Rroxscaffold_6G00415450

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005832
Physical Location & Seq
Reverse (-)
37643431 .. 37645839
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00415450.1

Sequence Viewer

Length: 1035 bp
ATGTCAATCCTCCACACCTCTCTCACACACCAATCCCTCTCCTTGTCGAAATCCCTAACCTCCCCAACCCCTCTGTTCCTCCACACAAACCTCTCCCTTAAACCCTTAACCCCAAAGCCCACATTCATCCGAATGGGCGGCGGGCCCAGGACCTTCCCCGGCGGCGTATCCAAGTGGCAGTGGAAGCGCATGCAGGCCAAGAAAGCCAAGCAGCTCCTCAAGGCCCGCCTCTGCCGCGAGCGCCAAATCTACGAGATGCGAAAGCGGGCCGAGCTCAAGGCCGCCGTGTCGGAGCTCGAGCGCCCCTGGGAGACCGTCGAGAAGCCTCCCAATTTGTTCTCCGTCAGCGCCGATGAGCAGGTTAAGGTGTTGGCCGATCGGTTCCAGAGGCCGGGAGGGTTTGACCTGTGGACGGAGAGGGATGGGCCGGAGCTGTTTCGGACCATGGAGGACCTGCCGTCGGCGAGGTTTTTTCCGAAGGGGGTTGTGCATAGTGTGAGGCCGTATAGGAGGATTGGGGCTGAGGAATTGGGGGAGGAGGAGGAAGATGAGGGTTTTGGAGGAGTCAAGGGGTTGGTTGATGGGCTTAAGGGTGCAGAGAGGTCCAATGGGGAGGGTTTGAATTCGAAAAGTTCTTTGGAGTTGGTGAATGGTCGAAATGGGAGGAGGATGTATAATGGTAAGCTGAGGAGGAAGGGGAAAGGGAATAGGGGAAATGCTGAAGCTTCAAGTGATGTTGATAGGAAACAGAGAGGACAGAGTCCTGTAATGCGCAGTGGCGAAAATGGGAATTATAGGAGAAATGGTGGATTGAAATTGAACGGTTTGGAGGATGATGGTGTTGATAGGAAGTCTTCGAGAAGGTATAATTCGAGAGAAGGTGGTGTTGATAGGAGACAGAGAGGACAGGGTGGCATAATCCGCGGTGCGCAAAATACGCGTGATAGAAGAAAAGGTGCATTGAAATCGAAAGGTTCAGAGTCTGAAGTTTTCGATATGAGGTTGCGACAAGATGGGAGTTATGAATTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

38.75

Weight (kDa)

10.3

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 773, 930
Acc36I ACCTGC 2 cut(s) 349, 462
AccII CGCG 3 cut(s) 237, 924, 940
AciI CCGC 9 cut(s) 138, 141, 162, 226, 235, 265, 282, 922, 924
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 2 cut(s) 622, 1025
AcuI CTGAAG 2 cut(s) 741, 1005
AfiI CCNNNNNNNGG 3 cut(s) 391, 412, 460
AflII CTTAAG 1 cut(s) 587
AflIII ACRYGT 1 cut(s) 938
AgsI TTSAA 6 cut(s) 622, 729, 814, 820, 964, 1031
AhdI GACNNNNNGTC 1 cut(s) 457
AjnI CCWGG 2 cut(s) 146, 305
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AluBI AGCT 6 cut(s) 214, 274, 295, 433, 685, 725
AluI AGCT 6 cut(s) 214, 274, 295, 433, 685, 725
Alw21I GWGCWC 2 cut(s) 276, 297
Alw26I GTCTC 2 cut(s) 305, 889
AlwNI CAGNNNCTG 1 cut(s) 983
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 9 cut(s) 143, 195, 222, 267, 279, 372, 389, 425, 500
ApaI GGGCCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 211
ApoI RAATTY 2 cut(s) 622, 1025
AspLEI GCGC 6 cut(s) 189, 243, 303, 350, 774, 931
AspS9I GGNCC 9 cut(s) 143, 144, 150, 223, 267, 425, 441, 451, 603
AsuC2I CCSGG 2 cut(s) 159, 393
AsuHPI GGTGA 1 cut(s) 658
AsuII TTCGAA 1 cut(s) 626
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 4 cut(s) 150, 441, 451, 603
BaeGI GKGCMC 1 cut(s) 147
BanII GRGCYC 3 cut(s) 147, 276, 297
BbsI GAAGAC 1 cut(s) 846
Bbv12I GWGCWC 2 cut(s) 276, 297
BbvCI CCTCAGC 2 cut(s) 522, 686
BbvI GCAGC 1 cut(s) 223
BccI CCATC 4 cut(s) 416, 575, 830, 1007
BceAI ACGGC 3 cut(s) 269, 442, 487
BcgI CGANNNNNNTGC 2 cut(s) 948, 982
BciT130I CCWGG 2 cut(s) 148, 307
BciVI GTATCC 1 cut(s) 178
BcnI CCSGG 2 cut(s) 159, 393
BcoDI GTCTC 2 cut(s) 305, 889
BfoI RGCGCY 3 cut(s) 244, 304, 351
BfrI CTTAAG 1 cut(s) 587
BfuAI ACCTGC 2 cut(s) 349, 462
BfuI GTATCC 1 cut(s) 178
BisI GCNGC 5 cut(s) 139, 163, 212, 235, 282
BlsI GCNGC 5 cut(s) 140, 164, 213, 236, 283
Bme1390I CCNGG 4 cut(s) 148, 159, 307, 393
Bme18I GGWCC 4 cut(s) 150, 441, 451, 603
BmeRI GACNNNNNGTC 1 cut(s) 457
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 9 cut(s) 143, 144, 150, 223, 267, 425, 441, 451, 603
BmiI GGNNCC 2 cut(s) 145, 383
BmrFI CCNGG 4 cut(s) 148, 159, 307, 393
BmsI GCATC 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 846
Bpu10I CCTNAGC 2 cut(s) 522, 686
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 203, 260
BpuMI CCSGG 2 cut(s) 159, 393
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 6 cut(s) 146, 157, 305, 306, 444, 922
BsaXI ACNNNNNCTCC 2 cut(s) 790, 820
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 412, 460
BseBI CCWGG 2 cut(s) 148, 307
BseDI CCNNGG 6 cut(s) 146, 157, 305, 306, 444, 922
BseGI GGATG 4 cut(s) 126, 427, 675, 838
BseLI CCNNNNNNNGG 3 cut(s) 391, 412, 460
BseMII CTCAG 2 cut(s) 513, 677
BseRI GAGGAG 6 cut(s) 206, 551, 554, 576, 679, 703
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 3 cut(s) 237, 924, 940
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 9 cut(s) 145, 197, 224, 269, 281, 374, 391, 427, 502
BsiEI CGRYCG 1 cut(s) 379
BsiHKAI GWGCWC 2 cut(s) 276, 297
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 3 cut(s) 159, 392, 428
BslI CCNNNNNNNGG 3 cut(s) 391, 412, 460
BsmAI GTCTC 2 cut(s) 305, 889
BsnI GGCC 9 cut(s) 145, 197, 224, 269, 281, 374, 391, 427, 502
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 626
Bsp120I GGGCCC 1 cut(s) 143
Bsp1286I GDGCHC 3 cut(s) 147, 276, 297
Bsp143I GATC 1 cut(s) 376
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 9 cut(s) 138, 141, 162, 226, 235, 265, 282, 922, 924
BspANI GGCC 9 cut(s) 145, 197, 224, 269, 281, 374, 391, 427, 502
BspCNI CTCAG 2 cut(s) 514, 678
BspFNI CGCG 3 cut(s) 237, 924, 940
BspLI GGNNCC 2 cut(s) 145, 383
BspMI ACCTGC 2 cut(s) 349, 462
BspT104I TTCGAA 1 cut(s) 626
BspTI CTTAAG 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 305
BssECI CCNNGG 6 cut(s) 146, 157, 305, 306, 444, 922
BssMI GATC 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 444
Bst2UI CCWGG 2 cut(s) 148, 307
Bst4CI ACNGT 2 cut(s) 316, 824
BstAFI CTTAAG 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 6 cut(s) 143, 191, 195, 226, 239, 267
BstDEI CTNAG 2 cut(s) 522, 686
BstDSI CCRYGG 2 cut(s) 444, 922
BstF5I GGATG 4 cut(s) 126, 427, 675, 838
BstFNI CGCG 3 cut(s) 237, 924, 940
BstH2I RGCGCY 3 cut(s) 244, 304, 351
BstHHI GCGC 6 cut(s) 189, 243, 303, 350, 774, 931
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 2 cut(s) 305, 889
BstMBI GATC 1 cut(s) 376
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 7 cut(s) 184, 203, 234, 240, 271, 921, 937
BstNI CCWGG 2 cut(s) 148, 307
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 4 cut(s) 146, 157, 305, 391
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 3 cut(s) 237, 924, 940
BstV1I GCAGC 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 846
BsuI GTATCC 1 cut(s) 178
BsuRI GGCC 9 cut(s) 145, 197, 224, 269, 281, 374, 391, 427, 502
BtgI CCRYGG 2 cut(s) 444, 922
BtsCI GGATG 4 cut(s) 126, 427, 675, 838
BtsI GCAGTG 2 cut(s) 185, 781
BtsIMutI CAGTG 2 cut(s) 185, 781
BveI ACCTGC 2 cut(s) 349, 462
Cac8I GCNNGC 6 cut(s) 143, 191, 195, 226, 239, 267
CaiI CAGNNNCTG 1 cut(s) 983
CfoI GCGC 6 cut(s) 189, 243, 303, 350, 774, 931
Cfr13I GGNCC 9 cut(s) 143, 144, 150, 223, 267, 425, 441, 451, 603
Cfr42I CCGCGG 1 cut(s) 925
CviAII CATG 2 cut(s) 190, 445
DdeI CTNAG 2 cut(s) 522, 686
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
DriI GACNNNNNGTC 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 372
Eam1105I GACNNNNNGTC 1 cut(s) 457
Ecl136II GAGCTC 2 cut(s) 274, 295
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 3 cut(s) 147, 276, 297
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 4 cut(s) 150, 441, 451, 603
Eco53kI GAGCTC 2 cut(s) 274, 295
Eco57I CTGAAG 2 cut(s) 741, 1005
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 2 cut(s) 274, 295
EcoO109I RGGNCCY 2 cut(s) 150, 451
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 2 cut(s) 146, 305
EcoT14I CCWWGG 1 cut(s) 444
EcoT38I GRGCYC 3 cut(s) 147, 276, 297
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 2 cut(s) 193, 448
FatI CATG 2 cut(s) 189, 444
FauI CCCGC 3 cut(s) 134, 233, 258
Fnu4HI GCNGC 5 cut(s) 139, 163, 212, 235, 282
FokI GGATG 4 cut(s) 113, 434, 682, 845
FriOI GRGCYC 3 cut(s) 147, 276, 297
Fsp4HI GCNGC 5 cut(s) 139, 163, 212, 235, 282
FspI TGCGCA 2 cut(s) 773, 930
GlaI GCGC 6 cut(s) 188, 242, 302, 349, 773, 930
GluI GCNGC 5 cut(s) 139, 163, 212, 235, 282
HaeII RGCGCY 3 cut(s) 244, 304, 351
HaeIII GGCC 9 cut(s) 145, 197, 224, 269, 281, 374, 391, 427, 502
HapII CCGG 3 cut(s) 159, 392, 428
HhaI GCGC 6 cut(s) 189, 243, 303, 350, 774, 931
Hin1II CATG 2 cut(s) 193, 448
Hin6I GCGC 6 cut(s) 187, 241, 301, 348, 772, 929
HinP1I GCGC 6 cut(s) 187, 241, 301, 348, 772, 929
HindIII AAGCTT 1 cut(s) 723
HinfI GANTC 3 cut(s) 564, 760, 980
HpaII CCGG 3 cut(s) 159, 392, 428
HphI GGTGA 1 cut(s) 658
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 6 cut(s) 131, 292, 441, 477, 979, 985
Hpy188III TCNNGA 4 cut(s) 319, 385, 858, 873
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 2 cut(s) 320, 463
HpyAV CCTTC 5 cut(s) 163, 472, 688, 855, 872
HpyCH4III ACNGT 2 cut(s) 316, 824
HpyCH4V TGCA 4 cut(s) 193, 490, 596, 959
HpyF10VI GCNNNNNNNGC 7 cut(s) 184, 203, 234, 240, 271, 921, 937
HpyF3I CTNAG 2 cut(s) 522, 686
Hsp92II CATG 2 cut(s) 193, 448
HspAI GCGC 6 cut(s) 187, 241, 301, 348, 772, 929
KspI CCGCGG 1 cut(s) 925
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 3 cut(s) 219, 292, 430
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 246
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 3 cut(s) 557, 846, 960
MhlI GDGCHC 3 cut(s) 147, 276, 297
MluCI AATT 7 cut(s) 331, 527, 622, 790, 815, 868, 1025
MluI ACGCGT 1 cut(s) 938
MlyI GAGTC 3 cut(s) 573, 769, 989
MmeI TCCRAC 1 cut(s) 270
MseI TTAA 4 cut(s) 99, 107, 363, 588
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 924
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 3 cut(s) 159, 392, 428
MspR9I CCNGG 4 cut(s) 148, 159, 307, 393
MvaI CCWGG 2 cut(s) 148, 307
MvnI CGCG 3 cut(s) 237, 924, 940
MwoI GCNNNNNNNGC 7 cut(s) 184, 203, 234, 240, 271, 921, 937
NciI CCSGG 2 cut(s) 159, 393
NcoI CCATGG 1 cut(s) 444
NdeII GATC 1 cut(s) 376
NlaIII CATG 2 cut(s) 193, 448
NlaIV GGNNCC 2 cut(s) 145, 383
NmeAIII GCCGAG 1 cut(s) 295
NsbI TGCGCA 2 cut(s) 773, 930
NspI RCATGY 1 cut(s) 193
NspV TTCGAA 1 cut(s) 626
PaeI GCATGC 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 296
PasI CCCWGGG 1 cut(s) 306
PflFI GACNNNGTC 1 cut(s) 759
PkrI GCNGC 5 cut(s) 140, 164, 213, 236, 283
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 3 cut(s) 572, 768, 988
PpsI GAGTC 3 cut(s) 572, 768, 988
PpuMI RGGWCCY 2 cut(s) 150, 451
Psp124BI GAGCTC 2 cut(s) 276, 297
Psp5II RGGWCCY 2 cut(s) 150, 451
Psp6I CCWGG 2 cut(s) 146, 305
PspGI CCWGG 2 cut(s) 146, 305
PspN4I GGNNCC 2 cut(s) 145, 383
PspOMI GGGCCC 1 cut(s) 143
PspPI GGNCC 9 cut(s) 143, 144, 150, 223, 267, 425, 441, 451, 603
PspPPI RGGWCCY 2 cut(s) 150, 451
PspXI VCTCGAGB 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 983
PsyI GACNNNGTC 1 cut(s) 759
PvuI CGATCG 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 2 cut(s) 276, 297
SacII CCGCGG 1 cut(s) 925
SaqAI TTAA 4 cut(s) 99, 107, 363, 588
SatI GCNGC 5 cut(s) 139, 163, 212, 235, 282
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 9 cut(s) 143, 144, 150, 223, 267, 425, 441, 451, 603
SchI GAGTC 3 cut(s) 573, 769, 989
ScrFI CCNGG 4 cut(s) 148, 159, 307, 393
SduI GDGCHC 3 cut(s) 147, 276, 297
SfaNI GCATC 1 cut(s) 246
Sfr274I CTCGAG 1 cut(s) 296
Sfr303I CCGCGG 1 cut(s) 925
SfuI TTCGAA 1 cut(s) 626
SgrBI CCGCGG 1 cut(s) 925
SinI GGWCC 4 cut(s) 150, 441, 451, 603
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 4 cut(s) 218, 275, 296, 587
SmoI CTYRAG 4 cut(s) 218, 275, 296, 587
SphI GCATGC 1 cut(s) 193
Sse9I AATT 7 cut(s) 331, 527, 622, 790, 815, 868, 1025
SsiI CCGC 9 cut(s) 138, 141, 162, 226, 235, 265, 282, 922, 924
SstI GAGCTC 2 cut(s) 276, 297
StyD4I CCNGG 4 cut(s) 146, 157, 305, 391
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 2 cut(s) 316, 824
TaqI TCGA 9 cut(s) 47, 297, 318, 626, 655, 857, 872, 968, 993
TasI AATT 7 cut(s) 331, 527, 622, 790, 815, 868, 1025
TauI GCSGC 4 cut(s) 141, 165, 237, 284
Tru1I TTAA 4 cut(s) 99, 107, 363, 588
Tru9I TTAA 4 cut(s) 99, 107, 363, 588
TscAI CASTG 2 cut(s) 185, 781
TseI GCWGC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 115
TspGWI ACGGA 2 cut(s) 331, 428
TspRI CASTG 2 cut(s) 185, 781
Tth111I GACNNNGTC 1 cut(s) 759
Vha464I CTTAAG 1 cut(s) 587
VpaK11BI GGWCC 4 cut(s) 150, 441, 451, 603
XapI RAATTY 2 cut(s) 622, 1025
XceI RCATGY 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.