pycom04g12680

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005832
Physical Location & Seq
Reverse (-)
15636859 .. 15637488
630 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12680.1

Sequence Viewer

Length: 630 bp
ATGATAATCCGGATGGGCGGCGGGCCCAGAACCTTCCCCGGCGGCGTATCCAAGTGGCAGTGGAAGCGCATGCAGGCGAAAAAAGCCAAGCAGCTCCTCAAGGCGCGCCTCTGCCGCGAGCGCCAAATCTACGAGATGCGAAAGAGGGCCGAGCTCAAAGCCGCCGTGTACGAGCTCGAGCGGCCGTGGGAGGTTGTTGAGAAAGCTCCGAACTTGTTCTCTGTCAGCGCCAACGAGCAAGTCAAGGTCTGGGCGGATAGGTTTCAGAAGCCTGGTGGGTTCGATTTGTGGACGGAGAGGGACGGCCCGCAGCTGTTTCATACCATGGAGGACTTACCCTCCGCGAGGTTTTTCCCCAAAGGGGTTGTTCATAGTGTCAGGCCGTATAAAAGGATTTCTGGGCCGGAGGAATTGGAGAGTTTGGAGCTTGAAGATGAAGATGAAGGATTTGGAGGAGCTGAGGAATTGGGAGATGGGTTTAAGGGTAAGGGGAAATTGGGAAGTGGGTCGTTCGATTTAGAGGAATCAGATGGGGAGGGTTTGAATCTGAAACATGCTCTGGAATCGTTGAGTGTGAATGGCCAAAGTGGGAAGAAGTACGATGGCAGAGTGAAAGAAGGGGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.62

Weight (kDa)

6.39

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 181
AccII CGCG 3 cut(s) 106, 117, 344
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 9 cut(s) 18, 21, 42, 115, 162, 181, 254, 308, 342
AcoI YGGCCR 2 cut(s) 182, 580
AfaI GTAC 2 cut(s) 170, 599
AfiI CCNNNNNNNGG 2 cut(s) 345, 361
AgsI TTSAA 2 cut(s) 431, 544
AjnI CCWGG 1 cut(s) 271
AluBI AGCT 7 cut(s) 94, 154, 175, 206, 313, 427, 458
AluI AGCT 7 cut(s) 94, 154, 175, 206, 313, 427, 458
Alw21I GWGCWC 2 cut(s) 156, 177
Ama87I CYCGRG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 7 cut(s) 23, 147, 182, 304, 380, 401, 580
ApaI GGGCCC 1 cut(s) 27
ApeKI GCWGC 2 cut(s) 91, 310
AscI GGCGCGCC 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 5 cut(s) 69, 106, 108, 123, 230
AspS9I GGNCC 5 cut(s) 23, 24, 147, 305, 401
AsuC2I CCSGG 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 582
BanII GRGCYC 3 cut(s) 27, 156, 177
Bbv12I GWGCWC 2 cut(s) 156, 177
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 2 cut(s) 103, 322
BccI CCATC 4 cut(s) 7, 467, 524, 596
BceAI ACGGC 4 cut(s) 149, 169, 319, 367
BciT130I CCWGG 1 cut(s) 273
BciVI GTATCC 1 cut(s) 58
BcnI CCSGG 1 cut(s) 39
BfoI RGCGCY 2 cut(s) 124, 231
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 7 cut(s) 19, 43, 92, 115, 162, 182, 311
BlsI GCNGC 7 cut(s) 20, 44, 93, 116, 163, 183, 312
Bme1390I CCNGG 2 cut(s) 39, 273
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 5 cut(s) 23, 24, 147, 305, 401
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 2 cut(s) 39, 273
BmsI GCATC 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 37, 185, 324
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 361
BseAI TCCGGA 1 cut(s) 9
BseBI CCWGG 1 cut(s) 273
BseDI CCNNGG 3 cut(s) 37, 185, 324
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 2 cut(s) 345, 361
BseMII CTCAG 1 cut(s) 450
BsePI GCGCGC 1 cut(s) 104
BseRI GAGGAG 2 cut(s) 86, 468
BseSI GKGCMC 1 cut(s) 27
BseX3I CGGCCG 1 cut(s) 182
BseXI GCAGC 2 cut(s) 103, 322
Bsh1236I CGCG 3 cut(s) 106, 117, 344
Bsh1285I CGRYCG 1 cut(s) 185
BshFI GGCC 7 cut(s) 25, 149, 184, 306, 382, 403, 582
BsiEI CGRYCG 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 156, 177
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 3 cut(s) 10, 39, 404
BslFI GGGAC 1 cut(s) 314
BslI CCNNNNNNNGG 2 cut(s) 345, 361
BsmFI GGGAC 1 cut(s) 314
BsnI GGCC 7 cut(s) 25, 149, 184, 306, 382, 403, 582
BsoBI CYCGRG 1 cut(s) 176
Bsp120I GGGCCC 1 cut(s) 23
Bsp1286I GDGCHC 3 cut(s) 27, 156, 177
Bsp13I TCCGGA 1 cut(s) 9
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 9 cut(s) 18, 21, 42, 115, 162, 181, 254, 308, 342
BspANI GGCC 7 cut(s) 25, 149, 184, 306, 382, 403, 582
BspCNI CTCAG 1 cut(s) 451
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 3 cut(s) 106, 117, 344
BspLI GGNNCC 1 cut(s) 25
BsrBI CCGCTC 1 cut(s) 181
BssECI CCNNGG 3 cut(s) 37, 185, 324
BssHII GCGCGC 1 cut(s) 104
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 273
BstC8I GCNNGC 6 cut(s) 23, 71, 75, 106, 119, 308
BstDEI CTNAG 1 cut(s) 459
BstDSI CCRYGG 2 cut(s) 185, 324
BstENI CCTNNNNNAGG 1 cut(s) 343
BstF5I GGATG 1 cut(s) 18
BstFNI CGCG 3 cut(s) 106, 117, 344
BstH2I RGCGCY 2 cut(s) 124, 231
BstHHI GCGC 5 cut(s) 69, 106, 108, 123, 230
BstMCI CGRYCG 1 cut(s) 185
BstMWI GCNNNNNNNGC 5 cut(s) 64, 83, 114, 120, 181
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 2 cut(s) 73, 557
BstSCI CCNGG 2 cut(s) 37, 271
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 3 cut(s) 106, 117, 344
BstV1I GCAGC 2 cut(s) 103, 322
BstZI CGGCCG 1 cut(s) 182
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 7 cut(s) 25, 149, 184, 306, 382, 403, 582
BtgI CCRYGG 2 cut(s) 185, 324
BtsCI GGATG 1 cut(s) 18
BtsI GCAGTG 1 cut(s) 65
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 6 cut(s) 23, 71, 75, 106, 119, 308
CfoI GCGC 5 cut(s) 69, 106, 108, 123, 230
Cfr13I GGNCC 5 cut(s) 23, 24, 147, 305, 401
Csp6I GTAC 2 cut(s) 169, 598
CviAII CATG 3 cut(s) 70, 325, 554
CviQI GTAC 2 cut(s) 169, 598
DdeI CTNAG 1 cut(s) 459
EaeI YGGCCR 2 cut(s) 182, 580
EagI CGGCCG 1 cut(s) 182
EciI GGCGGA 1 cut(s) 269
Ecl136II GAGCTC 2 cut(s) 154, 175
EclXI CGGCCG 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 3 cut(s) 27, 156, 177
Eco52I CGGCCG 1 cut(s) 182
Eco53kI GAGCTC 2 cut(s) 154, 175
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 2 cut(s) 154, 175
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 324
EcoT38I GRGCYC 3 cut(s) 27, 156, 177
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 3 cut(s) 73, 328, 557
FaiI YATR 6 cut(s) 71, 321, 326, 372, 387, 555
FaqI GGGAC 1 cut(s) 314
FatI CATG 3 cut(s) 69, 324, 553
FauI CCCGC 2 cut(s) 14, 315
Fnu4HI GCNGC 7 cut(s) 19, 43, 92, 115, 162, 182, 311
FokI GGATG 1 cut(s) 25
FriOI GRGCYC 3 cut(s) 27, 156, 177
Fsp4HI GCNGC 7 cut(s) 19, 43, 92, 115, 162, 182, 311
GlaI GCGC 5 cut(s) 68, 105, 107, 122, 229
GluI GCNGC 7 cut(s) 19, 43, 92, 115, 162, 182, 311
HaeII RGCGCY 2 cut(s) 124, 231
HaeIII GGCC 7 cut(s) 25, 149, 184, 306, 382, 403, 582
HapII CCGG 3 cut(s) 10, 39, 404
HhaI GCGC 5 cut(s) 69, 106, 108, 123, 230
Hin1II CATG 3 cut(s) 73, 328, 557
Hin6I GCGC 5 cut(s) 67, 104, 106, 121, 228
HinP1I GCGC 5 cut(s) 67, 104, 106, 121, 228
HinfI GANTC 3 cut(s) 524, 544, 563
HpaII CCGG 3 cut(s) 10, 39, 404
Hpy166II GTNNAC 2 cut(s) 169, 291
Hpy188I TCNGA 4 cut(s) 210, 267, 529, 549
Hpy188III TCNNGA 2 cut(s) 10, 560
Hpy8I GTNNAC 2 cut(s) 169, 291
HpyAV CCTTC 3 cut(s) 43, 437, 611
HpyCH4V TGCA 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 83, 114, 120, 181
HpyF3I CTNAG 1 cut(s) 459
Hsp92II CATG 3 cut(s) 73, 328, 557
HspAI GCGC 5 cut(s) 67, 104, 106, 121, 228
Kpn2I TCCGGA 1 cut(s) 9
LmnI GCTCC 4 cut(s) 99, 211, 424, 455
Lsp1109I GCAGC 2 cut(s) 103, 322
LweI GCATC 1 cut(s) 126
MbiI CCGCTC 1 cut(s) 181
MboII GAAGA 3 cut(s) 443, 449, 604
MhlI GDGCHC 3 cut(s) 27, 156, 177
MlsI TGGCCA 1 cut(s) 582
MluCI AATT 3 cut(s) 410, 464, 494
MluNI TGGCCA 1 cut(s) 582
Mox20I TGGCCA 1 cut(s) 582
MroI TCCGGA 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 215
MscI TGGCCA 1 cut(s) 582
MseI TTAA 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 582
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 3 cut(s) 10, 39, 404
MspR9I CCNGG 2 cut(s) 39, 273
MvaI CCWGG 1 cut(s) 273
MvnI CGCG 3 cut(s) 106, 117, 344
MwoI GCNNNNNNNGC 5 cut(s) 64, 83, 114, 120, 181
NciI CCSGG 1 cut(s) 39
NcoI CCATGG 1 cut(s) 324
NlaIII CATG 3 cut(s) 73, 328, 557
NlaIV GGNNCC 1 cut(s) 25
NmeAIII GCCGAG 1 cut(s) 175
NspI RCATGY 2 cut(s) 73, 557
PaeI GCATGC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 176
PalAI GGCGCGCC 1 cut(s) 104
PauI GCGCGC 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 3 cut(s) 524, 544, 563
PkrI GCNGC 7 cut(s) 20, 44, 93, 116, 163, 183, 312
Psp124BI GAGCTC 2 cut(s) 156, 177
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 25
PspOMI GGGCCC 1 cut(s) 23
PspPI GGNCC 5 cut(s) 23, 24, 147, 305, 401
PspXI VCTCGAGB 1 cut(s) 176
PteI GCGCGC 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 2 cut(s) 170, 599
RsaNI GTAC 2 cut(s) 169, 598
SacI GAGCTC 2 cut(s) 156, 177
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 7 cut(s) 19, 43, 92, 115, 162, 182, 311
Sau96I GGNCC 5 cut(s) 23, 24, 147, 305, 401
ScrFI CCNGG 2 cut(s) 39, 273
SduI GDGCHC 3 cut(s) 27, 156, 177
SfaNI GCATC 1 cut(s) 126
Sfr274I CTCGAG 1 cut(s) 176
SgsI GGCGCGCC 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 98, 176
SmoI CTYRAG 2 cut(s) 98, 176
SphI GCATGC 1 cut(s) 73
Sse9I AATT 3 cut(s) 410, 464, 494
SsiI CCGC 9 cut(s) 18, 21, 42, 115, 162, 181, 254, 308, 342
SstI GAGCTC 2 cut(s) 156, 177
StyD4I CCNGG 2 cut(s) 37, 271
StyI CCWWGG 1 cut(s) 324
TaqI TCGA 3 cut(s) 177, 282, 513
TasI AATT 3 cut(s) 410, 464, 494
TauI GCSGC 5 cut(s) 21, 45, 117, 164, 184
TfiI GAWTC 3 cut(s) 524, 544, 563
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 1 cut(s) 65
TseI GCWGC 2 cut(s) 91, 310
TspDTI ATGAA 4 cut(s) 308, 359, 450, 456
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 65
XagI CCTNNNNNAGG 1 cut(s) 343
XceI RCATGY 2 cut(s) 73, 557
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.