGG 9 cut(s) 164, 201, 266, 403, 418, 600, 649, 768, 898
MspR9I CCNGG 5 cut(s) 267, 486, 601, 889, 898
MvaI CCWGG 2 cut(s) 486, 889
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 8 cut(s) 234, 315, 567, 620, 722, 764, 773, 787
NaeI GCCGGC 1 cut(s) 769
NarI GGCGCC 1 cut(s) 457
NciI CCSGG 3 cut(s) 267, 601, 898
NdeII GATC 3 cut(s) 399, 676, 951
NgoMIV GCCGGC 1 cut(s) 767
NlaIII CATG 4 cut(s) 646, 767, 975, 1021
NlaIV GGNNCC 6 cut(s) 261, 422, 458, 490, 678, 856
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 151
NspI RCATGY 1 cut(s) 646
PaeR7I CTCGAG 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 924
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 829
PdiI GCCGGC 1 cut(s) 769
PfoI TCCNGGA 1 cut(s) 599
PkrI GCNGC 5 cut(s) 171, 219, 264, 572, 775
PleI GAGTC 1 cut(s) 851
PluTI GGCGCC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 851
PpuMI RGGWCCY 1 cut(s) 488
Psp1406I AACGTT 1 cut(s) 999
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 2 cut(s) 484, 887
PspFI CCCAGC 1 cut(s) 368
PspGI CCWGG 2 cut(s) 484, 887
PspN4I GGNNCC 6 cut(s) 261, 422, 458, 490, 678, 856
PspPI GGNCC 6 cut(s) 180, 260, 352, 405, 488, 789
PspPPI RGGWCCY 1 cut(s) 488
PspXI VCTCGAGB 1 cut(s) 212
PstI CTGCAG 1 cut(s) 616
PsuI RGATCY 1 cut(s) 676
RsaI GTAC 2 cut(s) 383, 393
RsaNI GTAC 2 cut(s) 382, 392
Rsr2I CGGWCCG 1 cut(s) 405
RsrII CGGWCCG 1 cut(s) 405
SapI GCTCTTC 1 cut(s) 924
SaqAI TTAA 6 cut(s) 468, 504, 525, 669, 938, 1039
SatI GCNGC 5 cut(s) 170, 218, 263, 571, 774
Sau3AI GATC 3 cut(s) 399, 676, 951
Sau96I GGNCC 6 cut(s) 180, 260, 352, 405, 488, 789
SchI GAGTC 1 cut(s) 852
ScrFI CCNGG 5 cut(s) 267, 486, 601, 889, 898
SduI GDGCHC 3 cut(s) 311, 425, 919
SfaNI GCATC 2 cut(s) 745, 977
SfcI CTRYAG 1 cut(s) 612
SfoI GGCGCC 1 cut(s) 458
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 3 cut(s) 352, 405, 488
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 6 cut(s) 70, 77, 285, 463, 586, 662
SspDI GGCGCC 1 cut(s) 456
SspMI CTAG 1 cut(s) 726
StyD4I CCNGG 5 cut(s) 265, 484, 599, 887, 896
TaaI ACNGT 2 cut(s) 151, 361
TaiI ACGT 3 cut(s) 345, 387, 1002
TaqI TCGA 6 cut(s) 67, 176, 213, 332, 581, 954
TaqII GACCGA 1 cut(s) 422
TasI AATT 6 cut(s) 70, 77, 285, 463, 586, 662
TauI GCSGC 3 cut(s) 172, 265, 573
Tru1I TTAA 6 cut(s) 468, 504, 525, 669, 938, 1039
Tru9I TTAA 6 cut(s) 468, 504, 525, 669, 938, 1039
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 2 cut(s) 217, 773
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 4 cut(s) 459, 510, 675, 1003
TspGWI ACGGA 4 cut(s) 86, 92, 723, 956
VneI GTGCAC 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 352, 405, 488
XapI RAATTY 4 cut(s) 70, 463, 586, 662
XceI RCATGY 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 885
XhoI CTCGAG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 872
XspI CTAG 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.