RLG00000024675

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005832
Physical Location & Seq
Reverse (-)
38103303 .. 38104328
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024675

Sequence Viewer

Length: 1026 bp
ATGTCAATCCTCCACACCTCTCTCACACACCAATCCCTCTCCTTCTCCAAATCCCTAACGTCCCCAACCCCTCTCTTCCTCCACAGGTCAAACCTCTCCCTTAAACCGCTAAGCCCAAAGCCCACATTCATCCGAATGGGCGGCGGGCCCAGAACCTTCCCCGGCGGCGTATCCAAGTGGCAGTGGAAGCGCATGCAGGCGAAGAAAGCCAAGCAGCTCCTCAAGGCCCGCCTCTGCCGCGAGCGCCAAATCTACGAGATGCGAAAGCGGGCCGAGCTCAAGGCCGCCGTGTCGGAGCTCGAGCGCCCCTGGGAGACTGTCGAGAAGCCTCCCAATTTGTTCTCCGTCAGCGCCGACGAGCAGGTTAAGGTGTTGGCCGATAGGTTCCAGAGGCCGGGAGGGTTTGACCTGTGGACCGAGAGGGATGGGCCGGAGCTGTTTCGCACCATGGAGGACCCGCCGTCGGCGAGGTTTTTTCCCAAGGGGGTTGTGCATAGTGTGAGGCCGTATAGGAGGATTGGGGCTGAGGAATTGGGGGAGGAGGAAGATGAGGGTTTTGGAGGAGTCAAGGGGTTGGTTGATGGGCCTAAGGGTGCAGAGAGGTCAAATGGGGAGGGTTTGAATTCGAAAAGTTCTTCGGAGTCGGTGAATTTTCGAAATGGGAGGATGTATAATGGTAAACTGAGGAGGAAGGGGAACAGGGGAAATGCGGAAGCTTCAAGTGATGTTGATAGGAAACAGAGAGGACAGAGTCCTGTAATGCCAAGTGGCGAAAATGGGAATTCTAGGAGAAATGGTGGATTGAAGTTGAATGGTTTGGAGGATGATGGTGTTGATAGGAGGTCTTCGAGTGGGTATAATTCGAGAGAAGGTGGTGTCCATAGGAGACAGAGAGGACAGGGTCGCATAATCCGCAGTGGGGAAAATACGCGTGATAGAAGAAAAGGTGCATTGAAATCGAAAGGTTCAGAGTCTGAAGTTTTCGATATGAGGTTGCGACAAGATGGGTGTTATGAATTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

38.32

Weight (kDa)

10.24

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 352
AccII CGCG 2 cut(s) 240, 931
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 4 cut(s) 622, 649, 781, 1016
AcuI CTGAAG 1 cut(s) 996
AfiI CCNNNNNNNGG 3 cut(s) 394, 463, 919
AflIII ACRYGT 1 cut(s) 929
AgsI TTSAA 6 cut(s) 622, 720, 805, 811, 955, 1022
AhdI GACNNNNNGTC 1 cut(s) 460
AjnI CCWGG 1 cut(s) 308
AluBI AGCT 5 cut(s) 217, 277, 298, 436, 716
AluI AGCT 5 cut(s) 217, 277, 298, 436, 716
Alw21I GWGCWC 2 cut(s) 279, 300
Alw26I GTCTC 2 cut(s) 308, 880
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 9 cut(s) 146, 225, 270, 282, 375, 392, 428, 503, 584
ApaI GGGCCC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 214
ApoI RAATTY 4 cut(s) 622, 649, 781, 1016
AspLEI GCGC 4 cut(s) 192, 246, 306, 353
AspS9I GGNCC 8 cut(s) 146, 147, 226, 270, 414, 428, 454, 584
AsuC2I CCSGG 2 cut(s) 162, 396
AsuHPI GGTGA 1 cut(s) 658
AsuII TTCGAA 2 cut(s) 626, 655
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 2 cut(s) 414, 454
AxyI CCTNAGG 1 cut(s) 588
BaeGI GKGCMC 1 cut(s) 150
BanII GRGCYC 3 cut(s) 150, 279, 300
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 2 cut(s) 279, 300
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 226
BccI CCATC 4 cut(s) 419, 575, 821, 998
BceAI ACGGC 3 cut(s) 272, 445, 490
BcgI CGANNNNNNTGC 2 cut(s) 939, 973
BciT130I CCWGG 1 cut(s) 310
BciVI GTATCC 1 cut(s) 181
BcnI CCSGG 2 cut(s) 162, 396
BcoDI GTCTC 2 cut(s) 308, 880
BfaI CTAG 1 cut(s) 786
BfoI RGCGCY 3 cut(s) 247, 307, 354
BfuAI ACCTGC 1 cut(s) 352
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 5 cut(s) 142, 166, 215, 238, 285
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 5 cut(s) 143, 167, 216, 239, 286
Bme1390I CCNGG 3 cut(s) 162, 310, 396
Bme18I GGWCC 2 cut(s) 414, 454
BmeRI GACNNNNNGTC 1 cut(s) 460
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 8 cut(s) 146, 147, 226, 270, 414, 428, 454, 584
BmiI GGNNCC 3 cut(s) 148, 386, 456
BmrFI CCNGG 3 cut(s) 162, 310, 396
BmsI GCATC 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 837
Bpu10I CCTNAGC 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 110
Bpu14I TTCGAA 2 cut(s) 626, 655
BpuEI CTTGAG 2 cut(s) 206, 263
BpuMI CCSGG 2 cut(s) 162, 396
BsaJI CCNNGG 5 cut(s) 160, 308, 309, 447, 480
BsaXI ACNNNNNCTCC 2 cut(s) 781, 811
Bsc4I CCNNNNNNNGG 3 cut(s) 394, 463, 919
Bse21I CCTNAGG 1 cut(s) 588
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 5 cut(s) 160, 308, 309, 447, 480
BseGI GGATG 4 cut(s) 129, 430, 672, 829
BseLI CCNNNNNNNGG 3 cut(s) 394, 463, 919
BseMII CTCAG 2 cut(s) 516, 674
BseRI GAGGAG 4 cut(s) 209, 554, 576, 700
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 2 cut(s) 240, 931
BshFI GGCC 9 cut(s) 148, 227, 272, 284, 377, 394, 430, 505, 586
BsiHKAI GWGCWC 2 cut(s) 279, 300
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 162, 395, 431
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 3 cut(s) 394, 463, 919
BsmAI GTCTC 2 cut(s) 308, 880
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 9 cut(s) 148, 227, 272, 284, 377, 394, 430, 505, 586
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 2 cut(s) 626, 655
Bsp120I GGGCCC 1 cut(s) 146
Bsp1286I GDGCHC 3 cut(s) 150, 279, 300
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 1 cut(s) 447
BspANI GGCC 9 cut(s) 148, 227, 272, 284, 377, 394, 430, 505, 586
BspCNI CTCAG 2 cut(s) 517, 675
BspFNI CGCG 2 cut(s) 240, 931
BspLI GGNNCC 3 cut(s) 148, 386, 456
BspMI ACCTGC 1 cut(s) 352
BspT104I TTCGAA 2 cut(s) 626, 655
BssECI CCNNGG 5 cut(s) 160, 308, 309, 447, 480
BssT1I CCWWGG 2 cut(s) 447, 480
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 80
BstBI TTCGAA 2 cut(s) 626, 655
BstC8I GCNNGC 6 cut(s) 146, 194, 198, 229, 242, 270
BstDEI CTNAG 4 cut(s) 110, 525, 588, 683
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 4 cut(s) 129, 430, 672, 829
BstFNI CGCG 2 cut(s) 240, 931
BstH2I RGCGCY 3 cut(s) 247, 307, 354
BstHHI GCGC 4 cut(s) 192, 246, 306, 353
BstMAI GTCTC 2 cut(s) 308, 880
BstMWI GCNNNNNNNGC 6 cut(s) 187, 206, 237, 243, 274, 912
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 160, 308, 394
BstSLI GKGCMC 1 cut(s) 150
BstUI CGCG 2 cut(s) 240, 931
BstV1I GCAGC 1 cut(s) 226
BstV2I GAAGAC 1 cut(s) 837
Bsu36I CCTNAGG 1 cut(s) 588
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 9 cut(s) 148, 227, 272, 284, 377, 394, 430, 505, 586
BtgI CCRYGG 1 cut(s) 447
BtsCI GGATG 4 cut(s) 129, 430, 672, 829
BtsI GCAGTG 2 cut(s) 188, 922
BtsIMutI CAGTG 2 cut(s) 188, 922
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 6 cut(s) 146, 194, 198, 229, 242, 270
CaiI CAGNNNCTG 1 cut(s) 974
CfoI GCGC 4 cut(s) 192, 246, 306, 353
Cfr13I GGNCC 8 cut(s) 146, 147, 226, 270, 414, 428, 454, 584
CviAII CATG 2 cut(s) 193, 448
DdeI CTNAG 4 cut(s) 110, 525, 588, 683
DriI GACNNNNNGTC 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 80
Eam1105I GACNNNNNGTC 1 cut(s) 460
EarI CTCTTC 1 cut(s) 80
Ecl136II GAGCTC 2 cut(s) 277, 298
Eco130I CCWWGG 2 cut(s) 447, 480
Eco24I GRGCYC 3 cut(s) 150, 279, 300
Eco47I GGWCC 2 cut(s) 414, 454
Eco53kI GAGCTC 2 cut(s) 277, 298
Eco57I CTGAAG 1 cut(s) 996
Eco81I CCTNAGG 1 cut(s) 588
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 2 cut(s) 277, 298
EcoO109I RGGNCCY 1 cut(s) 454
EcoRI GAATTC 2 cut(s) 622, 781
EcoRII CCWGG 1 cut(s) 308
EcoT14I CCWWGG 2 cut(s) 447, 480
EcoT38I GRGCYC 3 cut(s) 150, 279, 300
ErhI CCWWGG 2 cut(s) 447, 480
FaeI CATG 2 cut(s) 196, 451
FaqI GGGAC 1 cut(s) 46
FatI CATG 2 cut(s) 192, 447
FauI CCCGC 4 cut(s) 137, 236, 261, 465
Fnu4HI GCNGC 5 cut(s) 142, 166, 215, 238, 285
FokI GGATG 4 cut(s) 116, 437, 679, 836
FriOI GRGCYC 3 cut(s) 150, 279, 300
Fsp4HI GCNGC 5 cut(s) 142, 166, 215, 238, 285
FspBI CTAG 1 cut(s) 786
GlaI GCGC 4 cut(s) 191, 245, 305, 352
GluI GCNGC 5 cut(s) 142, 166, 215, 238, 285
HaeII RGCGCY 3 cut(s) 247, 307, 354
HaeIII GGCC 9 cut(s) 148, 227, 272, 284, 377, 394, 430, 505, 586
HapII CCGG 3 cut(s) 162, 395, 431
HhaI GCGC 4 cut(s) 192, 246, 306, 353
Hin1II CATG 2 cut(s) 196, 451
Hin6I GCGC 4 cut(s) 190, 244, 304, 351
HinP1I GCGC 4 cut(s) 190, 244, 304, 351
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 4 cut(s) 564, 641, 751, 971
HpaII CCGG 3 cut(s) 162, 395, 431
HphI GGTGA 1 cut(s) 658
Hpy166II GTNNAC 2 cut(s) 414, 680
Hpy188I TCNGA 5 cut(s) 134, 295, 640, 970, 976
Hpy188III TCNNGA 3 cut(s) 322, 388, 864
Hpy8I GTNNAC 2 cut(s) 414, 680
Hpy99I CGWCG 2 cut(s) 359, 466
HpyAV CCTTC 4 cut(s) 52, 166, 685, 863
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 4 cut(s) 196, 493, 596, 950
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 206, 237, 243, 274, 912
HpyF3I CTNAG 4 cut(s) 110, 525, 588, 683
HpySE526I ACGT 1 cut(s) 59
Hsp92II CATG 2 cut(s) 196, 451
HspAI GCGC 4 cut(s) 190, 244, 304, 351
LmnI GCTCC 3 cut(s) 222, 295, 433
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 786
MaeII ACGT 1 cut(s) 59
MboII GAAGA 6 cut(s) 67, 214, 557, 627, 837, 951
MhlI GDGCHC 3 cut(s) 150, 279, 300
MluCI AATT 7 cut(s) 334, 530, 622, 649, 781, 859, 1016
MluI ACGCGT 1 cut(s) 929
MlyI GAGTC 4 cut(s) 573, 650, 760, 980
MmeI TCCRAC 1 cut(s) 273
MseI TTAA 2 cut(s) 102, 366
MslI CAYNNNNRTG 1 cut(s) 134
MspI CCGG 3 cut(s) 162, 395, 431
MspR9I CCNGG 3 cut(s) 162, 310, 396
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 2 cut(s) 240, 931
MwoI GCNNNNNNNGC 6 cut(s) 187, 206, 237, 243, 274, 912
NciI CCSGG 2 cut(s) 162, 396
NcoI CCATGG 1 cut(s) 447
NlaIII CATG 2 cut(s) 196, 451
NlaIV GGNNCC 3 cut(s) 148, 386, 456
NmeAIII GCCGAG 1 cut(s) 298
NspI RCATGY 1 cut(s) 196
NspV TTCGAA 2 cut(s) 626, 655
PaeI GCATGC 1 cut(s) 196
PaeR7I CTCGAG 1 cut(s) 299
PasI CCCWGGG 1 cut(s) 309
PflFI GACNNNGTC 2 cut(s) 750, 900
PkrI GCNGC 5 cut(s) 143, 167, 216, 239, 286
PleI GAGTC 4 cut(s) 572, 649, 759, 979
PpsI GAGTC 4 cut(s) 572, 649, 759, 979
PpuMI RGGWCCY 1 cut(s) 454
Psp124BI GAGCTC 2 cut(s) 279, 300
Psp5II RGGWCCY 1 cut(s) 454
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 3 cut(s) 148, 386, 456
PspOMI GGGCCC 1 cut(s) 146
PspPI GGNCC 8 cut(s) 146, 147, 226, 270, 414, 428, 454, 584
PspPPI RGGWCCY 1 cut(s) 454
PspXI VCTCGAGB 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 974
PsyI GACNNNGTC 2 cut(s) 750, 900
RseI CAYNNNNRTG 1 cut(s) 134
SacI GAGCTC 2 cut(s) 279, 300
SaqAI TTAA 2 cut(s) 102, 366
SatI GCNGC 5 cut(s) 142, 166, 215, 238, 285
Sau96I GGNCC 8 cut(s) 146, 147, 226, 270, 414, 428, 454, 584
SchI GAGTC 4 cut(s) 573, 650, 760, 980
ScrFI CCNGG 3 cut(s) 162, 310, 396
SduI GDGCHC 3 cut(s) 150, 279, 300
SfaNI GCATC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 299
SfuI TTCGAA 2 cut(s) 626, 655
SinI GGWCC 2 cut(s) 414, 454
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 3 cut(s) 221, 278, 299
SmoI CTYRAG 3 cut(s) 221, 278, 299
SphI GCATGC 1 cut(s) 196
Sse9I AATT 7 cut(s) 334, 530, 622, 649, 781, 859, 1016
SspMI CTAG 1 cut(s) 786
SstI GAGCTC 2 cut(s) 279, 300
StyD4I CCNGG 3 cut(s) 160, 308, 394
StyI CCWWGG 2 cut(s) 447, 480
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 62
TaqI TCGA 8 cut(s) 300, 321, 626, 655, 848, 863, 959, 984
TaqII GACCGA 1 cut(s) 431
TasI AATT 7 cut(s) 334, 530, 622, 649, 781, 859, 1016
TauI GCSGC 4 cut(s) 144, 168, 240, 287
Tru1I TTAA 2 cut(s) 102, 366
Tru9I TTAA 2 cut(s) 102, 366
TscAI CASTG 2 cut(s) 188, 922
TseI GCWGC 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 118
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 2 cut(s) 188, 922
Tth111I GACNNNGTC 2 cut(s) 750, 900
VpaK11BI GGWCC 2 cut(s) 414, 454
XapI RAATTY 4 cut(s) 622, 649, 781, 1016
XceI RCATGY 1 cut(s) 196
XhoI CTCGAG 1 cut(s) 299
XspI CTAG 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.