Rmu_sc0002031.1_g000002

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002031.1
OG0005832
Physical Location & Seq
Reverse (-)
3413 .. 4096
684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002031.1_g000002.1.cds

Sequence Viewer

Length: 684 bp
atgggcggcgggcccaggaccttccccggcggcgtatccaagtggcagtggaagcgcatgcaggccaagaaagccaagcagctcctcaaggcccgcctctgccgcgagcgccaaatctacgagatgcgaaagcgggccgagctcaaggccgccgtgtcggagctcgagcgcccctgggagaccgtcgagaagcctcccaatttgttctccgtcagcgccgacgagcaggtgaaggtgttggccgatcggttccagaggccgggagggtttgacctgtggaccgagagggatgggccggagctgttccggaccatggaggacctgccgtcggcgaggttttttcccaagggggttgtgcatagtgtgaggccgtataggagggttggggctgaggaattgggggaggaggaagatgagggttttggaggagtcaagggattggttgaggggcttaagggtgcagagaggtcaaatggggagggtttgaattcgaaaagttgttcggaggcggtgaattttcgaaatgggaggatgtataatggtaaactgaggaggaaggggaaagggaataggggaaatgctgaagcttcaagtgatgttgataggaaacagagaggacagagtcctgtaatgcgcagtggcgaaaatgggaattctaggagagaggaaggggaaagggaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.63

Weight (kDa)

9.8

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc16I TGCGCA 1 cut(s) 635
Acc36I ACCTGC 2 cut(s) 217, 330
AccII CGCG 1 cut(s) 105
AccIII TCCGGA 1 cut(s) 306
AciI CCGC 8 cut(s) 6, 9, 30, 94, 103, 133, 150, 509
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 3 cut(s) 487, 514, 652
AcuI CTGAAG 1 cut(s) 603
AfiI CCNNNNNNNGG 3 cut(s) 259, 313, 328
AflII CTTAAG 1 cut(s) 452
AgsI TTSAA 2 cut(s) 487, 591
AhdI GACNNNNNGTC 1 cut(s) 325
AjnI CCWGG 2 cut(s) 14, 173
AluBI AGCT 5 cut(s) 82, 142, 163, 301, 587
AluI AGCT 5 cut(s) 82, 142, 163, 301, 587
Alw21I GWGCWC 2 cut(s) 144, 165
Alw26I GTCTC 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 164
Aor13HI TCCGGA 1 cut(s) 306
AoxI GGCC 9 cut(s) 11, 63, 90, 135, 147, 240, 257, 293, 368
ApaI GGGCCC 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 79
ApoI RAATTY 3 cut(s) 487, 514, 652
AspLEI GCGC 5 cut(s) 57, 111, 171, 218, 636
AspS9I GGNCC 9 cut(s) 11, 12, 18, 91, 135, 279, 293, 309, 319
AsuC2I CCSGG 2 cut(s) 27, 261
AsuHPI GGTGA 2 cut(s) 241, 523
AsuII TTCGAA 2 cut(s) 491, 520
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 4 cut(s) 18, 279, 309, 319
BaeGI GKGCMC 1 cut(s) 15
BanII GRGCYC 3 cut(s) 15, 144, 165
Bbv12I GWGCWC 2 cut(s) 144, 165
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 91
BccI CCATC 1 cut(s) 284
BceAI ACGGC 3 cut(s) 137, 310, 355
BciT130I CCWGG 2 cut(s) 16, 175
BciVI GTATCC 1 cut(s) 46
BcnI CCSGG 2 cut(s) 27, 261
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 1 cut(s) 657
BfoI RGCGCY 3 cut(s) 112, 172, 219
BfrI CTTAAG 1 cut(s) 452
BfuAI ACCTGC 2 cut(s) 217, 330
BfuI GTATCC 1 cut(s) 46
BisI GCNGC 5 cut(s) 7, 31, 80, 103, 150
BlsI GCNGC 5 cut(s) 8, 32, 81, 104, 151
Bme1390I CCNGG 4 cut(s) 16, 27, 175, 261
Bme18I GGWCC 4 cut(s) 18, 279, 309, 319
BmeRI GACNNNNNGTC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 9 cut(s) 11, 12, 18, 91, 135, 279, 293, 309, 319
BmiI GGNNCC 2 cut(s) 13, 251
BmrFI CCNGG 4 cut(s) 16, 27, 175, 261
BmsI GCATC 1 cut(s) 114
Bpu10I CCTNAGC 1 cut(s) 390
Bpu14I TTCGAA 2 cut(s) 491, 520
BpuEI CTTGAG 2 cut(s) 71, 128
BpuMI CCSGG 2 cut(s) 27, 261
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 6 cut(s) 14, 25, 173, 174, 312, 345
BsaWI WCCGGW 1 cut(s) 306
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 313, 328
BseAI TCCGGA 1 cut(s) 306
BseBI CCWGG 2 cut(s) 16, 175
BseDI CCNNGG 6 cut(s) 14, 25, 173, 174, 312, 345
BseGI GGATG 2 cut(s) 295, 537
BseLI CCNNNNNNNGG 3 cut(s) 259, 313, 328
BseMII CTCAG 2 cut(s) 381, 539
BseRI GAGGAG 4 cut(s) 74, 419, 441, 565
BseSI GKGCMC 1 cut(s) 15
BseXI GCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 105
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 9 cut(s) 13, 65, 92, 137, 149, 242, 259, 295, 370
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 2 cut(s) 144, 165
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 4 cut(s) 27, 260, 296, 307
BslI CCNNNNNNNGG 3 cut(s) 259, 313, 328
BsmAI GTCTC 1 cut(s) 173
BsnI GGCC 9 cut(s) 13, 65, 92, 137, 149, 242, 259, 295, 370
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 164
Bsp119I TTCGAA 2 cut(s) 491, 520
Bsp120I GGGCCC 1 cut(s) 11
Bsp1286I GDGCHC 3 cut(s) 15, 144, 165
Bsp13I TCCGGA 1 cut(s) 306
Bsp143I GATC 1 cut(s) 244
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 8 cut(s) 6, 9, 30, 94, 103, 133, 150, 509
BspANI GGCC 9 cut(s) 13, 65, 92, 137, 149, 242, 259, 295, 370
BspCNI CTCAG 2 cut(s) 382, 540
BspEI TCCGGA 1 cut(s) 306
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 13, 251
BspMI ACCTGC 2 cut(s) 217, 330
BspT104I TTCGAA 2 cut(s) 491, 520
BspTI CTTAAG 1 cut(s) 452
BspTNI GGTCTC 1 cut(s) 173
BssECI CCNNGG 6 cut(s) 14, 25, 173, 174, 312, 345
BssMI GATC 1 cut(s) 244
BssT1I CCWWGG 2 cut(s) 312, 345
Bst2UI CCWGG 2 cut(s) 16, 175
Bst4CI ACNGT 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 452
BstBI TTCGAA 2 cut(s) 491, 520
BstC8I GCNNGC 6 cut(s) 11, 59, 63, 94, 107, 135
BstDEI CTNAG 2 cut(s) 390, 548
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 2 cut(s) 295, 537
BstFNI CGCG 1 cut(s) 105
BstH2I RGCGCY 3 cut(s) 112, 172, 219
BstHHI GCGC 5 cut(s) 57, 111, 171, 218, 636
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 1 cut(s) 244
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 5 cut(s) 52, 71, 102, 108, 139
BstNI CCWGG 2 cut(s) 16, 175
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 4 cut(s) 14, 25, 173, 259
BstSLI GKGCMC 1 cut(s) 15
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 91
BsuI GTATCC 1 cut(s) 46
BsuRI GGCC 9 cut(s) 13, 65, 92, 137, 149, 242, 259, 295, 370
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 295, 537
BtsI GCAGTG 2 cut(s) 53, 643
BtsIMutI CAGTG 2 cut(s) 53, 643
BveI ACCTGC 2 cut(s) 217, 330
Cac8I GCNNGC 6 cut(s) 11, 59, 63, 94, 107, 135
CfoI GCGC 5 cut(s) 57, 111, 171, 218, 636
Cfr13I GGNCC 9 cut(s) 11, 12, 18, 91, 135, 279, 293, 309, 319
CviAII CATG 2 cut(s) 58, 313
DdeI CTNAG 2 cut(s) 390, 548
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
DriI GACNNNNNGTC 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 240
Eam1105I GACNNNNNGTC 1 cut(s) 325
Ecl136II GAGCTC 2 cut(s) 142, 163
Eco130I CCWWGG 2 cut(s) 312, 345
Eco24I GRGCYC 3 cut(s) 15, 144, 165
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 4 cut(s) 18, 279, 309, 319
Eco53kI GAGCTC 2 cut(s) 142, 163
Eco57I CTGAAG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 164
EcoICRI GAGCTC 2 cut(s) 142, 163
EcoO109I RGGNCCY 2 cut(s) 18, 319
EcoRI GAATTC 2 cut(s) 487, 652
EcoRII CCWGG 2 cut(s) 14, 173
EcoT14I CCWWGG 2 cut(s) 312, 345
EcoT38I GRGCYC 3 cut(s) 15, 144, 165
ErhI CCWWGG 2 cut(s) 312, 345
FaeI CATG 2 cut(s) 61, 316
FaiI YATR 5 cut(s) 59, 314, 360, 375, 537
FatI CATG 2 cut(s) 57, 312
FauI CCCGC 3 cut(s) 2, 101, 126
Fnu4HI GCNGC 5 cut(s) 7, 31, 80, 103, 150
FokI GGATG 2 cut(s) 302, 544
FriOI GRGCYC 3 cut(s) 15, 144, 165
Fsp4HI GCNGC 5 cut(s) 7, 31, 80, 103, 150
FspBI CTAG 1 cut(s) 657
FspI TGCGCA 1 cut(s) 635
GlaI GCGC 5 cut(s) 56, 110, 170, 217, 635
GluI GCNGC 5 cut(s) 7, 31, 80, 103, 150
HaeII RGCGCY 3 cut(s) 112, 172, 219
HaeIII GGCC 9 cut(s) 13, 65, 92, 137, 149, 242, 259, 295, 370
HapII CCGG 4 cut(s) 27, 260, 296, 307
HhaI GCGC 5 cut(s) 57, 111, 171, 218, 636
Hin1II CATG 2 cut(s) 61, 316
Hin6I GCGC 5 cut(s) 55, 109, 169, 216, 634
HinP1I GCGC 5 cut(s) 55, 109, 169, 216, 634
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 2 cut(s) 429, 622
HpaII CCGG 4 cut(s) 27, 260, 296, 307
HphI GGTGA 2 cut(s) 241, 523
Hpy166II GTNNAC 2 cut(s) 279, 545
Hpy188I TCNGA 2 cut(s) 160, 505
Hpy188III TCNNGA 3 cut(s) 187, 253, 307
Hpy8I GTNNAC 2 cut(s) 279, 545
Hpy99I CGWCG 3 cut(s) 188, 224, 331
HpyAV CCTTC 4 cut(s) 31, 226, 550, 662
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 61, 358, 461
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 71, 102, 108, 139
HpyF3I CTNAG 2 cut(s) 390, 548
Hsp92II CATG 2 cut(s) 61, 316
HspAI GCGC 5 cut(s) 55, 109, 169, 216, 634
Kpn2I TCCGGA 1 cut(s) 306
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 3 cut(s) 87, 160, 298
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 1 cut(s) 114
MaeI CTAG 1 cut(s) 657
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 1 cut(s) 422
MhlI GDGCHC 3 cut(s) 15, 144, 165
MluCI AATT 5 cut(s) 199, 395, 487, 514, 652
MlyI GAGTC 2 cut(s) 438, 631
MmeI TCCRAC 1 cut(s) 138
MroI TCCGGA 1 cut(s) 306
MseI TTAA 1 cut(s) 453
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 4 cut(s) 27, 260, 296, 307
MspR9I CCNGG 4 cut(s) 16, 27, 175, 261
MvaI CCWGG 2 cut(s) 16, 175
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 5 cut(s) 52, 71, 102, 108, 139
NciI CCSGG 2 cut(s) 27, 261
NcoI CCATGG 1 cut(s) 312
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 61, 316
NlaIV GGNNCC 2 cut(s) 13, 251
NmeAIII GCCGAG 1 cut(s) 163
NsbI TGCGCA 1 cut(s) 635
NspI RCATGY 1 cut(s) 61
NspV TTCGAA 2 cut(s) 491, 520
PaeI GCATGC 1 cut(s) 61
PaeR7I CTCGAG 1 cut(s) 164
PaqCI CACCTGC 1 cut(s) 217
PasI CCCWGGG 1 cut(s) 174
PflFI GACNNNGTC 1 cut(s) 621
PkrI GCNGC 5 cut(s) 8, 32, 81, 104, 151
Ple19I CGATCG 1 cut(s) 247
PleI GAGTC 2 cut(s) 437, 630
PpsI GAGTC 2 cut(s) 437, 630
PpuMI RGGWCCY 2 cut(s) 18, 319
Psp124BI GAGCTC 2 cut(s) 144, 165
Psp5II RGGWCCY 2 cut(s) 18, 319
Psp6I CCWGG 2 cut(s) 14, 173
PspGI CCWGG 2 cut(s) 14, 173
PspN4I GGNNCC 2 cut(s) 13, 251
PspOMI GGGCCC 1 cut(s) 11
PspPI GGNCC 9 cut(s) 11, 12, 18, 91, 135, 279, 293, 309, 319
PspPPI RGGWCCY 2 cut(s) 18, 319
PspXI VCTCGAGB 1 cut(s) 164
PsyI GACNNNGTC 1 cut(s) 621
PvuI CGATCG 1 cut(s) 247
SacI GAGCTC 2 cut(s) 144, 165
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 5 cut(s) 7, 31, 80, 103, 150
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 9 cut(s) 11, 12, 18, 91, 135, 279, 293, 309, 319
SchI GAGTC 2 cut(s) 438, 631
ScrFI CCNGG 4 cut(s) 16, 27, 175, 261
SduI GDGCHC 3 cut(s) 15, 144, 165
SfaNI GCATC 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 164
SfuI TTCGAA 2 cut(s) 491, 520
SinI GGWCC 4 cut(s) 18, 279, 309, 319
SlaI CTCGAG 1 cut(s) 164
SmlI CTYRAG 4 cut(s) 86, 143, 164, 452
SmoI CTYRAG 4 cut(s) 86, 143, 164, 452
SphI GCATGC 1 cut(s) 61
Sse9I AATT 5 cut(s) 199, 395, 487, 514, 652
SsiI CCGC 8 cut(s) 6, 9, 30, 94, 103, 133, 150, 509
SspMI CTAG 1 cut(s) 657
SstI GAGCTC 2 cut(s) 144, 165
StyD4I CCNGG 4 cut(s) 14, 25, 173, 259
StyI CCWWGG 2 cut(s) 312, 345
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 4 cut(s) 165, 186, 491, 520
TaqII GACCGA 1 cut(s) 296
TasI AATT 5 cut(s) 199, 395, 487, 514, 652
TauI GCSGC 4 cut(s) 9, 33, 105, 152
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 2 cut(s) 53, 643
TseI GCWGC 1 cut(s) 79
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 2 cut(s) 53, 643
Tth111I GACNNNGTC 1 cut(s) 621
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 4 cut(s) 18, 279, 309, 319
XapI RAATTY 3 cut(s) 487, 514, 652
XceI RCATGY 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 164
XspI CTAG 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.