RchiOBHm_Chr3g0464731

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005832
Physical Location & Seq
Forward (+)
11736622 .. 11738117
1496 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43094

Sequence Viewer

Length: 618 bp
ATGTCAATCCTCCACACCTCTCTCACACACCAATCCCTCTCCTTCTCCAAATCCCTGACCTCCCCAACCCCTCTCTTCCTCCACACAAACCTCTCCCTTAAACCCTTAACCCCAAAGCCCACATTCATCCGAATGGGCGGCGGGCCCAGGACCTTCCCCGGCGGCGTATCCAAGTGGCAGTGGAAGCGCATGCAGGCCAAGAAAGCCAAGCAGCTCCTCAAGGCCCGCCTCTGCCGCGAGCGCCAAATCTACGAGATGCGAAAGCGGGCGGAGCTCAAGGCCGCCGTGTCGGAGCTCGAGCGCCCCTGGGAGACCGTCGAGAAGCCTCCCAATTTGTTCTCCGTCAGCGCCGATGAGCAGGTGAAGGTCTTGGCCGATCGGTTCCAGAGGCCGGGAGGGTTTGACCTGTGGACCGAGAGGGATGGGCCGGAGCTGTTCCGGACCATGGAGGACCTGCCGTCGGCAAGGTTTTTTCCCAAGGGGGTAGTGCATAGTGTGAGGCCGTACAGGAGGATTGGGGCTGAGGAATTGGGGGAGGAGGAAGATGAGGGTTTTGGAGGAGTCAAGGGATTGGTTGAGGGCTTAAGGGTGCAGAGAGGTCAAATGGGGAGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

23.35

Weight (kDa)

10.04

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 349
Acc36I ACCTGC 2 cut(s) 349, 462
AccII CGCG 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 438
AciI CCGC 8 cut(s) 138, 141, 162, 226, 235, 265, 269, 282
AcoI YGGCCR 1 cut(s) 372
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 3 cut(s) 391, 445, 460
AflII CTTAAG 1 cut(s) 583
AhdI GACNNNNNGTC 1 cut(s) 457
AjnI CCWGG 2 cut(s) 146, 305
AluBI AGCT 4 cut(s) 214, 274, 295, 433
AluI AGCT 4 cut(s) 214, 274, 295, 433
Alw21I GWGCWC 2 cut(s) 276, 297
Alw26I GTCTC 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 438
AoxI GGCC 8 cut(s) 143, 195, 222, 279, 372, 389, 425, 500
ApaI GGGCCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 211
AspLEI GCGC 4 cut(s) 189, 243, 303, 350
AspS9I GGNCC 8 cut(s) 143, 144, 150, 223, 411, 425, 441, 451
AsuC2I CCSGG 2 cut(s) 159, 393
AsuHPI GGTGA 1 cut(s) 373
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 4 cut(s) 150, 411, 441, 451
BaeGI GKGCMC 1 cut(s) 147
BanII GRGCYC 3 cut(s) 147, 276, 297
Bbv12I GWGCWC 2 cut(s) 276, 297
BbvCI CCTCAGC 1 cut(s) 522
BbvI GCAGC 1 cut(s) 223
BccI CCATC 1 cut(s) 416
BceAI ACGGC 3 cut(s) 269, 442, 487
BciT130I CCWGG 2 cut(s) 148, 307
BciVI GTATCC 1 cut(s) 178
BcnI CCSGG 2 cut(s) 159, 393
BcoDI GTCTC 1 cut(s) 305
BfoI RGCGCY 3 cut(s) 244, 304, 351
BfrI CTTAAG 1 cut(s) 583
BfuAI ACCTGC 2 cut(s) 349, 462
BfuI GTATCC 1 cut(s) 178
BisI GCNGC 5 cut(s) 139, 163, 212, 235, 282
BlsI GCNGC 5 cut(s) 140, 164, 213, 236, 283
Bme1390I CCNGG 4 cut(s) 148, 159, 307, 393
Bme18I GGWCC 4 cut(s) 150, 411, 441, 451
BmeRI GACNNNNNGTC 1 cut(s) 457
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 8 cut(s) 143, 144, 150, 223, 411, 425, 441, 451
BmiI GGNNCC 2 cut(s) 145, 383
BmrFI CCNGG 4 cut(s) 148, 159, 307, 393
BmsI GCATC 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 203, 260
BpuMI CCSGG 2 cut(s) 159, 393
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 6 cut(s) 146, 157, 305, 306, 444, 477
BsaWI WCCGGW 1 cut(s) 438
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 445, 460
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 2 cut(s) 148, 307
BseDI CCNNGG 6 cut(s) 146, 157, 305, 306, 444, 477
BseGI GGATG 2 cut(s) 126, 427
BseLI CCNNNNNNNGG 3 cut(s) 391, 445, 460
BseMII CTCAG 1 cut(s) 513
BseRI GAGGAG 3 cut(s) 206, 551, 573
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 237
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 8 cut(s) 145, 197, 224, 281, 374, 391, 427, 502
BsiEI CGRYCG 1 cut(s) 379
BsiHKAI GWGCWC 2 cut(s) 276, 297
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 4 cut(s) 159, 392, 428, 439
BslI CCNNNNNNNGG 3 cut(s) 391, 445, 460
BsmAI GTCTC 1 cut(s) 305
BsnI GGCC 8 cut(s) 145, 197, 224, 281, 374, 391, 427, 502
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 296
Bsp120I GGGCCC 1 cut(s) 143
Bsp1286I GDGCHC 3 cut(s) 147, 276, 297
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 1 cut(s) 376
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 8 cut(s) 138, 141, 162, 226, 235, 265, 269, 282
BspANI GGCC 8 cut(s) 145, 197, 224, 281, 374, 391, 427, 502
BspCNI CTCAG 1 cut(s) 514
BspEI TCCGGA 1 cut(s) 438
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 145, 383
BspMI ACCTGC 2 cut(s) 349, 462
BspTI CTTAAG 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 305
BssECI CCNNGG 6 cut(s) 146, 157, 305, 306, 444, 477
BssMI GATC 1 cut(s) 376
BssT1I CCWWGG 2 cut(s) 444, 477
Bst2UI CCWGG 2 cut(s) 148, 307
Bst4CI ACNGT 1 cut(s) 316
Bst6I CTCTTC 1 cut(s) 80
BstAFI CTTAAG 1 cut(s) 583
BstC8I GCNNGC 6 cut(s) 143, 191, 195, 226, 239, 267
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 1 cut(s) 444
BstF5I GGATG 2 cut(s) 126, 427
BstFNI CGCG 1 cut(s) 237
BstH2I RGCGCY 3 cut(s) 244, 304, 351
BstHHI GCGC 4 cut(s) 189, 243, 303, 350
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 1 cut(s) 376
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 5 cut(s) 184, 203, 234, 240, 271
BstNI CCWGG 2 cut(s) 148, 307
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 4 cut(s) 146, 157, 305, 391
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 223
BsuI GTATCC 1 cut(s) 178
BsuRI GGCC 8 cut(s) 145, 197, 224, 281, 374, 391, 427, 502
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 2 cut(s) 126, 427
BtsI GCAGTG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 185
BveI ACCTGC 2 cut(s) 349, 462
Cac8I GCNNGC 6 cut(s) 143, 191, 195, 226, 239, 267
CfoI GCGC 4 cut(s) 189, 243, 303, 350
Cfr13I GGNCC 8 cut(s) 143, 144, 150, 223, 411, 425, 441, 451
Csp6I GTAC 1 cut(s) 505
CviAII CATG 2 cut(s) 190, 445
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 1 cut(s) 522
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
DriI GACNNNNNGTC 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 80
Eam1105I GACNNNNNGTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 2 cut(s) 274, 295
Eco130I CCWWGG 2 cut(s) 444, 477
Eco24I GRGCYC 3 cut(s) 147, 276, 297
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 4 cut(s) 150, 411, 441, 451
Eco53kI GAGCTC 2 cut(s) 274, 295
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 2 cut(s) 274, 295
EcoO109I RGGNCCY 2 cut(s) 150, 451
EcoRII CCWGG 2 cut(s) 146, 305
EcoT14I CCWWGG 2 cut(s) 444, 477
EcoT38I GRGCYC 3 cut(s) 147, 276, 297
ErhI CCWWGG 2 cut(s) 444, 477
FaeI CATG 2 cut(s) 193, 448
FaiI YATR 3 cut(s) 191, 446, 492
FatI CATG 2 cut(s) 189, 444
FauI CCCGC 3 cut(s) 134, 233, 258
Fnu4HI GCNGC 5 cut(s) 139, 163, 212, 235, 282
FokI GGATG 2 cut(s) 113, 434
FriOI GRGCYC 3 cut(s) 147, 276, 297
Fsp4HI GCNGC 5 cut(s) 139, 163, 212, 235, 282
GlaI GCGC 4 cut(s) 188, 242, 302, 349
GluI GCNGC 5 cut(s) 139, 163, 212, 235, 282
HaeII RGCGCY 3 cut(s) 244, 304, 351
HaeIII GGCC 8 cut(s) 145, 197, 224, 281, 374, 391, 427, 502
HapII CCGG 4 cut(s) 159, 392, 428, 439
HhaI GCGC 4 cut(s) 189, 243, 303, 350
Hin1II CATG 2 cut(s) 193, 448
Hin6I GCGC 4 cut(s) 187, 241, 301, 348
HinP1I GCGC 4 cut(s) 187, 241, 301, 348
HinfI GANTC 1 cut(s) 561
HpaII CCGG 4 cut(s) 159, 392, 428, 439
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 2 cut(s) 131, 292
Hpy188III TCNNGA 3 cut(s) 319, 385, 439
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 2 cut(s) 320, 463
HpyAV CCTTC 3 cut(s) 52, 163, 358
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4V TGCA 3 cut(s) 193, 490, 592
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 203, 234, 240, 271
HpyF3I CTNAG 1 cut(s) 522
Hsp92II CATG 2 cut(s) 193, 448
HspAI GCGC 4 cut(s) 187, 241, 301, 348
Kpn2I TCCGGA 1 cut(s) 438
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 4 cut(s) 219, 271, 292, 430
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 246
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 2 cut(s) 67, 554
MhlI GDGCHC 3 cut(s) 147, 276, 297
MluCI AATT 2 cut(s) 331, 527
MlyI GAGTC 1 cut(s) 570
MmeI TCCRAC 1 cut(s) 270
MroI TCCGGA 1 cut(s) 438
MseI TTAA 3 cut(s) 99, 107, 584
MslI CAYNNNNRTG 1 cut(s) 131
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 4 cut(s) 159, 392, 428, 439
MspR9I CCNGG 4 cut(s) 148, 159, 307, 393
MvaI CCWGG 2 cut(s) 148, 307
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 5 cut(s) 184, 203, 234, 240, 271
NciI CCSGG 2 cut(s) 159, 393
NcoI CCATGG 1 cut(s) 444
NdeII GATC 1 cut(s) 376
NlaIII CATG 2 cut(s) 193, 448
NlaIV GGNNCC 2 cut(s) 145, 383
NspI RCATGY 1 cut(s) 193
PaeI GCATGC 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 296
PaqCI CACCTGC 1 cut(s) 349
PasI CCCWGGG 1 cut(s) 306
PkrI GCNGC 5 cut(s) 140, 164, 213, 236, 283
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 1 cut(s) 569
PpsI GAGTC 1 cut(s) 569
PpuMI RGGWCCY 2 cut(s) 150, 451
Psp124BI GAGCTC 2 cut(s) 276, 297
Psp5II RGGWCCY 2 cut(s) 150, 451
Psp6I CCWGG 2 cut(s) 146, 305
PspGI CCWGG 2 cut(s) 146, 305
PspN4I GGNNCC 2 cut(s) 145, 383
PspOMI GGGCCC 1 cut(s) 143
PspPI GGNCC 8 cut(s) 143, 144, 150, 223, 411, 425, 441, 451
PspPPI RGGWCCY 2 cut(s) 150, 451
PspXI VCTCGAGB 1 cut(s) 296
PvuI CGATCG 1 cut(s) 379
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 2 cut(s) 276, 297
SaqAI TTAA 3 cut(s) 99, 107, 584
SatI GCNGC 5 cut(s) 139, 163, 212, 235, 282
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 8 cut(s) 143, 144, 150, 223, 411, 425, 441, 451
SchI GAGTC 1 cut(s) 570
ScrFI CCNGG 4 cut(s) 148, 159, 307, 393
SduI GDGCHC 3 cut(s) 147, 276, 297
SfaNI GCATC 1 cut(s) 246
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 4 cut(s) 150, 411, 441, 451
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 4 cut(s) 218, 275, 296, 583
SmoI CTYRAG 4 cut(s) 218, 275, 296, 583
SphI GCATGC 1 cut(s) 193
Sse9I AATT 2 cut(s) 331, 527
SsiI CCGC 8 cut(s) 138, 141, 162, 226, 235, 265, 269, 282
SstI GAGCTC 2 cut(s) 276, 297
StyD4I CCNGG 4 cut(s) 146, 157, 305, 391
StyI CCWWGG 2 cut(s) 444, 477
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 2 cut(s) 297, 318
TaqII GACCGA 1 cut(s) 428
TasI AATT 2 cut(s) 331, 527
TauI GCSGC 4 cut(s) 141, 165, 237, 284
Tru1I TTAA 3 cut(s) 99, 107, 584
Tru9I TTAA 3 cut(s) 99, 107, 584
TscAI CASTG 1 cut(s) 185
TseI GCWGC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 115
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 1 cut(s) 185
Vha464I CTTAAG 1 cut(s) 583
VpaK11BI GGWCC 4 cut(s) 150, 411, 441, 451
XceI RCATGY 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.