Rroxscaffold_2G00085440

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005832
Physical Location & Seq
Forward (+)
7757017 .. 7758538
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00085440.1

Sequence Viewer

Length: 453 bp
ATGGGCGGCGGGCCCGTGCTTCCCGGTGGCGTATCCAAGTGGCGGTGGAAGCGCATGCATGCCAAGAAAGCCAAGCAACTCCTCAAGGCCCGCCTCGTCGCGAGTGCCAAATCTACAAGATGCGAAAGCGGGCCCATCTCAAAGCCACCGTGTCGGAGCTTGAGCGCACACGGGAGACCGTCGAGAAGCCTCCCAATTTGTTCTCCGTCGGCCGATGAGCAAGTTAAGGTCTTGGCCGATAGGTTCCGGAGGCGGGAGAAGGAGAGAAGATGGTCGTGGTTTTGGAGATCGAGAGAGAGAGGATCCAAAAAGAAACTTAGATCGAAAGACCAGTTTACGGAACAATTGGTTCCGGAAAGATACTCATTGTTCAAGCCTAGTGTCAACAAACTTGTTGTTCTCCCTAATTCTTCGAGTCAGCAAATCATCAGCCAGAATCAGACATCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

17.17

Weight (kDa)

11.28

Isoelectric Point (pI)

75.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 101
AccIII TCCGGA 2 cut(s) 246, 352
AciI CCGC 6 cut(s) 6, 9, 43, 91, 129, 253
AclWI GGATC 2 cut(s) 297, 310
AcoI YGGCCR 2 cut(s) 210, 234
AfiI CCNNNNNNNGG 3 cut(s) 42, 253, 337
AgsI TTSAA 1 cut(s) 373
AluBI AGCT 1 cut(s) 159
AluI AGCT 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 2 cut(s) 297, 310
Aor13HI TCCGGA 2 cut(s) 246, 352
AoxI GGCC 5 cut(s) 11, 87, 131, 210, 234
ApaI GGGCCC 2 cut(s) 15, 135
AspLEI GCGC 2 cut(s) 54, 167
AspS9I GGNCC 5 cut(s) 11, 12, 88, 131, 132
AsuC2I CCSGG 1 cut(s) 24
BaeGI GKGCMC 2 cut(s) 15, 135
BamHI GGATCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 15, 135
BccI CCATC 2 cut(s) 143, 264
BciVI GTATCC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 378
BfuI GTATCC 1 cut(s) 43
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 24
BmgT120I GGNCC 5 cut(s) 11, 12, 88, 131, 132
BmiI GGNNCC 5 cut(s) 13, 133, 245, 304, 351
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 1 cut(s) 110
BpuEI CTTGAG 2 cut(s) 68, 181
BpuMI CCSGG 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 169
BsaWI WCCGGW 2 cut(s) 246, 352
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 253, 337
Bse1I ACTGG 1 cut(s) 331
BseAI TCCGGA 2 cut(s) 246, 352
BseLI CCNNNNNNNGG 3 cut(s) 42, 253, 337
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 1 cut(s) 331
BseRI GAGGAG 1 cut(s) 71
BseSI GKGCMC 2 cut(s) 15, 135
BseX3I CGGCCG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 5 cut(s) 13, 89, 133, 212, 236
BsiEI CGRYCG 1 cut(s) 213
BsiSI CCGG 3 cut(s) 24, 247, 353
BslI CCNNNNNNNGG 3 cut(s) 42, 253, 337
BsmAI GTCTC 1 cut(s) 169
BsnI GGCC 5 cut(s) 13, 89, 133, 212, 236
Bso31I GGTCTC 1 cut(s) 169
Bsp120I GGGCCC 2 cut(s) 11, 131
Bsp1286I GDGCHC 2 cut(s) 15, 135
Bsp13I TCCGGA 2 cut(s) 246, 352
Bsp143I GATC 3 cut(s) 287, 302, 320
Bsp68I TCGCGA 1 cut(s) 101
BspACI CCGC 6 cut(s) 6, 9, 43, 91, 129, 253
BspANI GGCC 5 cut(s) 13, 89, 133, 212, 236
BspCNI CTCAG 1 cut(s) 439
BspEI TCCGGA 2 cut(s) 246, 352
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 5 cut(s) 13, 133, 245, 304, 351
BspPI GGATC 2 cut(s) 297, 310
BspTNI GGTCTC 1 cut(s) 169
BsrI ACTGG 1 cut(s) 331
BssMI GATC 3 cut(s) 287, 302, 320
Bst4CI ACNGT 2 cut(s) 150, 180
BstC8I GCNNGC 5 cut(s) 11, 56, 60, 91, 131
BstDEI CTNAG 2 cut(s) 317, 447
BstFNI CGCG 1 cut(s) 101
BstHHI GCGC 2 cut(s) 54, 167
BstKTI GATC 3 cut(s) 290, 305, 323
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 3 cut(s) 287, 302, 320
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 2 cut(s) 49, 68
BstNSI RCATGY 2 cut(s) 58, 62
BstSCI CCNGG 1 cut(s) 22
BstSLI GKGCMC 2 cut(s) 15, 135
BstUI CGCG 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BstZI CGGCCG 1 cut(s) 210
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 5 cut(s) 13, 89, 133, 212, 236
BtuMI TCGCGA 1 cut(s) 101
Cac8I GCNNGC 5 cut(s) 11, 56, 60, 91, 131
CfoI GCGC 2 cut(s) 54, 167
Cfr13I GGNCC 5 cut(s) 11, 12, 88, 131, 132
CviAII CATG 2 cut(s) 55, 59
DdeI CTNAG 2 cut(s) 317, 447
DpnI GATC 3 cut(s) 289, 304, 322
DpnII GATC 3 cut(s) 287, 302, 320
EaeI YGGCCR 2 cut(s) 210, 234
EagI CGGCCG 1 cut(s) 210
EclXI CGGCCG 1 cut(s) 210
Eco24I GRGCYC 2 cut(s) 15, 135
Eco31I GGTCTC 1 cut(s) 169
Eco52I CGGCCG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 60
EcoT38I GRGCYC 2 cut(s) 15, 135
FaeI CATG 2 cut(s) 58, 62
FaiI YATR 2 cut(s) 56, 60
FatI CATG 2 cut(s) 54, 58
FauI CCCGC 4 cut(s) 2, 98, 122, 246
Fnu4HI GCNGC 1 cut(s) 7
FriOI GRGCYC 2 cut(s) 15, 135
Fsp4HI GCNGC 1 cut(s) 7
FspBI CTAG 1 cut(s) 378
GlaI GCGC 2 cut(s) 53, 166
GluI GCNGC 1 cut(s) 7
HaeIII GGCC 5 cut(s) 13, 89, 133, 212, 236
HapII CCGG 3 cut(s) 24, 247, 353
HhaI GCGC 2 cut(s) 54, 167
Hin1II CATG 2 cut(s) 58, 62
Hin6I GCGC 2 cut(s) 52, 165
HinP1I GCGC 2 cut(s) 52, 165
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 2 cut(s) 415, 436
HpaII CCGG 3 cut(s) 24, 247, 353
Hpy166II GTNNAC 2 cut(s) 336, 385
Hpy188I TCNGA 3 cut(s) 156, 441, 448
Hpy188III TCNNGA 5 cut(s) 100, 183, 247, 291, 353
Hpy8I GTNNAC 2 cut(s) 336, 385
Hpy99I CGWCG 3 cut(s) 101, 184, 211
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 150, 180
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 68
HpyF3I CTNAG 2 cut(s) 317, 447
Hsp92II CATG 2 cut(s) 58, 62
HspAI GCGC 2 cut(s) 52, 165
Kpn2I TCCGGA 2 cut(s) 246, 352
Kzo9I GATC 3 cut(s) 287, 302, 320
LmnI GCTCC 1 cut(s) 156
LpnPI CCDG 5 cut(s) 37, 260, 344, 366, 446
LweI GCATC 1 cut(s) 110
MaeI CTAG 1 cut(s) 378
MalI GATC 3 cut(s) 289, 304, 322
MboI GATC 3 cut(s) 287, 302, 320
MboII GAAGA 2 cut(s) 279, 402
MfeI CAATTG 1 cut(s) 344
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 2 cut(s) 15, 135
MluCI AATT 3 cut(s) 195, 344, 406
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 134
MnlI CCTC 5 cut(s) 92, 104, 200, 243, 293
Mph1103I ATGCAT 1 cut(s) 60
MroI TCCGGA 2 cut(s) 246, 352
MseI TTAA 1 cut(s) 225
MspI CCGG 3 cut(s) 24, 247, 353
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 1 cut(s) 344
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 49, 68
NciI CCSGG 1 cut(s) 24
NdeII GATC 3 cut(s) 287, 302, 320
NlaIII CATG 2 cut(s) 58, 62
NlaIV GGNNCC 5 cut(s) 13, 133, 245, 304, 351
NruI TCGCGA 1 cut(s) 101
NsiI ATGCAT 1 cut(s) 60
NspI RCATGY 2 cut(s) 58, 62
PaeI GCATGC 2 cut(s) 58, 62
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 1 cut(s) 8
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
PspN4I GGNNCC 5 cut(s) 13, 133, 245, 304, 351
PspOMI GGGCCC 2 cut(s) 11, 131
PspPI GGNCC 5 cut(s) 11, 12, 88, 131, 132
PsuI RGATCY 1 cut(s) 302
RruI TCGCGA 1 cut(s) 101
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 3 cut(s) 287, 302, 320
Sau96I GGNCC 5 cut(s) 11, 12, 88, 131, 132
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 2 cut(s) 15, 135
SetI ASST 3 cut(s) 161, 231, 245
SfaNI GCATC 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 83, 160
SmoI CTYRAG 2 cut(s) 83, 160
SphI GCATGC 2 cut(s) 58, 62
Sse9I AATT 3 cut(s) 195, 344, 406
SsiI CCGC 6 cut(s) 6, 9, 43, 91, 129, 253
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 150, 180
TaqI TCGA 4 cut(s) 182, 290, 323, 413
TasI AATT 3 cut(s) 195, 344, 406
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TspGWI ACGGA 2 cut(s) 195, 353
XceI RCATGY 2 cut(s) 58, 62
XspI CTAG 1 cut(s) 378
Zsp2I ATGCAT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.