pycom03g17920

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003786
Physical Location & Seq
Reverse (-)
19002276 .. 19003231
956 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGTATAGCAGGTGGTCAGAAGTAACCGAGGTGGTTCGTGCAAGAGAAGCCACATTGTGCACTCTCCACTCTCCACAACACACAAGCGCCCGAAAAGGAGCGTCGCTTTTACACTCTCTCCTCGCTATAAAACCCACCTTCACTCCCCTCCCTCTCCTCAGCCAATCCTCCCTCTCTCTCTCTCTCTCTCTTTCGGCATTTTTTACCACAAAGCTCACATCTTTCTCCAAAGCAGCCATGGCTCCGACGACGGCGAAGTCCCTATCCAAAACCGGCTCGGAGGACCCGAATCCGACGTCCAACGAGATCCGGTACCGGGGTGTCCGGAAGAGGCCTTGGGGACGCTACGCCGCCGAGATCAGAGATCCCGGGAAGAAGACCCGCGTCTGGCTTGGGACCTTCGACACCGCTGTGGACGCAGCGCGTGCGTACGACAAGGCGGCGCGTGAGTTTCGCGGAGGCAAGGCGAAGACCAACTTCCCCACCCCCTCTGAGCTCCAGCTCGACGCCGTCAACATCGTCAACATGAACAACGCCGCTAAATCAGCGGCGGCGACGAACGTCAGTTCGACCGCCCGGCTCCCATATCCGGCTCGGCCCACACCGACTACTCCGCCTCCTCAGTCGTCGACTTCGAGCGAAGCTCCCGCAACGCACGGCTCGATCTCGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009873 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010104 GO:0010105 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016604 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032870 GO:0033993 GO:0035556 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070297 GO:0070298 GO:0070887 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

225

Amino Acids

24.09

Weight (kDa)

10.27

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 104 - 153 1.4e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 256
AatII GACGTC 1 cut(s) 299
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 629
AccII CGCG 4 cut(s) 384, 424, 445, 456
AccIII TCCGGA 1 cut(s) 324
AclWI GGATC 2 cut(s) 301, 359
AcyI GRCGYC 2 cut(s) 296, 507
AdeI CACNNNGTG 1 cut(s) 57
AfaI GTAC 2 cut(s) 314, 431
AfiI CCNNNNNNNGG 3 cut(s) 316, 387, 589
AleI CACNNNNGTG 1 cut(s) 410
AluBI AGCT 4 cut(s) 214, 496, 502, 644
AluI AGCT 4 cut(s) 214, 496, 502, 644
Alw21I GWGCWC 2 cut(s) 62, 498
Alw44I GTGCAC 1 cut(s) 58
AlwI GGATC 2 cut(s) 301, 359
Ama87I CYCGRG 1 cut(s) 368
Aor13HI TCCGGA 1 cut(s) 324
AoxI GGCC 2 cut(s) 332, 596
ApaLI GTGCAC 1 cut(s) 58
ApeKI GCWGC 2 cut(s) 233, 419
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 3 cut(s) 89, 424, 445
AspS9I GGNCC 3 cut(s) 283, 396, 597
AsuC2I CCSGG 4 cut(s) 317, 369, 370, 577
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 283, 396
BaeGI GKGCMC 1 cut(s) 62
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 498
BbsI GAAGAC 2 cut(s) 383, 476
Bbv12I GWGCWC 2 cut(s) 62, 498
BbvCI CCTCAGC 1 cut(s) 158
BbvI GCAGC 2 cut(s) 245, 431
BceAI ACGGC 3 cut(s) 267, 494, 673
BcnI CCSGG 4 cut(s) 317, 369, 370, 577
BfoI RGCGCY 1 cut(s) 90
BisI GCNGC 7 cut(s) 234, 351, 420, 441, 537, 549, 552
BlsI GCNGC 7 cut(s) 235, 352, 421, 442, 538, 550, 553
Bme1390I CCNGG 4 cut(s) 317, 369, 370, 577
Bme18I GGWCC 2 cut(s) 283, 396
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 283, 396, 597
BmiI GGNNCC 5 cut(s) 243, 285, 314, 397, 581
BmrFI CCNGG 4 cut(s) 317, 369, 370, 577
BoxI GACNNNNGTC 2 cut(s) 383, 560
BpiI GAAGAC 2 cut(s) 383, 476
BplI GAGNNNNNCTC 2 cut(s) 628, 660
BpmI CTGGAG 1 cut(s) 482
Bpu10I CCTNAGC 1 cut(s) 158
BpuMI CCSGG 4 cut(s) 317, 369, 370, 577
BsaHI GRCGYC 2 cut(s) 296, 507
BsaJI CCNNGG 5 cut(s) 27, 237, 316, 335, 368
BsaWI WCCGGW 2 cut(s) 309, 324
BsaXI ACNNNNNCTCC 6 cut(s) 102, 127, 132, 157, 601, 631
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 387, 589
Bse118I RCCGGY 1 cut(s) 272
BseAI TCCGGA 1 cut(s) 324
BseDI CCNNGG 5 cut(s) 27, 237, 316, 335, 368
BseLI CCNNNNNNNGG 3 cut(s) 316, 387, 589
BseMII CTCAG 3 cut(s) 172, 483, 635
BseRI GAGGAG 3 cut(s) 110, 146, 609
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 2 cut(s) 245, 431
Bsh1236I CGCG 4 cut(s) 384, 424, 445, 456
Bsh1285I CGRYCG 1 cut(s) 573
BshFI GGCC 2 cut(s) 334, 598
BshNI GGYRCC 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 573
BsiHKAI GWGCWC 2 cut(s) 62, 498
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 7 cut(s) 273, 310, 316, 325, 369, 577, 590
BsiWI CGTACG 1 cut(s) 429
BslFI GGGAC 3 cut(s) 244, 354, 409
BslI CCNNNNNNNGG 3 cut(s) 316, 387, 589
BsmFI GGGAC 3 cut(s) 244, 354, 409
BsnI GGCC 2 cut(s) 334, 598
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 62, 498
Bsp13I TCCGGA 1 cut(s) 324
Bsp143I GATC 4 cut(s) 306, 357, 364, 663
Bsp19I CCATGG 1 cut(s) 237
BspANI GGCC 2 cut(s) 334, 598
BspCNI CTCAG 3 cut(s) 171, 484, 634
BspEI TCCGGA 1 cut(s) 324
BspFNI CGCG 4 cut(s) 384, 424, 445, 456
BspLI GGNNCC 5 cut(s) 243, 285, 314, 397, 581
BspPI GGATC 2 cut(s) 301, 359
BspT107I GGYRCC 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 5 cut(s) 27, 237, 316, 335, 368
BssMI GATC 4 cut(s) 306, 357, 364, 663
BssNI GRCGYC 2 cut(s) 296, 507
BssT1I CCWWGG 2 cut(s) 237, 335
Bst6I CTCTTC 1 cut(s) 323
BstACI GRCGYC 2 cut(s) 296, 507
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 3 cut(s) 158, 492, 621
BstDSI CCRYGG 1 cut(s) 237
BstFNI CGCG 4 cut(s) 384, 424, 445, 456
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 89, 424, 445
BstKTI GATC 4 cut(s) 309, 360, 367, 666
BstMBI GATC 4 cut(s) 306, 357, 364, 663
BstMCI CGRYCG 1 cut(s) 573
BstMWI GCNNNNNNNGC 5 cut(s) 47, 239, 416, 425, 545
BstPAI GACNNNNGTC 2 cut(s) 383, 560
BstSCI CCNGG 4 cut(s) 315, 367, 368, 575
BstSLI GKGCMC 1 cut(s) 62
BstUI CGCG 4 cut(s) 384, 424, 445, 456
BstV1I GCAGC 2 cut(s) 245, 431
BstV2I GAAGAC 2 cut(s) 383, 476
BstX2I RGATCY 2 cut(s) 306, 364
BstYI RGATCY 2 cut(s) 306, 364
BsuRI GGCC 2 cut(s) 334, 598
BtgI CCRYGG 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 3 cut(s) 89, 424, 445
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 3 cut(s) 283, 396, 597
Cfr9I CCCGGG 1 cut(s) 368
CseI GACGC 5 cut(s) 90, 351, 373, 425, 515
Csp6I GTAC 2 cut(s) 313, 430
CviAII CATG 2 cut(s) 238, 526
CviQI GTAC 2 cut(s) 313, 430
DdeI CTNAG 3 cut(s) 158, 492, 621
DpnI GATC 4 cut(s) 308, 359, 366, 665
DpnII GATC 4 cut(s) 306, 357, 364, 663
DraIII CACNNNGTG 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 256
DseDI GACNNNNNNGTC 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
EciI GGCGGA 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 496
Eco130I CCWWGG 2 cut(s) 237, 335
Eco147I AGGCCT 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 498
Eco47I GGWCC 2 cut(s) 283, 396
Eco53kI GAGCTC 1 cut(s) 496
Eco88I CYCGRG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 496
EcoO109I RGGNCCY 2 cut(s) 283, 396
EcoT14I CCWWGG 2 cut(s) 237, 335
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 2 cut(s) 237, 335
FaeI CATG 2 cut(s) 241, 529
FaiI YATR 5 cut(s) 6, 128, 239, 527, 586
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 3 cut(s) 244, 354, 409
FatI CATG 2 cut(s) 237, 525
FauI CCCGC 2 cut(s) 389, 655
FblI GTMKAC 1 cut(s) 629
Fnu4HI GCNGC 7 cut(s) 234, 351, 420, 441, 537, 549, 552
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 7 cut(s) 234, 351, 420, 441, 537, 549, 552
GlaI GCGC 3 cut(s) 88, 423, 444
GluI GCNGC 7 cut(s) 234, 351, 420, 441, 537, 549, 552
GsuI CTGGAG 1 cut(s) 482
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 2 cut(s) 334, 598
HapII CCGG 7 cut(s) 273, 310, 316, 325, 369, 577, 590
HgaI GACGC 5 cut(s) 90, 351, 373, 425, 515
HhaI GCGC 3 cut(s) 89, 424, 445
Hin1I GRCGYC 2 cut(s) 296, 507
Hin1II CATG 2 cut(s) 241, 529
Hin6I GCGC 3 cut(s) 87, 422, 443
HinP1I GCGC 3 cut(s) 87, 422, 443
HincII GTYRAC 3 cut(s) 514, 523, 630
HindII GTYRAC 3 cut(s) 514, 523, 630
HinfI GANTC 1 cut(s) 289
HpaII CCGG 7 cut(s) 273, 310, 316, 325, 369, 577, 590
Hpy166II GTNNAC 5 cut(s) 60, 415, 514, 523, 630
Hpy188I TCNGA 6 cut(s) 19, 246, 280, 294, 362, 493
Hpy188III TCNNGA 2 cut(s) 325, 667
Hpy8I GTNNAC 5 cut(s) 60, 415, 514, 523, 630
Hpy99I CGWCG 7 cut(s) 106, 250, 253, 298, 509, 559, 631
HpyAV CCTTC 2 cut(s) 148, 409
HpyCH4IV ACGT 2 cut(s) 296, 561
HpyCH4V TGCA 2 cut(s) 41, 60
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 239, 416, 425, 545
HpyF3I CTNAG 3 cut(s) 158, 492, 621
HpySE526I ACGT 2 cut(s) 296, 561
Hsp92I GRCGYC 2 cut(s) 296, 507
Hsp92II CATG 2 cut(s) 241, 529
HspAI GCGC 3 cut(s) 87, 422, 443
Kpn2I TCCGGA 1 cut(s) 324
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 4 cut(s) 306, 357, 364, 663
LmnI GCTCC 5 cut(s) 98, 247, 501, 585, 649
LpnPI CCDG 9 cut(s) 286, 323, 329, 338, 373, 382, 512, 590, 603
Lsp1109I GCAGC 2 cut(s) 245, 431
MaeII ACGT 2 cut(s) 296, 561
MaeIII GTNAC 1 cut(s) 22
MalI GATC 4 cut(s) 308, 359, 366, 665
MboI GATC 4 cut(s) 306, 357, 364, 663
MboII GAAGA 4 cut(s) 340, 385, 388, 481
MflI RGATCY 2 cut(s) 306, 364
MhlI GDGCHC 2 cut(s) 62, 498
MmeI TCCRAC 3 cut(s) 269, 317, 324
MroI TCCGGA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 2 cut(s) 410, 548
MspI CCGG 7 cut(s) 273, 310, 316, 325, 369, 577, 590
MspR9I CCNGG 4 cut(s) 317, 369, 370, 577
MvnI CGCG 4 cut(s) 384, 424, 445, 456
MwoI GCNNNNNNNGC 5 cut(s) 47, 239, 416, 425, 545
NciI CCSGG 4 cut(s) 317, 369, 370, 577
NcoI CCATGG 1 cut(s) 237
NdeII GATC 4 cut(s) 306, 357, 364, 663
NlaIII CATG 2 cut(s) 241, 529
NlaIV GGNNCC 5 cut(s) 243, 285, 314, 397, 581
NmeAIII GCCGAG 2 cut(s) 379, 574
OliI CACNNNNGTG 1 cut(s) 410
PceI AGGCCT 1 cut(s) 334
PcsI WCGNNNNNNNCGW 3 cut(s) 284, 632, 659
PfeI GAWTC 1 cut(s) 289
Pfl23II CGTACG 1 cut(s) 429
PflFI GACNNNGTC 1 cut(s) 509
PkrI GCNGC 7 cut(s) 235, 352, 421, 442, 538, 550, 553
PpuMI RGGWCCY 2 cut(s) 283, 396
PshAI GACNNNNGTC 2 cut(s) 383, 560
Psp124BI GAGCTC 1 cut(s) 498
Psp5II RGGWCCY 2 cut(s) 283, 396
PspLI CGTACG 1 cut(s) 429
PspN4I GGNNCC 5 cut(s) 243, 285, 314, 397, 581
PspPI GGNCC 3 cut(s) 283, 396, 597
PspPPI RGGWCCY 2 cut(s) 283, 396
PsuI RGATCY 2 cut(s) 306, 364
PsyI GACNNNGTC 1 cut(s) 509
RsaI GTAC 2 cut(s) 314, 431
RsaNI GTAC 2 cut(s) 313, 430
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 498
SalI GTCGAC 1 cut(s) 628
SatI GCNGC 7 cut(s) 234, 351, 420, 441, 537, 549, 552
Sau3AI GATC 4 cut(s) 306, 357, 364, 663
Sau96I GGNCC 3 cut(s) 283, 396, 597
ScrFI CCNGG 4 cut(s) 317, 369, 370, 577
SduI GDGCHC 2 cut(s) 62, 498
SinI GGWCC 2 cut(s) 283, 396
SmaI CCCGGG 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 410
SseBI AGGCCT 1 cut(s) 334
SstI GAGCTC 1 cut(s) 498
StuI AGGCCT 1 cut(s) 334
StyD4I CCNGG 4 cut(s) 315, 367, 368, 575
StyI CCWWGG 2 cut(s) 237, 335
TaiI ACGT 2 cut(s) 299, 564
TaqI TCGA 7 cut(s) 402, 504, 569, 629, 635, 662, 668
TauI GCSGC 5 cut(s) 353, 443, 539, 551, 554
TfiI GAWTC 1 cut(s) 289
TseI GCWGC 2 cut(s) 233, 419
TspDTI ATGAA 1 cut(s) 542
TspMI CCCGGG 1 cut(s) 368
Tth111I GACNNNGTC 1 cut(s) 509
VneI GTGCAC 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 283, 396
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XmaI CCCGGG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 629
ZraI GACGTC 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.