Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0002229

Physical Location & Seq

Genomic Coordinates Forward (+)

5302516 .. 5304573

Gene Structure 2058 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 924 bp

ATGGAGGTGGGTGTTGTTGGGTTGCTTAGAACAGCATGGGTTGCAGCGACTATGCCCATACTCGTAGCCGCCGTACATTGTTCTTGGCTGACCTCGTTTCATGGAGCTGTTCTGGGGTTTGCCAAGAGAGGGAAGATCATGGAGTCGTCATCTCAGAAGTTCACAGTTCCTCAAAAGTTGTTCTGCCATTTCTATGTGGTGGCTGTAGCGTGGACAACGCTATTGCTCGTTGCAACTTCGATGTATGCGTATAAAACGGTGCCATTGGTTTCAGAATCGTTGCTTTACCCTTCAATCGCCAGCCACTTCACTGGAGGTTCACATATCTTTTCATGGGACAAGGTTCATTCTGTTCCTGTCAGTTATAGATATAGCATTTGGCGTTCGGTCTTTCTGCTTTTATTGATGGAGGTTCAAGTCTTGAGGCGCCTCTTTGAGACAATATATGTGTTCAACTTTAGCTCCTCCGCTCGGATGCACATTTTCGGATATCTGACTGGCCTCTTCTTCTACACAGCAGCACCACTGTCACTTTGCTGCAATAATGTTCTGGAGGTTTATAAATTTTCAATAAGTGGAGTGGCTGAGTTCATTGTCAAGGGCAAGAGTAGCATGCGAGAAGTGGAATTTGATTGGTTACAATTTGTGAACTCTCTTTTGAAGCTTGGTTGGCTCCAGTGGATTGGTGCAGCTATATTTTTTTGGGGTTGGATCCATCAGCGCCGATGTCATGCAATTCTTGGCTCATTACGAGATCACAGTGAACCAAATGATGAATATGTGATTCCTCATGGTGATTGGTTTGAAATTGTTTCATCTCCACACTATCTGGCTGAGATAGTAAATATGCTGGCCTTGTGGTCGCTAGTGGAGGAACAGATCTCACAATCTGGTTACTTTTTGGATTTGTGGTGTCCAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006066 GO:0006464 GO:0006486 GO:0006487 GO:0006629 GO:0006720 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016093 GO:0016229 GO:0016491 GO:0016627 GO:0019538 GO:0033765 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0070085 GO:0071704 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00140 map00140

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

308

Amino Acids

35.05

Weight (kDa)

7.19

Isoelectric Point (pI)

47.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	224 - 296	2.3e-10	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72590 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT2G16530 AT3G43840 AT3G43840
fragaria_vesca	FvH4_2g10631 FvH4_2g10631 FvH4_2g10631 FvH4_2g10640 FvH4_2g10640 FvH4_2g10640
malus_domestica	MD05G1054700.v1.1 MD10G1062200.v1.1
prunus_persica	Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1 Prupe.8G083500_v2.0.a1
pyrus_communis	pycom05g04490 pycom05g04510 pycom10g04790
rosa_chinensis	RchiOBHm_Chr6g0268311 RchiOBHm_Chr6g0268321 RchiOBHm_Chr6g0268341
rosa_multiflora	Rmu_sc0009812.1_g000007 Rmu_sc0010202.1_g000011
rosa_roxburghii	Rroxscaffold_7G00199860 Rroxscaffold_7G00199880
rosa_rugosa	Rorug06G0040400 Rorug06G0040400 Rorug06G0040400
rosa_samantha	Rh6AG160500 Rh6BG164800 Rh6BG164900 Rh6CG153500 Rh6DG150600
rosa_wichuraiana	Rw0G000500 Rw6G013880 Rw6G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	561
AccB1I	GGYRCC	2 cut(s)	259, 426
AccBSI	CCGCTC	1 cut(s)	470
AciI	CCGC	2 cut(s)	69, 468
AclWI	GGATC	2 cut(s)	706, 719
AcsI	RAATTY	2 cut(s)	563, 626
AcyI	GRCGYC	1 cut(s)	427
AfaI	GTAC	1 cut(s)	75
AfiI	CCNNNNNNNGG	2 cut(s)	129, 471
AgsI	TTSAA	6 cut(s)	294, 416, 454, 570, 661, 806
AluBI	AGCT	4 cut(s)	107, 462, 664, 692
AluI	AGCT	4 cut(s)	107, 462, 664, 692
Alw26I	GTCTC	1 cut(s)	431
AlwI	GGATC	2 cut(s)	706, 719
AoxI	GGCC	2 cut(s)	499, 852
ApeKI	GCWGC	4 cut(s)	44, 518, 537, 689
ApoI	RAATTY	2 cut(s)	563, 626
AspLEI	GCGC	2 cut(s)	429, 723
AsuHPI	GGTGA	1 cut(s)	806
BamHI	GGATCC	1 cut(s)	711
BanI	GGYRCC	2 cut(s)	259, 426
BbvI	GCAGC	4 cut(s)	56, 524, 530, 701
BccI	CCATC	2 cut(s)	400, 723
BceAI	ACGGC	1 cut(s)	56
BcoDI	GTCTC	1 cut(s)	431
BfaI	CTAG	1 cut(s)	866
BfmI	CTRYAG	1 cut(s)	204
BfoI	RGCGCY	2 cut(s)	430, 724
BglII	AGATCT	1 cut(s)	879
BisI	GCNGC	5 cut(s)	45, 69, 519, 538, 690
BlsI	GCNGC	5 cut(s)	46, 70, 520, 539, 691
BmiI	GGNNCC	4 cut(s)	261, 428, 674, 713
BmsI	GCATC	1 cut(s)	465
BpmI	CTGGAG	3 cut(s)	333, 572, 659
BpuEI	CTTGAG	1 cut(s)	442
BsaHI	GRCGYC	1 cut(s)	427
BsaXI	ACNNNNNCTCC	2 cut(s)	446, 476
Bsc4I	CCNNNNNNNGG	2 cut(s)	129, 471
Bse1I	ACTGG	3 cut(s)	316, 502, 676
BseGI	GGATG	1 cut(s)	480
BseLI	CCNNNNNNNGG	2 cut(s)	129, 471
BseMII	CTCAG	3 cut(s)	167, 576, 825
BseNI	ACTGG	3 cut(s)	316, 502, 676
BseRI	GAGGAG	1 cut(s)	454
BseXI	GCAGC	4 cut(s)	56, 524, 530, 701
BsgI	GTGCAG	1 cut(s)	708
BshFI	GGCC	2 cut(s)	501, 854
BshNI	GGYRCC	2 cut(s)	259, 426
BslFI	GGGAC	1 cut(s)	350
BslI	CCNNNNNNNGG	2 cut(s)	129, 471
BsmAI	GTCTC	1 cut(s)	431
BsmFI	GGGAC	1 cut(s)	350
BsnI	GGCC	2 cut(s)	501, 854
Bsp143I	GATC	4 cut(s)	135, 711, 754, 879
BspACI	CCGC	2 cut(s)	69, 468
BspANI	GGCC	2 cut(s)	501, 854
BspCNI	CTCAG	3 cut(s)	166, 577, 826
BspLI	GGNNCC	4 cut(s)	261, 428, 674, 713
BspPI	GGATC	2 cut(s)	706, 719
BspT107I	GGYRCC	2 cut(s)	259, 426
BsrBI	CCGCTC	1 cut(s)	470
BsrI	ACTGG	3 cut(s)	316, 502, 676
BssMI	GATC	4 cut(s)	135, 711, 754, 879
BssNI	GRCGYC	1 cut(s)	427
Bst4CI	ACNGT	4 cut(s)	166, 259, 528, 761
Bst6I	CTCTTC	1 cut(s)	509
BstACI	GRCGYC	1 cut(s)	427
BstAPI	GCANNNNNTGC	1 cut(s)	41
BstC8I	GCNNGC	3 cut(s)	301, 614, 852
BstDEI	CTNAG	5 cut(s)	26, 153, 585, 834, 921
BstF5I	GGATG	1 cut(s)	480
BstH2I	RGCGCY	2 cut(s)	430, 724
BstHHI	GCGC	2 cut(s)	429, 723
BstKTI	GATC	4 cut(s)	138, 714, 757, 882
BstMAI	GTCTC	1 cut(s)	431
BstMBI	GATC	4 cut(s)	135, 711, 754, 879
BstMWI	GCNNNNNNNGC	3 cut(s)	41, 609, 670
BstNSI	RCATGY	1 cut(s)	616
BstSFI	CTRYAG	1 cut(s)	204
BstV1I	GCAGC	4 cut(s)	56, 524, 530, 701
BstX2I	RGATCY	2 cut(s)	711, 879
BstXI	CCANNNNNNTGG	2 cut(s)	311, 683
BstYI	RGATCY	2 cut(s)	711, 879
BsuRI	GGCC	2 cut(s)	501, 854
BtsCI	GGATG	1 cut(s)	480
BtsIMutI	CAGTG	4 cut(s)	309, 524, 683, 766
Cac8I	GCNNGC	3 cut(s)	301, 614, 852
CfoI	GCGC	2 cut(s)	429, 723
Csp6I	GTAC	1 cut(s)	74
CviAII	CATG	7 cut(s)	36, 101, 139, 333, 613, 731, 791
CviQI	GTAC	1 cut(s)	74
DdeI	CTNAG	5 cut(s)	26, 153, 585, 834, 921
DinI	GGCGCC	1 cut(s)	428
DpnI	GATC	4 cut(s)	137, 713, 756, 881
DpnII	GATC	4 cut(s)	135, 711, 754, 879
Eam1104I	CTCTTC	1 cut(s)	509
EarI	CTCTTC	1 cut(s)	509
Eco32I	GATATC	1 cut(s)	491
EcoRV	GATATC	1 cut(s)	491
EgeI	GGCGCC	1 cut(s)	428
EheI	GGCGCC	1 cut(s)	428
FaeI	CATG	7 cut(s)	39, 104, 142, 336, 616, 734, 794
FaqI	GGGAC	1 cut(s)	350
FatI	CATG	7 cut(s)	35, 100, 138, 332, 612, 730, 790
Fnu4HI	GCNGC	5 cut(s)	45, 69, 519, 538, 690
FokI	GGATG	1 cut(s)	487
Fsp4HI	GCNGC	5 cut(s)	45, 69, 519, 538, 690
FspBI	CTAG	1 cut(s)	866
GlaI	GCGC	2 cut(s)	428, 722
GluI	GCNGC	5 cut(s)	45, 69, 519, 538, 690
GsuI	CTGGAG	3 cut(s)	333, 572, 659
HaeII	RGCGCY	2 cut(s)	430, 724
HaeIII	GGCC	2 cut(s)	501, 854
HhaI	GCGC	2 cut(s)	429, 723
Hin1I	GRCGYC	1 cut(s)	427
Hin1II	CATG	7 cut(s)	39, 104, 142, 336, 616, 734, 794
Hin6I	GCGC	2 cut(s)	427, 721
HinP1I	GCGC	2 cut(s)	427, 721
HindIII	AAGCTT	1 cut(s)	662
HinfI	GANTC	3 cut(s)	143, 275, 784
HphI	GGTGA	1 cut(s)	806
Hpy166II	GTNNAC	5 cut(s)	162, 213, 320, 649, 764
Hpy188I	TCNGA	5 cut(s)	156, 274, 474, 488, 495
Hpy188III	TCNNGA	2 cut(s)	421, 551
Hpy8I	GTNNAC	5 cut(s)	162, 213, 320, 649, 764
HpyAV	CCTTC	1 cut(s)	300
HpyCH4III	ACNGT	4 cut(s)	166, 259, 528, 761
HpyCH4V	TGCA	6 cut(s)	44, 233, 478, 540, 689, 734
HpyF10VI	GCNNNNNNNGC	3 cut(s)	41, 609, 670
HpyF3I	CTNAG	5 cut(s)	26, 153, 585, 834, 921
Hsp92I	GRCGYC	1 cut(s)	427
Hsp92II	CATG	7 cut(s)	39, 104, 142, 336, 616, 734, 794
HspAI	GCGC	2 cut(s)	427, 721
KasI	GGCGCC	1 cut(s)	426
Kzo9I	GATC	4 cut(s)	135, 711, 754, 879
LmnI	GCTCC	3 cut(s)	104, 467, 678
Lsp1109I	GCAGC	4 cut(s)	56, 524, 530, 701
LweI	GCATC	1 cut(s)	465
MaeI	CTAG	1 cut(s)	866
MaeIII	GTNAC	3 cut(s)	528, 636, 893
MalI	GATC	4 cut(s)	137, 713, 756, 881
MbiI	CCGCTC	1 cut(s)	470
MboI	GATC	4 cut(s)	135, 711, 754, 879
MboII	GAAGA	3 cut(s)	145, 496, 499
MflI	RGATCY	2 cut(s)	711, 879
MluCI	AATT	5 cut(s)	563, 626, 641, 735, 807
Mly113I	GGCGCC	1 cut(s)	427
MlyI	GAGTC	1 cut(s)	152
MmeI	TCCRAC	1 cut(s)	689
MslI	CAYNNNNRTG	1 cut(s)	192
MwoI	GCNNNNNNNGC	3 cut(s)	41, 609, 670
NarI	GGCGCC	1 cut(s)	427
NdeII	GATC	4 cut(s)	135, 711, 754, 879
NlaIII	CATG	7 cut(s)	39, 104, 142, 336, 616, 734, 794
NlaIV	GGNNCC	4 cut(s)	261, 428, 674, 713
NmuCI	GTSAC	1 cut(s)	528
NspI	RCATGY	1 cut(s)	616
PaeI	GCATGC	1 cut(s)	616
PcsI	WCGNNNNNNNCGW	2 cut(s)	69, 245
PfeI	GAWTC	2 cut(s)	275, 784
PkrI	GCNGC	5 cut(s)	46, 70, 520, 539, 691
PleI	GAGTC	1 cut(s)	151
PluTI	GGCGCC	1 cut(s)	430
PpsI	GAGTC	1 cut(s)	151
PsiI	TTATAA	1 cut(s)	561
PspN4I	GGNNCC	4 cut(s)	261, 428, 674, 713
PsuI	RGATCY	2 cut(s)	711, 879
RsaI	GTAC	1 cut(s)	75
RsaNI	GTAC	1 cut(s)	74
RseI	CAYNNNNRTG	1 cut(s)	192
SatI	GCNGC	5 cut(s)	45, 69, 519, 538, 690
Sau3AI	GATC	4 cut(s)	135, 711, 754, 879
SchI	GAGTC	1 cut(s)	152
SfaNI	GCATC	1 cut(s)	465
SfcI	CTRYAG	1 cut(s)	204
SfoI	GGCGCC	1 cut(s)	428
SmiMI	CAYNNNNRTG	1 cut(s)	192
SmlI	CTYRAG	1 cut(s)	421
SmoI	CTYRAG	1 cut(s)	421
SphI	GCATGC	1 cut(s)	616
Sse9I	AATT	5 cut(s)	563, 626, 641, 735, 807
SsiI	CCGC	2 cut(s)	69, 468
SspDI	GGCGCC	1 cut(s)	426
SspMI	CTAG	1 cut(s)	866
TaaI	ACNGT	4 cut(s)	166, 259, 528, 761
TaqI	TCGA	1 cut(s)	239
TaqII	GACCGA	1 cut(s)	376
TasI	AATT	5 cut(s)	563, 626, 641, 735, 807
TauI	GCSGC	1 cut(s)	71
TfiI	GAWTC	2 cut(s)	275, 784
TscAI	CASTG	4 cut(s)	316, 531, 683, 766
TseFI	GTSAC	1 cut(s)	528
TseI	GCWGC	4 cut(s)	44, 518, 537, 689
Tsp45I	GTSAC	1 cut(s)	528
TspDTI	ATGAA	6 cut(s)	89, 321, 335, 580, 789, 804
TspRI	CASTG	4 cut(s)	316, 531, 683, 766
XapI	RAATTY	2 cut(s)	563, 626
XceI	RCATGY	1 cut(s)	616
XspI	CTAG	1 cut(s)	866

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.