RchiOBHm_Chr4g0392921

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005832
Physical Location & Seq
Reverse (-)
8447177 .. 8447519
343 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36557

Sequence Viewer

Length: 342 bp
ATGCGAAAGCGGGCCGAGCTCAAAGCCTCCGTGTACGAGCTCGAGCGCCCCTGGGAGACCGTCGAGGAGCCTCCCAATTTGTTCTCCGTCAGCGCTGATGAGCAGGTTAAGGTCTTGGCATATCCGTTCCGGAGATCGGGAGGGTTTGACTTGTGGACCGAGAGGGATGGGCCGGAGCTGTTTCGGACCATGGAGGATCTGCCATTGACGAGGTTTTTTCCCAAGGGATTTGTGCATAGTGTGAGGCCGTATAGGAGGACTGGGGTTGAGGAATTGGGGGAGGAGGGGGGGGGGGAGGAGAGGGAGAGAGAGAGAGAGAATCTGACTTCAAGAGTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

13.31

Weight (kDa)

5.2

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 94
AccIII TCCGGA 1 cut(s) 129
AciI CCGC 1 cut(s) 10
AclWI GGATC 1 cut(s) 204
AfaI GTAC 1 cut(s) 35
AfeI AGCGCT 1 cut(s) 94
AfiI CCNNNNNNNGG 1 cut(s) 136
AgsI TTSAA 1 cut(s) 330
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 3 cut(s) 19, 40, 178
AluI AGCT 3 cut(s) 19, 40, 178
Alw21I GWGCWC 2 cut(s) 21, 42
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 41
Aor13HI TCCGGA 1 cut(s) 129
Aor51HI AGCGCT 1 cut(s) 94
AoxI GGCC 3 cut(s) 12, 170, 245
AspLEI GCGC 2 cut(s) 48, 95
AspS9I GGNCC 4 cut(s) 12, 156, 170, 186
AvaI CYCGRG 1 cut(s) 41
AvaII GGWCC 2 cut(s) 156, 186
BanII GRGCYC 2 cut(s) 21, 42
Bbv12I GWGCWC 2 cut(s) 21, 42
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 50
BfoI RGCGCY 2 cut(s) 49, 96
BfuAI ACCTGC 1 cut(s) 94
Bme1390I CCNGG 1 cut(s) 52
Bme18I GGWCC 2 cut(s) 156, 186
BmeT110I CYCGRG 1 cut(s) 41
BmgT120I GGNCC 4 cut(s) 12, 156, 170, 186
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 52
BmrI ACTGGG 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 4 cut(s) 50, 51, 189, 222
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse1I ACTGG 1 cut(s) 265
BseAI TCCGGA 1 cut(s) 129
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 4 cut(s) 50, 51, 189, 222
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 136
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 3 cut(s) 80, 296, 311
BshFI GGCC 3 cut(s) 14, 172, 247
BsiHKAI GWGCWC 2 cut(s) 21, 42
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 2 cut(s) 130, 173
BslI CCNNNNNNNGG 1 cut(s) 136
BsmAI GTCTC 1 cut(s) 50
BsnI GGCC 3 cut(s) 14, 172, 247
Bso31I GGTCTC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 21, 42
Bsp13I TCCGGA 1 cut(s) 129
Bsp143I GATC 2 cut(s) 134, 196
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 1 cut(s) 10
BspANI GGCC 3 cut(s) 14, 172, 247
BspEI TCCGGA 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 94
BspPI GGATC 1 cut(s) 204
BspTNI GGTCTC 1 cut(s) 50
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 4 cut(s) 50, 51, 189, 222
BssMI GATC 2 cut(s) 134, 196
BssT1I CCWWGG 2 cut(s) 189, 222
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 12
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 172
BstH2I RGCGCY 2 cut(s) 49, 96
BstHHI GCGC 2 cut(s) 48, 95
BstKTI GATC 2 cut(s) 137, 199
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 2 cut(s) 134, 196
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 3 cut(s) 14, 172, 247
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 1 cut(s) 172
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 1 cut(s) 12
CfoI GCGC 2 cut(s) 48, 95
Cfr13I GGNCC 4 cut(s) 12, 156, 170, 186
Csp6I GTAC 1 cut(s) 34
CviAII CATG 1 cut(s) 190
CviJI RGCY 8 cut(s) 14, 19, 26, 40, 70, 172, 178, 247
CviKI_1 RGCY 8 cut(s) 14, 19, 26, 40, 70, 172, 178, 247
CviQI GTAC 1 cut(s) 34
DpnI GATC 2 cut(s) 136, 198
DpnII GATC 2 cut(s) 134, 196
Ecl136II GAGCTC 2 cut(s) 19, 40
Eco130I CCWWGG 2 cut(s) 189, 222
Eco24I GRGCYC 2 cut(s) 21, 42
Eco31I GGTCTC 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 156, 186
Eco47III AGCGCT 1 cut(s) 94
Eco53kI GAGCTC 2 cut(s) 19, 40
Eco88I CYCGRG 1 cut(s) 41
EcoICRI GAGCTC 2 cut(s) 19, 40
EcoRII CCWGG 1 cut(s) 50
EcoT14I CCWWGG 2 cut(s) 189, 222
EcoT38I GRGCYC 2 cut(s) 21, 42
ErhI CCWWGG 2 cut(s) 189, 222
FaeI CATG 1 cut(s) 193
FaiI YATR 4 cut(s) 121, 191, 237, 252
FatI CATG 1 cut(s) 189
FauI CCCGC 1 cut(s) 3
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 21, 42
GlaI GCGC 2 cut(s) 47, 94
HaeII RGCGCY 2 cut(s) 49, 96
HaeIII GGCC 3 cut(s) 14, 172, 247
HapII CCGG 2 cut(s) 130, 173
HhaI GCGC 2 cut(s) 48, 95
Hin1II CATG 1 cut(s) 193
Hin6I GCGC 2 cut(s) 46, 93
HinP1I GCGC 2 cut(s) 46, 93
HinfI GANTC 1 cut(s) 319
HpaII CCGG 2 cut(s) 130, 173
Hpy166II GTNNAC 2 cut(s) 34, 156
Hpy188I TCNGA 2 cut(s) 186, 324
Hpy188III TCNNGA 3 cut(s) 130, 138, 330
Hpy8I GTNNAC 2 cut(s) 34, 156
Hpy99I CGWCG 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 1 cut(s) 235
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
Hsp92II CATG 1 cut(s) 193
HspAI GCGC 2 cut(s) 46, 93
Kpn2I TCCGGA 1 cut(s) 129
Kzo9I GATC 2 cut(s) 134, 196
LmnI GCTCC 2 cut(s) 67, 175
LpnPI CCDG 6 cut(s) 37, 64, 89, 143, 186, 246
MalI GATC 2 cut(s) 136, 198
MboI GATC 2 cut(s) 134, 196
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 2 cut(s) 21, 42
MluCI AATT 2 cut(s) 76, 272
MroI TCCGGA 1 cut(s) 129
MseI TTAA 1 cut(s) 108
MspI CCGG 2 cut(s) 130, 173
MspR9I CCNGG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 16
NcoI CCATGG 1 cut(s) 189
NdeII GATC 2 cut(s) 134, 196
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 69
NmeAIII GCCGAG 1 cut(s) 40
PaeR7I CTCGAG 1 cut(s) 41
PasI CCCWGGG 1 cut(s) 51
PfeI GAWTC 1 cut(s) 319
Psp124BI GAGCTC 2 cut(s) 21, 42
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 4 cut(s) 12, 156, 170, 186
PspXI VCTCGAGB 1 cut(s) 41
PsuI RGATCY 1 cut(s) 196
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
SacI GAGCTC 2 cut(s) 21, 42
SaqAI TTAA 1 cut(s) 108
Sau3AI GATC 2 cut(s) 134, 196
Sau96I GGNCC 4 cut(s) 12, 156, 170, 186
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 2 cut(s) 21, 42
SetI ASST 6 cut(s) 21, 42, 108, 114, 180, 215
Sfr274I CTCGAG 1 cut(s) 41
SinI GGWCC 2 cut(s) 156, 186
SlaI CTCGAG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 2 cut(s) 76, 272
SsiI CCGC 1 cut(s) 10
SstI GAGCTC 2 cut(s) 21, 42
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 2 cut(s) 189, 222
TaaI ACNGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 42, 63
TaqII GACCGA 1 cut(s) 173
TasI AATT 2 cut(s) 76, 272
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TspGWI ACGGA 3 cut(s) 19, 76, 114
VpaK11BI GGWCC 2 cut(s) 156, 186
XhoI CTCGAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.