pycom03g10350

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002391
Physical Location & Seq
Forward (+)
9495851 .. 9500331
4481 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1095 bp
ATGTCGCAGAACGGGAAATTGACGCCGAACCTGGACCAGCAGAGCACGAAGCTTCTGAATCTGACGGTTCTGCAGCGAATCGACCCCTCCGTCGAGGAAATTCTCATTACCTCCACTCACGTCACCTTCTACGAGTTCAACACTGATCTCAGCGAATGGAGTCGCAAGGACGTCGAAGGATCGCTCTTCGTGGTCAAGAGGAATGTGCAACCTCGGTTTCAGTTCATTGTGATGAACCGGCGGAATAGTGCAGAAAATTTGGTGGAGGATCTCTTGGGAGATTTTGAATTCGAAGTCCAACATCCATATCTATTGTATCGAAATGCGGCTCGAGAGGTCAATGGTATTTGGTTCTACAATCCAAGTGAATGTGAGGAGGTGGCAAATATGTTTAACAGGATTCTTAGTGCATATTCCAAGGTACCTCAAAACTCAATGGTATCACCAACAAAGAGTGAGTTTGAGGAACTGGAAGCAGTCCCAAGCATGGTTATTATGGATGGTCCTTTGGAGCCATCGTCATCAACTGCCTCTCATGTCACAGATGTTCCGGATGATCCGGCTTTTGTGAACTTCTTCAGTACGGCTATGACTGTCGTGAATTCTTCAACTACAGCAATTACTGGACAGCCTTACCAATCATCTTCGACAGCCTATCTACCTTCTCATCCATTCGGTGTTGCACCTTCAAGTATTCCTGGCATGCGAATTCCATGTGCTCCACTGACAACTTTATCCCCTTTCGTTAACCTCCCTGAATCCCACAACACCACCAACTTGGCCAGTAATCTTGTAAAGCCATCTTTTTTTGTTCATCCTTCCTCTTCAGCAACAACGATGCCAGCTGTCACTGCATCAAGCCGTGCTGCTTCTCTTCTTCACCCTGCTGTAAGTGTGCAACGTCCATATGGTGCTCCTTTGTTACAGCCTTTTCCACCGCCAGCCCCTCCACCATCTCTCACTGCTGCTTCTTTCCCTCCTAACTATGGGCCACTTAATAGAGATGAAGTACGAAATGCACTCATGCTGCTTGTACAGGATAATCAATTCATCGACATGGTATATGGAGCACTCCAGAAGGCACACCACTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 map04622
Pfam Domains
Protein Families
OTU

Protein Analysis

365

Amino Acids

40.08

Weight (kDa)

5.26

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCP1 PF06058 17 - 132 4.3e-44 Dcp1-like decapping family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AatII GACGTC 1 cut(s) 174
Acc65I GGTACC 1 cut(s) 421
AccB1I GGYRCC 1 cut(s) 421
AccIII TCCGGA 1 cut(s) 550
AciI CCGC 3 cut(s) 241, 326, 938
AclWI GGATC 3 cut(s) 187, 276, 551
AcoI YGGCCR 1 cut(s) 780
AcsI RAATTY 5 cut(s) 99, 256, 287, 601, 708
AcuI CTGAAG 2 cut(s) 562, 810
AcyI GRCGYC 2 cut(s) 23, 171
AfaI GTAC 4 cut(s) 423, 583, 1011, 1035
AfiI CCNNNNNNNGG 2 cut(s) 487, 986
AgsI TTSAA 4 cut(s) 139, 287, 609, 690
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 2 cut(s) 30, 697
AluBI AGCT 2 cut(s) 52, 845
AluI AGCT 2 cut(s) 52, 845
Alw21I GWGCWC 4 cut(s) 47, 721, 916, 1072
AlwI GGATC 3 cut(s) 187, 276, 551
Ama87I CYCGRG 1 cut(s) 330
Aor13HI TCCGGA 1 cut(s) 550
AoxI GGCC 2 cut(s) 780, 989
ApeKI GCWGC 4 cut(s) 73, 866, 965, 1027
ApoI RAATTY 5 cut(s) 99, 256, 287, 601, 708
Asp700I GAANNNNTTC 1 cut(s) 575
Asp718I GGTACC 1 cut(s) 421
AspS9I GGNCC 3 cut(s) 34, 503, 989
AsuHPI GGTGA 3 cut(s) 115, 435, 872
AsuII TTCGAA 1 cut(s) 291
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 34, 503
BalI TGGCCA 1 cut(s) 782
BanI GGYRCC 1 cut(s) 421
Bbv12I GWGCWC 4 cut(s) 47, 721, 916, 1072
BbvI GCAGC 4 cut(s) 85, 853, 952, 1014
BccI CCATC 4 cut(s) 494, 523, 808, 961
BceAI ACGGC 2 cut(s) 600, 846
BcgI CGANNNNNNTGC 3 cut(s) 154, 188, 1071
BciT130I CCWGG 2 cut(s) 32, 699
BfmI CTRYAG 2 cut(s) 71, 612
BisI GCNGC 5 cut(s) 74, 327, 867, 966, 1028
BlsI GCNGC 5 cut(s) 75, 328, 868, 967, 1029
Bme1390I CCNGG 2 cut(s) 32, 699
Bme18I GGWCC 2 cut(s) 34, 503
BmeT110I CYCGRG 1 cut(s) 330
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 34, 503, 989
BmiI GGNNCC 2 cut(s) 423, 513
BmrFI CCNGG 2 cut(s) 32, 699
BmsI GCATC 2 cut(s) 828, 863
BpmI CTGGAG 1 cut(s) 1058
Bpu14I TTCGAA 1 cut(s) 291
BsaHI GRCGYC 2 cut(s) 23, 171
BsaJI CCNNGG 2 cut(s) 212, 417
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 986
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 3 cut(s) 474, 628, 783
BseAI TCCGGA 1 cut(s) 550
BseBI CCWGG 2 cut(s) 32, 699
BseDI CCNNGG 2 cut(s) 212, 417
BseGI GGATG 5 cut(s) 301, 505, 559, 667, 814
BseLI CCNNNNNNNGG 2 cut(s) 487, 986
BseMII CTCAG 1 cut(s) 163
BseNI ACTGG 3 cut(s) 474, 628, 783
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 4 cut(s) 85, 853, 952, 1014
BsgI GTGCAG 1 cut(s) 270
BshFI GGCC 2 cut(s) 782, 991
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 4 cut(s) 47, 721, 916, 1072
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 238, 551, 560
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 2 cut(s) 487, 986
BsmFI GGGAC 1 cut(s) 464
BsnI GGCC 2 cut(s) 782, 991
BsoBI CYCGRG 1 cut(s) 330
Bsp119I TTCGAA 1 cut(s) 291
Bsp1286I GDGCHC 4 cut(s) 47, 721, 916, 1072
Bsp13I TCCGGA 1 cut(s) 550
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 4 cut(s) 145, 179, 268, 556
BspACI CCGC 3 cut(s) 241, 326, 938
BspANI GGCC 2 cut(s) 782, 991
BspCNI CTCAG 1 cut(s) 162
BspEI TCCGGA 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 423, 513
BspMAI CTGCAG 1 cut(s) 75
BspPI GGATC 3 cut(s) 187, 276, 551
BspQI GCTCTTC 1 cut(s) 191
BspT104I TTCGAA 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 421
BsrFI RCCGGY 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 1033
BsrI ACTGG 3 cut(s) 474, 628, 783
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 212, 417
BssMI GATC 4 cut(s) 145, 179, 268, 556
BssNI GRCGYC 2 cut(s) 23, 171
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 2 cut(s) 32, 699
Bst4CI ACNGT 2 cut(s) 67, 595
Bst6I CTCTTC 3 cut(s) 191, 829, 879
BstACI GRCGYC 2 cut(s) 23, 171
BstAUI TGTACA 1 cut(s) 1033
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 3 cut(s) 704, 843, 942
BstDEI CTNAG 2 cut(s) 149, 404
BstF5I GGATG 5 cut(s) 301, 505, 559, 667, 814
BstKTI GATC 4 cut(s) 148, 182, 271, 559
BstMBI GATC 4 cut(s) 145, 179, 268, 556
BstMWI GCNNNNNNNGC 1 cut(s) 851
BstNI CCWGG 2 cut(s) 32, 699
BstNSI RCATGY 1 cut(s) 706
BstSCI CCNGG 2 cut(s) 30, 697
BstSFI CTRYAG 2 cut(s) 71, 612
BstV1I GCAGC 4 cut(s) 85, 853, 952, 1014
BstX2I RGATCY 1 cut(s) 268
BstXI CCANNNNNNTGG 1 cut(s) 778
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 2 cut(s) 782, 991
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 5 cut(s) 301, 505, 559, 667, 814
BtsI GCAGTG 2 cut(s) 849, 960
BtsIMutI CAGTG 4 cut(s) 141, 722, 849, 960
Cac8I GCNNGC 3 cut(s) 704, 843, 942
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 3 cut(s) 34, 503, 989
CseI GACGC 1 cut(s) 31
Csp6I GTAC 4 cut(s) 422, 582, 1010, 1034
CviAII CATG 6 cut(s) 487, 536, 703, 714, 1024, 1057
CviQI GTAC 4 cut(s) 422, 582, 1010, 1034
DdeI CTNAG 2 cut(s) 149, 404
DpnI GATC 4 cut(s) 147, 181, 270, 558
DpnII GATC 4 cut(s) 145, 179, 268, 556
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 780
Eam1104I CTCTTC 3 cut(s) 191, 829, 879
EarI CTCTTC 3 cut(s) 191, 829, 879
EciI GGCGGA 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 417
Eco47I GGWCC 2 cut(s) 34, 503
Eco57I CTGAAG 2 cut(s) 562, 810
Eco88I CYCGRG 1 cut(s) 330
EcoRI GAATTC 3 cut(s) 287, 601, 708
EcoRII CCWGG 2 cut(s) 30, 697
EcoT14I CCWWGG 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 6 cut(s) 490, 539, 706, 717, 1027, 1060
FaqI GGGAC 1 cut(s) 464
FatI CATG 6 cut(s) 486, 535, 702, 713, 1023, 1056
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 5 cut(s) 74, 327, 867, 966, 1028
FokI GGATG 5 cut(s) 288, 512, 566, 654, 801
Fsp4HI GCNGC 5 cut(s) 74, 327, 867, 966, 1028
GluI GCNGC 5 cut(s) 74, 327, 867, 966, 1028
GsuI CTGGAG 1 cut(s) 1058
HaeIII GGCC 2 cut(s) 782, 991
HapII CCGG 3 cut(s) 238, 551, 560
HgaI GACGC 1 cut(s) 31
Hin1I GRCGYC 2 cut(s) 23, 171
Hin1II CATG 6 cut(s) 490, 539, 706, 717, 1027, 1060
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 5 cut(s) 58, 78, 160, 400, 758
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 3 cut(s) 238, 551, 560
HphI GGTGA 3 cut(s) 115, 435, 872
Hpy166II GTNNAC 2 cut(s) 571, 748
Hpy188I TCNGA 2 cut(s) 57, 63
Hpy188III TCNNGA 5 cut(s) 196, 332, 551, 598, 1075
Hpy8I GTNNAC 2 cut(s) 571, 748
Hpy99I CGWCG 2 cut(s) 95, 176
HpyAV CCTTC 6 cut(s) 136, 170, 672, 696, 828, 1072
HpyCH4III ACNGT 2 cut(s) 67, 595
HpyCH4IV ACGT 3 cut(s) 120, 171, 901
HpyCH4V TGCA 8 cut(s) 73, 208, 251, 410, 683, 854, 898, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 851
HpyF3I CTNAG 2 cut(s) 149, 404
HpySE526I ACGT 3 cut(s) 120, 171, 901
Hsp92I GRCGYC 2 cut(s) 23, 171
Hsp92II CATG 6 cut(s) 490, 539, 706, 717, 1027, 1060
Kpn2I TCCGGA 1 cut(s) 550
KpnI GGTACC 1 cut(s) 425
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 4 cut(s) 145, 179, 268, 556
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 4 cut(s) 511, 724, 919, 1067
Lsp1109I GCAGC 4 cut(s) 85, 853, 952, 1014
LweI GCATC 2 cut(s) 828, 863
MaeII ACGT 3 cut(s) 120, 171, 901
MaeIII GTNAC 4 cut(s) 121, 538, 847, 921
MalI GATC 4 cut(s) 147, 181, 270, 558
MboI GATC 4 cut(s) 145, 179, 268, 556
MboII GAAGA 7 cut(s) 178, 568, 597, 636, 816, 866, 869
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 4 cut(s) 47, 721, 916, 1072
MlsI TGGCCA 1 cut(s) 782
MluCI AATT 8 cut(s) 17, 99, 256, 287, 601, 618, 708, 1046
MluNI TGGCCA 1 cut(s) 782
MlyI GAGTC 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 322
Mox20I TGGCCA 1 cut(s) 782
MroI TCCGGA 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 575
MscI TGGCCA 1 cut(s) 782
MseI TTAA 3 cut(s) 393, 747, 996
MslI CAYNNNNRTG 2 cut(s) 230, 1055
Msp20I TGGCCA 1 cut(s) 782
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 3 cut(s) 238, 551, 560
MspR9I CCNGG 2 cut(s) 32, 699
MvaI CCWGG 2 cut(s) 32, 699
MwoI GCNNNNNNNGC 1 cut(s) 851
NdeI CATATG 1 cut(s) 907
NdeII GATC 4 cut(s) 145, 179, 268, 556
NlaIII CATG 6 cut(s) 490, 539, 706, 717, 1027, 1060
NlaIV GGNNCC 2 cut(s) 423, 513
NmuCI GTSAC 3 cut(s) 121, 538, 847
NspI RCATGY 1 cut(s) 706
NspV TTCGAA 1 cut(s) 291
PaeI GCATGC 1 cut(s) 706
PaeR7I CTCGAG 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 575
PfeI GAWTC 4 cut(s) 58, 78, 400, 758
PkrI GCNGC 5 cut(s) 75, 328, 868, 967, 1029
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 30, 697
PspGI CCWGG 2 cut(s) 30, 697
PspN4I GGNNCC 2 cut(s) 423, 513
PspPI GGNCC 3 cut(s) 34, 503, 989
PstI CTGCAG 1 cut(s) 75
PsuI RGATCY 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 4 cut(s) 423, 583, 1011, 1035
RsaNI GTAC 4 cut(s) 422, 582, 1010, 1034
RseI CAYNNNNRTG 2 cut(s) 230, 1055
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 3 cut(s) 393, 747, 996
SatI GCNGC 5 cut(s) 74, 327, 867, 966, 1028
Sau3AI GATC 4 cut(s) 145, 179, 268, 556
Sau96I GGNCC 3 cut(s) 34, 503, 989
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 2 cut(s) 32, 699
SduI GDGCHC 4 cut(s) 47, 721, 916, 1072
SfaNI GCATC 2 cut(s) 828, 863
SfcI CTRYAG 2 cut(s) 71, 612
Sfr274I CTCGAG 1 cut(s) 330
SfuI TTCGAA 1 cut(s) 291
SinI GGWCC 2 cut(s) 34, 503
SlaI CTCGAG 1 cut(s) 330
SmiMI CAYNNNNRTG 2 cut(s) 230, 1055
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
SphI GCATGC 1 cut(s) 706
Sse9I AATT 8 cut(s) 17, 99, 256, 287, 601, 618, 708, 1046
SsiI CCGC 3 cut(s) 241, 326, 938
StyD4I CCNGG 2 cut(s) 30, 697
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 2 cut(s) 67, 595
TaiI ACGT 3 cut(s) 123, 174, 904
TaqI TCGA 8 cut(s) 81, 93, 174, 291, 319, 331, 647, 1053
TasI AATT 8 cut(s) 17, 99, 256, 287, 601, 618, 708, 1046
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 4 cut(s) 58, 78, 400, 758
Tru1I TTAA 3 cut(s) 393, 747, 996
Tru9I TTAA 3 cut(s) 393, 747, 996
TscAI CASTG 4 cut(s) 148, 729, 856, 967
TseFI GTSAC 3 cut(s) 121, 538, 847
TseI GCWGC 4 cut(s) 73, 866, 965, 1027
Tsp45I GTSAC 3 cut(s) 121, 538, 847
TspDTI ATGAA 5 cut(s) 214, 248, 803, 1020, 1039
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 4 cut(s) 148, 729, 856, 967
VpaK11BI GGWCC 2 cut(s) 34, 503
XapI RAATTY 5 cut(s) 99, 256, 287, 601, 708
XceI RCATGY 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 575
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.