pycom03g21420

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002295
Physical Location & Seq
Forward (+)
21705744 .. 21712256
6513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1650 bp
ATGGAAAAAAAAACAGATCTCCCGGATCCCGTGCGCATCGTCAACGTCCTCATCACCTTCCCGCACTGCGACGAGTCAGGACACTCCAAAAAACGGCGTGCCACACTCTCTCACTCCCTCCCGATGCCACTTCCGCCGACCGCCCAAACCAGCGCCCTAATCTCGCCGGCGAATACGACGGCGAAAATGATGTCGTGGAACGGTGCCAAGGACCTGGTGATGACGTCTGACGACATTCCGTTCGGGACGGGGTCGTGGTATCTCTACGCCGGCGTCTCGTGCCTGCTCGTCCTCTTCGCCGGGATCATGTCCGGCCTCACCTTGGGGTTGATGTCACTCAACCTCGTCGACCTCGAAATCCTCCAGCGCAGCGGTAGCCCCACTGAGAAGAAGCAAGCCGCTAAGATACTACCTGTTGTGCAAAAGCAGCACCAACTTTTGGTCACGTTGCTTCTCTGTAATGCTTGTGCCATGGAGGCGCTTCCTTTATACCTTGATAAACTCTTTCACCCAGTTGTTGCTGTTGTGCTGTCTGTAACTTTTGTTCTGTTATTCGGGGAGATTATTCCGCAATCTATTTGCTCAAGATATGGGCTTTCGGTTGGTGCCAATTTCGTTTGGCTTGTCCGCATTCTGATGGCCATTTGTTATCCGATTGCATACCCAATTGGAAGGGTTCTTGATAAGGTACTTGGACATAGTGATGATTTGTTTAGGCGGGCTCAATTGAAGGCCCTAGTCTCTATTCATGGCCAAGAGGCTGGTAAGGGAGGTGAACTCACACATGATGAGACAACGATCATCAGTGGAGCCCTAGATTTAACTGAAAAGACCGCAGAGGAGGCTATGACACCTATTGAATCAACATTTTCCTTGGATGTTAATTCAAACTTAGACTGGGAAGCAATTGGGAAAATTCTTGCGCGAGGTCATAGTCGAGTCCCCATCTATTCTGAAAATCCAAAAAACATTATCGGCCTCCTTCTGGTGAAAAGTCTTCTCACAGTACGAGCAGAAGCAGAGATTCCGGTCAGTGCTGTTTCGATTCGAAGAATGCCTAGGGTTCCGGCAGATATGCCGCTTTATGATATACTTAATGAGTTTCAAAAAGGAAGCAGTCACCTGGCAGCTGTAGTGAAGAGCAAAGGAAAGAGCAACCTTCTGACAATTCCTGACGTAGAGAATTTCAAGGAGGATAAACTGGCACAGTTAAAATCTCAACTAGCTGCGTCCTTGCCAATCAAGCAAGATGACAAATCACGGAGTGTTGCTCTCGACATAGATAAAGCTCCAAAGTCTCTGACAAACAAGCCAACTGTTCAACCGAATGGTGCTATAAATCCAAATTCATCAGAGGACATTGAAGACGGGGAGGTCATAGGAATCATCACCCTGGAGGATGTTTTTGAAGAACTTCTGCAAGAGGAAATTGTAGATGAGACAGATGTATATATCGATGTACATAGGAGGATACGTGTGGCTGCTGCAGCAGCTGCTTCATCCATGGCACGAGCTCCATCAACTCGGAAGTTGCTTGTAGGCGCTCTAAGTAAGCACGGGCAAACCCCGAAGAAGTCTGCAGAAGATGATGGACATTCGGTGAGGTTTTCAGGGTCTCCACGGGAGCCTCTTCTAGGCGGTAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
DUF21

Protein Analysis

550

Amino Acids

59.64

Weight (kDa)

6.52

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 93 - 263 3.6e-34 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1373
AatII GACGTC 1 cut(s) 227
Acc16I TGCGCA 1 cut(s) 35
AccB1I GGYRCC 2 cut(s) 203, 605
AccB7I CCANNNNNTGG 1 cut(s) 439
AccI GTMKAC 1 cut(s) 348
AccII CGCG 1 cut(s) 925
AclWI GGATC 3 cut(s) 20, 33, 311
AcoI YGGCCR 2 cut(s) 639, 751
AcsI RAATTY 3 cut(s) 915, 1183, 1345
AcyI GRCGYC 2 cut(s) 224, 273
AdeI CACNNNGTG 1 cut(s) 1265
AfaI GTAC 3 cut(s) 690, 1008, 1461
AfiI CCNNNNNNNGG 5 cut(s) 93, 322, 439, 985, 1634
AflIII ACRYGT 1 cut(s) 1474
AgsI TTSAA 8 cut(s) 730, 860, 888, 1106, 1189, 1322, 1364, 1409
AjnI CCWGG 3 cut(s) 213, 1122, 1392
AluBI AGCT 5 cut(s) 1130, 1226, 1289, 1493, 1514
AluI AGCT 5 cut(s) 1130, 1226, 1289, 1493, 1514
Alw21I GWGCWC 1 cut(s) 1516
Alw26I GTCTC 6 cut(s) 280, 745, 785, 1302, 1433, 1620
AlwI GGATC 3 cut(s) 20, 33, 311
AlwNI CAGNNNCTG 1 cut(s) 1493
AoxI GGCC 5 cut(s) 313, 639, 732, 751, 976
ApeKI GCWGC 9 cut(s) 369, 427, 1127, 1226, 1481, 1484, 1487, 1490, 1493
ApoI RAATTY 3 cut(s) 915, 1183, 1345
ArsI GACNNNNNNTTYG 4 cut(s) 176, 208, 224, 256
Asp700I GAANNNNTTC 1 cut(s) 1413
AspA2I CCTAGG 1 cut(s) 1058
AspLEI GCGC 6 cut(s) 36, 155, 369, 481, 925, 1544
AspS9I GGNCC 2 cut(s) 211, 733
AsuC2I CCSGG 2 cut(s) 23, 301
AsuHPI GGTGA 9 cut(s) 46, 229, 310, 500, 785, 1000, 1112, 1381, 1612
AsuII TTCGAA 1 cut(s) 1048
AvaII GGWCC 1 cut(s) 211
AvrII CCTAGG 1 cut(s) 1058
BalI TGGCCA 2 cut(s) 641, 753
BamHI GGATCC 1 cut(s) 25
BanI GGYRCC 2 cut(s) 203, 605
BanII GRGCYC 3 cut(s) 724, 814, 1516
BauI CACGAG 2 cut(s) 277, 1509
BbsI GAAGAC 2 cut(s) 989, 1371
Bbv12I GWGCWC 1 cut(s) 1516
BbvI GCAGC 9 cut(s) 381, 439, 1139, 1213, 1468, 1471, 1480, 1499, 1502
BccI CCATC 4 cut(s) 631, 953, 1525, 1583
BceAI ACGGC 2 cut(s) 110, 195
BciT130I CCWGG 3 cut(s) 215, 1124, 1394
BciVI GTATCC 1 cut(s) 1464
BcnI CCSGG 2 cut(s) 23, 301
BcoDI GTCTC 6 cut(s) 280, 745, 785, 1302, 1433, 1620
BfaI CTAG 5 cut(s) 737, 815, 1059, 1223, 1634
BfmI CTRYAG 3 cut(s) 1131, 1485, 1578
BfoI RGCGCY 3 cut(s) 156, 482, 1545
BfuI GTATCC 1 cut(s) 1464
BglI GCCNNNNNGGC 1 cut(s) 476
BglII AGATCT 1 cut(s) 16
BlnI CCTAGG 1 cut(s) 1058
Bme1390I CCNGG 5 cut(s) 23, 215, 301, 1124, 1394
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 211, 733
BmiI GGNNCC 6 cut(s) 27, 205, 607, 811, 1065, 1626
BmrFI CCNGG 5 cut(s) 23, 215, 301, 1124, 1394
BmrI ACTGGG 2 cut(s) 506, 907
BmsI GCATC 2 cut(s) 45, 114
BmuI ACTGGG 2 cut(s) 506, 907
BpiI GAAGAC 2 cut(s) 989, 1371
BplI GAGNNNNNCTC 4 cut(s) 762, 794, 1255, 1287
BpmI CTGGAG 2 cut(s) 347, 1415
Bpu14I TTCGAA 1 cut(s) 1048
BpuEI CTTGAG 1 cut(s) 568
BpuMI CCSGG 2 cut(s) 23, 301
Bsa29I ATCGAT 1 cut(s) 1455
BsaAI YACGTR 1 cut(s) 1475
BsaHI GRCGYC 2 cut(s) 224, 273
BsaI GGTCTC 1 cut(s) 1620
BsaJI CCNNGG 8 cut(s) 207, 321, 471, 873, 1058, 1392, 1503, 1619
BsaWI WCCGGW 1 cut(s) 1027
BsaXI ACNNNNNCTCC 2 cut(s) 1459, 1489
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 322, 439, 985, 1634
Bse118I RCCGGY 2 cut(s) 166, 269
Bse1I ACTGG 3 cut(s) 512, 902, 1206
BseBI CCWGG 3 cut(s) 215, 1124, 1394
BseCI ATCGAT 1 cut(s) 1455
BseDI CCNNGG 8 cut(s) 207, 321, 471, 873, 1058, 1392, 1503, 1619
BseGI GGATG 3 cut(s) 883, 1405, 1499
BseLI CCNNNNNNNGG 5 cut(s) 93, 322, 439, 985, 1634
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 3 cut(s) 512, 902, 1206
BseRI GAGGAG 1 cut(s) 854
BseXI GCAGC 9 cut(s) 381, 439, 1139, 1213, 1468, 1471, 1480, 1499, 1502
Bsh1236I CGCG 1 cut(s) 925
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 5 cut(s) 315, 641, 734, 753, 978
BshNI GGYRCC 2 cut(s) 203, 605
BshVI ATCGAT 1 cut(s) 1455
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 1516
BsiSI CCGG 7 cut(s) 23, 167, 270, 300, 312, 1028, 1067
BslFI GGGAC 2 cut(s) 259, 926
BslI CCNNNNNNNGG 5 cut(s) 93, 322, 439, 985, 1634
BsmAI GTCTC 6 cut(s) 280, 745, 785, 1302, 1433, 1620
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 259, 926
BsmI GAATGC 2 cut(s) 630, 1059
BsnI GGCC 5 cut(s) 315, 641, 734, 753, 978
Bso31I GGTCTC 1 cut(s) 1620
Bsp119I TTCGAA 1 cut(s) 1048
Bsp1286I GDGCHC 3 cut(s) 724, 814, 1516
Bsp1407I TGTACA 1 cut(s) 1459
Bsp143I GATC 4 cut(s) 16, 25, 303, 798
Bsp19I CCATGG 2 cut(s) 471, 1503
BspANI GGCC 5 cut(s) 315, 641, 734, 753, 978
BspCNI CTCAG 1 cut(s) 376
BspDI ATCGAT 1 cut(s) 1455
BspFNI CGCG 1 cut(s) 925
BspLI GGNNCC 6 cut(s) 27, 205, 607, 811, 1065, 1626
BspMAI CTGCAG 2 cut(s) 1489, 1582
BspPI GGATC 3 cut(s) 20, 33, 311
BspQI GCTCTTC 1 cut(s) 1133
BspT104I TTCGAA 1 cut(s) 1048
BspT107I GGYRCC 2 cut(s) 203, 605
BspTNI GGTCTC 1 cut(s) 1620
BsrFI RCCGGY 2 cut(s) 166, 269
BsrGI TGTACA 1 cut(s) 1459
BsrI ACTGG 3 cut(s) 512, 902, 1206
BssAI RCCGGY 2 cut(s) 166, 269
BssECI CCNNGG 8 cut(s) 207, 321, 471, 873, 1058, 1392, 1503, 1619
BssMI GATC 4 cut(s) 16, 25, 303, 798
BssNI GRCGYC 2 cut(s) 224, 273
BssSI CACGAG 2 cut(s) 277, 1509
BssT1I CCWWGG 6 cut(s) 207, 321, 471, 873, 1058, 1503
Bst2BI CACGAG 2 cut(s) 277, 1509
Bst2UI CCWGG 3 cut(s) 215, 1124, 1394
Bst4CI ACNGT 4 cut(s) 203, 1006, 1209, 1318
Bst6I CTCTTC 3 cut(s) 299, 1133, 1635
BstACI GRCGYC 2 cut(s) 224, 273
BstAPI GCANNNNNTGC 1 cut(s) 1493
BstAUI TGTACA 1 cut(s) 1459
BstBAI YACGTR 1 cut(s) 1475
BstBI TTCGAA 1 cut(s) 1048
BstC8I GCNNGC 6 cut(s) 99, 168, 271, 284, 396, 720
BstDEI CTNAG 4 cut(s) 384, 402, 892, 1547
BstDSI CCRYGG 3 cut(s) 471, 1503, 1619
BstENI CCTNNNNNAGG 1 cut(s) 1632
BstF5I GGATG 3 cut(s) 883, 1405, 1499
BstFNI CGCG 1 cut(s) 925
BstH2I RGCGCY 3 cut(s) 156, 482, 1545
BstHHI GCGC 6 cut(s) 36, 155, 369, 481, 925, 1544
BstKTI GATC 4 cut(s) 19, 28, 306, 801
BstMAI GTCTC 6 cut(s) 280, 745, 785, 1302, 1433, 1620
BstMBI GATC 4 cut(s) 16, 25, 303, 798
BstMCI CGRYCG 1 cut(s) 141
BstNI CCWGG 3 cut(s) 215, 1124, 1394
BstSCI CCNGG 5 cut(s) 21, 213, 299, 1122, 1392
BstSFI CTRYAG 3 cut(s) 1131, 1485, 1578
BstUI CGCG 1 cut(s) 925
BstV1I GCAGC 9 cut(s) 381, 439, 1139, 1213, 1468, 1471, 1480, 1499, 1502
BstV2I GAAGAC 2 cut(s) 989, 1371
BstX2I RGATCY 2 cut(s) 16, 25
BstXI CCANNNNNNTGG 2 cut(s) 214, 761
BstYI RGATCY 2 cut(s) 16, 25
Bsu15I ATCGAT 1 cut(s) 1455
BsuI GTATCC 1 cut(s) 1464
BsuRI GGCC 5 cut(s) 315, 641, 734, 753, 978
BsuTUI ATCGAT 1 cut(s) 1455
BtgI CCRYGG 3 cut(s) 471, 1503, 1619
BtsCI GGATG 3 cut(s) 883, 1405, 1499
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 4 cut(s) 64, 381, 811, 1039
Cac8I GCNNGC 6 cut(s) 99, 168, 271, 284, 396, 720
CaiI CAGNNNCTG 1 cut(s) 1493
CfoI GCGC 6 cut(s) 36, 155, 369, 481, 925, 1544
Cfr10I RCCGGY 2 cut(s) 166, 269
Cfr13I GGNCC 2 cut(s) 211, 733
ClaI ATCGAT 1 cut(s) 1455
CseI GACGC 2 cut(s) 262, 1218
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 3 cut(s) 689, 1007, 1460
CviAII CATG 5 cut(s) 307, 472, 749, 785, 1504
CviQI GTAC 3 cut(s) 689, 1007, 1460
DdeI CTNAG 4 cut(s) 384, 402, 892, 1547
DpnI GATC 4 cut(s) 18, 27, 305, 800
DpnII GATC 4 cut(s) 16, 25, 303, 798
DraIII CACNNNGTG 1 cut(s) 1265
DrdI GACNNNNNNGTC 1 cut(s) 1373
DseDI GACNNNNNNGTC 1 cut(s) 1373
EaeI YGGCCR 2 cut(s) 639, 751
Eam1104I CTCTTC 3 cut(s) 299, 1133, 1635
EarI CTCTTC 3 cut(s) 299, 1133, 1635
EciI GGCGGA 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 1514
Eco130I CCWWGG 6 cut(s) 207, 321, 471, 873, 1058, 1503
Eco24I GRGCYC 3 cut(s) 724, 814, 1516
Eco31I GGTCTC 1 cut(s) 1620
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 1514
EcoICRI GAGCTC 1 cut(s) 1514
EcoNI CCTNNNNNAGG 1 cut(s) 1632
EcoO109I RGGNCCY 2 cut(s) 211, 733
EcoRII CCWGG 3 cut(s) 213, 1122, 1392
EcoT14I CCWWGG 6 cut(s) 207, 321, 471, 873, 1058, 1503
EcoT38I GRGCYC 3 cut(s) 724, 814, 1516
ErhI CCWWGG 6 cut(s) 207, 321, 471, 873, 1058, 1503
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 5 cut(s) 310, 475, 752, 788, 1507
FaqI GGGAC 2 cut(s) 259, 926
FatI CATG 5 cut(s) 306, 471, 748, 784, 1503
FauI CCCGC 2 cut(s) 69, 711
FblI GTMKAC 1 cut(s) 348
FokI GGATG 3 cut(s) 890, 1412, 1486
FriOI GRGCYC 3 cut(s) 724, 814, 1516
FspAI RTGCGCAY 1 cut(s) 35
FspBI CTAG 5 cut(s) 737, 815, 1059, 1223, 1634
FspI TGCGCA 1 cut(s) 35
GlaI GCGC 6 cut(s) 35, 154, 368, 480, 924, 1543
GsuI CTGGAG 2 cut(s) 347, 1415
HaeII RGCGCY 3 cut(s) 156, 482, 1545
HaeIII GGCC 5 cut(s) 315, 641, 734, 753, 978
HapII CCGG 7 cut(s) 23, 167, 270, 300, 312, 1028, 1067
HgaI GACGC 2 cut(s) 262, 1218
HhaI GCGC 6 cut(s) 36, 155, 369, 481, 925, 1544
Hin1I GRCGYC 2 cut(s) 224, 273
Hin1II CATG 5 cut(s) 310, 475, 752, 788, 1507
Hin6I GCGC 6 cut(s) 34, 153, 367, 479, 923, 1542
HinP1I GCGC 6 cut(s) 34, 153, 367, 479, 923, 1542
HincII GTYRAC 2 cut(s) 43, 349
HindII GTYRAC 2 cut(s) 43, 349
HinfI GANTC 6 cut(s) 74, 860, 939, 1024, 1045, 1383
HpaII CCGG 7 cut(s) 23, 167, 270, 300, 312, 1028, 1067
HphI GGTGA 9 cut(s) 46, 229, 310, 500, 785, 1000, 1112, 1381, 1612
Hpy166II GTNNAC 3 cut(s) 43, 349, 776
Hpy188I TCNGA 8 cut(s) 229, 636, 654, 955, 1164, 1302, 1354, 1527
Hpy188III TCNNGA 7 cut(s) 78, 121, 244, 585, 680, 1172, 1274
Hpy8I GTNNAC 3 cut(s) 43, 349, 776
Hpy99I CGWCG 3 cut(s) 74, 181, 350
HpyAV CCTTC 5 cut(s) 67, 666, 724, 992, 1169
HpyCH4III ACNGT 4 cut(s) 203, 1006, 1209, 1318
HpyCH4IV ACGT 5 cut(s) 45, 224, 446, 1176, 1474
HpyCH4V TGCA 5 cut(s) 421, 659, 1420, 1487, 1580
HpyF3I CTNAG 4 cut(s) 384, 402, 892, 1547
HpySE526I ACGT 5 cut(s) 45, 224, 446, 1176, 1474
Hsp92I GRCGYC 2 cut(s) 224, 273
Hsp92II CATG 5 cut(s) 310, 475, 752, 788, 1507
HspAI GCGC 6 cut(s) 34, 153, 367, 479, 923, 1542
KroI GCCGGC 2 cut(s) 166, 269
KroNI GCCGGC 2 cut(s) 168, 271
Kzo9I GATC 4 cut(s) 16, 25, 303, 798
LguI GCTCTTC 1 cut(s) 1133
LmnI GCTCC 4 cut(s) 809, 1294, 1519, 1624
Lsp1109I GCAGC 9 cut(s) 381, 439, 1139, 1213, 1468, 1471, 1480, 1499, 1502
LweI GCATC 2 cut(s) 45, 114
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 5 cut(s) 737, 815, 1059, 1223, 1634
MaeII ACGT 5 cut(s) 45, 224, 446, 1176, 1474
MaeIII GTNAC 4 cut(s) 333, 442, 535, 1118
MalI GATC 4 cut(s) 18, 27, 305, 800
MboI GATC 4 cut(s) 16, 25, 303, 798
MfeI CAATTG 3 cut(s) 666, 725, 906
MflI RGATCY 2 cut(s) 16, 25
MhlI GDGCHC 3 cut(s) 724, 814, 1516
MlsI TGGCCA 2 cut(s) 641, 753
MluNI TGGCCA 2 cut(s) 641, 753
MlyI GAGTC 2 cut(s) 83, 948
Mox20I TGGCCA 2 cut(s) 641, 753
MreI CGCCGGCG 2 cut(s) 166, 269
MroNI GCCGGC 2 cut(s) 166, 269
MroXI GAANNNNTTC 1 cut(s) 1413
MscI TGGCCA 2 cut(s) 641, 753
MseI TTAA 4 cut(s) 821, 882, 1095, 1211
MslI CAYNNNNRTG 2 cut(s) 635, 702
Msp20I TGGCCA 2 cut(s) 641, 753
MspA1I CMGCKG 3 cut(s) 372, 1130, 1493
MspI CCGG 7 cut(s) 23, 167, 270, 300, 312, 1028, 1067
MspR9I CCNGG 5 cut(s) 23, 215, 301, 1124, 1394
MunI CAATTG 3 cut(s) 666, 725, 906
Mva1269I GAATGC 2 cut(s) 630, 1059
MvaI CCWGG 3 cut(s) 215, 1124, 1394
MvnI CGCG 1 cut(s) 925
NaeI GCCGGC 2 cut(s) 168, 271
NciI CCSGG 2 cut(s) 23, 301
NcoI CCATGG 2 cut(s) 471, 1503
NdeII GATC 4 cut(s) 16, 25, 303, 798
NgoMIV GCCGGC 2 cut(s) 166, 269
NlaIII CATG 5 cut(s) 310, 475, 752, 788, 1507
NlaIV GGNNCC 6 cut(s) 27, 205, 607, 811, 1065, 1626
NmuCI GTSAC 3 cut(s) 333, 442, 1118
NsbI TGCGCA 1 cut(s) 35
NspV TTCGAA 1 cut(s) 1048
PciSI GCTCTTC 1 cut(s) 1133
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PctI GAATGC 2 cut(s) 630, 1059
PdiI GCCGGC 2 cut(s) 168, 271
PdmI GAANNNNTTC 1 cut(s) 1413
PfeI GAWTC 4 cut(s) 860, 1024, 1045, 1383
PflFI GACNNNGTC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 21
PleI GAGTC 2 cut(s) 82, 947
PpsI GAGTC 2 cut(s) 82, 947
Ppu21I YACGTR 1 cut(s) 1475
PpuMI RGGWCCY 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 1516
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 3 cut(s) 213, 1122, 1392
PspGI CCWGG 3 cut(s) 213, 1122, 1392
PspN4I GGNNCC 6 cut(s) 27, 205, 607, 811, 1065, 1626
PspPI GGNCC 2 cut(s) 211, 733
PspPPI RGGWCCY 1 cut(s) 211
PsrI GAACNNNNNNTAC 2 cut(s) 528, 560
PstI CTGCAG 2 cut(s) 1489, 1582
PstNI CAGNNNCTG 1 cut(s) 1493
PsuI RGATCY 2 cut(s) 16, 25
PsyI GACNNNGTC 1 cut(s) 250
PvuII CAGCTG 2 cut(s) 1130, 1493
RsaI GTAC 3 cut(s) 690, 1008, 1461
RsaNI GTAC 3 cut(s) 689, 1007, 1460
RseI CAYNNNNRTG 2 cut(s) 635, 702
SacI GAGCTC 1 cut(s) 1516
SalI GTCGAC 1 cut(s) 347
SapI GCTCTTC 1 cut(s) 1133
SaqAI TTAA 4 cut(s) 821, 882, 1095, 1211
Sau3AI GATC 4 cut(s) 16, 25, 303, 798
Sau96I GGNCC 2 cut(s) 211, 733
SchI GAGTC 2 cut(s) 83, 948
ScrFI CCNGG 5 cut(s) 23, 215, 301, 1124, 1394
SduI GDGCHC 3 cut(s) 724, 814, 1516
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 2 cut(s) 45, 114
SfcI CTRYAG 3 cut(s) 1131, 1485, 1578
SfuI TTCGAA 1 cut(s) 1048
SgrAI CRCCGGYG 2 cut(s) 166, 269
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 2 cut(s) 635, 702
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
SspMI CTAG 5 cut(s) 737, 815, 1059, 1223, 1634
SstI GAGCTC 1 cut(s) 1516
StyD4I CCNGG 5 cut(s) 21, 213, 299, 1122, 1392
StyI CCWWGG 6 cut(s) 207, 321, 471, 873, 1058, 1503
TaaI ACNGT 4 cut(s) 203, 1006, 1209, 1318
TaiI ACGT 5 cut(s) 48, 227, 449, 1179, 1477
TaqI TCGA 7 cut(s) 348, 354, 937, 1043, 1048, 1275, 1455
TatI WGTACW 1 cut(s) 1459
TauI GCSGC 2 cut(s) 401, 1081
TfiI GAWTC 4 cut(s) 860, 1024, 1045, 1383
Tru1I TTAA 4 cut(s) 821, 882, 1095, 1211
Tru9I TTAA 4 cut(s) 821, 882, 1095, 1211
TscAI CASTG 4 cut(s) 71, 388, 811, 1039
TseFI GTSAC 3 cut(s) 333, 442, 1118
TseI GCWGC 9 cut(s) 369, 427, 1127, 1226, 1481, 1484, 1487, 1490, 1493
Tsp45I GTSAC 3 cut(s) 333, 442, 1118
TspDTI ATGAA 3 cut(s) 737, 1338, 1488
TspGWI ACGGA 2 cut(s) 228, 1276
TspRI CASTG 4 cut(s) 71, 388, 811, 1039
Tth111I GACNNNGTC 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 211
XagI CCTNNNNNAGG 1 cut(s) 1632
XapI RAATTY 3 cut(s) 915, 1183, 1345
XmaJI CCTAGG 1 cut(s) 1058
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 1413
XspI CTAG 5 cut(s) 737, 815, 1059, 1223, 1634
ZraI GACGTC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.