pycom03g01180

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003999
Physical Location & Seq
Reverse (-)
933855 .. 935265
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 783 bp
ATGGTAACGGTAACGGTGCATGCATGCAGGAGCGAGAAAGATGCACCTCCCAATCAATCGACAGACGGATACGACGTTGTAGATGATATCAAAACTGCGGTCGAGAACGTTTGCCCTGGTGTTGTCTCCTGTTCTGACATTTTAGCAATTGCTTCTCAAATTCTTGTTTCTGCGAATGGAGGGCCAACGTGGGAAGCTCAATTGGGAAGAAGAGACAGTAGGACAGCCAACCAAGCTGGTGCCAGTGCCATTCCAAGCTTTCTCGAAAACCTTGAGCAAATTTCACAAAAATTTCGCGATGCAGGATTAGATTCCACTGATCTGGTTGCTTTATCAGGTAACCCTAGTAATAGACACTTGTTTCCTTTCTTAGGTGCTCATACATTTGGACGGGCAAGATGTTCAACTTTCGTTCACCGCCTCTACAATTTCAGCGGCACTGGCAATCCGGACCCGACCATTGAAGCCGGATACTTGGAAACCCTCCGCCAAGCGTGTCCTCAAAACGGCAGCGGAGATACGTTGAATGACCTTGACCAGTCCACCCGCGATGCCTTCGACAACCACTACTTTACAAATCTCCAAAACAACCGCGGGCTTCTCCAGACCGATCAGGTGCTGTTCTCAACTAGCGGAGACACCGTTGCCATTGTAGACCGCTTTGCAAATAGCCAAAGTGACTTCTTTGATAGCTTTGGTCAGTCCATGATCAATATGGGGAATATAGGAGTTTTGACAGGCACCAATGGAGAGATTAGGACTGATTGCAGAAGAGTTAATTAA

Protein Analysis

261

Amino Acids

28.11

Weight (kDa)

4.76

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 10 - 224 7.2e-62 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 239, 740
AccI GTMKAC 1 cut(s) 654
AccII CGCG 3 cut(s) 297, 549, 594
AccIII TCCGGA 1 cut(s) 448
AclI AACGTT 1 cut(s) 108
AcsI RAATTY 3 cut(s) 159, 279, 290
AfiI CCNNNNNNNGG 2 cut(s) 371, 506
AgsI TTSAA 3 cut(s) 405, 464, 526
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 4 cut(s) 197, 236, 258, 693
AluI AGCT 4 cut(s) 197, 236, 258, 693
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 3 cut(s) 130, 207, 630
AlwNI CAGNNNCTG 1 cut(s) 619
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 510
ApoI RAATTY 3 cut(s) 159, 279, 290
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AspS9I GGNCC 2 cut(s) 182, 451
AsuHPI GGTGA 1 cut(s) 407
AvaII GGWCC 1 cut(s) 451
BanI GGYRCC 2 cut(s) 239, 740
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 522
BceAI ACGGC 1 cut(s) 523
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciT130I CCWGG 1 cut(s) 117
BciVI GTATCC 2 cut(s) 62, 464
BclI TGATCA 1 cut(s) 708
BcoDI GTCTC 3 cut(s) 130, 207, 630
BfaI CTAG 2 cut(s) 345, 630
BfuI GTATCC 2 cut(s) 62, 464
BisI GCNGC 2 cut(s) 436, 511
BlsI GCNGC 2 cut(s) 437, 512
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 2 cut(s) 182, 451
BmiI GGNNCC 3 cut(s) 241, 453, 742
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 3 cut(s) 31, 289, 541
BpmI CTGGAG 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 115, 592
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 2 cut(s) 627, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 506
Bse1I ACTGG 3 cut(s) 243, 445, 538
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 115, 592
BseLI CCNNNNNNNGG 2 cut(s) 371, 506
BseNI ACTGG 3 cut(s) 243, 445, 538
BseXI GCAGC 1 cut(s) 522
Bsh1236I CGCG 3 cut(s) 297, 549, 594
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 1 cut(s) 184
BshNI GGYRCC 2 cut(s) 239, 740
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 2 cut(s) 449, 468
BslI CCNNNNNNNGG 2 cut(s) 371, 506
BsmAI GTCTC 3 cut(s) 130, 207, 630
BsnI GGCC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 379
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 3 cut(s) 319, 610, 708
Bsp68I TCGCGA 1 cut(s) 297
BspANI GGCC 1 cut(s) 184
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 3 cut(s) 297, 549, 594
BspLI GGNNCC 3 cut(s) 241, 453, 742
BspT107I GGYRCC 2 cut(s) 239, 740
BsrI ACTGG 3 cut(s) 243, 445, 538
BssECI CCNNGG 2 cut(s) 115, 592
BssMI GATC 3 cut(s) 319, 610, 708
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 4 cut(s) 10, 16, 218, 643
Bst6I CTCTTC 2 cut(s) 205, 766
BstC8I GCNNGC 3 cut(s) 21, 25, 596
BstDEI CTNAG 1 cut(s) 370
BstDSI CCRYGG 1 cut(s) 592
BstEII GGTNACC 1 cut(s) 338
BstENI CCTNNNNNAGG 1 cut(s) 369
BstFNI CGCG 3 cut(s) 297, 549, 594
BstKTI GATC 3 cut(s) 322, 613, 711
BstMAI GTCTC 3 cut(s) 130, 207, 630
BstMBI GATC 3 cut(s) 319, 610, 708
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 233, 441
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 2 cut(s) 23, 27
BstPI GGTNACC 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 115
BstUI CGCG 3 cut(s) 297, 549, 594
BstV1I GCAGC 1 cut(s) 522
BstXI CCANNNNNNTGG 1 cut(s) 322
BsuI GTATCC 2 cut(s) 62, 464
BsuRI GGCC 1 cut(s) 184
BtgI CCRYGG 1 cut(s) 592
BtgZI GCGATG 2 cut(s) 312, 564
BtsIMutI CAGTG 3 cut(s) 250, 315, 438
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 21, 25, 596
CaiI CAGNNNCTG 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 182, 451
Cfr42I CCGCGG 1 cut(s) 595
CviAII CATG 3 cut(s) 20, 24, 706
CviJI RGCY 9 cut(s) 184, 197, 227, 236, 258, 467, 598, 672, 693
CviKI_1 RGCY 9 cut(s) 184, 197, 227, 236, 258, 467, 598, 672, 693
DdeI CTNAG 1 cut(s) 370
DpnI GATC 3 cut(s) 321, 612, 710
DpnII GATC 3 cut(s) 319, 610, 708
Eam1104I CTCTTC 2 cut(s) 205, 766
EarI CTCTTC 2 cut(s) 205, 766
EciI GGCGGA 1 cut(s) 476
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 451
Eco91I GGTNACC 1 cut(s) 338
EcoNI CCTNNNNNAGG 1 cut(s) 369
EcoO65I GGTNACC 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 115
EcoRV GATATC 1 cut(s) 88
EcoT22I ATGCAT 1 cut(s) 25
FaeI CATG 3 cut(s) 23, 27, 709
FaiI YATR 6 cut(s) 21, 25, 381, 707, 716, 725
FatI CATG 3 cut(s) 19, 23, 705
FauI CCCGC 2 cut(s) 554, 587
FbaI TGATCA 1 cut(s) 708
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 2 cut(s) 436, 511
Fsp4HI GCNGC 2 cut(s) 436, 511
FspBI CTAG 2 cut(s) 345, 630
GluI GCNGC 2 cut(s) 436, 511
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 1 cut(s) 184
HapII CCGG 2 cut(s) 449, 468
Hin1II CATG 3 cut(s) 23, 27, 709
HindIII AAGCTT 1 cut(s) 256
HinfI GANTC 1 cut(s) 311
HpaII CCGG 2 cut(s) 449, 468
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 3 cut(s) 415, 543, 655
Hpy188I TCNGA 1 cut(s) 136
Hpy188III TCNNGA 5 cut(s) 103, 263, 296, 449, 604
Hpy8I GTNNAC 3 cut(s) 415, 543, 655
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 565
HpyCH4III ACNGT 4 cut(s) 10, 16, 218, 643
HpyCH4IV ACGT 4 cut(s) 75, 108, 188, 521
HpyCH4V TGCA 7 cut(s) 19, 23, 27, 44, 302, 665, 768
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 441
HpyF3I CTNAG 1 cut(s) 370
HpySE526I ACGT 4 cut(s) 75, 108, 188, 521
Hsp92II CATG 3 cut(s) 23, 27, 709
Kpn2I TCCGGA 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 708
KspI CCGCGG 1 cut(s) 595
Kzo9I GATC 3 cut(s) 319, 610, 708
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 1 cut(s) 522
LweI GCATC 3 cut(s) 31, 289, 541
MaeI CTAG 2 cut(s) 345, 630
MaeII ACGT 4 cut(s) 75, 108, 188, 521
MaeIII GTNAC 4 cut(s) 4, 10, 338, 677
MalI GATC 3 cut(s) 321, 612, 710
MboI GATC 3 cut(s) 319, 610, 708
MboII GAAGA 3 cut(s) 219, 222, 783
MfeI CAATTG 2 cut(s) 147, 200
MhlI GDGCHC 1 cut(s) 379
MluCI AATT 7 cut(s) 147, 159, 200, 279, 290, 427, 778
MnlI CCTC 5 cut(s) 57, 173, 431, 494, 510
Mph1103I ATGCAT 1 cut(s) 25
MroI TCCGGA 1 cut(s) 448
MseI TTAA 2 cut(s) 777, 781
MspA1I CMGCKG 3 cut(s) 435, 513, 594
MspI CCGG 2 cut(s) 449, 468
MspR9I CCNGG 1 cut(s) 117
MunI CAATTG 2 cut(s) 147, 200
MvaI CCWGG 1 cut(s) 117
MvnI CGCG 3 cut(s) 297, 549, 594
MwoI GCNNNNNNNGC 2 cut(s) 233, 441
NdeII GATC 3 cut(s) 319, 610, 708
NlaIII CATG 3 cut(s) 23, 27, 709
NlaIV GGNNCC 3 cut(s) 241, 453, 742
NmuCI GTSAC 1 cut(s) 677
NruI TCGCGA 1 cut(s) 297
NsiI ATGCAT 1 cut(s) 25
NspI RCATGY 2 cut(s) 23, 27
PacI TTAATTAA 1 cut(s) 781
PaeI GCATGC 2 cut(s) 23, 27
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 1 cut(s) 311
PkrI GCNGC 2 cut(s) 437, 512
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 115
PspEI GGTNACC 1 cut(s) 338
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 241, 453, 742
PspPI GGNCC 2 cut(s) 182, 451
PstNI CAGNNNCTG 1 cut(s) 619
RruI TCGCGA 1 cut(s) 297
SacII CCGCGG 1 cut(s) 595
SaqAI TTAA 2 cut(s) 777, 781
SatI GCNGC 2 cut(s) 436, 511
Sau3AI GATC 3 cut(s) 319, 610, 708
Sau96I GGNCC 2 cut(s) 182, 451
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 379
SfaNI GCATC 3 cut(s) 31, 289, 541
Sfr303I CCGCGG 1 cut(s) 595
SgrBI CCGCGG 1 cut(s) 595
SinI GGWCC 1 cut(s) 451
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SphI GCATGC 2 cut(s) 23, 27
Sse9I AATT 7 cut(s) 147, 159, 200, 279, 290, 427, 778
SspMI CTAG 2 cut(s) 345, 630
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 4 cut(s) 10, 16, 218, 643
TaiI ACGT 4 cut(s) 78, 111, 191, 524
TaqI TCGA 4 cut(s) 59, 102, 264, 558
TaqII GACCGA 1 cut(s) 623
TasI AATT 7 cut(s) 147, 159, 200, 279, 290, 427, 778
TauI GCSGC 1 cut(s) 438
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 2 cut(s) 777, 781
Tru9I TTAA 2 cut(s) 777, 781
TscAI CASTG 3 cut(s) 250, 322, 445
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 1 cut(s) 510
Tsp45I GTSAC 1 cut(s) 677
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 3 cut(s) 250, 322, 445
VpaK11BI GGWCC 1 cut(s) 451
XagI CCTNNNNNAGG 1 cut(s) 369
XapI RAATTY 3 cut(s) 159, 279, 290
XceI RCATGY 2 cut(s) 23, 27
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 2 cut(s) 345, 630
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.