pycom05g10660

Protein trichome birefringence-like 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003034
Physical Location & Seq
Reverse (-)
14112430 .. 14114581
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 726 bp
ATGGAATTGCATCTCCAGCGGCAAATCCAACGGTCGGGAACTACTCAGATGGAGATGGAAGCCCAAGGGTTGATTGATCTACCGCCGTTCCATCCGATTAGGTTTCTGAAGATGTACAGAGACACCAGCATTGGGTTCGTTGGTGATTCATTGAACAGAAATATGTTTGTTTCGCTTTTCTGTTCTCTAAAACGAGTGTCAAGTGAAGTGAAGAAGTGGCGACCAGCTGGTGCTGATCGCGGGTTTACATTTCTTCAGTCTAATCTCACCCTTGCATATCATCGAACAAATCTTTTGGCACGTTATGGTAGGTGGTCAGCCGATGCTAATGGTGGGGTGCTGGAATCTCTTGGATATAAGGAGGGTTATAGAGTTGATGTTGACATTCCAGACGGCACATGGGCAGAAGCTCTAAGCTTTCATGACATTCTGATCTTTAACACGGGGCACTGGTGGTGGGCTCCTTCAAAGTTTGACCCGACAAAGTCACCCATGCTTTTCTTTGAAAAGGGTCGTCCAGTGGTTCCTCCAGTACTCCCTGATGTCGGCCTTGATATGGTTTTGAAGCACATGGTAATGCTCATGGAGAACAGAACGAAAGCAGGTGCAATCAAATTTTTTCGTACTCAGTCACCTAGACATTTTGAAGGGGGTGACTGGGACCAAGGTGGCTCTTGTAATCGGCTAAATCCTTTATTGGTAGAGCAGGTCGAAGATCTCTCCTAA

Protein Analysis

242

Amino Acids

27.54

Weight (kDa)

7.83

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 27 - 237 8.2e-42 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 593
Acc36I ACCTGC 2 cut(s) 593, 697
AccII CGCG 1 cut(s) 240
AciI CCGC 3 cut(s) 19, 83, 240
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 2 cut(s) 128, 239
AfaI GTAC 3 cut(s) 116, 534, 625
AfiI CCNNNNNNNGG 4 cut(s) 34, 132, 545, 556
AgsI TTSAA 5 cut(s) 154, 468, 506, 565, 647
AluBI AGCT 3 cut(s) 227, 410, 417
AluI AGCT 3 cut(s) 227, 410, 417
Alw26I GTCTC 1 cut(s) 114
AoxI GGCC 1 cut(s) 547
ApoI RAATTY 1 cut(s) 614
AspS9I GGNCC 1 cut(s) 661
AsuHPI GGTGA 5 cut(s) 155, 259, 480, 624, 665
AvaII GGWCC 1 cut(s) 661
BaeGI GKGCMC 1 cut(s) 450
BanII GRGCYC 1 cut(s) 463
BccI CCATC 3 cut(s) 43, 49, 99
BceAI ACGGC 2 cut(s) 70, 409
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 636
BfuAI ACCTGC 2 cut(s) 593, 697
BglII AGATCT 1 cut(s) 715
BisI GCNGC 1 cut(s) 20
BlsI GCNGC 1 cut(s) 21
BmcAI AGTACT 1 cut(s) 534
Bme18I GGWCC 1 cut(s) 661
BmgT120I GGNCC 1 cut(s) 661
BmiI GGNNCC 3 cut(s) 462, 525, 662
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 2 cut(s) 19, 313
BmuI ACTGGG 1 cut(s) 667
BpmI CTGGAG 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 64, 664
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 132, 545, 556
Bse1I ACTGG 4 cut(s) 455, 518, 530, 662
BseDI CCNNGG 2 cut(s) 64, 664
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 4 cut(s) 34, 132, 545, 556
BseMII CTCAG 2 cut(s) 59, 641
BseNI ACTGG 4 cut(s) 455, 518, 530, 662
BseSI GKGCMC 1 cut(s) 450
Bsh1236I CGCG 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 4 cut(s) 34, 132, 545, 556
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 674
BsnI GGCC 1 cut(s) 549
Bsp1286I GDGCHC 2 cut(s) 450, 463
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 4 cut(s) 76, 235, 432, 715
BspACI CCGC 3 cut(s) 19, 83, 240
BspANI GGCC 1 cut(s) 549
BspCNI CTCAG 2 cut(s) 58, 640
BspFNI CGCG 1 cut(s) 240
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 3 cut(s) 462, 525, 662
BspMI ACCTGC 2 cut(s) 593, 697
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 4 cut(s) 455, 518, 530, 662
BssECI CCNNGG 2 cut(s) 64, 664
BssMI GATC 4 cut(s) 76, 235, 432, 715
BssT1I CCWWGG 2 cut(s) 64, 664
Bst4CI ACNGT 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 114
BstDEI CTNAG 3 cut(s) 45, 413, 627
BstF5I GGATG 1 cut(s) 91
BstFNI CGCG 1 cut(s) 240
BstKTI GATC 4 cut(s) 79, 238, 435, 718
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 4 cut(s) 76, 235, 432, 715
BstMCI CGRYCG 1 cut(s) 35
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 450
BstUI CGCG 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 1 cut(s) 549
BtsCI GGATG 1 cut(s) 91
BtsIMutI CAGTG 2 cut(s) 448, 525
BveI ACCTGC 2 cut(s) 593, 697
CciI TCATGA 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 661
Csp6I GTAC 3 cut(s) 115, 533, 624
CviAII CATG 5 cut(s) 399, 422, 493, 571, 583
CviJI RGCY 9 cut(s) 62, 227, 320, 410, 417, 461, 549, 672, 685
CviKI_1 RGCY 9 cut(s) 62, 227, 320, 410, 417, 461, 549, 672, 685
CviQI GTAC 3 cut(s) 115, 533, 624
DdeI CTNAG 3 cut(s) 45, 413, 627
DpnI GATC 4 cut(s) 78, 237, 434, 717
DpnII GATC 4 cut(s) 76, 235, 432, 715
Eco130I CCWWGG 2 cut(s) 64, 664
Eco24I GRGCYC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 661
Eco57I CTGAAG 2 cut(s) 128, 239
EcoT14I CCWWGG 2 cut(s) 64, 664
EcoT38I GRGCYC 1 cut(s) 463
ErhI CCWWGG 2 cut(s) 64, 664
FaeI CATG 5 cut(s) 402, 425, 496, 574, 586
FaqI GGGAC 1 cut(s) 674
FatI CATG 5 cut(s) 398, 421, 492, 570, 582
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 1 cut(s) 20
FokI GGATG 1 cut(s) 78
FriOI GRGCYC 1 cut(s) 463
Fsp4HI GCNGC 1 cut(s) 20
FspBI CTAG 1 cut(s) 636
GluI GCNGC 1 cut(s) 20
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 1 cut(s) 549
Hin1II CATG 5 cut(s) 402, 425, 496, 574, 586
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 2 cut(s) 146, 344
HphI GGTGA 5 cut(s) 155, 259, 480, 624, 665
Hpy166II GTNNAC 2 cut(s) 246, 382
Hpy188I TCNGA 4 cut(s) 48, 96, 108, 432
Hpy188III TCNNGA 3 cut(s) 36, 389, 422
Hpy8I GTNNAC 2 cut(s) 246, 382
HpyAV CCTTC 2 cut(s) 474, 641
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4IV ACGT 1 cut(s) 301
HpyCH4V TGCA 3 cut(s) 10, 275, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpyF3I CTNAG 3 cut(s) 45, 413, 627
HpySE526I ACGT 1 cut(s) 301
Hsp92II CATG 5 cut(s) 402, 425, 496, 574, 586
Kzo9I GATC 4 cut(s) 76, 235, 432, 715
LmnI GCTCC 1 cut(s) 466
LweI GCATC 2 cut(s) 19, 313
MaeI CTAG 1 cut(s) 636
MaeII ACGT 1 cut(s) 301
MaeIII GTNAC 3 cut(s) 486, 630, 653
MalI GATC 4 cut(s) 78, 237, 434, 717
MboI GATC 4 cut(s) 76, 235, 432, 715
MboII GAAGA 4 cut(s) 121, 223, 245, 725
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 2 cut(s) 450, 463
MluCI AATT 2 cut(s) 5, 614
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 2 cut(s) 355, 537
MseI TTAA 1 cut(s) 438
MslI CAYNNNNRTG 1 cut(s) 575
MspA1I CMGCKG 2 cut(s) 19, 227
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 16
NdeII GATC 4 cut(s) 76, 235, 432, 715
NlaIII CATG 5 cut(s) 402, 425, 496, 574, 586
NlaIV GGNNCC 3 cut(s) 462, 525, 662
NmuCI GTSAC 3 cut(s) 486, 630, 653
PagI TCATGA 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 146, 344
PflFI GACNNNGTC 1 cut(s) 484
PkrI GCNGC 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 462, 525, 662
PspPI GGNCC 1 cut(s) 661
PsuI RGATCY 1 cut(s) 715
PsyI GACNNNGTC 1 cut(s) 484
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 3 cut(s) 116, 534, 625
RsaNI GTAC 3 cut(s) 115, 533, 624
RseI CAYNNNNRTG 1 cut(s) 575
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 4 cut(s) 76, 235, 432, 715
Sau96I GGNCC 1 cut(s) 661
ScaI AGTACT 1 cut(s) 534
SduI GDGCHC 2 cut(s) 450, 463
SfaNI GCATC 2 cut(s) 19, 313
SinI GGWCC 1 cut(s) 661
SmiMI CAYNNNNRTG 1 cut(s) 575
Sse9I AATT 2 cut(s) 5, 614
SsiI CCGC 3 cut(s) 19, 83, 240
SspMI CTAG 1 cut(s) 636
StyI CCWWGG 2 cut(s) 64, 664
TaaI ACNGT 1 cut(s) 33
TaiI ACGT 1 cut(s) 304
TaqI TCGA 2 cut(s) 283, 711
TasI AATT 2 cut(s) 5, 614
TatI WGTACW 2 cut(s) 114, 532
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 2 cut(s) 146, 344
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TscAI CASTG 2 cut(s) 455, 525
TseFI GTSAC 3 cut(s) 486, 630, 653
Tsp45I GTSAC 3 cut(s) 486, 630, 653
TspDTI ATGAA 2 cut(s) 138, 410
TspRI CASTG 2 cut(s) 455, 525
Tth111I GACNNNGTC 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 661
XapI RAATTY 1 cut(s) 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 396
XspI CTAG 1 cut(s) 636
ZrmI AGTACT 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.